Title:
TARGETED ENRICHMENT AND AMPLIFICATION OF NUCLEIC ACIDS ON A SUPPORT
Document Type and Number:
WIPO Patent Application WO/2013/117595
Kind Code:
A3
Abstract:
Provided herein is a method of selecting target nucleic acids on a support. The method includes providing a plurality of beads each bead comprising one or more oligonucleotides, providing a support with a plurality of primers with a sequence complementary to at least a portion of the oligonucleotides on the beads, contacting the beads with the support wherein the oligonucleotides on the beads bind to the primers on the support, performing an extension reaction by extending the primers on the support to produce capture oligonucleotides, contacting the support comprising the capture oligonucleotides with the target nucleic acids, and extending the capture oligonucleotides bound to target nucleic acids to produce target extension products comprising a sequence complementary to at least a portion of the target nucleic acids. Optionally, the method further includes amplifying the target extension products.
Inventors:
RIGATTI ROBERTO (GB)
BOUTELL JONATHAN MARK (GB)
BOUTELL JONATHAN MARK (GB)
Application Number:
PCT/EP2013/052337
Publication Date:
October 03, 2013
Filing Date:
February 06, 2013
Export Citation:
Assignee:
ILLUMINA CAMBRIDGE LTD (GB)
International Classes:
C12Q1/68
Domestic Patent References:
| WO2010003132A1 | 2010-01-07 | |||
| WO2012040387A1 | 2012-03-29 | |||
| WO2011142861A2 | 2011-11-17 | |||
| WO2005003375A2 | 2005-01-13 |
Foreign References:
| US20090286286A1 | 2009-11-19 | |||
| US20080286795A1 | 2008-11-20 | |||
| US20090124514A1 | 2009-05-14 | |||
| US20050202433A1 | 2005-09-15 | |||
| US20020127569A1 | 2002-09-12 |
Attorney, Agent or Firm:
LEIßLER-GERSTL, Gabriele et al. (München, DE)
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