Title:
TARGETED GENE MODIFICATION BY SINGLE-STRANDED DNA OLIGONUCLEOTIDES
Document Type and Number:
WIPO Patent Application WO2004015117
Kind Code:
A3
Abstract:
A method of site-specifically modifying in a cell a target genomic DNA sequence consisting of first and second DNA strands to introduce a desired alteration, which desired alteration is of a single base-pair or a few base-pairs and is base-pair insertion, deletion, substitution or any combination thereof, the method comprising introducing into the cell a single-stranded targeting DNA molecule, wherein the targeting DNA molecule is 10-100 nucleotides in length and is of nucleotide sequence identical to nucleotide sequence within the first or second DNA strand of the target genomic DNA sequence except for the desired alteration and optionally one or more additional differences, wherein the differences are each insertion, substitution or deletion of a nucleotide, resulting in site-specific modification of the target genomic DNA to introduce the desired alteration.
Inventors:
TE RIELE HENRICUS PETRUS JOSEP (NL)
Application Number:
PCT/IB2003/003699
Publication Date:
June 03, 2004
Filing Date:
August 13, 2003
Export Citation:
Assignee:
NL KANKER I (NL)
TE RIELE HENRICUS PETRUS JOSEP (NL)
TE RIELE HENRICUS PETRUS JOSEP (NL)
International Classes:
C12N15/10; C12N15/90; (IPC1-7): C12N15/10; C12N15/90
Domestic Patent References:
| WO1990014092A1 | 1990-11-29 | |||
| WO1997005268A1 | 1997-02-13 | |||
| WO1993004169A1 | 1993-03-04 | |||
| WO2002026967A2 | 2002-04-04 | |||
| WO2001015740A1 | 2001-03-08 | |||
| WO1996040271A1 | 1996-12-19 | |||
| WO1994004032A1 | 1994-03-03 | |||
| WO2000009755A2 | 2000-02-24 |
Foreign References:
| EP1152058A1 | 2001-11-07 |
Other References:
CAMPBELL C R ET AL: "HOMOLOGOUS RECOMBINATION INVOLVING SMALL SINGLE-STRANDED OLIGONUCLEOTIDES IN HUMAN CELLS", THE NEW BIOLOGIST, PHILADELPHIA,PA, US, vol. 1, no. 2, November 1989 (1989-11-01), pages 223 - 227, XP002933043, ISSN: 1043-4674
MOERSCHELL R P ET AL: "TRANSFORMATION OF YEAST WITH SYNTHETIC OLIGONUCLEOTIDES", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 85, January 1988 (1988-01-01), pages 524 - 528, XP002933044, ISSN: 0027-8424
CULVER KENNETH W ET AL: "Correction of chromosomal point mutations in human cells with bifunctional oligonucleotides", NATURE BIOTECHNOLOGY, NATURE PUBLISHING, US, vol. 17, no. 10, October 1999 (1999-10-01), pages 989 - 993, XP002151266, ISSN: 1087-0156
GAMPER H B ET AL: "Strand invasion of supercoiled DNA by oligonucleotides with a triplex guided sequence", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, AMERICAN CHEMICAL SOCIETY, WASHINGTON, DC, US, vol. 120, 1998, pages 2182 - 2183, XP002151265, ISSN: 0002-7863
MOERSCHELL R P ET AL: "TRANSFORMATION OF YEAST WITH SYNTHETIC OLIGONUCLEOTIDES", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 85, January 1988 (1988-01-01), pages 524 - 528, XP002933044, ISSN: 0027-8424
CULVER KENNETH W ET AL: "Correction of chromosomal point mutations in human cells with bifunctional oligonucleotides", NATURE BIOTECHNOLOGY, NATURE PUBLISHING, US, vol. 17, no. 10, October 1999 (1999-10-01), pages 989 - 993, XP002151266, ISSN: 1087-0156
GAMPER H B ET AL: "Strand invasion of supercoiled DNA by oligonucleotides with a triplex guided sequence", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, AMERICAN CHEMICAL SOCIETY, WASHINGTON, DC, US, vol. 120, 1998, pages 2182 - 2183, XP002151265, ISSN: 0002-7863
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