Title:
CRISPR/CAS9-TARGETED KNOCKOUT OF HUMAN TP55 GENE AND SPECIFIC GRNA THEREFOR
Document Type and Number:
WIPO Patent Application WO/2020/000459
Kind Code:
A1
Abstract:
Provided are a method for CRISPR/Cas9-targeted knockout of a human Tp55 gene, and specific gRNA therefor. The method comprises: designing two target sites at upstream and downstream of human Tp55 gene according to the design principle of CRISPR/Cas9, synthesizing a corresponding oligonucleotide sequence, connecting the sequence to a px459 vector to construct a recombinant plasmid, and transfecting the recombinant plasmid to a human esophageal carcinoma cell strain to specifically knock the human Tp55 gene out.
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Inventors:
MAO JIYAN (CN)
Application Number:
PCT/CN2018/093869
Publication Date:
January 02, 2020
Filing Date:
June 29, 2018
Export Citation:
Assignee:
SHENZHEN BIOCAN TECH CO LTD (CN)
International Classes:
C12N15/113
Foreign References:
| CN106868008A | 2017-06-20 |
Other References:
DATABASE Genbank 29 April 2018 (2018-04-29), TEPLYAKOV, A. ET AL.: "Homo sapiens CD 27 molecule ( CD 27), mRNA", XP055669223, Database accession no. NM_001242.4
VAN, L. R. A. ET AL.: "Tissue distribution and biochemical and functional properties of Tp55 ( CD 27), a novel T cell differentiation antigen", J IMMUNOL., vol. 139, no. 5, 1 September 1987 (1987-09-01), XP002467371, ISSN: 0022-1767
YU , JUNYAO ET AL.: "CRISPR-Cas (Non-official translation: Research Progress and Application of CRISPR-Cas9 System", LETTERS IN BIOTECHNOLOGY, vol. 29, no. 3, 30 May 2018 (2018-05-30), ISSN: 1009-0002
VAN, L. R. A. ET AL.: "Tissue distribution and biochemical and functional properties of Tp55 ( CD 27), a novel T cell differentiation antigen", J IMMUNOL., vol. 139, no. 5, 1 September 1987 (1987-09-01), XP002467371, ISSN: 0022-1767
YU , JUNYAO ET AL.: "CRISPR-Cas (Non-official translation: Research Progress and Application of CRISPR-Cas9 System", LETTERS IN BIOTECHNOLOGY, vol. 29, no. 3, 30 May 2018 (2018-05-30), ISSN: 1009-0002
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