Title:
TARGETED PHOTODYNAMIC THERAPY FOR DISEASE ASSOCIATED WITH SARS-COV-2
Document Type and Number:
WIPO Patent Application WO/2023/143582
Kind Code:
A1
Abstract:
Disclosed is a fusion protein comprising: (i) an antibody fragment; (ii) a linker fragment; and (iii) a photosensitive protein; wherein the antibody fragment binds with the photosensitive protein by means of the linker fragment, the antibody fragment specifically binds to a virus, and under the illumination of light of a specific wavelength, the photosensitive protein generates reactive oxygen molecules, especially singlet oxygen, and consequently kills or damages the virus in a targeted manner, causing the virus to lose the ability to infect a host cell.
Inventors:
ZHOU LEI (CN)
YAO RUHUI (CN)
HOU JIAN (CN)
ZHANG XIN (CN)
YAO RUHUI (CN)
HOU JIAN (CN)
ZHANG XIN (CN)
Application Number:
PCT/CN2023/073728
Publication Date:
August 03, 2023
Filing Date:
January 29, 2023
Export Citation:
Assignee:
SHENZHEN BAY LABORATORY (CN)
International Classes:
C07K16/08; A61K41/00; A61N5/06; C12N15/62; A61P31/00; A61P35/00
Foreign References:
| CN110658190A | 2020-01-07 | |||
| US20190275345A1 | 2019-09-12 |
Other References:
SEREBROVSKAYA, E.O. ET AL.: "Targeting Cancer Cells by Using an Antireceptor Antibody-Photosensitizer Fusion Protein", PNAS, vol. 106, no. 23, 9 June 2009 (2009-06-09), XP055622034, DOI: 10.1073/pnas.0904140106
SADRAEIAN MOHAMMAD, BAHOU CALISE, DA CRUZ EDGAR FERREIRA, JANINI LUÍZ MÁRIO RAMOS, SOBHIE DIAZ RICARDO, BOYLE ROSS W., CHUDASAMA V: "Photoimmunotherapy Using Cationic and Anionic Photosensitizer-Antibody Conjugates against HIV Env-Expressing Cells", INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 21, no. 23, 1 December 2020 (2020-12-01), pages 9151, XP093080456, DOI: 10.3390/ijms21239151
SADRAEIAN MOHAMMAD, BAHOU CALISE, DA CRUZ EDGAR FERREIRA, JANINI LUÍZ MÁRIO RAMOS, SOBHIE DIAZ RICARDO, BOYLE ROSS W., CHUDASAMA V: "Photoimmunotherapy Using Cationic and Anionic Photosensitizer-Antibody Conjugates against HIV Env-Expressing Cells", INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 21, no. 23, 1 December 2020 (2020-12-01), pages 9151, XP093080456, DOI: 10.3390/ijms21239151
SADRAEIAN MOHAMMAD, DA CRUZ EDGAR FERREIRA, BOYLE ROSS W., BAHOU CALISE, CHUDASAMA VIJAY, JANINI LUIZ MÁRIO RAMOS, DIAZ RICARDO SO: "Photoinduced Photosensitizer–Antibody Conjugates Kill HIV Env-Expressing Cells, Also Inactivating HIV", ACS OMEGA, ACS PUBLICATIONS, US, vol. 6, no. 25, 29 June 2021 (2021-06-29), US , pages 16524 - 16534, XP093080460, ISSN: 2470-1343, DOI: 10.1021/acsomega.1c01721
YAO RUHUI, HOU JIAN, ZHANG XIN, LI YI, LAI JUNHUI, WU QINQIN, LIU QINGLIAN, ZHOU LEI: "Targeted photodynamic neutralization of SARS-CoV-2 mediated by singlet oxygen", PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, ROYAL SOCIETY OF CHEMISTRY , CAMBRIDGE, GB, vol. 22, no. 6, 1 June 2023 (2023-06-01), GB , pages 1323 - 1340, XP093080461, ISSN: 1474-905X, DOI: 10.1007/s43630-023-00381-w
PAI JAMES J.-K., KIRKUP MICHAEL P., FRANK EMILY A., PACHTER JONATHAN A., BRYANT ROBERT W.: "Compounds Capable of Generating Singlet Oxygen Represent a Source of Artifactual Data in Scintillation Proximity Assays Measuring Phosphopeptide Binding to SH2 Domains: Volume 270, Number 1 (1999), pages 33–40", ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS, AMSTERDAM, NL, vol. 276, no. 2, 15 December 1999 (1999-12-15), Amsterdam, NL , pages 262 - 262, XP093080462, ISSN: 0003-2697, DOI: 10.1006/abio.1999.4340
LIANG LIUEN, CARE ANDREW, ZHANG RUN, LU YIQING, PACKER NICOLLE H., SUNNA ANWAR, QIAN YI, ZVYAGIN ANDREI V.: "Facile Assembly of Functional Upconversion Nanoparticles for Targeted Cancer Imaging and Photodynamic Therapy", APPLIED MATERIALS & INTERFACES, AMERICAN CHEMICAL SOCIETY, US, vol. 8, no. 19, 18 May 2016 (2016-05-18), US , pages 11945 - 11953, XP093080463, ISSN: 1944-8244, DOI: 10.1021/acsami.6b00713
HILGERS FABIENNE, BITZENHOFER NORA LISA, ACKERMANN YANNIC, BURMEISTER ALINA, GRÜNBERGER ALEXANDER, JAEGER KARL-ERICH, DREPPER THOM: "Genetically Encoded Photosensitizers as Light-Triggered Antimicrobial Agents", INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 20, no. 18, 17 September 2019 (2019-09-17), pages 4608, XP093080465, DOI: 10.3390/ijms20184608
SADRAEIAN MOHAMMAD, BAHOU CALISE, DA CRUZ EDGAR FERREIRA, JANINI LUÍZ MÁRIO RAMOS, SOBHIE DIAZ RICARDO, BOYLE ROSS W., CHUDASAMA V: "Photoimmunotherapy Using Cationic and Anionic Photosensitizer-Antibody Conjugates against HIV Env-Expressing Cells", INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 21, no. 23, 1 December 2020 (2020-12-01), pages 9151, XP093080456, DOI: 10.3390/ijms21239151
SADRAEIAN MOHAMMAD, BAHOU CALISE, DA CRUZ EDGAR FERREIRA, JANINI LUÍZ MÁRIO RAMOS, SOBHIE DIAZ RICARDO, BOYLE ROSS W., CHUDASAMA V: "Photoimmunotherapy Using Cationic and Anionic Photosensitizer-Antibody Conjugates against HIV Env-Expressing Cells", INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 21, no. 23, 1 December 2020 (2020-12-01), pages 9151, XP093080456, DOI: 10.3390/ijms21239151
SADRAEIAN MOHAMMAD, DA CRUZ EDGAR FERREIRA, BOYLE ROSS W., BAHOU CALISE, CHUDASAMA VIJAY, JANINI LUIZ MÁRIO RAMOS, DIAZ RICARDO SO: "Photoinduced Photosensitizer–Antibody Conjugates Kill HIV Env-Expressing Cells, Also Inactivating HIV", ACS OMEGA, ACS PUBLICATIONS, US, vol. 6, no. 25, 29 June 2021 (2021-06-29), US , pages 16524 - 16534, XP093080460, ISSN: 2470-1343, DOI: 10.1021/acsomega.1c01721
YAO RUHUI, HOU JIAN, ZHANG XIN, LI YI, LAI JUNHUI, WU QINQIN, LIU QINGLIAN, ZHOU LEI: "Targeted photodynamic neutralization of SARS-CoV-2 mediated by singlet oxygen", PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, ROYAL SOCIETY OF CHEMISTRY , CAMBRIDGE, GB, vol. 22, no. 6, 1 June 2023 (2023-06-01), GB , pages 1323 - 1340, XP093080461, ISSN: 1474-905X, DOI: 10.1007/s43630-023-00381-w
PAI JAMES J.-K., KIRKUP MICHAEL P., FRANK EMILY A., PACHTER JONATHAN A., BRYANT ROBERT W.: "Compounds Capable of Generating Singlet Oxygen Represent a Source of Artifactual Data in Scintillation Proximity Assays Measuring Phosphopeptide Binding to SH2 Domains: Volume 270, Number 1 (1999), pages 33–40", ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS, AMSTERDAM, NL, vol. 276, no. 2, 15 December 1999 (1999-12-15), Amsterdam, NL , pages 262 - 262, XP093080462, ISSN: 0003-2697, DOI: 10.1006/abio.1999.4340
LIANG LIUEN, CARE ANDREW, ZHANG RUN, LU YIQING, PACKER NICOLLE H., SUNNA ANWAR, QIAN YI, ZVYAGIN ANDREI V.: "Facile Assembly of Functional Upconversion Nanoparticles for Targeted Cancer Imaging and Photodynamic Therapy", APPLIED MATERIALS & INTERFACES, AMERICAN CHEMICAL SOCIETY, US, vol. 8, no. 19, 18 May 2016 (2016-05-18), US , pages 11945 - 11953, XP093080463, ISSN: 1944-8244, DOI: 10.1021/acsami.6b00713
HILGERS FABIENNE, BITZENHOFER NORA LISA, ACKERMANN YANNIC, BURMEISTER ALINA, GRÜNBERGER ALEXANDER, JAEGER KARL-ERICH, DREPPER THOM: "Genetically Encoded Photosensitizers as Light-Triggered Antimicrobial Agents", INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 20, no. 18, 17 September 2019 (2019-09-17), pages 4608, XP093080465, DOI: 10.3390/ijms20184608
Attorney, Agent or Firm:
SHENZHEN ZHIHUIYUANJIAN INTELLECTUAL PROPERTY AGENCY CO., LTD. (CN)
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