Title:
TARGETED RNA EDITING BY LEVERAGING ENDOGENOUS ADAR USING ENGINEERED RNAS
Document Type and Number:
WIPO Patent Application WO/2021/008447
Kind Code:
A1
Abstract:
Provided are methods for editing RNA by introducing a deaminase-recruiting RNA in a host cell for deamination of an adenosine in a target RNA, deaminase-recruiting RNAs used in the RNA editing methods, compositions and kits comprising the same.
Inventors:
WEI WENSHENG (CN)
YI ZONGYI (CN)
QU LIANG (CN)
TIAN FENG (CN)
WANG CHUNHUI (CN)
ZHU SHIYOU (CN)
ZHOU ZHUO (CN)
YI ZONGYI (CN)
QU LIANG (CN)
TIAN FENG (CN)
WANG CHUNHUI (CN)
ZHU SHIYOU (CN)
ZHOU ZHUO (CN)
Application Number:
PCT/CN2020/101246
Publication Date:
January 21, 2021
Filing Date:
July 10, 2020
Export Citation:
Assignee:
UNIV BEIJING (CN)
International Classes:
C12N15/113; A61K48/00; C12N15/00; C12N15/09
Domestic Patent References:
| WO2016097212A1 | 2016-06-23 |
Other References:
LITKE，J.L.ET AL.: "Highly efficient expression of circular RNA aptamers in cells using autocatalytic transcripts.", NAT BIOTECHNOL., vol. 37, no. 6, 8 April 2019 (2019-04-08), pages 667 - 675, XP036797540, DOI: 20200921133952Y
YIN，Q.F.ET AL.: "Long Noncoding RNAs with snoRNA Ends.", MOL CELL., vol. 48, no. 2, 6 September 2012 (2012-09-06), pages 219 - 230, XP028953204, DOI: 20200921141236Y
WANG，Y.ET AL.: "A Phenotypic Screen for Functional Mutants of Human Adenosine Deaminase acting on RNA 1.", ACS CHEM BIOL., vol. 10, no. 11, 22 September 2015 (2015-09-22), pages 2512 - 2519, XP055774020, DOI: 20200921141550Y
QU，L.ET AL.: "Programmable RNA editing by recruiting endogenous ADAR using engineered RNAs.", NAT BIOTECHNOL., vol. 37, no. 9, 15 July 2019 (2019-07-15), pages 1059 - 1069, XP036878168, DOI: 20200921142035PX
ZHENG，Y.ET AL.: "DNA editing in DNA/RNA hybrids by adenosine deaminases that act on RNA.", NUCLEIC ACIDS RES., vol. 45, no. 6, 28 January 2017 (2017-01-28), pages 3369 - 3377, XP055404026, DOI: 20200921141747A
YIN，Q.F.ET AL.: "Long Noncoding RNAs with snoRNA Ends.", MOL CELL., vol. 48, no. 2, 6 September 2012 (2012-09-06), pages 219 - 230, XP028953204, DOI: 20200921141236Y
WANG，Y.ET AL.: "A Phenotypic Screen for Functional Mutants of Human Adenosine Deaminase acting on RNA 1.", ACS CHEM BIOL., vol. 10, no. 11, 22 September 2015 (2015-09-22), pages 2512 - 2519, XP055774020, DOI: 20200921141550Y
QU，L.ET AL.: "Programmable RNA editing by recruiting endogenous ADAR using engineered RNAs.", NAT BIOTECHNOL., vol. 37, no. 9, 15 July 2019 (2019-07-15), pages 1059 - 1069, XP036878168, DOI: 20200921142035PX
ZHENG，Y.ET AL.: "DNA editing in DNA/RNA hybrids by adenosine deaminases that act on RNA.", NUCLEIC ACIDS RES., vol. 45, no. 6, 28 January 2017 (2017-01-28), pages 3369 - 3377, XP055404026, DOI: 20200921141747A
Attorney, Agent or Firm:
BEIJING CAIHE LAW FIRM (CN)
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