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Title:
TARGETING EPIGENETIC REGULATORS USING A BACTERIAL DELIVERY SYSTEM
Document Type and Number:
WIPO Patent Application WO/2016/025582
Kind Code:
A3
Abstract:
One embodiment provides an attenuated Salmonella strain comprising a lysis gene or cassette operably linked to an intracellularly induced Salmonella promoter. In one embodiment, the promoter is a promoter for one of the genes in Salmonella pathogenicity island 2 type III secretion system (SPI2-T3SS) selected from the group SpiC/SsaB, SseF, SseG, SseI, SseJ, SseK1, SseK2, SifA, SifB, PipB, PipB2, SopD2, GogB, SseL, SteC, SspH1, SspH2, or SirP. In one embodiment, a Salmonella gene is under the regulation of an inducible promoter, wherein the gene is selected from ftsW, ftsA, ftsZ, murE, mukF, imp, secF, eno, hemH, tmk, dxs, uppS, cdsA, accA, pssA, msbA, tsf, trmD, cca, infB, rpoA, rpoB, rpoC, holA, dnaC, or eng. In one embodiment, the Salmonella strain further comprises a plasmid that expresses DNA, shRNA, non-coding RNA and/or a peptide.

Inventors:
FORBES NEIL S (US)
VAN DESSEL NELE (US)
Application Number:
PCT/US2015/044831
Publication Date:
April 07, 2016
Filing Date:
August 12, 2015
Export Citation:
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Assignee:
FORBES NEIL S (US)
VAN DESSEL NELE (US)
International Classes:
A61K39/112; C12N1/21
Domestic Patent References:
WO2013124425A12013-08-29
Foreign References:
US20080112928A12008-05-15
US20050118193A12005-06-02
US20060140975A12006-06-29
US20090208534A12009-08-20
US20130295054A12013-11-07
Other References:
XU ET AL.: "Efficacy of intracellular activated promoters for generation of Salmonella-based vaccines", INFECT IMMUN., vol. 78, 23 August 2010 (2010-08-23), pages 4828 - 483 8
KNUTH ET AL.: "Large-scale identification of essential Salmonella genes by trapping lethal insertions", MOL MICROBIOL., vol. 51, March 2004 (2004-03-01), pages 1729 - 1744
Attorney, Agent or Firm:
PERDOK, Monique M. et al. (Minneapolis, Minnesota, US)
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