Title:
TECHNICALLY PRODUCED DIHYDROCOUMARIN
Document Type and Number:
WIPO Patent Application WO2006015811
Kind Code:
A3
Abstract:
The invention relates to a dihydrocoumarin that has been technically produced by the biotransformation of coumarin and to associated production variants that are carried out with the aid of isolated enzymes and/or microorganisms. Pure coumarin, coumarin that has been isolated from a plant extract, or plant extracts containing coumarin can be used as the parent materials. Selected strains of Saccharomyces, Arthrobacter, Pseudomonas, Bacillus, Basidiomycetes and Fusarium can be directly used for the biotransformation or can provide the enzymes required for said biotransformation. Preferred enzymes are coumarate and coumarin reductases. The invention also relates to a production variant, which is based on coumarin and which by means of either o-coumaric acid, or by means of an intermediately formed dihydrocoumarin produces melilotic acid, which is subsequently dehydrated to form dihydrocoumarin. The dihydrocoumarin thus obtained fulfils all the criteria of a natural raw material and for this reason can be used as a natural flavouring, for producing natural, nature-identical and synthetic flavourings, or can be used as a component of such flavourings, which are added to baked goods, sweets, beverages, cremes, cereal products and milk products.
Inventors:
WENK HANS HENNING (DE)
SCHWAB WILFRIED (DE)
HAESER KATRIN (DE)
SCHWAB WILFRIED (DE)
HAESER KATRIN (DE)
Application Number:
PCT/EP2005/008512
Publication Date:
April 06, 2006
Filing Date:
August 05, 2005
Export Citation:
Assignee:
MAXENS GMBH (DE)
WENK HANS HENNING (DE)
SCHWAB WILFRIED (DE)
HAESER KATRIN (DE)
WENK HANS HENNING (DE)
SCHWAB WILFRIED (DE)
HAESER KATRIN (DE)
International Classes:
A23L27/00; A23L7/10; C12P17/06; A23L27/20; A23L27/24; C07D311/20; C12P7/42
Foreign References:
| US6462203B2 | 2002-10-08 | |||
| US5874398A | 1999-02-23 | |||
| US3442910A | 1969-05-06 |
Other References:
KOSUGE T ET AL: "The metabolism of aromatic compounds in higher plants. V. Purification and properties of dihydrocoumarin hydrolase of Melilotus alba.", THE JOURNAL OF BIOLOGICAL CHEMISTRY. MAY 1962, vol. 237, May 1962 (1962-05-01), pages 1653 - 1656, XP002365187, ISSN: 0021-9258
LEVY C C ET AL: "THE METABOLISM OF COUMARIN BY A MICROORGANISM. II. THE REDUCTION OF O-COUMARIC ACID TO MELILOTIC ACID.", BIOCHEMISTRY. DEC 1964, vol. 3, December 1964 (1964-12-01), pages 1944 - 1947, XP002365188, ISSN: 0006-2960
NAKAYAMA Y ET AL: "THE METABOLISM OF COUMARIN BY A PSEUDOMONAS-SP", AGRICULTURAL AND BIOLOGICAL CHEMISTRY, vol. 37, no. 6, 1973, pages 1423 - 1437, XP008059131, ISSN: 0002-1369
SHIEH H S ET AL: "Use of coumarin by soil fungi.", CANADIAN JOURNAL OF MICROBIOLOGY. JUN 1969, vol. 15, no. 6, June 1969 (1969-06-01), pages 647 - 648, XP008059134, ISSN: 0008-4166
LEVY C C ET AL: "THE METABOLISM OF COUMARIN BY A MICROORGANISM. II. THE REDUCTION OF O-COUMARIC ACID TO MELILOTIC ACID.", BIOCHEMISTRY. DEC 1964, vol. 3, December 1964 (1964-12-01), pages 1944 - 1947, XP002365188, ISSN: 0006-2960
NAKAYAMA Y ET AL: "THE METABOLISM OF COUMARIN BY A PSEUDOMONAS-SP", AGRICULTURAL AND BIOLOGICAL CHEMISTRY, vol. 37, no. 6, 1973, pages 1423 - 1437, XP008059131, ISSN: 0002-1369
SHIEH H S ET AL: "Use of coumarin by soil fungi.", CANADIAN JOURNAL OF MICROBIOLOGY. JUN 1969, vol. 15, no. 6, June 1969 (1969-06-01), pages 647 - 648, XP008059134, ISSN: 0008-4166
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