Title:
TEMPERATURE RESPONSIVE SHEET THAT DISPLAYS REVERSIBLE PROPERTIES AND CELL SHEET PRODUCTION METHOD USING SAME
Document Type and Number:
WIPO Patent Application WO/2011/155565
Kind Code:
A1
Abstract:
Provided is a method that produces a temperature responsive sheet that is derived from an organism, has extremely low toxicity and exhibits reversible properties. Said properties are used to recover sheets of cultured cells intact. Provided is a temperature responsive sheet formed from a chemically modified water-soluble elastin obtained by the N-acylation of at least some of the first amines and the second amines in the molecule of a high molecular weight water-soluble elastin, and the coupling of some or all of the carboxyl groups in said molecule with the alkyl ether of an amino acid. Also provided is a production method for a cell sheet that is characterised by: the manufacture of a film that forms a scaffold for animal cells on the temperature responsive sheet; the culturing of specific cells on the film and manufacture of a cell sheet; and the separation of the cell sheet and the temperature responsive sheet, which is formed from the chemically modified water-soluble elastin, at a temperature at or below the cell culturing temperature.
Inventors:
OKAMOTO KOUJI (JP)
YOSHINO ERIKA (JP)
YOSHINO ERIKA (JP)
Application Number:
PCT/JP2011/063265
Publication Date:
December 15, 2011
Filing Date:
June 09, 2011
Export Citation:
Assignee:
KYUSHU INST TECHNOLOGY (JP)
OKAMOTO KOUJI (JP)
YOSHINO ERIKA (JP)
OKAMOTO KOUJI (JP)
YOSHINO ERIKA (JP)
International Classes:
C12M1/00; C07K14/78; A61K35/12
Domestic Patent References:
| WO2006046626A1 | 2006-05-04 |
Other References:
MIE, M. ET AL.: "Novel extracellular matrix for cell sheet recovery using genetically engineered elastin-like protein", JOURNAL OF BIOMEDICAL MATERIALS RESEARCH. PART B. APPLIED BIOMATERIALS, vol. 86, no. 1, 2007, pages 283 - 290
YASUNORI MIZUSHIMA ET AL.: "Kokino Saibo Sheet Kochiku no Tameno Jinko Tanpakushitsu Zairyo no Kaihatsu", THE CHEMICAL SOCIETY OF JAPAN DAI 86 SHUNKI NENKAI KOEN YOKOSHU II, 2006, pages 899, 4G4-11
YOSUKE MAEKAWA ET AL.: "Kagaku Shushoku Suiyosei Elastin Oyobi I-gata Collagen Kyozon Jotai ni Okeru Jiko Shugo Tokusei", KITAKYUSHU IKO GAKUJUTSUSHA KYOKAISHI, vol. 20, April 2010 (2010-04-01), pages 25 - 28
YASUNORI MIZUSHIMA ET AL.: "Kokino Saibo Sheet Kochiku no Tameno Jinko Tanpakushitsu Zairyo no Kaihatsu", THE CHEMICAL SOCIETY OF JAPAN DAI 86 SHUNKI NENKAI KOEN YOKOSHU II, 2006, pages 899, 4G4-11
YOSUKE MAEKAWA ET AL.: "Kagaku Shushoku Suiyosei Elastin Oyobi I-gata Collagen Kyozon Jotai ni Okeru Jiko Shugo Tokusei", KITAKYUSHU IKO GAKUJUTSUSHA KYOKAISHI, vol. 20, April 2010 (2010-04-01), pages 25 - 28
Attorney, Agent or Firm:
NOGUCHI YASUHIRO (JP)
Takahiro Noguchi (JP)
Takahiro Noguchi (JP)
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Claims: