Title:
TFPI BINDING POLYPEPTIDES AND USES THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/143559
Kind Code:
A1
Abstract:
The invention provides polypeptides that bind Tissue Factor Pathway Inhibitor (TFPI), including TFPI-inhibitory polypeptides, and compositions thereof. The polypeptides may be used to inhibit a TFPI, enhance thrombin formation in a clotting factor-deficient subject, increase blood clot formation in a subject, treat a blood coagulation disorder in a subject, purify TFPI, and identify a TFPI-binding compound, among others.
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Inventors:
TAO LIANG (CN)
LI YANYAN (CN)
LUO JIANHUA (CN)
YANG QI (CN)
LI YANYAN (CN)
LUO JIANHUA (CN)
YANG QI (CN)
Application Number:
PCT/CN2023/073679
Publication Date:
August 03, 2023
Filing Date:
January 29, 2023
Export Citation:
Assignee:
UNIV WESTLAKE (CN)
International Classes:
C07K14/195; A61K38/16; A61P7/00; C12N5/00
Foreign References:
| US20150044250A1 | 2015-02-12 | |||
| US20150093389A1 | 2015-04-02 | |||
| US20120028901A1 | 2012-02-02 | |||
| US20110318356A1 | 2011-12-29 |
Other References:
CHEN PENG; LAM KWOK-HO; LIU ZHENG; MINDLIN FRANK A.; CHEN BAOHUA; GUTIERREZ CRAIG B.; HUANG LAN; ZHANG YONGRONG; HAMZA THERWA; FEN: "Structure of the full-lengthClostridium difficiletoxin B", NATURE STRUCTURAL & MOLECULAR BIOLOGY, NATURE PUBLISHING GROUP US, NEW YORK, vol. 26, no. 8, 15 July 2019 (2019-07-15), New York , pages 712 - 719, XP036852000, ISSN: 1545-9993, DOI: 10.1038/s41594-019-0268-0
DATABASE UniProtKB/Swiss-Prot 29 September 2021 (2021-09-29), ANONYMOUS : "RecName: Full=Toxin B {ECO:0000303", XP093081930, Database accession no. Q9EXR0
DATABASE Protein 26 March 2021 (2021-03-26), ANONYMOUS : "glycosylating toxin TcdB [Clostridioides difficile]", XP093081021, retrieved from NCBI Database accession no. WP_009888442.1
LUO JIANHUA; YANG QI; ZHANG XIAOFENG; ZHANG YUANYUAN; WAN LI; ZHAN XIECHAO; ZHOU YAO; HE LIUQING; LI DANYANG; JIN DAZHI; ZHEN YING: "TFPI is a colonic crypt receptor for TcdB from hypervirulent clade 2 C. difficile", CELL, ELSEVIER, AMSTERDAM NL, vol. 185, no. 6, 17 March 2022 (2022-03-17), Amsterdam NL , pages 980, XP086998459, ISSN: 0092-8674, DOI: 10.1016/j.cell.2022.02.010
AKTORIES KLAUS: "Another surprise in receptor binding of C. difficile toxins", THE INNOVATION, vol. 3, no. 4, 1 July 2022 (2022-07-01), pages 100261, XP093081023, ISSN: 2666-6758, DOI: 10.1016/j.xinn.2022.100261
TIAN SONGHAI, XIONG XIAOZHE, ZENG JI, WANG SIYU, TREMBLAY BENJAMIN JEAN-MARIE, CHEN PENG, CHEN BAOHUA, LIU MIN, CHEN PENGSHENG, SH: "Identification of TFPI as a receptor reveals recombination-driven receptor switching in Clostridioides difficile toxin B variants", NATURE COMMUNICATIONS, vol. 13, no. 1, 9 November 2022 (2022-11-09), pages 6786, XP093081024, DOI: 10.1038/s41467-022-33964-9
DATABASE UniProtKB/Swiss-Prot 29 September 2021 (2021-09-29), ANONYMOUS : "RecName: Full=Toxin B {ECO:0000303", XP093081930, Database accession no. Q9EXR0
DATABASE Protein 26 March 2021 (2021-03-26), ANONYMOUS : "glycosylating toxin TcdB [Clostridioides difficile]", XP093081021, retrieved from NCBI Database accession no. WP_009888442.1
LUO JIANHUA; YANG QI; ZHANG XIAOFENG; ZHANG YUANYUAN; WAN LI; ZHAN XIECHAO; ZHOU YAO; HE LIUQING; LI DANYANG; JIN DAZHI; ZHEN YING: "TFPI is a colonic crypt receptor for TcdB from hypervirulent clade 2 C. difficile", CELL, ELSEVIER, AMSTERDAM NL, vol. 185, no. 6, 17 March 2022 (2022-03-17), Amsterdam NL , pages 980, XP086998459, ISSN: 0092-8674, DOI: 10.1016/j.cell.2022.02.010
AKTORIES KLAUS: "Another surprise in receptor binding of C. difficile toxins", THE INNOVATION, vol. 3, no. 4, 1 July 2022 (2022-07-01), pages 100261, XP093081023, ISSN: 2666-6758, DOI: 10.1016/j.xinn.2022.100261
TIAN SONGHAI, XIONG XIAOZHE, ZENG JI, WANG SIYU, TREMBLAY BENJAMIN JEAN-MARIE, CHEN PENG, CHEN BAOHUA, LIU MIN, CHEN PENGSHENG, SH: "Identification of TFPI as a receptor reveals recombination-driven receptor switching in Clostridioides difficile toxin B variants", NATURE COMMUNICATIONS, vol. 13, no. 1, 9 November 2022 (2022-11-09), pages 6786, XP093081024, DOI: 10.1038/s41467-022-33964-9
Attorney, Agent or Firm:
CCPIT PATENT AND TRADEMARK LAW OFFICE (CN)
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