Title:
THERMOCOCCUS ONNURINEUS WTF-156T HAVING MUTATION IN FORMATE TRANSPORTER AND METHODS OF HYDROGEN PRODUCTION USING THEREOF
Document Type and Number:
WIPO Patent Application WO/2018/105790
Kind Code:
A1
Abstract:
The present invention provides Thermococcus onnurineus WTF-156T strain (accession no. KCTC13132BP) having increased hydrogen production ability, wherein there is the mutation at the position 52 of formate transporter from alanine to threonine. In addition, the present invention provides a method for producing hydrogen by using the strain. Thermococcus onnurineus WTF-156T strain according to the present invention shows greater hydrogen production ability form formate than wild-type strain. The method for hydrogen production according to the present invention can produce hydrogen efficiently with lower cost.
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Inventors:
KANG SUNG GYUN (KR)
LEE JUNG-HYUN (KR)
LEE HYUN SOOK (KR)
LEE SEONG HYUK (KR)
JUNG HAE CHANG (KR)
KWON KAE KYOUNG (KR)
LIM JAE KYU (KR)
KIM TAE WAN (KR)
LEE JUNG-HYUN (KR)
LEE HYUN SOOK (KR)
LEE SEONG HYUK (KR)
JUNG HAE CHANG (KR)
KWON KAE KYOUNG (KR)
LIM JAE KYU (KR)
KIM TAE WAN (KR)
Application Number:
PCT/KR2016/014461
Publication Date:
June 14, 2018
Filing Date:
December 09, 2016
Export Citation:
Assignee:
KOREA INST OCEAN SCI & TECH (KR)
International Classes:
C12N1/20; C01B3/02; C12P3/00; C12R1/01
Foreign References:
| KR20110069744A | 2011-06-23 | |||
| KR20150125631A | 2015-11-09 |
Other References:
KIM, YUN JAE ET AL.: "Formate-driven growth coupled with H2 production", NATURE, vol. 467, 16 September 2010 (2010-09-16), pages 352 - 355, XP055513572
BAE, SEUNG SEOB ET AL.: "Enhancing bio-hydrogen production from sodium formate by hyperthermophilic archaeon, Thermococcus onnurineus NA1", BIOPROCESS AND BIOSYSTEMS ENGINEERING, vol. 38, no. 5, 24 December 2014 (2014-12-24), pages 989 - 993, XP055513575
LIPSCOMB, GINA L. ET AL.: "Engineering hydrogen gas production from formate in a hyperthermophile by heterologous production of an 18-subunit membrane- bound complex", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 289, no. 5, 31 January 2014 (2014-01-31), pages 2873 - 2879, XP055513577
DATABASE Protein [O] 31 January 2014 (2014-01-31), NCBI: "Hypothetical formate transporter [Thermococcus onnurineus NA1]", XP055513581, Database accession no. ACJ17063.1
BAE, SEUNG SEOB ET AL.: "Enhancing bio-hydrogen production from sodium formate by hyperthermophilic archaeon, Thermococcus onnurineus NA1", BIOPROCESS AND BIOSYSTEMS ENGINEERING, vol. 38, no. 5, 24 December 2014 (2014-12-24), pages 989 - 993, XP055513575
LIPSCOMB, GINA L. ET AL.: "Engineering hydrogen gas production from formate in a hyperthermophile by heterologous production of an 18-subunit membrane- bound complex", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 289, no. 5, 31 January 2014 (2014-01-31), pages 2873 - 2879, XP055513577
DATABASE Protein [O] 31 January 2014 (2014-01-31), NCBI: "Hypothetical formate transporter [Thermococcus onnurineus NA1]", XP055513581, Database accession no. ACJ17063.1
Attorney, Agent or Firm:
YOON, yuhgang (KR)
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