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Title:
THERMOSTABLE IN VITRO COMPLEX WITH POLYMERASE ACTIVITY
Document Type and Number:
WIPO Patent Application WO2000008164
Kind Code:
A3
Abstract:
Der erfindungsgemässe thermostabile in vitro-Komplex zur Template-abhängigen Elongation von Nukleinsäuren umfasst ein thermostabiles Gleitklammerprotein und ein thermostabiles Elongationsprotein.

Inventors:
KILGER CHRISTIAN (DE)
KOBER INGO (DE)
VOSS HARTMUT (DE)
MOECKEL GERD (DE)
Application Number:
PCT/DE1999/002480
Publication Date:
May 11, 2000
Filing Date:
August 06, 1999
Export Citation:
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Assignee:
LION BIOSCIENCE AG (DE)
KILGER CHRISTIAN (DE)
KOBER INGO (DE)
VOSS HARTMUT (DE)
MOECKEL GERD (DE)
International Classes:
C12N15/09; C07K14/195; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/12; C12N15/31; C12P19/34; C12P21/02; C12Q1/68; C12R1/01; C12R1/19; (IPC1-7): C12N15/31; C07K14/195; C12N9/12; C12P19/34; C12Q1/68
Domestic Patent References:
WO1999013060A11999-03-18
WO1998045452A21998-10-15
WO1999053074A11999-10-21
WO1993015115A11993-08-05
Foreign References:
US5583026A1996-12-10
US5633159A1997-05-27
Other References:
Z. KELMAN AND M. O'DONNEL: "Structural and functional similarities of procaryotic and eucaryotic DNA polymerase sliding clamps", NUCLEIC ACIDS RESEARCH, vol. 23, no. 18, 1995, IRL PRESS LIMITED,OXFORD,ENGLAND, pages 3613 - 3620, XP002128922
YURIEVA O ET AL: "Thermus thermophilis dnaX homolog encoding gamma- and tau-like proteins of the chromosomal replicase", JOURNAL OF BIOLOGICAL CHEMISTRY. (MICROFILMS),US,AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, XP002075006
MCHENRY C S ET AL: "A DNA polymerase III holoenzyme-like subassembly from an extreme thermophilic eubacterium", JOURNAL OF MOLECULAR BIOLOGY,GB,LONDON, XP002075007, ISSN: 0022-2836
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