Title:
THREE-DIMENSIONAL CELL SHAPE EVALUATION METHOD
Document Type and Number:
WIPO Patent Application WO/2020/261607
Kind Code:
A1
Abstract:
A three-dimensional cell shape evaluation method according to an aspect of the present invention comprises: a measurement step (S1) for irradiating, with laser light, one or a plurality of target cells to be evaluated, and detecting interference light between light passing through the target cells and light passing around the target cells to acquire hologram data; a phase image creation step (S2) for performing an image reconstruction process based on the hologram data to create a phase image of the target cells; a differential image creation step (S5) for obtaining a spatial differential value for each pixel from the phase image and creating a differential image; and an evaluation step (S7 to S9) for evaluating the shapes of the target cells, on the basis of a calculation processing result for the differential value of each pixel in the differential image. This makes it possible to easily evaluate the local three-dimensional shape of a cell.
Inventors:
YAMAMOTO SHUHEI (JP)
SAWADA RYUJI (JP)
SAWADA RYUJI (JP)
Application Number:
PCT/JP2019/049553
Publication Date:
December 30, 2020
Filing Date:
December 18, 2019
Export Citation:
Assignee:
SHIMADZU CORP (JP)
International Classes:
C12Q1/02; C12M1/34
Domestic Patent References:
| WO2018158901A1 | 2018-09-07 | |||
| WO2018143406A1 | 2018-08-09 | |||
| WO2018158947A1 | 2018-09-07 | |||
| WO2019043953A1 | 2019-03-07 |
Foreign References:
| JP2014018184A | 2014-02-03 | |||
| JP2010276585A | 2010-12-09 | |||
| JP2019058073A | 2019-04-18 | |||
| JP2013066702A | 2013-04-18 |
Attorney, Agent or Firm:
KYOTO INTERNATIONAL PATENT LAW OFFICE (JP)
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