Title:
THREE-DIMENSIONAL DIFFRACTION TOMOGRAPHY MICROSCOPIC IMAGING METHOD BASED ON LED ARRAY CODING ILLUMINATION
Document Type and Number:
WIPO Patent Application WO/2020/087966
Kind Code:
A1
Abstract:
Disclosed is a three-dimensional diffraction tomography microscopic imaging method based on LED array coding illumination. The method comprises: firstly acquiring an original intensity image; acquiring three groups of intensity image stacks in different defocusing positions by means of moving an objective table or using an electrically controlled zoom lens; then, deducing a three-dimensional phase transfer function of a microscopic imaging system in any shape illumination by means of acquiring intensity image stacks of an object to be tested in the different defocusing positions to obtain a three-dimensional phase transfer function of the microscopic system in circular and annular illumination at different correlation coefficients, and performing deconvolution reconstruction on a three-dimensional diffraction tomography quantitative refractive index; performing an inverse Fourier transform on a three-dimensional scattering potential function; and converting the scattering potential function into refractive index distribution so that the quantitative three-dimensional refractive index distribution of the tested object can be obtained. The high resolution and high signal-to-noise ratio three-dimensional diffraction tomography microscopic imaging of samples, such as cells and tiny biological tissues, is realized.
Inventors:
ZUO CHAO (CN)
CHEN QIAN (CN)
SUN JIASONG (CN)
ZHANG YUZHEN (CN)
GU GUOHUA (CN)
CHEN QIAN (CN)
SUN JIASONG (CN)
ZHANG YUZHEN (CN)
GU GUOHUA (CN)
Application Number:
PCT/CN2019/094886
Publication Date:
May 07, 2020
Filing Date:
July 05, 2019
Export Citation:
Assignee:
UNIV NANJING SCI & TECH (CN)
International Classes:
G01N15/14
Foreign References:
| CN109580457A | 2019-04-05 | |||
| CN108169173A | 2018-06-15 | |||
| CN107290846A | 2017-10-24 | |||
| CN108362643A | 2018-08-03 | |||
| US20100135547A1 | 2010-06-03 |
Other References:
LI, JIAJI ET AL.: "Efficient Quantitative Phase Microscopy Using Programmable Annular LED Illumination", BIOMEDICAL OPTICS EXPRESS, vol. 8, no. 10, 1 October 2017 (2017-10-01), XP055485413, DOI: 20190906155508A
Attorney, Agent or Firm:
JIANGSU SHENGDIAN LAW FIRM (CN)
Download PDF: