Title:
TIRF SINGLE MOLECULE ANALYSIS AND METHOD OF SEQUENCING NUCLEIC ACIDS
Document Type and Number:
WIPO Patent Application WO2006055521
Kind Code:
A9
Abstract:
An apparatus for analyzing the presence of a single molecule comprises a sample plate (30') having a sample located thereon. In one embodiment the apparatus comprises two lasers (46', 46'') for providing illumination light at two different wavelengths suitable for exciting fluorescence in the sample, a collimator comprising a band-pass filter (50', 55''), a diverging lens (54', 54''), a field stop (62'), and a converging lens (66') for directing the illunination light through a total internal reflection objective (74') onto the sample, and means (34', 34'') for detecting a fluorescent image produced by said sample in response to the illunination light. In one embodiment the apparatus further comprises an auto-focus module comprising a laser (38'), a beam splitter cube (86'), a converging lens (90'), and an auto-focus dichroec (94') for maintaining focus of the objective on the sample.
Inventors:
HARRIS TIMOTHY D (US)
BUZBY PHILIP R (US)
JAROSZ MIRNA (US)
GILL JAIME (US)
WEISS HOWARD (US)
LAPIDUS STANLEY N (US)
BUZBY PHILIP R (US)
JAROSZ MIRNA (US)
GILL JAIME (US)
WEISS HOWARD (US)
LAPIDUS STANLEY N (US)
Application Number:
PCT/US2005/041264
Publication Date:
August 17, 2006
Filing Date:
November 15, 2005
Export Citation:
Assignee:
HELICOS BIOSCIENCES CORP (US)
HARRIS TIMOTHY D (US)
BUZBY PHILIP R (US)
JAROSZ MIRNA (US)
GILL JAIME (US)
WEISS HOWARD (US)
LAPIDUS STANLEY N (US)
HARRIS TIMOTHY D (US)
BUZBY PHILIP R (US)
JAROSZ MIRNA (US)
GILL JAIME (US)
WEISS HOWARD (US)
LAPIDUS STANLEY N (US)
International Classes:
G01N21/64; C12Q1/68
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