Title:
TISSUE ENGINEERING FOR MAKING TUBULAR IMPLANTS
Document Type and Number:
WIPO Patent Application WO/2000/067672
Kind Code:
A2
Abstract:
A method for making a tubular structure for surgical implantation into a donor host, characterised in that it comprises: taking a sample of appropriate tissue from the donor host; extracting substantially all cells therefrom; growing the cells in tissue culture; innoculating a generally planar scaffold with the cultured cels; subjecting the innoculated scaffold to further tissue culture; removing the scaffold from tissue culture once the innoculated cells are established; cutting the neo-tissue to a desired profile for the intended use; joining and sealing the edges of the generally planar neo-tissue to form a tubular structure; and subjecting the tubular structure to physiological load conditions until the neo-tissue has substantially similar histological appearance and mechanical properties to the naturally-occurring counterpart is disclosed.
Inventors:
EASTWOOD MARK (GB)
Application Number:
PCT/EP2000/004412
Publication Date:
November 16, 2000
Filing Date:
May 04, 2000
Export Citation:
Assignee:
UNIV WESTMINSTER (GB)
EASTWOOD MARK (GB)
EASTWOOD MARK (GB)
International Classes:
A61F2/06; A61L27/38; A61L27/50; C12N5/00; C12N5/071; (IPC1-7): A61F2/04; A61F2/24; C12N5/00; A61L27/38
Domestic Patent References:
| WO1996021003A1 | 1996-07-11 |
Foreign References:
| US5863531A | 1999-01-26 | |||
| US5855610A | 1999-01-05 |
Attorney, Agent or Firm:
Froud, Clive (Elkington and Fife Prospect House 8 Pembroke
Road Sevenoaks Kent TN13 1XR, GB)
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Claims:
Claims:
| 1. | A method for making a tubular structure for surgical implantation into a donor host, characterised in that it comprises: taking a sample of appropriate tissue from the donor host; extracting substantially all cells therefrom; growing the cells in tissue culture; innoculating a generally planar scaffold with the cultured cells; subjecting the innoculated scaffold to further tissue culture; removing the scaffold from tissue culture once the innoculated cells are established; cutting the neo tissue to a desired profile for the intended use; joining and sealing the edges of the generally planar neotissue to form a tubular structure; and subjecting the tubular structure to physiological load conditions until the neotissue has substantially similar histological appearance and mechanical properties to the naturally occurring counterpart. |
| 2. | A method for making a tubular tissue structure which is a blood vessel or a heart valve or a part thereof for surgical implantation into a donor host, characterised in that it comprises: taking a sample of appropriate tissue from the donor host; extracting substantially all cells therefrom, starting with endothelial cells; growing the endothelial cells and the other cells in separate tissue cultures; innoculating a generally planar scaffold with the cultured nonendothelial cells; subjecting the innoculated scaffold to further tissue culture; removing the scaffold from tissue culture once the innoculated cells are established; applying a layer of the cultured endothelial cells to at least one side of the scaffold; subjecting the scaffold to further tissue culture; removing the resulting neotissue from tissue culture once the endothelial cells are established on at least one side; cutting the neotissue to a desired profile for the intended use; joining and sealing the edges of the generally planar neotissue to form a tubular structure having endothelial cells at least on the inside; and subjecting the tubular structure to physiological load conditions until the neotissue has substantially similar histological appearance and mechanical properties to the naturallyoccurring counterpart. |
| 3. | A method as claimed in claim 2 wherein the tubular structure has internal features resulting from the form of the cut profile. |
| 4. | A method as claimed in claim 2 or claim 3 wherein the tubular structure has internal features resulting from the addition of endothelial cellcoated appendages to an endothelial cellcoated side of the neotissue prior to formation of the tubular structure. |
| 5. | A method as claimed in any of claims 2 to 4 wherein a sample of vascular material, such as saphenous vein or artery, is taken. |
| 6. | A method as claimed in any of claims 1 to 5 wherein a TCM apparatus is used to provide physiological load conditions. |
| 7. | A method as claimed in any of claims 2 to 6 wherein a pulse duplicator and/or a mock circulation system is used to provide physiological load conditions. |
| 8. | A method as claimed in any of claims 1 to 7 wherein at least one suture and/or fibrin sealant is used to join and seal the edges when forming the tubular structure. |
Description:
INTERNATIONALSEARCHREPORT Internial ApplicationNo i,.,ormationonpatentfamily members PCT/EP00/04412 PatentdocumentPublication PatentPublication citedinsearchreportdate member(s)date WO9621003A11-07-1996US 5618718 A 08-04-1997 AU 695407 B 13-08-1998 AU 4295496 A 24-07-1996 CA 2209211 A 11-07-1996 EP 0801677 A 22-10-1997 JP 10511847 T 17-11-1998 NZ 297719 A 25-11-1998 US5863531A26-01-1999US 5443950 A 22-08-1995 US 5266480 A 30-11-1993 US 5032508 A 16-07-1991 US 4963489 A 16-10-1990 US 4721096 A 26-01-1988 AU 706426 B 17-06-1999 AU 6031596 A 30-12-1996 CA 2224071 A 19-12-1996 EP 0831861 A 01-04-1998 JP 11506611 T 15-06-1999 NZ 310004 A 28-10-1999 WO 9640175 A 19-12-1996 US 6140039 A 31-10-2000 US 6022743 A 08-02-2000 AU 689605 B 02-04-1998 AU 2769695 A 04-01-1996 CA 2192064 A 14-12-1995 EP 0812351 A 17-12-1997 NZ 288467 A 28-10-1998 WO 9533821 A 14-12-1995 US 5962325 A 05-10-1999 US 5902741 A 11-05-1999 US 5460939 A 24-10-1995 US 5510254 A 23-04-1996 US 5580781 A 03-12-1996 US 5516680 A 14-05-1996 US 5512475 A 30-04-1996 US 5541107 A 30-07-1996 US 5516681 A 14-05-1996 US 5578485 A 26-11-1996 US 5785964 A 28-07-1998 US 5518915 A 21-05-1996 US 5624840 A 29-04-1997 US 5849588 A 15-12-1998 US 5858721 A 12-01-1999 AU 644578 B 16-12-1993 AU 4211489 A 02-04-1990 BR 8907642 A 20-08-1991 CA 1335657 A 23-05-1995 DK 40591 A 07-05-1991 EP 0358506 A 14-03-1990 HU 56393 A 28-08-1991 IL 91536 A 31-10-1996 JP 4501657 T 26-03-1992 KR 156571 B 15-10-1998 KR 156684 B 15-10-1998 KR 156685 B 15-10-1998 NO 910787 A 22-04-1991 NZ 230572 A 23-12-1993 PT 91676 A 30-03-1990 INTERNATIONALSEARCHREPORT InternalApplicationNo m,ormation on patent familymembersPCT/E P 00/04412 PatentdocumentPublicationPatentfamily Publication citedinsearchreportdatemember(s)date US 5855610 A 05-01-1999 NONE
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