Title:
TOPICAL COMPOSITIONS AND USES
Document Type and Number:
WIPO Patent Application WO/2019/015673
Kind Code:
A1
Abstract:
Provided are chimeric polypeptides that include one or more zinc finger motifs fused to a therapeutic peptide such as botulinum neurotoxins (BoNTs). The zinc finger motif may be located at the C-terminal side of the BoNT and the chimeric polypeptide can optionally include two or more such zinc finger motifs. It said the disclosed chimeric polypeptides can be efficiently delivered to a subject transdermally.
Inventors:
LIU JIA (CN)
JIANG BIAO (CN)
JIANG BIAO (CN)
Application Number:
PCT/CN2018/096448
Publication Date:
January 24, 2019
Filing Date:
July 20, 2018
Export Citation:
Assignee:
UNIV SHANGHAI TECH (CN)
International Classes:
A61K51/00; A61K39/40
Domestic Patent References:
| WO2005084410A2 | 2005-09-15 |
Other References:
KIM, D.W. ET AL.: "Expression, Purification and Transduction of PEP-1-Botulinum Neurotoxin Type A (PEP-1-BoNT/A) into Skin", JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, vol. 39, no. 5, 30 September 2006 (2006-09-30), pages 642 - 647, XP055076669
GAJ, T. ET AL.: "Direct Protein Delivery to Mammalian Cells Using Cell -permeable Cys2-His2 Zinc-finger Domains", JOURNAL OF VISUALIZED EXPERIMENTS, vol. 97, 25 March 2015 (2015-03-25), pages 1 - 7, XP055565466
AMANI, J. ET AL.: "Designing and analyzing the structure of Tat-BoNT/A(l-448)fusion protein: An in silico approach", MOLECULAR BIOLOGY RESEARCH COMMUNICATIONS, vol. 3, no. 2, 31 December 2014 (2014-12-31), pages 115 - 127, XP055565470
GAJ, T. ET AL.: "Protein Delivery Using Cys2-His2 Zinc-Finger Domains", ACS CHEMICAL BIOLOGY, vol. 9, 17 June 2014 (2014-06-17), pages 1662 - 1667, XP055499064
PICKETT, A. ET AL.: "Towards New Uses of Botulinum Toxin as a Novel Therapeutic Tool", TOXINS, vol. 3, 12 January 2011 (2011-01-12), pages 63 - 81, XP055565473
See also references of EP 3655043A4
GAJ, T. ET AL.: "Direct Protein Delivery to Mammalian Cells Using Cell -permeable Cys2-His2 Zinc-finger Domains", JOURNAL OF VISUALIZED EXPERIMENTS, vol. 97, 25 March 2015 (2015-03-25), pages 1 - 7, XP055565466
AMANI, J. ET AL.: "Designing and analyzing the structure of Tat-BoNT/A(l-448)fusion protein: An in silico approach", MOLECULAR BIOLOGY RESEARCH COMMUNICATIONS, vol. 3, no. 2, 31 December 2014 (2014-12-31), pages 115 - 127, XP055565470
GAJ, T. ET AL.: "Protein Delivery Using Cys2-His2 Zinc-Finger Domains", ACS CHEMICAL BIOLOGY, vol. 9, 17 June 2014 (2014-06-17), pages 1662 - 1667, XP055499064
PICKETT, A. ET AL.: "Towards New Uses of Botulinum Toxin as a Novel Therapeutic Tool", TOXINS, vol. 3, 12 January 2011 (2011-01-12), pages 63 - 81, XP055565473
See also references of EP 3655043A4
Attorney, Agent or Firm:
SHANGHAI BESHINING LAW OFFICE (CN)
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