TOTAL REFLECTION FLUORESCENCE OBSERVATION DEVICE

WIPO Patent Application WO/2010/010751

A1

A technique and device for fluorescence observation with good operability, high sensitivity, and high reliability. The device is used for fluorescence observation using evanescent light.  The angle of incidence of the exciting light is adjusted so that the exciting light is always totally reflected from the surface of a substrate irrespective of the angle of the surface of the substrate.  The method includes a step of shining the exciting light on the observation substrate while continuously varying the angle of the exciting light with respect to the observation substrate, a step of sensing the shone exciting light by means of optical sensors, and a step of setting the angle of total reflection according to the result of the sensing by the optical sensors.  The direction in which the shone exciting light travels varies with the angle of incidence.  That is, the exciting light travels as the transmitted light, the reflected light, or the surface propagating light.  These lights are sensed by the corresponding optical sensors, and how the angle of incidence of the exciting light is with respect to the critical angle is determined.  The angle of incidence of the exciting light is varied depending on the result of the determination, thereby realizing an optimum total reflection angle.

KUMAZAKI NOBUTAKA (JP)
TAKAHASHI SATOSHI (JP)
KATO HIROKAZU (JP)
HAGA TAKANOBU (JP)

PCT/JP2009/059635

January 28, 2010

May 20, 2009

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HITACHI HIGH TECH CORP (JP)
KUMAZAKI NOBUTAKA (JP)
TAKAHASHI SATOSHI (JP)
KATO HIROKAZU (JP)
HAGA TAKANOBU (JP)

G02B21/16; G01N21/64

JP2006201465A	2006-08-03
JP2006189741A	2006-07-20
JPH03246452A	1991-11-01

INOUE, Manabu (ITD. 6-1, Marunouchi 1-chome,Chiyoda-k, TOKYO 20, JP)

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