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Title:
TRANSAMINASE MUTANT AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2019/148494
Kind Code:
A1
Abstract:
Provided are a transaminase mutant and an application thereof. The amino acid sequence of the transaminase mutant is formed after mutation of the amino acid sequence as shown in SEQ ID NO: 1, and mutated amino acid sites comprise a T7C+S47C site. The transaminase mutant having the mutated sites can be further prepared into an immobilized enzyme by means of immobilization technology, the activity and the stability of the immobilized enzyme are relatively high, and the immobilized enzyme can be recycled for a plurality of times, and is applicable to continuous flow reaction in a packed bed.

Inventors:
HONG HAO (CN)
JAMES GAGE (CN)
LU JIANGPING (CN)
XU XINGFU (CN)
CUI YUXIA (CN)
ZHANG NA (CN)
DONG XUEWU (CN)
YU WENYAN (CN)
HUANG XIN (CN)
HAO MINGMIN (CN)
MA YULEI (CN)
CHENG YIBING (CN)
ZHAO JIADONG (CN)
Application Number:
PCT/CN2018/075272
Publication Date:
August 08, 2019
Filing Date:
February 05, 2018
Export Citation:
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Assignee:
ASYMCHEM LIFE SCIENCE TIANJIN CO LTD (CN)
International Classes:
C12N9/10; C07K14/195; C12N15/54; C12N15/63; C12P41/00
Foreign References:
US20160298092A12016-10-13
CN104894148A2015-09-09
CN106676142A2017-05-17
CN102341494A2012-02-01
Other References:
KARIM ENGELMARK CASSIMJEE: "Chromobacterium violaceum omega -transaminase variant Trp60Cys shows increased specificity for (S)-1-phenylethylamine and 4' -substituted acetophenones, and follows Swain - Lupton parameterisation", ORGANIC & BIOMOLECULAR CHEMISTRY, 31 December 2012 (2012-12-31), XP055345916, ISSN: 1477-0520, doi:10.1039/c2ob25893e
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See also references of EP 3733689A4
Attorney, Agent or Firm:
KANGXIN PARTNERS, P.C. (CN)
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