Title:
TRANSAMINASE MUTANT AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2022/166103
Kind Code:
A1
Abstract:
Provided are a transaminase mutant and an application thereof. The transaminase mutant is obtained by mutating one or more amino acids in SEQ ID NO: 2 or is a mutant obtained by taking the sequence SEQ ID NO: 1 of wild-type CVTA transaminase as a reference and mutating conserved amino acids therein. Compared with wild-type transaminases, the catalytic activity of the mutant is improved to different degrees, so that the production efficiency of chiral amine compound synthesis can be improved.
Inventors:
HONG HAO (US)
JAMES GAGE (US)
XIAO YI (CN)
ZHANG NA (CN)
JIAO XUECHENG (CN)
MA YULEI (CN)
MOU HUIYAN (CN)
WANG ZUJIAN (CN)
SUN KAIHUA (CN)
LI XIANG (CN)
ZHAO TONG (CN)
CAO SHAN (CN)
JAMES GAGE (US)
XIAO YI (CN)
ZHANG NA (CN)
JIAO XUECHENG (CN)
MA YULEI (CN)
MOU HUIYAN (CN)
WANG ZUJIAN (CN)
SUN KAIHUA (CN)
LI XIANG (CN)
ZHAO TONG (CN)
CAO SHAN (CN)
Application Number:
PCT/CN2021/104841
Publication Date:
August 11, 2022
Filing Date:
July 06, 2021
Export Citation:
Assignee:
ASYMCHEM LAB TIANJIN CO LTD (CN)
International Classes:
C12N9/10; C12N15/54; C12P13/00; C12P17/12; C12P17/14
Foreign References:
| CN112980810A | 2021-06-18 | |||
| CN112501145A | 2021-03-16 | |||
| CN111032682A | 2020-04-17 | |||
| CN110592042A | 2019-12-20 | |||
| CN107828751A | 2018-03-23 | |||
| CN102597226A | 2012-07-18 | |||
| CN109957554A | 2019-07-02 |
Other References:
DATABASE Protein ANONYMOUS : "aspartate aminotransferase family protein [Chromobacterium violaceum]", XP055956471, retrieved from Genbank
HECKMANN CHRISTIAN M., GOURLAY LOUISE J., DOMINGUEZ BEATRIZ, PARADISI FRANCESCA: "An (R)-Selective Transaminase From Thermomyces stellatus: Stabilizing the Tetrameric Form", FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, vol. 8, XP055957367, DOI: 10.3389/fbioe.2020.00707
ANGELW. CHEM. INT. ED., vol. 46, 2007, pages 4367
SCIENCE, vol. 329, 2010, pages 305
HECKMANN CHRISTIAN M., GOURLAY LOUISE J., DOMINGUEZ BEATRIZ, PARADISI FRANCESCA: "An (R)-Selective Transaminase From Thermomyces stellatus: Stabilizing the Tetrameric Form", FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, vol. 8, XP055957367, DOI: 10.3389/fbioe.2020.00707
ANGELW. CHEM. INT. ED., vol. 46, 2007, pages 4367
SCIENCE, vol. 329, 2010, pages 305
Attorney, Agent or Firm:
KANGXIN PARTNERS, P.C. (CN)
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