Title:
TRANSAMINASE MUTANT AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2021/217773
Kind Code:
A1
Abstract:
Provided in the present invention are a transaminase mutant and use thereof. Compared with an amino acid sequence shown in SEQ ID NO: 1, an amino acid sequence of the transaminase mutant comprises at least one of the following mutation sites: L166, K149, K146, A168, H73, F133, H82, E24, V194, T294, A295, G235 and F236. The mutant of the present invention has an improved catalytic activity for a transammonization reaction of ketone substrates, and is suitable for industrial production of chiral amines.
Inventors:
HONG HAO (US)
JAMES GAGE (US)
XIAO YI (CN)
ZHANG NA (CN)
JIAO XUECHENG (CN)
MA YULEI (CN)
MOU HUIYAN (CN)
CAO SHAN (CN)
JAMES GAGE (US)
XIAO YI (CN)
ZHANG NA (CN)
JIAO XUECHENG (CN)
MA YULEI (CN)
MOU HUIYAN (CN)
CAO SHAN (CN)
Application Number:
PCT/CN2020/093528
Publication Date:
November 04, 2021
Filing Date:
May 29, 2020
Export Citation:
Assignee:
ASYMCHEM LAB TIANJIN CO LTD (CN)
International Classes:
C12N9/10; C12N1/21; C12N15/54; C12N15/70; C12P13/00
Foreign References:
| CN107828751A | 2018-03-23 | |||
| CN107805648A | 2018-03-16 | |||
| CN108823179A | 2018-11-16 | |||
| CN107828751A | 2018-03-23 |
Other References:
TANG XIAO-LING; ZHANG NAN-NAN; YE GUO-YAN; ZHENG YU-GUO: "Efficient biosynthesis of (R)-3-amino-1-butanol by a novel (R)-selective transaminase fromActinobacteriasp.", JOURNAL OF BIOTECHNOLOGY, ELSEVIER, AMSTERDAM NL, vol. 295, 1 January 1900 (1900-01-01), Amsterdam NL , pages 49 - 54, XP085641894, ISSN: 0168-1656, DOI: 10.1016/j.jbiotec.2019.02.008
Attorney, Agent or Firm:
KANGXIN PARTNERS, P. C. (CN)
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