Title:
TRANSAMINASE MUTANT AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/201809
Kind Code:
A1
Abstract:
Provided are a transaminase mutant and use thereof, wherein the transaminase mutant is obtained by mutation of an amino acid sequence set forth in SEQ ID NO: 1, the mutation at least comprising: T291A; or the amino acid sequence of the transaminase mutant has the mutation site in the mutated amino acid sequence, and has 80% or more homology, preferably 85% homology, and more preferably 90% homology to the mutated amino acid sequence. The transaminase mutant expands the substrate spectrum of the enzyme and improves the stereoselectivity, such that the catalytic capability of the enzyme to a large-steric-hindrance substrate is improved. Meanwhile, the stability of the enzyme is enhanced, such that a catalytic reaction can be carried out under the conditions of a high-concentration solvent and a high temperature to produce chiral amine.
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Inventors:
HONG HAO (US)
JAMES GAGE (US)
XIAO YI (CN)
ZHANG NA (CN)
JIAO XUECHENG (CN)
ZHANG KEJIAN (CN)
WANG LEI (CN)
ZHAO JUNQI (CN)
LING JUN (CN)
XU XINSHENG (CN)
LI RUI (CN)
WANG ZUJIAN (CN)
JAMES GAGE (US)
XIAO YI (CN)
ZHANG NA (CN)
JIAO XUECHENG (CN)
ZHANG KEJIAN (CN)
WANG LEI (CN)
ZHAO JUNQI (CN)
LING JUN (CN)
XU XINSHENG (CN)
LI RUI (CN)
WANG ZUJIAN (CN)
Application Number:
PCT/CN2022/093313
Publication Date:
October 26, 2023
Filing Date:
May 17, 2022
Export Citation:
Assignee:
ASYMCHEM LAB TIANJIN CO LTD (CN)
International Classes:
C12N9/10; C12N1/19; C12N1/21; C12N15/54; C12N15/70; C12N15/81; C12P13/00; C12R1/19; C12R1/645
Foreign References:
| US20210317421A1 | 2021-10-14 | |||
| CN110628742A | 2019-12-31 | |||
| CN111032682A | 2020-04-17 |
Other References:
DATABASE PROTEIN ANONYMOUS : "aminotransferase class IV [Sciscionella sp. SE31]", XP093103502, retrieved from NCBI
WANG CHEN, TANG KEXIN, DAI YA, JIA HONGHUA, LI YAN, GAO ZHEN, WU BIN: "Identification, Characterization, and Site-Specific Mutagenesis of a Thermostable ω-Transaminase from Chloroflexi bacterium", ACS OMEGA, ACS PUBLICATIONS, US, vol. 6, no. 26, 6 July 2021 (2021-07-06), US , pages 17058 - 17070, XP093103505, ISSN: 2470-1343, DOI: 10.1021/acsomega.1c02164
XU WEI, LI YAN, YAN MING: "The Applications of Site-directed Mutagenesis in Aminotransferase", JOURNAL OF YANCHENG INSTITUTE OF TECHNOLOGY (NATURAL SCIENCE EDITION), vol. 17, no. 4, 31 December 2004 (2004-12-31), pages 55 - 58, XP093103510, ISSN: 1671-5322, DOI: 10.16018/j.cnki.32-1650/n.2004.04.015
WANG CHEN, TANG KEXIN, DAI YA, JIA HONGHUA, LI YAN, GAO ZHEN, WU BIN: "Identification, Characterization, and Site-Specific Mutagenesis of a Thermostable ω-Transaminase from Chloroflexi bacterium", ACS OMEGA, ACS PUBLICATIONS, US, vol. 6, no. 26, 6 July 2021 (2021-07-06), US , pages 17058 - 17070, XP093103505, ISSN: 2470-1343, DOI: 10.1021/acsomega.1c02164
XU WEI, LI YAN, YAN MING: "The Applications of Site-directed Mutagenesis in Aminotransferase", JOURNAL OF YANCHENG INSTITUTE OF TECHNOLOGY (NATURAL SCIENCE EDITION), vol. 17, no. 4, 31 December 2004 (2004-12-31), pages 55 - 58, XP093103510, ISSN: 1671-5322, DOI: 10.16018/j.cnki.32-1650/n.2004.04.015
Attorney, Agent or Firm:
KANGXIN PARTNERS, P.C. (CN)
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