Title:
TRANSCRIPTIONAL REGULATORY ELEMENT AND ITS USE IN ENHANCING THE EXPRESSION OF EXOGENOUS PROTEIN
Document Type and Number:
WIPO Patent Application WO/2020/034097
Kind Code:
A1
Abstract:
Provided a polynucleotide which can be used as a WXRE transcriptional regulatory element to increase the protein expression level of a protein expression system, and a protein expression vector or a protein expression system comprising the WXRE transcriptional regulatory element as well as the uses thereof. The use of the transcriptional regulatory element WXRE can increase the expression level of an exogenous protein greatly with biological activity unchanged.
Inventors:
ZHANG ZHENG (CN)
LI XIAOLU (CN)
ZHANG YUCHEN (CN)
LI YARONG (CN)
DONG HUIFANG (CN)
CAI JILL (CN)
ZHOU WEICHANG (CN)
CHEN CHRIS (CN)
LI XIAOLU (CN)
ZHANG YUCHEN (CN)
LI YARONG (CN)
DONG HUIFANG (CN)
CAI JILL (CN)
ZHOU WEICHANG (CN)
CHEN CHRIS (CN)
Application Number:
PCT/CN2018/100467
Publication Date:
February 20, 2020
Filing Date:
August 14, 2018
Export Citation:
Assignee:
WUXI BIOLOGICS SHANGHAI CO LTD (CN)
International Classes:
C12N15/113; A61P35/00; A61P37/00; C12N5/10; C12N15/85; C12P21/08
Foreign References:
| CN105255895A | 2016-01-20 | |||
| CN103834686A | 2014-06-04 | |||
| CN106432501A | 2017-02-22 |
Other References:
LI, W.M. ET AL.: "Research Advance in Genome-wide Identification of Transcription Regulatory Elements", BIOTECHONOLOGYBULLETIN, 31 October 2014 (2014-10-31), pages 64 - 70, ISSN: 1002-5464
YANG, J.X. ET AL.: "Sterol-resistant transcription in CHO cells caused by gene rearrangement that truncates SREBP-2", GENES & DEVELOPMENT, vol. 8, 15 August 1994 (1994-08-15), pages 1910 - 1919, XP000979471, ISSN: 0890-9369
YANG, J.X. ET AL.: "Sterol-resistant transcription in CHO cells caused by gene rearrangement that truncates SREBP-2", GENES & DEVELOPMENT, vol. 8, 15 August 1994 (1994-08-15), pages 1910 - 1919, XP000979471, ISSN: 0890-9369
Attorney, Agent or Firm:
LINDA LIU & PARTNERS (CN)
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