Title:
TRANSFORMANT OF CORYNEFORM BACTERIUM AND METHOD FOR PRODUCING VALINE BY USING SAME
Document Type and Number:
WIPO Patent Application WO/2013/027709
Kind Code:
A1
Abstract:
This transformant is obtained by introducing, to a host coryneform bacterium, at least one of the DNAs (a), (b), and (c): (a) a DNA, or an analog thereof, obtained by introducing, to a DNA that encodes an acetohydroxy acid synthase derived from Corynebacterium glutamicum, a mutation that mutates the No. 156 glycine in the amino acid sequence encoded by this DNA to glutamic acid (G156E); (b) a DNA, or an analog thereof, obtained by introducing, to a DNA that encodes an acetohydroxy acid isomeroreductase derived from Corynebacterium glutamicum, mutations that mutate the No. 34 serine in the amino acid sequence encoded by this DNA to glycine (S34G), mutate the No. 48 leucine to glutamic acid (L48E), and mutate the No. 49 arginine to phenylalanine (R49F); and (c) a DNA, or an analog thereof, that encodes a leucine dehydrogenase derived from Lysinibacillus sphaericus.
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Inventors:
YUKAWA HIDEAKI (JP)
INUI MASAYUKI (JP)
INUI MASAYUKI (JP)
Application Number:
PCT/JP2012/071019
Publication Date:
February 28, 2013
Filing Date:
August 21, 2012
Export Citation:
Assignee:
RES INST INNOVATIVE TECH EARTH (JP)
YUKAWA HIDEAKI (JP)
INUI MASAYUKI (JP)
YUKAWA HIDEAKI (JP)
INUI MASAYUKI (JP)
International Classes:
C12N1/21; C12N15/09; C12P13/08; C12R1/15
Domestic Patent References:
| WO2005010182A1 | 2005-02-03 |
Foreign References:
| JP2002537771A | 2002-11-12 | |||
| JP2007043947A | 2007-02-22 | |||
| JP2009521960A | 2009-06-11 | |||
| JP2008514191A | 2008-05-08 | |||
| JPS5867699A | 1983-04-22 | |||
| JPS57183799A | 1982-11-12 | |||
| JPS62166890A | 1987-07-23 | |||
| US6303383B1 | 2001-10-16 | |||
| JPH057491A | 1993-01-19 | |||
| JPS57134500A | 1982-08-19 |
Other References:
ELISAKOVA, V. ET AL.: "Feedback-Resistant Acetohydroxy Acid Synthase Increases Valine Production in Corynebacterium glutamicum", APPL. ENVIRON. MICROBIOL., vol. 71, no. 1, 2005, pages 207 - 213, XP008129187
HOLATKO, J. ET AL.: "Metabolic engineering of the L-valine biosynthesis pathway in Corynebacterium glutamicum using promoter activity modulation", J. BIOTECHNOL., vol. 139, no. 3, 2009, pages 203 - 210, XP025925417
BLOMBACH, B. ET AL.: "Corynebacterium glutamicum tailored for high-yield L-valine production.", APPL. MICROBIOL. BIOTECHNOL., vol. 79, no. 3, 2008, pages 471 - 479, XP019623597
LEYVAL D. ET AL.: "Characterisation of the enzyme activities involved in the valine biosynthetic pathway in a valine-producing strain of Corynebacterium glutamicum", J. BIOTECHNOL., vol. 104, no. 1-3, 2003, pages 241 - 252, XP002380799
"Bergey's Manual of Determinative Bacteriology", vol. 8, 1974, pages: 599
LIEBL, W. ET AL.: "Transfer of Brevibacterium divaricatum DSM 20297T, ''Brevibacterium flavum'' DSM 20411, ''Brevibacterium lactofermentum'' DSM 20412 and DSM 1412, and Corynebacterium glutamicum and their distinction by rRNA gene restriction patterns", INT. J. SYST. BACTERIOL., vol. 41, 1991, pages 255 - 260, XP002932537
KAZUO KOMAGATA ET AL.: "Classification of the coryneform group of bacteria", FERMENTATION AND INDUSTRY, vol. 45, 1987, pages 944 - 963
"Molecular Cloning, A Laboratory Manual, Second edition,", vol. 2, 1989, pages: 11 - 45
DENNIS H.F.: "The role and properties of the iron-sulfur cluster in Escherichia coli dihydroxy-acid dehydratase", J. BIOL. CHEM., vol. 268, 1993, pages 14732 - 14742
MIWA, K. ET AL.: "Cryptic plasmids in glutamic acid-producing bacteria", AGRIC. BIOL. CHEM., vol. 48, 1984, pages 2901 - 2903, XP001309224
YAMAGUCHI, R. ET AL.: "Determination of the complete nucleotide sequence of the Brevibacterium lactofermentum plasmid pAM330 and the analysis of its genetic information", NUCLEIC ACIDS SYMP. SER., vol. 16, 1985, pages 265 - 267
KURUSU, Y. ET AL.: "Identification of plasmid partition function in coryneform bacteria", APPL. ENVIRON. MICROBIOL., vol. 57, 1991, pages 759 - 764
KATSUMATA, R. ET AL.: "Protoplast transformation of glutamate-producing bacteria with plasmid DNA", J. BACTERIOL., vol. 159, 1984, pages 306 - 311
EIKMANNS, B. J. ET AL.: "A family of Corynebacterium glutamicum/Escherichia coli shuttle vectors for cloning, controlled gene expression, and promoter probing", GENE, vol. 102, 1991, pages 93 - 98, XP023541652, DOI: doi:10.1016/0378-1119(91)90545-M
KURUSU, Y. ET AL.: "Electroporation-transformation system for Coryneform bacteria by auxotrophic complementation.", AGRIC. BIOL. CHEM., vol. 54, 1990, pages 443 - 447
VERTES A.A. ET AL.: "Presence of mrr- and mcr-like restriction systems in Coryneform bacteria", RES. MICROBIOL., vol. 144, 1993, pages 181 - 185, XP023924914, DOI: doi:10.1016/0923-2508(93)90043-2
INUI, M. ET AL.: "Metabolic analysis of Corynebacterium glutamicum during lactate and succinate productions under oxygen deprivation conditions", J. MOL. MICROBIOL. BIOTECHNOL., vol. 7, 2004, pages 182 - 196, XP009070835, DOI: doi:10.1159/000079827
C. A ET AL.: "Corynebacterium glutamicumglyceraldehyde-3-phosphate dehydrogenase isoforms with opposite, ATP-dependent regulation.", J. MOL. MICROBIOL. BIOTECHNOL., vol. 8, 2004, pages 91 - 103
J. MOL. MICROBIOL. BIOTECHNOL., vol. 8, 2004, pages 243 - 254
OMUMASABA, C. A. ET AL.: "Corynebacterium glutamicum glyceraldehyde-3-phosphate dehydrogenase isoforms with opposite, ATP-dependent regulation", J. MOL. MICROBIOL. BIOTECHNOL., vol. 8, 2004, pages 91 - 103
PFENNIG, N. ET AL.: "The Prokaryotes, A Handbook on Habitats, Isolation and Identification of Bacteria", 1981, SPRINGER VERLAG, article "The dissimilatory sulfate-reducing bacteria", pages: 926 - 940
"Nogeikagaku Jikkensho", vol. 3, 1990, SANGYO TOSHO, pages: 26
OMUMASABA, C. A. ET AL.: "Corynebacterium glutamicum glyceraldehyde-3-phosphate dehydrogenase isoforms with opposite, ATP-dependent regulation.", J. MOL. MICROBIOL. BIOTECHNOL., vol. 8, 2004, pages 91 - 103
JOURNAL OF MOLECULAR BIOLOGY, vol. 53, 1970, pages 159
J. MOL. BIOTECHNOL., vol. 8, 2004, pages 243 - 254
AGRIC. BIOL. CHEM., vol. 54, 1990, pages 443 - 447
RES. MICROBIOL., vol. 144, 1993, pages 181 - 185
See also references of EP 2749638A4
HOLATKO, J. ET AL.: "Metabolic engineering of the L-valine biosynthesis pathway in Corynebacterium glutamicum using promoter activity modulation", J. BIOTECHNOL., vol. 139, no. 3, 2009, pages 203 - 210, XP025925417
BLOMBACH, B. ET AL.: "Corynebacterium glutamicum tailored for high-yield L-valine production.", APPL. MICROBIOL. BIOTECHNOL., vol. 79, no. 3, 2008, pages 471 - 479, XP019623597
LEYVAL D. ET AL.: "Characterisation of the enzyme activities involved in the valine biosynthetic pathway in a valine-producing strain of Corynebacterium glutamicum", J. BIOTECHNOL., vol. 104, no. 1-3, 2003, pages 241 - 252, XP002380799
"Bergey's Manual of Determinative Bacteriology", vol. 8, 1974, pages: 599
LIEBL, W. ET AL.: "Transfer of Brevibacterium divaricatum DSM 20297T, ''Brevibacterium flavum'' DSM 20411, ''Brevibacterium lactofermentum'' DSM 20412 and DSM 1412, and Corynebacterium glutamicum and their distinction by rRNA gene restriction patterns", INT. J. SYST. BACTERIOL., vol. 41, 1991, pages 255 - 260, XP002932537
KAZUO KOMAGATA ET AL.: "Classification of the coryneform group of bacteria", FERMENTATION AND INDUSTRY, vol. 45, 1987, pages 944 - 963
"Molecular Cloning, A Laboratory Manual, Second edition,", vol. 2, 1989, pages: 11 - 45
DENNIS H.F.: "The role and properties of the iron-sulfur cluster in Escherichia coli dihydroxy-acid dehydratase", J. BIOL. CHEM., vol. 268, 1993, pages 14732 - 14742
MIWA, K. ET AL.: "Cryptic plasmids in glutamic acid-producing bacteria", AGRIC. BIOL. CHEM., vol. 48, 1984, pages 2901 - 2903, XP001309224
YAMAGUCHI, R. ET AL.: "Determination of the complete nucleotide sequence of the Brevibacterium lactofermentum plasmid pAM330 and the analysis of its genetic information", NUCLEIC ACIDS SYMP. SER., vol. 16, 1985, pages 265 - 267
KURUSU, Y. ET AL.: "Identification of plasmid partition function in coryneform bacteria", APPL. ENVIRON. MICROBIOL., vol. 57, 1991, pages 759 - 764
KATSUMATA, R. ET AL.: "Protoplast transformation of glutamate-producing bacteria with plasmid DNA", J. BACTERIOL., vol. 159, 1984, pages 306 - 311
EIKMANNS, B. J. ET AL.: "A family of Corynebacterium glutamicum/Escherichia coli shuttle vectors for cloning, controlled gene expression, and promoter probing", GENE, vol. 102, 1991, pages 93 - 98, XP023541652, DOI: doi:10.1016/0378-1119(91)90545-M
KURUSU, Y. ET AL.: "Electroporation-transformation system for Coryneform bacteria by auxotrophic complementation.", AGRIC. BIOL. CHEM., vol. 54, 1990, pages 443 - 447
VERTES A.A. ET AL.: "Presence of mrr- and mcr-like restriction systems in Coryneform bacteria", RES. MICROBIOL., vol. 144, 1993, pages 181 - 185, XP023924914, DOI: doi:10.1016/0923-2508(93)90043-2
INUI, M. ET AL.: "Metabolic analysis of Corynebacterium glutamicum during lactate and succinate productions under oxygen deprivation conditions", J. MOL. MICROBIOL. BIOTECHNOL., vol. 7, 2004, pages 182 - 196, XP009070835, DOI: doi:10.1159/000079827
C. A ET AL.: "Corynebacterium glutamicumglyceraldehyde-3-phosphate dehydrogenase isoforms with opposite, ATP-dependent regulation.", J. MOL. MICROBIOL. BIOTECHNOL., vol. 8, 2004, pages 91 - 103
J. MOL. MICROBIOL. BIOTECHNOL., vol. 8, 2004, pages 243 - 254
OMUMASABA, C. A. ET AL.: "Corynebacterium glutamicum glyceraldehyde-3-phosphate dehydrogenase isoforms with opposite, ATP-dependent regulation", J. MOL. MICROBIOL. BIOTECHNOL., vol. 8, 2004, pages 91 - 103
PFENNIG, N. ET AL.: "The Prokaryotes, A Handbook on Habitats, Isolation and Identification of Bacteria", 1981, SPRINGER VERLAG, article "The dissimilatory sulfate-reducing bacteria", pages: 926 - 940
"Nogeikagaku Jikkensho", vol. 3, 1990, SANGYO TOSHO, pages: 26
OMUMASABA, C. A. ET AL.: "Corynebacterium glutamicum glyceraldehyde-3-phosphate dehydrogenase isoforms with opposite, ATP-dependent regulation.", J. MOL. MICROBIOL. BIOTECHNOL., vol. 8, 2004, pages 91 - 103
JOURNAL OF MOLECULAR BIOLOGY, vol. 53, 1970, pages 159
J. MOL. BIOTECHNOL., vol. 8, 2004, pages 243 - 254
AGRIC. BIOL. CHEM., vol. 54, 1990, pages 443 - 447
RES. MICROBIOL., vol. 144, 1993, pages 181 - 185
See also references of EP 2749638A4
Attorney, Agent or Firm:
IWATANI, Ryo (JP)
Tatsu Iwatani (JP)
Tatsu Iwatani (JP)
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