Title:
TRANSFORMANT AND METHOD FOR PRODUCING 3-HYDROXYPROPIONIC ACID
Document Type and Number:
WIPO Patent Application WO/2018/030526
Kind Code:
A1
Abstract:
Provide are a transformant of S.pombe enabling production of 3-HP with a high productivity without requiring neutralization with an alkali, and a method for producing 3-HP by culturing said transformant. The transformant uses Schizosaccharomyces pombe as the host, and is characterized in that a malonyl-CoA reductase coding gene, an acetyl-CoA carboxylase coding gene, a pyruvate oxidase coding gene, and an acetyl-CoA synthetase coding gene are introduced, or in that a malonyl-CoA reductase coding gene, an acetyl-CoA carboxylase coding gene and a gene cluster that may form a pyruvate dehydrogenase complex are introduced, said pyruvate oxydase being an enzyme that catalyzes acetic acid production reaction from pyruvic acid and water.
Inventors:
URUSHIHARA MASAHIRO (JP)
TAKEGAWA KAORU (JP)
SUYAMA AKIKO (JP)
TAKEGAWA KAORU (JP)
SUYAMA AKIKO (JP)
Application Number:
PCT/JP2017/029169
Publication Date:
February 15, 2018
Filing Date:
August 10, 2017
Export Citation:
Assignee:
ASAHI GLASS CO LTD (JP)
UNIV KYUSHU NAT UNIV CORP (JP)
UNIV KYUSHU NAT UNIV CORP (JP)
International Classes:
C12N15/09; C12N1/19; C12P7/42; C12P7/52
Domestic Patent References:
| WO2016084857A1 | 2016-06-02 | |||
| WO2015057154A2 | 2015-04-23 | |||
| WO2015087327A1 | 2015-06-18 |
Foreign References:
| JP2013535203A | 2013-09-12 | |||
| JP2015506681A | 2015-03-05 | |||
| JP2016511630A | 2016-04-21 |
Other References:
KAORU TAKEGAWA: "Kobo o Riyo shite Yuyo Yukisan o Tairyo Seizo suru Gijutsu", TECHNOLOGY SEEDS OF KYUSHU UNIVERSITY, 29 March 2016 (2016-03-29), Retrieved from the Internet [retrieved on 20171108]
KILDEGAARD, K. R. ET AL.: "Engineering and systems-level analysis of Saccharomyces cerevisiae for production of 3-hydroxypropionic acid via malonyl-CoA reductase-dependent pathway", MICROBIAL CELL FACTORIES, vol. 15, 15 March 2016 (2016-03-15), pages 1 - 13, XP055434334, ISSN: 1475-2859
SUYAMA, A. ET AL.: "Production of 3- hydroxypropionic acid via the malonyl-CoA pathway using recombinant fission yeast strains", JOURNAL OF BIOSCIENCE AND BIOENGINEERING, vol. 124, no. 4, 15 May 2017 (2017-05-15), pages 392 - 399, XP085169353, ISSN: 1389-1723
KILDEGAARD, K. R. ET AL.: "Engineering and systems-level analysis of Saccharomyces cerevisiae for production of 3-hydroxypropionic acid via malonyl-CoA reductase-dependent pathway", MICROBIAL CELL FACTORIES, vol. 15, 15 March 2016 (2016-03-15), pages 1 - 13, XP055434334, ISSN: 1475-2859
SUYAMA, A. ET AL.: "Production of 3- hydroxypropionic acid via the malonyl-CoA pathway using recombinant fission yeast strains", JOURNAL OF BIOSCIENCE AND BIOENGINEERING, vol. 124, no. 4, 15 May 2017 (2017-05-15), pages 392 - 399, XP085169353, ISSN: 1389-1723
Attorney, Agent or Firm:
SENMYO Kenji et al. (JP)
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