Title:
TRANSFORMANT, AND METHOD USING SAID TRANSFORMANT TO DETECT PRESENCE OR ABSENCE OF REDUCED PHOSPHOROUS COMPOUND
Document Type and Number:
WIPO Patent Application WO/2019/168163
Kind Code:
A1
Abstract:
An objective of the present invention is to provided transformants of various species that depend on phosphorous acid for growth, and a method using said transformants to detect the presence or absence of reduced phosphorous compounds. The present invention uses a transformant in which the function of genes that code phosphoric acid transporter proteins and phosphoric acid ester transporter proteins is missing, and into which a gene that codes hypophosphite transporter proteins has been introduced, the signal peptides of the hypophosphite binding proteins being replaced with signal peptides of the host or a species closely related to the host.
Inventors:
HIROTA RYUICHI (JP)
KURODA AKIO (JP)
MOTOMURA KEI (JP)
KURODA AKIO (JP)
MOTOMURA KEI (JP)
Application Number:
PCT/JP2019/008137
Publication Date:
September 06, 2019
Filing Date:
March 01, 2019
Export Citation:
Assignee:
UNIV HIROSHIMA (JP)
International Classes:
C12N1/13; C12Q1/04; C12N15/53
Domestic Patent References:
| WO2014024998A1 | 2014-02-13 | |||
| WO2018042987A1 | 2018-03-08 |
Other References:
KANABARA, AKIHIRO ET AL.: "Development of selective culture method for cyanobacteria using phosphorous acid", PROCEEDINGS OF THE 69TH ANNUAL MEETING OF THE JAPANESE SOCIETY FOR BIOTECHNOLOGY, vol. 69, 8 August 2017 (2017-08-08), Japan, pages 138, XP009523472
ANDREA WHITE , WILLIAM W METCALL : "The htx and ptx Operons of Pseudomonas stutzeri WM88 Are New Members of the Pho Regulon", JOURNAL OF BACTERIOLOGY, vol. 186, no. 17, 18 August 2004 (2004-08-18), pages 5876 - 5882, XP055470749, ISSN: 0021-9193, DOI: 10.1128/JB.186.17.5876-5882.2004
RYUICHI HIROTA , AKIO KURODA : "Discovery of a Thermostable Phosphite Dehydrogenase and Its Application", JOURNAL OF ENVIRONMENTAL BIOTECHNOLOGY, vol. 14, no. 1, 2014, JP, pages 15 - 20, XP009523310, ISSN: 1347-1856
MARY M MACKLE , BARBARA A ZILINSKAS : "Role of signal peptides in targeting of proteins in cyanobacteria", JOURNAL OF BACTERIOLOGY, vol. 176, no. 7, 1 April 1994 (1994-04-01), pages 1857 - 1864, XP055634403, ISSN: 1857-1864, DOI: 10.1128/jb.176.7.1857-1864.1994
RAMESH K. VUPPADA, COLBY R. HANSEN, KIRSTA A. P. STRICKLAND, KEILEN M. KELLY AND WILLIAM R. MCCLEARY: "Phosphate signaling through alternate conformations of the PstSCAB phosphate transporter", BMC MICROBIOLOGY, vol. 18, no. 8, 19 January 2018 (2018-01-19), pages 1 - 9, XP055634405, DOI: 10.1186/s12866-017-1126-z
RYUICHI HIROTA , AKIO KURODA , KEI MOTOMURA : "Development of biosafety technology for using the first type of recombinant microalgae - Biological containment using phosphorous acid dependence", CHEMICAL ENGINEERING, vol. 63, no. 11, 1 November 2018 (2018-11-01), pages 799 - 805, XP009523461, ISSN: 0387-1037
KOSUKE SANO , KEI MOTOMURA , AKIHIRO KANBARA , SATORU WANTANABE , TAKESHI IKEDA , TAKENORI ISHIDA , HISAKAGE FUNABASHI , AKIO KURO: "An engineered phosphite metabolic pathway for a biosafety system of cyanobacteria", LECTURE ABSTRACTS OF THE 70TH ANNUAL MEETING OF THE SOCIETY FOR BIOTECHNOLOGY , vol. 70, 5 September 2019 (2019-09-05), Japan, pages 1 - 2, XP009523507
MOTOMURA, KEI; HIROTA, RYUICHI; SANO, KOSUKE; KANBARA, AKIHIRO; KATSUURA, ZEN-ICHIRO; HORIKAWA, RYOHEI; WATANABE, SATORU; IKEDA, T: "2A02a06 A novel biological containment system for genetically engineered cyanobacteria based on phosphite auxotrophy", ANNUAL MEETING OF THE JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY AND AGROCHEMISTRY, 2018 (JSBBA 2018), vol. 2018, 5 March 2018 (2018-03-05), pages 122, XP009523457
KEI MOTOMURA , AKIHIRO KANBARA , RYOHEI HORIKAWA , TAKESHI IKEDA , TAKENORI ISHIDA , HISAKAGE FUNABASHI , AKIO KURODA , RYUICHI HI: "Enzymatic properties of phosphite dehydrogenase from cyanobacteria and its application as a dominant selection marker", LECTURE ABSTRACTS OF THE 70TH ANNUAL MEETING OF THE SOCIETY FOR BIOTECHNOLOGY, vol. 70, 5 September 2018 (2018-09-05), Japan, pages 1 - 2, XP009523508
RYUICHI HIROTA: "A Novel Biocontainment Strategy Makes Bacterial Growth and Survival Dependent on Phosphite", SCIENTIFIC REPORTS, 20 March 2017 (2017-03-20)
"Kazusa Genome Resource", article "PCC 7942"
MOTOMURA, K. ET AL.: "Overproduction of YjbB reduces the level of polyphosphate in Escherichia coli: a hypothetical role of YjbB in phosphate export and polyphosphate accumulation", FEMS MICROBIOLOGY LETTERS, vol. 320, 2011, pages 25 - 32, XP055604202, DOI: 10.1111/j.1574-6968.2011.02285.x
SAMBROOK ET AL.: "Molecular Cloning, A Laboratory Manual", 2001, COLD SPRING HARBOR LABORATORY
WATANABE ET AL., MOL MICROBIOL., vol. 83, no. 4, February 2012 (2012-02-01), pages 856 - 65
HIROTA ET AL., SCI. REP., 2017, pages 44748
See also references of EP 3760706A4
ANDREA WHITE , WILLIAM W METCALL : "The htx and ptx Operons of Pseudomonas stutzeri WM88 Are New Members of the Pho Regulon", JOURNAL OF BACTERIOLOGY, vol. 186, no. 17, 18 August 2004 (2004-08-18), pages 5876 - 5882, XP055470749, ISSN: 0021-9193, DOI: 10.1128/JB.186.17.5876-5882.2004
RYUICHI HIROTA , AKIO KURODA : "Discovery of a Thermostable Phosphite Dehydrogenase and Its Application", JOURNAL OF ENVIRONMENTAL BIOTECHNOLOGY, vol. 14, no. 1, 2014, JP, pages 15 - 20, XP009523310, ISSN: 1347-1856
MARY M MACKLE , BARBARA A ZILINSKAS : "Role of signal peptides in targeting of proteins in cyanobacteria", JOURNAL OF BACTERIOLOGY, vol. 176, no. 7, 1 April 1994 (1994-04-01), pages 1857 - 1864, XP055634403, ISSN: 1857-1864, DOI: 10.1128/jb.176.7.1857-1864.1994
RAMESH K. VUPPADA, COLBY R. HANSEN, KIRSTA A. P. STRICKLAND, KEILEN M. KELLY AND WILLIAM R. MCCLEARY: "Phosphate signaling through alternate conformations of the PstSCAB phosphate transporter", BMC MICROBIOLOGY, vol. 18, no. 8, 19 January 2018 (2018-01-19), pages 1 - 9, XP055634405, DOI: 10.1186/s12866-017-1126-z
RYUICHI HIROTA , AKIO KURODA , KEI MOTOMURA : "Development of biosafety technology for using the first type of recombinant microalgae - Biological containment using phosphorous acid dependence", CHEMICAL ENGINEERING, vol. 63, no. 11, 1 November 2018 (2018-11-01), pages 799 - 805, XP009523461, ISSN: 0387-1037
KOSUKE SANO , KEI MOTOMURA , AKIHIRO KANBARA , SATORU WANTANABE , TAKESHI IKEDA , TAKENORI ISHIDA , HISAKAGE FUNABASHI , AKIO KURO: "An engineered phosphite metabolic pathway for a biosafety system of cyanobacteria", LECTURE ABSTRACTS OF THE 70TH ANNUAL MEETING OF THE SOCIETY FOR BIOTECHNOLOGY , vol. 70, 5 September 2019 (2019-09-05), Japan, pages 1 - 2, XP009523507
MOTOMURA, KEI; HIROTA, RYUICHI; SANO, KOSUKE; KANBARA, AKIHIRO; KATSUURA, ZEN-ICHIRO; HORIKAWA, RYOHEI; WATANABE, SATORU; IKEDA, T: "2A02a06 A novel biological containment system for genetically engineered cyanobacteria based on phosphite auxotrophy", ANNUAL MEETING OF THE JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY AND AGROCHEMISTRY, 2018 (JSBBA 2018), vol. 2018, 5 March 2018 (2018-03-05), pages 122, XP009523457
KEI MOTOMURA , AKIHIRO KANBARA , RYOHEI HORIKAWA , TAKESHI IKEDA , TAKENORI ISHIDA , HISAKAGE FUNABASHI , AKIO KURODA , RYUICHI HI: "Enzymatic properties of phosphite dehydrogenase from cyanobacteria and its application as a dominant selection marker", LECTURE ABSTRACTS OF THE 70TH ANNUAL MEETING OF THE SOCIETY FOR BIOTECHNOLOGY, vol. 70, 5 September 2018 (2018-09-05), Japan, pages 1 - 2, XP009523508
RYUICHI HIROTA: "A Novel Biocontainment Strategy Makes Bacterial Growth and Survival Dependent on Phosphite", SCIENTIFIC REPORTS, 20 March 2017 (2017-03-20)
"Kazusa Genome Resource", article "PCC 7942"
MOTOMURA, K. ET AL.: "Overproduction of YjbB reduces the level of polyphosphate in Escherichia coli: a hypothetical role of YjbB in phosphate export and polyphosphate accumulation", FEMS MICROBIOLOGY LETTERS, vol. 320, 2011, pages 25 - 32, XP055604202, DOI: 10.1111/j.1574-6968.2011.02285.x
SAMBROOK ET AL.: "Molecular Cloning, A Laboratory Manual", 2001, COLD SPRING HARBOR LABORATORY
WATANABE ET AL., MOL MICROBIOL., vol. 83, no. 4, February 2012 (2012-02-01), pages 856 - 65
HIROTA ET AL., SCI. REP., 2017, pages 44748
See also references of EP 3760706A4
Attorney, Agent or Firm:
HARAKENZO WORLD PATENT & TRADEMARK (JP)
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