Title:
TRANSFORMANT WHICH PRODUCES COLLAGEN WHEREIN BOTH LYSINE RESIDUE AND PROLINE RESIDUE ARE HYDROXYLATED
Document Type and Number:
WIPO Patent Application WO/2011/059092
Kind Code:
A1
Abstract:
Disclosed is a transformant which is obtained by introducing all of the foreign genes (1)-(3) described below into a microbial cell in order to obtain a collagen that can be a high-performance versatile material which is commercially more valuable as a pharmaceutical product, industrial product, cosmetic product, food and the like.
(1) A foreign gene comprising a base sequence encoding the amino acid sequence of lysine hydroxylase
(2) A foreign gene comprising a base sequence encoding the amino acid sequence of proline hydroxylase
(3) A foreign gene comprising a base sequence encoding the amino acid sequence of a collagen
Inventors:
NISHIO SHOICHI (JP)
Application Number:
PCT/JP2010/070356
Publication Date:
May 19, 2011
Filing Date:
November 16, 2010
Export Citation:
Assignee:
SUMITOMO CHEMICAL CO (JP)
KOKEN KK (JP)
DAINIPPON SUMITOMO PHARMA CO (JP)
NISHIO SHOICHI (JP)
KOKEN KK (JP)
DAINIPPON SUMITOMO PHARMA CO (JP)
NISHIO SHOICHI (JP)
International Classes:
C12N15/09; C07K14/78; C12N1/19; C12P21/02
Domestic Patent References:
| WO1997038710A1 | 1997-10-23 |
Foreign References:
| JP2001502548A | 2001-02-27 |
Other References:
MILLER EJ. ET AL.: "Collagen: An overview", METH. ENZYMOL., vol. 82, 1982, pages 3 - 32, XP008159804
NOKELAINEN M. ET AL.: "High-level production of human type I collagen in the yeast Pichia pastoris", YEAST, vol. 18, no. 9, 2001, pages 797 - 806, XP008159810
STEIN H. ET AL.: "Production of bioactive, post-translationally modified, heterotrimeric, human recombinant type-I collagen in transgenic tobacco", BIOMACROMOLECULES, vol. 10, no. 9, September 2009 (2009-09-01), pages 2640 - 2645, XP008159867
See also references of EP 2502991A4
MASAO TANIHARA: "Production and Development of Applications of Collagen", CMC PUBLISHING CO., LTD.
"Topics in Current Chemistry", vol. 247, 2005, article "Collagen primer in Structure, Processing and Assembly"
GELSE, K.; POSCHL, E.; AIGNER, T.: "Collagens-structure, function, and biosynthesis", ADVANCED DRUG DELIVERY REVIEWS, vol. 55, 2003, pages 1531 - 1546
MILLER ET AL.: "Methods In Enzymology", vol. 82, 1982, ACADEMIC PRESS, pages: 33 - 64
BYERS ET AL., BIOCHEMISTRY, vol. 13, 1974, pages 5243 - 5248
P. DAVID TOMAN ET AL., J. BIOL. CHEM., vol. 275, no. 30, 28 July 2000 (2000-07-28), pages 23303 - 23309
TOWBIN ET AL., PROC. NATL. ACAD. SCI. USA, vol. 76, 1979, pages 4350 - 4354
"Molecular Cloning: A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
"Current Protocols in Molecular Biology", 1987, JOHN WILEY & SONS
LAEMMLI, U. K., NATURE, vol. 227, 1970, pages 680
LAEMMLI, U. K, NATURE, vol. 227, 1970, pages 680
LAEMMII, U. K., NATURE, vol. 227, 1970, pages 680
NOKELAINEN M. ET AL.: "High-level production of human type I collagen in the yeast Pichia pastoris", YEAST, vol. 18, no. 9, 2001, pages 797 - 806, XP008159810
STEIN H. ET AL.: "Production of bioactive, post-translationally modified, heterotrimeric, human recombinant type-I collagen in transgenic tobacco", BIOMACROMOLECULES, vol. 10, no. 9, September 2009 (2009-09-01), pages 2640 - 2645, XP008159867
See also references of EP 2502991A4
MASAO TANIHARA: "Production and Development of Applications of Collagen", CMC PUBLISHING CO., LTD.
"Topics in Current Chemistry", vol. 247, 2005, article "Collagen primer in Structure, Processing and Assembly"
GELSE, K.; POSCHL, E.; AIGNER, T.: "Collagens-structure, function, and biosynthesis", ADVANCED DRUG DELIVERY REVIEWS, vol. 55, 2003, pages 1531 - 1546
MILLER ET AL.: "Methods In Enzymology", vol. 82, 1982, ACADEMIC PRESS, pages: 33 - 64
BYERS ET AL., BIOCHEMISTRY, vol. 13, 1974, pages 5243 - 5248
P. DAVID TOMAN ET AL., J. BIOL. CHEM., vol. 275, no. 30, 28 July 2000 (2000-07-28), pages 23303 - 23309
TOWBIN ET AL., PROC. NATL. ACAD. SCI. USA, vol. 76, 1979, pages 4350 - 4354
"Molecular Cloning: A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
"Current Protocols in Molecular Biology", 1987, JOHN WILEY & SONS
LAEMMLI, U. K., NATURE, vol. 227, 1970, pages 680
LAEMMLI, U. K, NATURE, vol. 227, 1970, pages 680
LAEMMII, U. K., NATURE, vol. 227, 1970, pages 680
Attorney, Agent or Firm:
TANAKA, Mitsuo et al. (JP)
Mitsuo Tanaka (JP)
Mitsuo Tanaka (JP)
Download PDF: