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Title:
TRANSFORMED FUNGUS HAVING ENHANCED ERGOTHIONEINE PRODUCTIVITY AND METHOD FOR PRODUCING ERGOTHIONEINE
Document Type and Number:
WIPO Patent Application WO/2016/121285
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide an organism having an ergothioneine productivity that is capable of easily producing ergothioneine within a short period of time at a high yield, as compared with a conventional technology, and, therefore, enables ergothioneine production on an industrial scale. This purpose can be achieved by a transformed fungus into which a gene encoding enzyme (1) or genes encoding enzymes (1) and (2) have been inserted and in which the inserted gene(s) are overexpressed. (1) An enzyme catalyzing a reaction of synthesizing hercynyl cysteine sulfoxide from histidine and cysteine in the presence of S-adenosyl methionine, iron (II) and oxygen. (2) An enzyme catalyzing a reaction of synthesizing ergothioneine from hercynyl cysteine sulfoxide using pyridoxal 5'-phosphate as a coenzyme.

Inventors:
HARA SEIICHI (JP)
KUROSAWA KEIKO (JP)
ICHIKAWA KEIICHI (JP)
Application Number:
PCT/JP2015/086301
Publication Date:
August 04, 2016
Filing Date:
December 25, 2015
Export Citation:
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Assignee:
KIKKOMAN CORP (JP)
International Classes:
C12N1/15; C12N9/10; C12N15/09; C12P13/04; C12R1/66; C12R1/665; C12R1/685; C12R1/69
Domestic Patent References:
WO2014100752A12014-06-26
Other References:
GENGHOF, D. S.: "Biosynthesis of ergothioneine and hercynine by fungi and Actinomycetales", J. BACTERIOL., vol. 103, no. 2, 1970, pages 475 - 478, XP055364661, ISSN: 0021-9193
MELVILLE , D. B. ET AL.: "Ergothioneine in microorganisms.", J. BIOL. CHEM., vol. 223, 1956, pages 9 - 17, XP055364665, ISSN: 0021-9258
VIT, A. ET AL.: "Ergothioneine biosynthetic methyltransferase EgtD reveals the structural basis of aromatic amino acid betaine biosynthesis", CHEMBIOCHEM, vol. 16, 2014, pages 119 - 125, XP055364667, ISSN: 1439-7633
PLUSKAL, T. ET AL.: "Genetic and metabolomic dissection of the ergothioneine and selenoneine biosynthetic pathway in the fission yeast, S. pombe, and construction of an overproduction system", PLOS ONE, vol. 9, no. 5, e97774, 2014, pages 1 - 12, XP055364654, ISSN: 1932-6203
DATABASE GenBank [o] 4 June 2011 (2011-06-04), XP055455356, Database accession no. BAE60470
DATABASE GenBank [o] 12 October 2011 (2011-10-12), XP055455400, Database accession no. EHA24811
DATABASE GenBank [o] 16 May 2014 (2014-05-16), XP055455403, Database accession no. KDE85407
DATABASE GenBank [o] 18 June 2012 (2012-06-18), XP055455406, Database accession no. EIT79021
DATABASE GenBank [o] 18 June 2012 (2012-06-18), XP055461317, Database accession no. EIT76462
BELLO, M. H. ET AL.: "The Neurospora crassa mutant NcDELTAEgt-1 identifies an ergothioneine biosynthetic gene and demonstrates that ergothioneine enhances conidial survival and protects against peroxide toxicity during conidial germination", FUNGAL GENET. BIOL., vol. 49, 2012, pages 160 - 172, XP055266311, ISSN: 1087-1845
See also references of EP 3252142A4
Attorney, Agent or Firm:
MORIMOTO, TOSHIAKI (JP)
Toshiaki Morimoto (JP)
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