Title:
TRANSGENIC BIRD CAPABLE OF EXPRESSING α-GALACTOSE EPITOPE, VIRUS, AND VACCINE
Document Type and Number:
WIPO Patent Application WO/2011/040527
Kind Code:
A1
Abstract:
Disclosed are: a means for expressing α-Gal in a virus without using any enzyme; the production of a virus having α-Gal expressed therein by utilizing the means; the production of a vaccine from the virus; an influenza virus vaccine having a high effect (antigenicity), which is produced using a virus having enhanced immune response to a virus; and others. Specifically disclosed are: a transgenic bird which can express an α-galactose epitope (Galα1-3Galβ1-4GlcNAc-R: referred to as "α-Gal", hereinafter), wherein the bird is a chicken and may be G0 or a progeny thereof; a method for producing a transgenic bird, which comprises introducing a vector that is used for the introduction of an α1,3-galactose transferase (α1,3-GT) gene and contains an α1,3-GT gene in such a manner that the gene can be expressed, into a bird, thereby producing the transgenic bird; a biological sample, such as an egg, obtained from the transgenic bird; and a method for producing an α-Gal-expressing virus, which comprises inoculating a biological sample with a virus, cultivating the biological sample that has been inoculated with the virus, and obtaining a virus capable of expressing α-Gal from the cultivated biological sample.
Inventors:
OGAWA HARUKO (JP)
TAGAMI TAKAHIRO (JP)
TAGAMI TAKAHIRO (JP)
Application Number:
PCT/JP2010/067083
Publication Date:
April 07, 2011
Filing Date:
September 30, 2010
Export Citation:
Assignee:
NAT UNIVERSITY CORP OBIHIRO UNIVERSITY OF AGRICULTURE AND VETERINARY MEDICINE (JP)
INC ADMIN AGENCY NARO (JP)
OGAWA HARUKO (JP)
TAGAMI TAKAHIRO (JP)
INC ADMIN AGENCY NARO (JP)
OGAWA HARUKO (JP)
TAGAMI TAKAHIRO (JP)
International Classes:
A01K67/027; A61K39/12; A61K39/145; A61K39/165; A61K39/17; A61K39/20; A61K39/21; A61K39/255; A61K39/285; A61P31/12; A61P31/16; A61P31/18; C12N7/00; C12N15/09
Domestic Patent References:
| WO2005007827A2 | 2005-01-27 | |||
| WO1995003399A2 | 1995-02-02 |
Foreign References:
| US20090123494A1 | 2009-05-14 | |||
| EP1939281A1 | 2008-07-02 |
Other References:
ABDEL-MOTAL, UM. ET AL.: "Immunogenicity ot influenza virus vaccine is increased by anti-gal-mediated targeting to antigen- presenting cells.", J VIROL., vol. 81, 2007, pages 9131 - 9141, XP008155433, DOI: doi:10.1128/JVI.00647-07
PREECE, AF. ET AL.: "Expression of ABO or related antigenic carbohydrates on viral envelopes leads to neutralization in the presence of serum containing specific natural antibodies and complement.", BLOOD, vol. 99, 2002, pages 2477 - 2482, XP008155431, DOI: doi:10.1182/blood.V99.7.2477
MCGREW, MJ. ET AL.: "Efficient production of germline transgenic chickens using lentiviral vectors.", EMBO REP., vol. 5, 2004, pages 728 - 733, XP002341505, DOI: doi:10.1038/sj.embor.7400171
PREECE, AF. ET AL.: "Expression of ABO or related antigenic carbohydrates on viral envelopes leads to neutralization in the presence of serum containing specific natural antibodies and complement.", BLOOD, vol. 99, 2002, pages 2477 - 2482, XP008155431, DOI: doi:10.1182/blood.V99.7.2477
MCGREW, MJ. ET AL.: "Efficient production of germline transgenic chickens using lentiviral vectors.", EMBO REP., vol. 5, 2004, pages 728 - 733, XP002341505, DOI: doi:10.1038/sj.embor.7400171
Attorney, Agent or Firm:
SIKs & Co. (JP)
Patent business corporation patent firm Sykes (JP)
Patent business corporation patent firm Sykes (JP)
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