Title:
TRANSGENIC METHOD OF OBTAINING BLUE FLOWERS BY CATALYZING GLUTAMINE TO SYNTHESIZE INDIGO
Document Type and Number:
WIPO Patent Application WO/2019/148944
Kind Code:
A1
Abstract:
A transgenic method of obtaining blue flowers by catalyzing glutamine to synthesize indigo is provided. The steps thereof comprise: 1) respectively cloning a Sfp gene encoding phosphopantetheinyl transferase and a bpsA gene encoding indigo synthase downstream of a plant promoter in a plant-promoter-containing plasmid; 2) amplifying the obtained plasmid in E. coli and then transferring the same to Agrobacterium tumefaciens; and 3) transferring DNA containing Sfp and bpsA into a plant. The blue flowers produced by the present invention have various characteristics of natural flowers, being fresh, flower-scented, non- color-fading, and non-toxic. The transgene-encoded enzyme and the produced indigo are not in the vacuole and are not affected by the low pH of the plant vacuole, thereby resulting in a pure blue color. The precursor of the blue matter, i.e., the substrate of the enzyme, is glutamine, which is abundant in plants. The enzyme catalysis reaction comprises a single step, and the transgenic transformation can be carried out on natural white flowers.
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Inventors:
ZHANG YAN (CN)
CHEN YIHUA (CN)
ANKANAHALLI NANJARAJ URS (CN)
HU YILING (CN)
LI PENGWEI (CN)
CHEN YIHUA (CN)
ANKANAHALLI NANJARAJ URS (CN)
HU YILING (CN)
LI PENGWEI (CN)
Application Number:
PCT/CN2018/117160
Publication Date:
August 08, 2019
Filing Date:
November 23, 2018
Export Citation:
Assignee:
UNIV TIANJIN (CN)
INST MICROBIOLOGY CAS (CN)
INST MICROBIOLOGY CAS (CN)
International Classes:
C12N15/82; A01H5/00; A01H6/30; A01H6/56; A01H6/62; A01H6/74; C12N15/54; C12N15/60
Foreign References:
| CN108330146A | 2018-07-27 | |||
| CN1189856A | 1998-08-05 | |||
| CN1970642A | 2007-05-30 |
Other References:
NANJARAJ URS, A.N. ET AL.: "Cloning and Expression of a Nonribosomal Peptide Synthetase to Generate Blue Rose", ACS SYNTHETIC BIOLOGY, vol. 8, 14 September 2018 (2018-09-14), XP055630157, ISSN: 2161-5063
T AKAHASHI, H. ET AL.: "Cloning and Characterization of a Streptomyces Single Module Type Nonribosomal Peptide Synthetase Catalyzing a Blue Pigment Synthesis", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 282, no. 12, 19 January 2017 (2017-01-19), XP055068798, ISSN: 0021-9258, doi:10.1074/jbc.M611319200
GROTEWOLD, E.: "The genetics and biochemistry of floral Pigments", ANNUAL REVIEW OF PLANT BIOLOGY, vol. 57, no. 1, 2006, pages 761
HOLTON T.A: "Tanaka Y Blue roses: a pigment of our imagination?", TRENDS IN BIOTECHNOLOGY: UNITED KINGDOM, vol. 12, no. 2, 1994, pages 40 - 42, XP023595397, DOI: 10.1016/0167-7799(94)90097-3
TANAKA Y: "Brugliera F. Flower colour and cytochromes P450", PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON, vol. 368, no. 1612, 2013, pages 283 - 291
MIKANAGI YSAITO NYOKOI M ET AL.: "Anthocyanins in flowers of genus Rosa, sections Cinnamomeae (=Rosa), Chinenses, Gallicanae and some modern garden roses", BIOCHEMICAL SYSTEMATICS & ECOLOGY, vol. 28, no. 9, 2000, pages 887
KATSUMOTO YFUKUCHIMIZUTANI MFUKUI Y ET AL.: "Engineering of the rose flavonoid biosynthetic pathway successfully generated blue-hued flowers accumulating delphinidin", PLANT & CELL PHYSIOLOGY, vol. 48, no. 11, 2007, pages 1589, XP009133724, DOI: 10.1093/pcp/pcm131
"NCBI", Database accession no. WP _030237949
T AKAHASHI, H. ET AL.: "Cloning and Characterization of a Streptomyces Single Module Type Nonribosomal Peptide Synthetase Catalyzing a Blue Pigment Synthesis", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 282, no. 12, 19 January 2017 (2017-01-19), XP055068798, ISSN: 0021-9258, doi:10.1074/jbc.M611319200
GROTEWOLD, E.: "The genetics and biochemistry of floral Pigments", ANNUAL REVIEW OF PLANT BIOLOGY, vol. 57, no. 1, 2006, pages 761
HOLTON T.A: "Tanaka Y Blue roses: a pigment of our imagination?", TRENDS IN BIOTECHNOLOGY: UNITED KINGDOM, vol. 12, no. 2, 1994, pages 40 - 42, XP023595397, DOI: 10.1016/0167-7799(94)90097-3
TANAKA Y: "Brugliera F. Flower colour and cytochromes P450", PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON, vol. 368, no. 1612, 2013, pages 283 - 291
MIKANAGI YSAITO NYOKOI M ET AL.: "Anthocyanins in flowers of genus Rosa, sections Cinnamomeae (=Rosa), Chinenses, Gallicanae and some modern garden roses", BIOCHEMICAL SYSTEMATICS & ECOLOGY, vol. 28, no. 9, 2000, pages 887
KATSUMOTO YFUKUCHIMIZUTANI MFUKUI Y ET AL.: "Engineering of the rose flavonoid biosynthetic pathway successfully generated blue-hued flowers accumulating delphinidin", PLANT & CELL PHYSIOLOGY, vol. 48, no. 11, 2007, pages 1589, XP009133724, DOI: 10.1093/pcp/pcm131
"NCBI", Database accession no. WP _030237949
Attorney, Agent or Firm:
BEI & OCEAN (CN)
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