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Title:
TRANSGENIC PLANT EXHIBITING ENHANCED GROWTH AND METHOD FOR PRODUCING SAME
Document Type and Number:
WIPO Patent Application WO/2019/142916
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing a plant which exhibits an enhanced growing ability compared to plants produced by conventional methods, by establishing a method for producing a transgenic plant which exhibits an enhanced growing ability. A transgenic plant which exhibits a growing ability which is enhanced compared to that of the host plant, and has a chimeric protein of a peptide containing an amino acid sequence derived from a motor domain of myosin XI of a donor plant 1, which is a plant species other than the host plant, and a peptide containing an amino acid sequence derived from a domain other than the motor domain of myosin XI of a donor plant 2, which is the host plant or a plant species other than the host plant, the transgenic plant being characterized in that the motor domain loop 2 region has EEPKQGGKGGGKSSFSSIG or EEPKQGGGKGGSKSSFSSIG, and in addition to these sequences, has an amino acid sequence in which multiple amino acids have been deleted, replaced or added.

Inventors:
TOMINAGA MOTOKI (JP)
ITO KOHJI (JP)
Application Number:
PCT/JP2019/001491
Publication Date:
July 25, 2019
Filing Date:
January 18, 2019
Export Citation:
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Assignee:
UNIV WASEDA (JP)
UNIV CHIBA NAT UNIV CORP (JP)
International Classes:
C12N15/11; A01H5/00; A01H6/46
Foreign References:
US20130007915A12013-01-03
Other References:
TOMINAGA, MOTOKI ET AL.: "Myosin Speed Determines Plant Size", SEIBUTSU BUTSURI, vol. 54, no. 5, 2014, pages 259 - 261
DUAN, ZHONGRUI ET AL.: "Technology for increasing production of biomass to manipulate protoplasmic streaming: Control plant size by rate modification of myosin XI", CHEMICAL INDUSTRY, vol. 68, no. 6, 2017, pages 444 - 448
TOMINAGA, MOTOKI: "Plant Light Strategy; A new point of view considering form growth control by cytoplasmic streaming", THE JAPANESE SOCIETY OF PHOTOSYNTHESIS RESEARCH, vol. 25, no. 1, 2015, pages 42 - 47
ITO, KOHJI: "Development of plant growth promotion system by protoplasmic streaming rate enhancement", ANNUAL REPORT OF VENTURE BUSINESS LABORATORY, CHIBA UNIVERSITY, vol. 15, 2015, pages 92 - 94
KASHIYAMA, T. ET AL.: "Functional expression of a chimeric myosin-containing motor domain of Chara myosin and neck and tail domains of Dictyostelium myosin II", J. MOL. BIOL., vol. 311, no. 3, 2001, pages 461 - 466, XP004472402, doi:10.1006/jmbi.2001.4883
ITO, K. ET AL.: "Unique charge distribution in surface loops confers high velocity on the fast motor protein Chara myosin", PROC. NATL. ACAD. SCI. USA, vol. 106, no. 51, 2009, pages 21585 - 21590, XP055627595
HIGASHI-FUJIME, S. ET AL.: "The fastest actin-based motor protein from the green algae, Chara, and its distinct mode of interaction with actin", FEBS LETT., vol. 375, no. 1-2, 1995, pages 151 - 154, XP055627599
Attorney, Agent or Firm:
USHIKI, Mamoru (JP)
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