Title:
TRANSGENIC SILKWORM AND METHOD FOR PRODUCING NON-NATURAL AMINO ACID-CONTAINING PROTEIN USING TRANSGENIC SILKWORM
Document Type and Number:
WIPO Patent Application WO/2018/190333
Kind Code:
A1
Abstract:
DNA which comprises an amino acid sequence containing any one of the following amino acid sequences (a) to (c) and encodes a protein, said protein having an activity of allowing a non-natural amino acid to bind to phenylalanine-specific tRNA, and a transgenic silkworm characterized by having a promoter which enables silk gland-specific expression of the aforesaid DNA: (a) an amino acid sequence having a mutation in the position corresponding to amino acid No. 432 of the amino acid sequence represented by SEQ ID NO: 1; (b) an amino acid sequence derived from the aforesaid amino acid sequence (a) by deletion, insertion, substitution or addition of one to several amino acids in position(s) other than the position corresponding to amino acid No. 432; and (c) an amino acid sequence having at least 80% identity with the aforesaid amino acid sequence (a) in positions other than the position corresponding to amino acid No. 432.
Inventors:
TERAMOTO HIDETOSHI (JP)
KOJIMA KATSURA (JP)
SAKAMOTO KENSAKU (JP)
KIMURA FUMIE (JP)
YAMAGUCHI YOSHIMI (JP)
KOJIMA KATSURA (JP)
SAKAMOTO KENSAKU (JP)
KIMURA FUMIE (JP)
YAMAGUCHI YOSHIMI (JP)
Application Number:
PCT/JP2018/015039
Publication Date:
October 18, 2018
Filing Date:
April 10, 2018
Export Citation:
Assignee:
NAT AGRICULTURE & FOOD RES ORG (JP)
RIKEN (JP)
RIKEN (JP)
International Classes:
C12N15/09; A01K67/033; C07K14/435
Foreign References:
| JP2015015927A | 2015-01-29 |
Other References:
FINAROV I. ET AL.: "Structure of Human Cytosolic Phenylalanyl-tRNA Synthetase: Evidence for Kingdom-Specific Design of the Active Sites and tRNA Binding Patterns", STRUCTURE, vol. 18, no. 3, 10 March 2010 (2010-03-10), pages 343 - 353, XP026941379, Retrieved from the Internet
TERAMOTO H. ET AL.: "Cloning of Bombyx mori phenylalanyl-tRNA synthetase and the generation of its mutant with relaxed amino acid specificity", J. INSECT BIOTECHNOL. SERICOL., vol. 79, no. 2, 2010, pages 2-53 - 2-65, XP055559426, Retrieved from the Internet
TERAMOTO H. ET AL.: "Production of Bombyx mori Silk Fibroin Incorporated with Unnatural Amino Acids", BIOMACROMOLECULES, vol. 15, no. 7, 2 June 2014 (2014-06-02), pages 2682 - 2690, XP055226031, Retrieved from the Internet
TERAMOTO H. ET AL.: "Residue-specific incorporation of phenylalanine analogues into protein biosynthesis in silkworm cultured cells", J INSECT BIOTECHNOL SERICOL, vol. 82, no. 3, 2013, pages 3-61 - 3-69, XP055559429, Retrieved from the Internet
TERAMOTO H. ET AL.: "Azide-Incorporated Clickable Silk Fibroin Materials with the Ability to Photopattern", ACS BIOMATER SCI ENG, vol. 2, no. 2, 11 January 2016 (2016-01-11), pages 251 - 258, XP055559433, Retrieved from the Internet
KWON L ET AL.: "Design of a Bacterial Host for Site-Specific Incorporation of p- Bromophenylalanine into Recombinant Proteins", J AM CHEM SOC, vol. 128, no. 36, 13 September 2006 (2006-09-13), pages 11778 - 11783, XP055559436, Retrieved from the Internet
KIRSHENBAUM K. ET AL.: "Biosynthesis of Proteins Incorporating a Versatile Set of Phenylalanine Analogues", CHEMBIOCHEM, vol. 3, no. 2-3, 7 March 2002 (2002-03-07), pages 235 - 237, XP055559440, Retrieved from the Internet3.0.CO;2-7>
TERAMOTO H. ET AL.: "Cloning of Bombyx mori phenylalanyl-tRNA synthetase and the generation of its mutant with relaxed amino acid specificity", J. INSECT BIOTECHNOL. SERICOL., vol. 79, no. 2, 2010, pages 2-53 - 2-65, XP055559426, Retrieved from the Internet
TERAMOTO H. ET AL.: "Production of Bombyx mori Silk Fibroin Incorporated with Unnatural Amino Acids", BIOMACROMOLECULES, vol. 15, no. 7, 2 June 2014 (2014-06-02), pages 2682 - 2690, XP055226031, Retrieved from the Internet
TERAMOTO H. ET AL.: "Residue-specific incorporation of phenylalanine analogues into protein biosynthesis in silkworm cultured cells", J INSECT BIOTECHNOL SERICOL, vol. 82, no. 3, 2013, pages 3-61 - 3-69, XP055559429, Retrieved from the Internet
TERAMOTO H. ET AL.: "Azide-Incorporated Clickable Silk Fibroin Materials with the Ability to Photopattern", ACS BIOMATER SCI ENG, vol. 2, no. 2, 11 January 2016 (2016-01-11), pages 251 - 258, XP055559433, Retrieved from the Internet
KWON L ET AL.: "Design of a Bacterial Host for Site-Specific Incorporation of p- Bromophenylalanine into Recombinant Proteins", J AM CHEM SOC, vol. 128, no. 36, 13 September 2006 (2006-09-13), pages 11778 - 11783, XP055559436, Retrieved from the Internet
KIRSHENBAUM K. ET AL.: "Biosynthesis of Proteins Incorporating a Versatile Set of Phenylalanine Analogues", CHEMBIOCHEM, vol. 3, no. 2-3, 7 March 2002 (2002-03-07), pages 235 - 237, XP055559440, Retrieved from the Internet
Attorney, Agent or Firm:
NISHIZAWA Kazuyoshi et al. (JP)
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