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Title:
TRIAZOLOPYRIMIDINE DERIVATIVES
Document Type and Number:
WIPO Patent Application WO/2001/019826
Kind Code:
A2
Abstract:
Compounds of the formula (I) and their use as anti-platelet aggregation compounds.

Inventors:
TEOBALD BARRY JOHN (GB)
Application Number:
PCT/GB2000/003474
Publication Date:
March 22, 2001
Filing Date:
September 11, 2000
Export Citation:
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Assignee:
ASTRAZENECA UK LTD (GB)
TEOBALD BARRY JOHN (GB)
International Classes:
A61K31/519; A61P1/04; A61P7/00; A61P7/02; A61P7/04; A61P9/00; A61P9/04; C07D239/47; A61P9/10; A61P11/00; A61P11/06; A61P13/12; A61P25/00; A61P25/06; A61P29/00; A61P35/00; A61P35/04; A61P37/06; A61P43/00; C07D207/14; C07D403/12; C07D487/04; (IPC1-7): C07D487/04; A61K31/519; A61P9/00; C07D403/12
Domestic Patent References:
WO1999005114A21999-02-04
WO1999005143A11999-02-04
WO2000034283A12000-06-15
Foreign References:
EP0508687A11992-10-14
Other References:
YEN-SHI LAI ET AL.: "Synthesis and protei kinase C inhibitory activities of lanol anaogs with replacement of the perhydroazepine moiety" JOURNAL OF MEDICINAL CHEMISTRY., vol. 40, no. 2, 1997, pages 226-35, XP002162230 AMERICAN CHEMICAL SOCIETY. WASHINGTON., US ISSN: 0022-2623
S. E. SCHAUS ET AL.: "Practical synthesis of enantiopure cyclic 1,2-amino alcohols via catalytic asymmetric ring opening of meso epoxides" JOURNAL OF ORGANIC CHEMISTRY., vol. 62, no. 12, 1997, pages 4197-4199, XP002162231 AMERICAN CHEMICAL SOCIETY. EASTON., US ISSN: 0022-3263
KIGUCHI ET AL.: "Radical cyclizaton in heterocyce synthesis. Part 9 : A novel synthesis of aminocyclitols and related compounds via stannyl radical cyclization of oxime ethers derived from sugars" TETRAHEDRON., vol. 56, 2000, pages 5819-5833, XP002162232 ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM., NL ISSN: 0040-4020
Attorney, Agent or Firm:
Bryant, Tracey (Alderley Park Macclesfield, Cheshire SK10 4GR, GB)
Global Intellectual, Property Patents (AstraZeneca AB Södertälje, SE)
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Claims:
Claims
1. A compound of formula (I): wherein: R'is H, CH2R5 or COR6; R2 is alky ! Ci. 6 or alkenyl C16, optionally substituted by one or more groups selected from alkyl Cl 6, halogen; R3 is cycloalkyl C38, optionally substituted by R7; R4 is H or alkyl C16, optionally substituted by one or more halogens; R 5 is H, phenyl or alkyl CI6, optionally substituted by halogen, OR8, phenyl; R6 is OR9 or alkyl C16, optionally substituted by one or more groups selected from halogen, OR10, phenyl; R is phenyl, optionally substituted by one or more groups selected from alkyl C16, halogen, oR8; , R9 and Rl°, are independently H or alkyl C16, optionally substituted by one or more groups selected from halogen or alkyl C16 ; or a pharmaceutically acceptable salt or solvate thereof, or a solvate of such a salt.
2. A compound according to claim 1 which is: where R', R2, R3 and R4 are as defined in claim 1.
3. A compound according to claim 2 in which R3 is where R is as defined in claim 1.
4. A compound according to any one of claims 1 to 3 in which R'is H, CH2Ph, CH2CH2OH, or CO2tBu.
5. A compound according to any one of claims 1 to 4 in which R2 is nPr.
6. A compound according to any one of claims 1 to 5 in which R3 is cycloalkyl C38 substituted by phenyl.
7. A compound according to any one of claims 1 to 6 in which R4 is H or methyl.
8. A compound according to claim 1 which is: [3R[3α,4ß(1R*,2S*)]]4[7[(2Phenylcyclopropyl)amino]5(propylthio)3H [1,2,3] triazolo [4,5dlpyrimidin3yl]3pyrrolidinol; [3S[3α,4ß(1S*,2R*)]]3Hydroxy4[7[(2phenylcyclopropyl)amino]5(propylthio)3H [1,2,3] triazolo [4,5dlpyrimidin3yl]1pyrrolidinecarboxylate, 1,1dimethylethyl ester; <BR> <BR> <BR> <BR> <BR> <BR> [3S [3a, 4 (1R*, 2S*)]]3Hydroxy4 [7 [ (2phenylcyclopropyl) amino]5 (propylthio)3H [1,2,3] triazolo [4,5dlpyrimidin3yl]1pyrrolidinecarboxylate, 1,1dimethylethyl ester; [3S[3α,4ß(1S*,2R*)]]4[7[(2Phenylcyclopropyl)amino]5(propylthio)3H [1,2,3] triazolo [4,5dlpyrimidin3yl]3pyrrolidinol; <BR> <BR> <BR> <BR> <BR> <BR> [3R [3 (x, 4p (IR*, 2S*)]] 4 [7 [NMethylN (2phenylcyclopropyl) amino]5 (propylthio) 3H [1,2,3] triazolo [4,5d] pyrimidin3yl]3pyrrolidinol; [3R [3a, 4p (lR*, 2S*)]]1Hydroxyethyl4 [7 [ (2phenylcyclopropyl) amino]5 (propylthio)3H [1,2,3] triazolo [4,5d] pyrimidin3yl]3pyrrolidinol; [3R [3a, 4p (lR*, 2S*)]]4 [7 [ (2Phenylcyclopropyl) amino]5 (propylthio)3H [1,2,3] triazolo [4,5d] pyrimidin3yl]1 (phenylmethyl)3pyrrolidinol; <BR> <BR> <BR> <BR> <BR> <BR> [3R[3α,4ß(1R*,2S*)]]1Acetyl4[7[(2phenyl;cyclopropyl)amino]5(propylthio)3H [1,2,3] triazolo [4, 5d]pyrimidin3yl]3pyrrolidinol. Or a pharmaceutically acceptable salt or solvate thereof, or a solvate of such a salt.
9. A pharmaceutical composition comprising a compound according to any one of claims 1 to 8 in combination with a pharmaceutically acceptable diluent, adjuvent or carrier.
10. A pharmaceutical composition for use in the treatment or prevention of myocardial infarction, thrombotic stroke, transient ischaemic attacks, and/or peripheral vascular disease, comprising a compound according to any one of claims 1 to 8.
11. A pharmaceutical composition for use in the treatment or prevention of unstable or stable angina, comprising a compound according to any one of claims 1 to 8.
12. A compound according to any one of claims 1 to 8 for use in therapy.
13. A compound according to any one of claims 1 to 8 for use in the treatment or prevention of myocardial infarction, thrombotic stroke, transient ischaemic attacks, and/or peripheral vascular disease.
14. A compound according to any one of claims 1 to 8 for use in the treatment or prevention of unstable or stable angina.
15. The use of a compound according to any one of claims 1 to 8 as an active ingredient in the manufacture of a medicament for use in the treatment or prevention of myocardial infarction, thrombotic stroke, transient ischaemic attacks, and/or peripheral vascular disease.
16. The use of a compound according to any one of claims 1 to 8 as an active ingredient in the manufacture of a medicament for use in the treatment or prevention of unstable or stable angina.
17. A method of treatment or prevention of a platelet aggregation disorder which comprises administering to a person suffering from or susceptible to such a disorder a therapeutically effective amount of compound according to any one of claims 1 to 8.
18. A method of treatment or prevention of myocardial infarction, thrombotic stroke, transient ischaemic attacks, and/or peripheral vascular disease, which comprises administering to a person suffering from or susceptible to such a disorder a therapeutically effective amount of a compound according to any one of claims 1 to 8.
19. A method of treatment or prevention of unstable or stable angina, which comprises administering to a person suffering from or susceptible to such a disorder a therapeutically effective amount of a compound according to any one of claims 1 to 8.
20. A process for the preparation of a compound of formula (I), where R'is H, which comprises reacting a compound of formula (II): wherein R2 is as defined in claim 1 and P is a protecting group, with R3R4NH, wherein R3 and R4 are as defined in claim 1, and a base and optionally thereafter removing any protecting groups.
21. Compounds of formula (II), (in), (IV) and (V): wherein R2 is as defined in claim 1 and P is a protecting group.
Description:
NOVEL COMPOUNDS FIELD OF THE INVENTION The present invention provides novel hydroxypyrrolidine compounds, their use as medicaments, compositions containing them and processes for their preparation.

BACKGROUND OF THE INVENTION Platelet adhesion and aggregation are initiating events in arterial thrombosis. Although the process of platelet adhesion to the sub-endothelial surface may have an important role to play in the repair of damaged vessel walls, the platelet aggregation that this initiates can precipitate acute thrombotic occlusion of vital vascular beds, leading to events with high morbidity such as myocardial infarction and unstable angina. The success of interventions used to prevent or alleviate these conditions, such as thrombolysis and platelet-mediated occlusion or re-occlusion also compromises angioplasty.

A number of converging pathways lead to platelet aggregation. Whatever the initial stimulus, the final common event is a cross-linking of platelets by binding of fibrinogen to a membrane-binding site, glycoprotein IIb/IIIa (GPIIb/IIIa). The high anti-platelet efficacy of antibodies or antagonists for GPIIb/IIIa is explained by their interference with this final common event. However, this efficacy may also explain the bleeding problems that have been observed with this class of agent. Thrombin can produce platelet aggregation largely independently of other pathways but substantial quantities of thrombin are unlikely to be present without prior activation of platelets by other mechanisms. Thrombin inhibitors such as hirudin are highly effective anti-thrombotic agents, but again may produce excessive bleeding because they function as both anti-platelet and anti-coagulant agents (The TIMI 9a Investigators (1994), Circulation 90, pp. 1624-1630; The Global Use of Strategies to Open Occluded Coronary Arteries (GUSTO) IIa Investigators (1994) Circulation 90, pp. 1631-1637; Neuhaus K. L. et. al. (1994) Circulation 90, pp. 1638- 1642).

It has been found that ADP acts as a key mediator of thrombosis. ADP-induced platelet aggregation is mediated by the P2T receptor subtype located on the platelet membrane. The P2T receptor (also known as P2YADP or P2TAC) is primarily involved in mediating platelet aggregation/activation and is a G-protein coupled receptor. The pharmacological characteristics of this receptor have been described, for example, in the references by Humphries et al., Br. J. Pharmacology, (1994), 113,1057-1063, and Fagura et al., Br. J.

Pharmacology (1998) 124,157-164. Recently it has been shown that antagonists at this receptor offer significant improvements over other anti-thrombotic agents (see J. Med.

Chem. (1999) 42,213). There is a need to find P2T (P2YADP or P2TAC) antagonists as anti- thrombotic agents.

DESCRIPTION OF THE INVENTION In a first aspect the invention provides a compound of formula (I): wherein: R'is H, CH2R5 or COR6; R2 is alkyl C1-6 or alkenyl C, _6, optionally substituted by one or more groups selected from alkyl Cs 6, halogen; R3 is cycloalkyl C3-8, optionally substituted by R7; R4 is H or alkyl C, 6, optionally substituted by one or more halogens;

R is H, phenyl or alkyl Cl 6, optionally substituted by halogen, OR, phenyl; R6 is OR9 or alkyl C16, optionally substituted by one or more groups selected from halogen, OR10, phenyl; R7 is phenyl, optionally substituted by one or more groups selected from alkyl C1-6, halogen, oR8; R8, R9 and Rl°, are independently H or alkyl C1-6, optionally substituted by one or more groups selected from halogen or alkyl C1-6 ; or a pharmaceutically acceptable salt or solvate thereof, or a solvate of such a salt.

Preferably the compound of formula (I) has the following stereochemistry: Where is the stereochemistry is preferably Preferably R1is H, orCO2tBu.CH2CH2OH, Preferably R2 is n-Pr.

Preferably R3 is cycloalkyl C3-8 substituted by phenyl.

Preferably R4 is H or methyl.

Compounds of the invention include: [3R- [3a, 43 (lR*, 2S*)]]-4- [7- [ (2-Phenylcyclopropyl) amino]-5- (propylthio)-3H- [1,2,3] triazolo [4, 5-d]pyrimidin-3-yl]-3-pyrrolidinol ; <BR> <BR> <BR> <BR> <BR> [3S-[3α,4ß(1S*,2R*)]]-3-Hydroxy-4-[7-[(2-phenylcyclopropyl )amino]-5-(propylthio)-3H- [1,2,3] triazolo [4, 5-d]pyrimidin-3-yl]-1-pyrrolidinecarboxylate, 1,1-dimethylethyl ester; <BR> <BR> <BR> <BR> <BR> [3S-[3α,4ß(1R*,2S*)]]-3-Hydroxy-4-[7-[(2-phenylcyclopropyl )amino]-5-(propylthio)-3H- [1,2,3] triazolo [4, 5-d]pyrimidin-3-yl]-1-pyrrolidinecarboxylate, 1,1-dimethylethyl ester; [3S- [3a, 4 1 S*, 2R*)]]-4- [7- [ (2-Phenylcyclopropyl) amino]-5- (propylthio)-3H- [1,2,3] triazolo [4,5-d] pyrimidin-3-yl]-3-pyrrolidinol; <BR> <BR> <BR> <BR> <BR> [3R- [3a, 4 3 (1R*, 2S*)]]-4- [7- [N-Methyl-N (2-phenylcyclopropyl) amino]-5- (propylthio)- 3H- [1,2,3] triazolo[4,5-d] pyrimidin-3-yl]-3-pyrrolidinol; [3R- [3a, 4 (IR*, 2S*)]]-1-Hydroxyethyl-4- [7- [ (2-phenylcyclopropyl) amino]-5- (propylthio)-3H- [1,2,3] triazolo [4,5-d] pyrimidin-3-yl]-3-pyrrolidinol; [3R- [3a, 4P (IR*, 2S*)]]-4- [7- [ (2-Phenylcyclopropyl) amino]-5- (propylthio)-3H- [1,2,3] triazolo [4,5-dlpyrimidin-3-yl]-1-(phenylmethyl)-3-pyrrolidinol; <BR> <BR> <BR> <BR> <BR> [3R-[3α,4ß(1R*,2S*)]]-1-Acetyl-4-[7-[(2-phenylcyclopropyl) amino]-5-(propylthio)-3H- [1,2,3] triazolo [4,5-dlpyrimidin-3-yl]-3-pyrrolidinol. or a pharmaceutically acceptable salt or solvate thereof, or a solvate of such a salt.

The invention further provides a process for the preparation of a compound of formula (I) which comprises: a. For compounds of formula (I) where R'is H, reacting a compound of formula (II):

wherein R2 is as defined above and P is a protecting group, preferably t-BuOCO, with R3R4NH, wherein R3 and R4 are as defined in (I), and a base, preferably triethylamine or N, N-diisopropylethylamine, in the presence of an inert solvent preferably acetonitrile, preferably at a temperature between about 20 °C and about 100°C and optionally thereafter removing any protecting groups.

Examples of protecting groups include t-BuOCO and CH2Ph. Protecting groups can be added and removed using known reaction conditions. The use of protecting groups is fully described in'Protective Groups in Organic Chemistry', edited by J W F McOmie, Plenum Press (1973), and'Protective Groups in Organic Synthesis', 2nd edition, T W Greene & P G M Wutz, Wiley-Interscience (1991).

A compound of formula (II) can be prepared by diazotizing a compound of formula (m):

where R2 and P are defined above, and where necessary other reactive groups might also be protected, with a C 1-6 alkyl nitrite, preferably iso-amylnitrite in the presence of an inert solvent preferably acetonitrile at a temperature of between about 20 and about 80°C, or with an alkali metal nitrite, preferably sodium nitrite, under aqueous acidic conditions, preferably aqueous hydrochloric or acetic acid and preferably at a temperature between about 0°C and about 20°C.

A compound of formula (III) can be prepared by reacting a compound of formula (IV):

wherein P is a protecting group, with a compound of formula (V):

wherein R is as defined in formula (I) and is preferably n-propyl. The reaction is carried out in the presence of a base, preferably triethylamine or N, N-diisopropylethylamine, in an inert solvent preferably N, N-dimethylformamide or n-butanol, at a temperature between about 100°C and about 150°C.

The preparation of the formula (IV) racemate is described in Okada et al., Chem. Pharm Bull. (1993), 41,132-8; the preparation of formula (IV) enantiomers is described in Schaus, et al., J. Org. Chem. (1997), 62,4197-9; the preparation of a compound of formula V (R is n-propyl) is described in EP 508687.

Compounds of formula (I) where R2 is other than n-propyl are prepared by displacement of the sulphone group from a compound of formula (VI):

where R2 is n-propyl, P, R3 and R4 are defined above, using either a sodium alkylthiolate (R2SNa) in the presence of an inert solvent, preferably N, N-dimethylformamide, preferably at a temperature between about 0°C and about 50°C or sodium hydrosulphide (NaSH), in the presence of an inert solvent preferably N, N-dimethylformamide. The latter reaction is followed by alkylation with an alkyl halide (R2X, where X is a leaving group preferably bromide or iodide), preferably at a temperature between about 0°C and about 50°C and optionally thereafter removing any protecting groups.

The preparation of the compound of formula (VI), where R2 is n-propyl, is preferably carried out by reacting a compound of formula (I), where R'has been protected as described above, with a peracid, preferably m-chloroperbenzoic acid, in the presence of an inert chlorocarbon solvent such as dichloromethane or a mixture of dichloromethane and methanol, at a temperature between about 0 °C and about 50 °C. b. For compounds of formula (1) where Rl is CH2R5, where R is defined in formula (I), the reaction scheme outlined in a. above is followed by reductive amination using an aldehyde (RSCHO) and a reducing agent, preferably sodium triacetoxyborohydride, and optionally thereafter removing any protecting groups. The reductive amination reaction is preferably carried out in the presence of an inert solvent preferably N, N-dimethylformamide, tetrahydrofuran or a mixture of acetonitrile and N-methylpyrrolidone and preferably at a temperature between about 0 °C and about 50 °C. c. For compounds of formula (I) where R'is COR, where R6 is defined in formula (I), the reaction scheme outlined in a. above is followed by acylation using an acid halide (R6COX) or anhydride ( (R6CO) 20) or an acid (R6CO2H) in the presence of a suitable activating agent preferably N, N'-carbonyldiimidazole or N, N'-dicyclohexylcarbodiimide, and a base preferably triethylamine or N, N-diisopropylethylamine, and optionally thereafter removing any protecting groups. The acylation is preferably carried out in the presence of an inert solvent preferably dichloromethane, chloroform or tetrahydrofuran and preferably at a temperature between about 0 °C and about 50 °C.

Compounds of formula (II), (E), (IV) and (V) form a further aspect of the invention.

Salts of the compounds of formula (I) may be formed by reacting the free base, or a salt or a derivative thereof, with one or more equivalents of the appropriate acid (for example a hydrohalic (especially HCI), sulphuric, oxalic or phosphoric acid). The reaction may be carried out in a solvent or medium in which the salt is insoluble or in a solvent in which the salt is soluble, e. g. water, ethanol, tetrahydrofuran, or diethyl ether, which may be removed in vacuo, or by freeze drying. The reaction may also be a metathetical process or it may be carried out on an ion exchange resin. The non-toxic physiologically acceptable salts are preferred, although other salts may be useful, e. g. in isolating or purifying the product.

The compounds of the invention act as P2r (P2YADP or P2TAC) receptor antagonists.

Accordingly, the compounds are useful in therapy, including combination therapy, particularly they are indicated for use as: inhibitors of platelet activation, aggregation and degranulation, promoters of platelet disaggregation, anti-thrombotic agents or in the treatment or prophylaxis of unstable angina, coronary revascularisation procedures including angioplasty (PTCA), myocardial infarction, perithrombolysis, primary arterial thrombotic complications of atherosclerosis such as thrombotic or embolic stroke, transient ischaemic attacks, peripheral vascular disease, myocardial infarction with or without thrombolysis, arterial complications due to interventions in atherosclerotic disease such as angioplasty, endarterectomy, stent placement, coronary and other vascular graft surgery, thrombotic complications of surgical or mechanical damage such as tissue salvage following accidental or surgical trauma, reconstructive surgery including skin and muscle flaps, conditions with a diffuse thrombotic/platelet consumption component such as disseminated intravascular coagulation, thrombotic thrombocytopaenic purpura, haemolytic uraemic syndrome, thrombotic complications of septicaemia, adult respiratory distress syndrome, anti-phospholipid syndrome, heparin-induced thrombocytopaenia and pre-eclampsia/eclampsia, or venous thrombosis such as deep vein thrombosis, venoocclusive disease, haematological conditions such as myeloproliferative disease, including thrombocythaemia, sickle cell disease; or in the prevention of mechanically- induced platelet activation in vivo, such as cardio-pulmonary bypass and extracorporeal membrane oxygenation (prevention of microthromboembolism), mechanically-induced

platelet activation in vitro, such as use in the preservation of blood products, e. g. platelet concentrates, or shunt occlusion such as in renal dialysis and plasmapheresis, thrombosis secondary to vascular damage/inflammation such as vasculitis, arteritis, glomerulonephritis, inflammatory bowel disease and organ graft rejection, conditions such as migraine, Raynaud's phenomenon, conditions in which platelets can contribute to the underlying inflammatory disease process in the vascular wall such as atheromatous plaque formation/progression, stenosis/restenosis and in other inflammatory conditions such as asthma, in which platelets and platelet-derived factors are implicated in the immunological disease process. Further indications include treatment of CNS disorders and prevention of the growth and spread of tumours.

In particular, the compounds of the invention are useful in the treatment or prevention of myocardial infarction, thrombotic stroke, transient ischaemic attacks, peripheral vascular disease and stable and unstable angina, especially unstable angina.

The invention also provides a method of treatment or prevention of the above disorders which comprises administering to a patient suffering from or susceptible to such a disorder a therapeutically effective amount of a compound according to the invention.

According to the invention there is further provided the use of a compound according to the invention as an active ingredient in the manufacture of a medicament for use in the treatment or prevention of the above disorders.

The compounds may be administered topically, e. g. to the lung and/or the airways, in the form of solutions, suspensions, HFA aerosols and dry powder formulations; or systemically, e. g. by oral administration in the form of tablets, pills, capsules, syrups, powders or granules, or by parenteral administration in the form of sterile parenteral solutions or suspensions, by subcutaneous administration, or by rectal administration in the form of suppositories or transdermally.

The compounds of the invention may be administered on their own or as a pharmaceutical composition comprising the compound of the invention in combination with a

pharmaceutically acceptable diluent, adjuvant or carrier. Particularly preferred are compositions not containing material capable of causing an adverse, e. g. an allergic, reaction.

Dry powder formulations and pressurised HFA aerosols of the compounds of the invention may be administered by oral or nasal inhalation. For inhalation the compound is desirably finely divided. The compounds of the invention may also be administered by means of a dry powder inhaler. The inhaler may be a single or a multi dose inhaler, and may be a breath actuated dry powder inhaler. One possibility is to mix the finely divided compound with a carrier substance, e. g. a mono-, di-or polysaccharide, a sugar alcohol or another polyol. Suitable carriers include sugars and starch. Alternatively the finely divided compound may be coated by another substance. The powder mixture may also be dispensed into hard gelatine capsules, each containing the desired dose of the active compound. Another possibility is to process the finely divided powder into spheres which break up during the inhalation procedure. This spheronized powder may be filled into the drug reservoir of a multidose inhaler, e. g. that known as the Turbuhaler in which a dosing unit meters the desired dose which is then inhaled by the patient. With this system the active compound with or without a carrier substance is delivered to the patient.

The pharmaceutical composition comprising the compound of the invention may conveniently be tablets, pills, capsules, syrups, powders or granules for oral administration; sterile parenteral or subcutaneous solutions, suspensions for parenteral administration or suppositories for rectal administration.

For oral administration the active compound may be admixed with an adjuvant or a carrier, e. g. lactose, saccharose, sorbitol, mannitol, starches such as potato starch, corn starch or amylopectin, cellulose derivatives, a binder such as gelatine or polyvinylpyrrolidone, and a lubricant such as magnesium stearate, calcium stearate, polyethylene glycol, waxes, paraffin, and the like, and then compressed into tablets. If coated tablets are required, the cores, prepared as described above, may be coated with a concentrated sugar solution, which may contain e. g. gum arabic, gelatine, talcum, titanium dioxide, and the like.

Alternatively, the tablet may be coated with a suitable polymer dissolved either in a readily volatile organic solvent or an aqueous solvent.

For the preparation of soft gelatine capsules, the compound may be admixed with e. g. a vegetable oil or polyethylene glycol. Hard gelatine capsules may contain granules of the compound using either the above mentioned excipients for tablets, e. g. lactose, saccharose, sorbitol, mannitol ; starches, cellulose derivatives or gelatine. Also liquid or semisolid formulations of the drug may be filled into hard gelatine capsules.

Liquid preparations for oral application may be in the form of syrups or suspensions, for example solutions containing the compound, the balance being sugar and a mixture of ethanol, water, glycerol and propylene glycol. Optionally such liquid preparations may contain colouring agents, flavouring agents, saccharine and carboxymethylcellulose as a thickening agent or other excipients known to those skilled in art.

EXAMPLES The invention is illustrated by the following non-limiting examples.

In the examples the NMR spectra were measured on a Varian Unity Inova 300 or 400 spectrometer and the MS spectra were measured as follows: EI spectra were obtained on a VG 70-250S or Finnigan Mat Incos-XL spectrometer, FAB spectra were obtained on a VG70-250SEQ spectrometer, ESI and APCI spectra were obtained on Finnigan Mat SSQ7000 or a Micromass Platform spectrometer. Preparative HPLC separations were generally performed using a Novapak, Bondapake or Hypersil column packed with BDSC-18 reverse phase silica. Flash chromatography (indicated in the Examples as (Si02)) was carried out using Fisher Matrix silica, 35-70 , m. For examples which show the presence of rotamers in the proton NMR spectra only the chemical shifts of the major rotamer are quoted.

Example 1

[3R-[3α,4ß(1R*,2S*)]]-4-[7-[(2-Phenylcyclopropyl)amino]-5- (propylthio)-3H- [1,2,3] triazolo [4,5-d] pyrimidin-3-yl]-3-pyrrolidinol, trifluoroacetate salt a) (3R,4R)-3-[[5-Amino-6-chloro-2-(propylthio)pyrimidin-4-yl]am ino]-4-hydropxy-1- pyrrolidinecarboxylate, 1,1-dimethylethyl ester Triethylamine (18.8ml) was added to a solution of (3R, 4R)-4-amino-3-hydroxy-1- pyrrolidinecarboxylate, 1,1-dimethylethyl ester (prepared as described in J. Org. Chem., using the (SS) (salen) Cr (III) complex) (3.63g) and 4,6-dichloro-2- propylthiopyrimidine-5-amine (prepared as described in EP508687) (3.56g) and the resulting mixture was heated at 100°C for 24 hours. The excess triethylamine was removed in vacuo and the residue was diluted with water and extracted with ethyl acetate. The organic phase was washed with brine, dried and concentrated in vacuo. The residue was purified by chromatography (SiO2, dichloromethane: methanol, 97: 3 as eluant) followed by trituration with diethylether/iso-hexane to give the subtitle compound (4.16g).

MS (APCI) 404 (M+H+, 100%). b) (3R, 4R)-4- [7-Chloro-5- (propylthio)-3H- [1,2,3] triazolo [4, 5-d] pyrimidin-3-yl]-3- hydroxy-1-pyrrolidinecarboxylate, 1,1-dimethylethyl ester The product from step a) (4.1 g) and iso-amylnitrite (2.74ml) were heated under reflux in acetonitrile (20ml) for 1 hour. The reaction mixture was concentrated in vacuo and the residue purified by chromatography (Si02, ethyl acetate: iso-hexane, 1: 4 as eluant) to afford the sub-title compound (3.32g).

MS (APCI) 415 (M+H+, 100%). c) [3R- [3a, 4 (3 (1R*, 2S*)]]-3-Hydroxy-4- [7- [ (2-phenylcyclopropyl) amino]-5- (propylthio)-3H- [1,2,3] triazolo [4,5-dlpyrimidin-3-yl]-1-pyrrolidinecarboxylate, 1,1- dimethylethyl ester

N, N-diisopropylethylamine (3ml) was added to a solution of the product from step b) (1.2g) and (lR-trans)-2-phenylcyclopropanamine, [R-(R*, R*)]-2,3-dihydroxybutanedioate (1: 1) (prepared as described by L. A. Mitscher et al., J. Med. Chem., 1986,29,2044) (1.23g) in dichloromethane (40ml). The reaction mixture was stirred at room temperature for 16 hours then washed with water. The organic phase was washed with dilute hydrochloric acid and brine, dried and concentrated in vacuo. The residue was purified by chromatography (Si02, dichloromethane: methanol, 99: 1 as eluant) to afford the sub-title compound (1.12g).

MS (APCI) 512 (M+H+, 100%). d) [3R-[3α,4ß(1R*,2S*)]]-4-[7-[(2-Phenylcyclopropyl)amino]-5- (propylthio)-3H- [1,2,3] triazolo [4, 5-d]pyrimidin-3-yl]-3-pyrrolidinol, trifluoroacetate salt The product from step c) (0.54g) was dissolved in trifluoroacetic acid (22.5ml) and water (2.5ml) and the solution stirred at room temperature for 4h. The solvents were evaporated and the residue dried by azeotropic distillation with toluene (4x50m1) followed by methanol (50ml) to give a yellow foam. The crude product was triturated with diethylether (50ml) to afford a white powder that was recrystallised (ethyl acetate) to afford the title compound (0.37g) as a white solid.

MS (APCI) 412 (M+H+, 100%) NMR 8H (d6-DMSO) 9.5 (2H, br s), 9.47 (1H, d), 7.10-7.35 (5H, m), 6.28 (1H, d), 5.26 (1H, br m), 4.65 (1H, br s), 3.90 (2H, m), 3.52 (1H, d, AB), 3.3 (1H, m), 3.24. (1H, m), 2.8- 3.0 (2H, t, AB), 2.13 (1H, m), 1.54 (1H, d, t), 1.47 (2H, sext.), 1.34 (1H, br q), 0.79 (3H, t).

Example 2 <BR> <BR> <BR> <BR> <BR> <BR> [3S- [3a, 4 (3 (1S*, 2R*)]]-3-Hydroxy-4- [7- [ (2-phenylcyclopropyl) amino]-5- (propylthio)- 3H- [1,2,3] triazolo [4, 5-d] pyrimidin-3-yl]-l-pyrrolidinecarboxylate, 1,1-dimethylethyl ester

a) (3S,4S)-3-[[5-Amino-6-chloro-2-(propylthio)pyrimidin-4-yl]am ino]-4-hydroxy-1- pyrrolidinecarboxylate, 1,1-dimethylethyl ester Prepared according to the method of Example 1, step a) using (3S, 4S)-4-amino-3-hydroxy- 1-pyrrolidinecarboxylate, 1,1-dimethylethyl ester (prepared as described in J. Org. Chem., 1997,62,4197 using a (R, R) (salen) Cr (III) complex).

MS (APCI) 404/406 (M+H+), 404 (100%). b) (3S, 4S)-4- [7-Chloro-5- (propylthio)-3H- [1,2,3] triazolo [4,5-d] pyrimidin-3-yl]-3- hydroxy-1-pyrrolidinecarboxylate, 1,1-dimethylethyl ester Prepared according to the method of Example 1, step b).

MS (APCI) 315 (M+H-BOC+, 100%). c) [3S-[3α,4ß(1S*,2R*)]]-3-Hydroxy-4-[7-[(2-phenylcyclopropyl )amino]-5- (propylthio)-3H- [1,2,3] triazolo [4,5-d] dlpyrimidin-3-yl]-1-pyrrolidinecarboxylate, 1,1- dimethylethyl ester Prepared according to the method of Example 1, step c).

MS (APCI) 512 (M+H+, 100%).

NMR 8H (d6-DMSO) 9.40 (1H, d), 7.31-7.27 (2H, m), 7.20-7.15 (3H, m), 5.78-5.76 (1H, m), 5.11-5.06 (1H, m), 4.61-4.56 (1H, m), 3.94-3.81 (2H, m), 3.69-3.62 (1H, m), 3.30-3.18 (2H, m), 3.11-2.80 (2H, m), 2.15-2.10 (1H, m), 1.73-1.23 (13H, m), 0.80 (3H, t).

Example 3

[3S- [3a, 4 (1R*, 2S*)]]-3-Hydroxy-4- [7- [ (2-phenylcyclopropyl) amino]-5- (propylthio)- 3H- [1,2,3] triazolo [4,5-d] pyrimidin-3-yl]-1-pyrrolidinecarboxylate, 1, 1-dimethylethyl ester a) [3S-[3α,4ß(1R*,2S*)]]-3-Hydroxy-4-[7-[(2-phenylcyclopropyl )amino]-5- (propylthio)-3H- [1,2,3] triazolo [4,5 dlpyrimidin-3-yl]-1-pyrrolidinecarboxylate, 1,1- dimethylethyl ester Prepared according to the method of Example 2, step c) using (lS-trans)-2-phenyl- cyclopropanamine, [S- (R *, R *)]-2,3-dihydroxybutanedioate (1: 1) (prepared as described by L. A. Mitscher et al., J. Med. Chem., 1986,29,2044).

MS (APCI) 512 (M+H+, 100%).

NMR 8H (d6-DMSO) 9.40 (1H, d), 7.31-7.27 (2H, m), 7.20-7.15 (3H, m), 5.78-5.76 (1H, m), 5.11-5.06 (1H, m), 4.62-4.58 (1H, m), 3.94-3.81 (2H, m), 3.69-3.63 (1H, m), 3.30-3.18 (2H, m), 3.11-2.80 (2H, m), 2.15-2.11 (1H, m), 1.72-1.23 (13H, m), 0.80 (3H, t).

Example 4 [3S-[3α,4ß-(1S*,2R*)]]-4-[7-[(2-Phenylcyclopropyl)amino]-5 -(propylthio)-3H- [1,2,3] triazolo [4,5-d] pyrimidin-3-yl]-3-pyrrolidinol, trifluoroacetate salt a) [3S-[3α,4ß(1S*,2R*)]]-4-[7-[(2-Phenylcyclopropyl)amino]-5- (propylthio)-3H- [1,2,3] triazolo [4,5-d] pyrimidin-3-yl]-3-pyrrolidinol, trifluoroacetate salt Prepared according to the method of Example 1, step d) using the compound of Example 2, step c) MS (APCI) 412 (M+H+, 100%)

NMR 8H (d6-DMSO) 9.5 (2H, br s), 9.48 (1H, d), 7.10-7.35 (5H, m), 6.30 (1H, d), 5.26 (1H, br m), 4.64 (1H, br s), 3.9 (2H, m), 3.5 (1H, d, AB), 3.26 (1H, m), 3.24. (1H, m), 2.7- 3.0 (2H, t, AB), 2.11 (1H, m), 1.55 (1H, d, t), 1.46 (2H, sext.), 1.34 (1H, br q), 0.78 (3H, t).

Example 5 [3R- [3a, 4 (3 (lR*, 2S*)]]-4- [7- [N-Methyl-N- (2-phenylcyclopropyl) amino]-5- (propylthio)-3H- [1,2,3] triazolo [4,5-d] pyrimidin-3-yl]-3-pyrrolidinol, trifluoroacetate salt a) [3R- [3a, 4 (3 (1R*, 2S*)]]-3-Hydroxy-4- [7- [N-methyl-N- (2-phenylcyclopropyl) amino]- 5- (propylthio)-3H- [1,2,3] triazolo [4,5-d] pyrimidin-3-yl]-1-pyrrolidinecarboxylate, 1, 1- dimethylethyl ester.

N, N-diisopropylethylamine (0.5ml) was added to a solution of the product from Example 1 step b) (0.3g) and (lR-trans)-N-methyl-2-phenylcyclopropylamine hydrochloride (prepared as described by C. Kaiser et al, J. Org. Chem., 1962,27,768-773, using (1R- trans)-2-phenylcyclopropanamine, [R-(R*,R*)]-2, 3-dihydroxybutanedioate (1: 1) (prepared as described by L. A. Mitscher et al, J. Med. Chem., 1986,29,2044) (0.2g) in dichloromethane (20ml). The reaction mixture was stirred at room temperature for 48 hours then washed with water. The organic phase was washed with dilute hydrochloric acid and brine, dried and concentrated in vacuo. The residue was purified by chromatography (Si02, dichloromethane: methanol, 99: 1 as eluant) to afford the sub-title compound (0.36g).

MS (APCI) 470 (M+H+, 100%). b) [3R- [3a, 4 (3 (1R*, 2S*)]]-4- [7- [N-Methyl-N- (2-phenylcyclopropyl) amino]-5- (propylthio)-3H- [1,2,3] triazolo [4, 5-d]pyrimidin-3-yl]-3-pyrrolidinol, trifluoroacetate salt

A solution of the product from step a) (0.36g) in 9: 1 trifluoroacetic acid: water (10ml) was stirred at room temperature for 2 hours. The solvent was removed and co-evaporated with toluene (3x). The residue was dissolved in water (20ml) and ethanol (1 ml) and freeze-dried for 16 hours to give the title compound (0.33g).

MS (APCI) 426 (M+H+, 100%).

NMR 8H (d6-DMSO) 9.33 (2H, br s), 7.29 (2H, m), 7.20 (3H, m), 6.04 (1H, br s), 5.27 (1H, m), 4.72 (1H, d), 3.84-3.97 (2H, m), 3.56 (4H, m), 3.31 (1H, d), 3.06 (3H, under DMSO), 2.43 (1H, under H2O), 1.54-1.66 (3H, m), 1.45 (1H, m), 0.94 (3H, t).

Example 6 [3R- [3c, 4 (1R*, 2S*)]]-1-Hydroxyethyl-4- [7- [ (2-phenylcyclopropyl) amino]-5- (propylthio)-3H- [1,2,3] triazolo [4,5-d] pyrimidin-3-yl]-3-pyrrolidinol, trifluoroacetate salt a) [3R- [3a, 4 (3 (1R*, 2S*)]]-1- [2- [ (1, 1-Dimethylethyl) (dimethyl) silyl] oxy] ethyl-4- [7- [ (2- phenylcyclopropyl) amino]-5- (propylthio)-3H- [1,2,3] triazolo [4,5-d] pyrimidin-3-yl]-3- pyrrolidinol.

[ [ (l, l-Dimethylethyl) dimethylsilyl] oxy] acetaldehyde (Tet. Lett., (0.27g) was added to a solution of the product from Example 1 step d) (0.4g) and sodium triacetoxyborohydride (0.48g) in dry tetrahydrofuran (10ml) and the mixture was stirred at room temperature for 16 hours. The reaction mixture was diluted with water and extracted with ethyl acetate (thrice). The combined organic phase was washed with brine, dried and concentrated in vacuo. The residue was purified by chromatography (Si02, dichloromethane: methanol, 99: 1 as eluant) to give the sub-title compound (0.2g).

MS (APCI) 570 (M+H+, 100%).

b) [3R- [3a, 4 (1R*, 2S*)]]-1-Hydroxyethyl-4- [7- [ (2-phenylcyclopropyl) amino]-5- (propylthio)-3H- [1,2,3] triazolo [4,5-d] pyrimidin-3-yl]-3-pyrrolidinol, trifluoroacetate salt Tetrabutylammonium fluoride hydrate (0.2g) was added to a solution of the product from step a) (0.2g) in dry tetrahydrofuran (lOml) and the mixture was stirred at room temperature for 16 hours. The solvent was removed in vacuo and the residue was purified by chromatography (Si02, dichloromethane: methanol, 95: 5 as eluant). Trifluoroacetic acid (22F1) was added to a solution of the resulting oil in diethylether (5ml) and the solid formed was collected by filtration to give the title compound (0.12g).

MS (APCI) 456 (M+H+, 100%).

NMR 8H (d6-DMSO+D20) 7.31 (2H, m), 7.21 (3H, m), 5.36 (1H, br s), 4.87 (1H, br s), 4.18 (1H, m), 4.04 (1H, m), 3.82 (3H, m), 3.55 (1H, under H20), 3.45 (2H, m), 3.29 (1H, br s), 3.02 (2H, br s), 2.22 (1H, br s), 1.58 (2H, br s), 1.50 (1H, m), 1.36 (1H, m), 0.88 (3H, br s).

Example 7 [3R- [3a, 4(3 (lR*, 2S*)]]-4- [7- [ (2-Phenylcyclopropyl) amino]-5- (propylthio)-3H- [1,2,3]triazolo[4,5-d]pyrimidin-3-yl]-1-(phenylmethyl)-3-pyr rolidinol,trifluoroacetate salt Benzaldehyde (0. lml) was added to a solution of the product from Example 1 step d) (0.26g) and sodium triacetoxyborohydride (0.32g) in dry tetrahydrofuran (10ml) and the mixture was stirred at room temperature for 3 hours. The reaction mixture was diluted with water and extracted with ethyl acetate (thrice). The combined organic phase was washed with brine, dried and concentrated. Trifluoroacetic acid (201l1) was added to a solution of the resulting oil in diethylether (5ml) and the solvent was removed in vacuo. The residue was dissolved in water (20ml) and ethanol (5ml) and freeze-dried for 16 hours. Purification by chromatography (HPLC, Novapaks C18 column, 0.1% aqueous trifluoroacetic

acid: acetonitrile, gradient elution 75: 25 to 0: 100 over 15 minutes), followed by freeze drying gave the title compound (0.094g).

MS (APCI) 502 (M+H+, 100%).

NMR 8H (d6-DMSO+D20) 7.53 (2H, d), 7.48 (3H, m), 7.31 (2H, m), 7.20 (3H, m), 5.34 (1H, m), 4.88 (1H, m), 4.48 (2H, q), 4.05 (1H, m), 3.90 (1H, m), 3.72 (1H, m), 3.41 (1H, m), 3.30 (1H, br m), 3.01 (2H, br m), 2.21 (1H, br s), 1.50-1.56 (3H, m), 1.36 (1H, m), 0.87 (3H, br s).

Example 8 <BR> <BR> <BR> <BR> <BR> <BR> [3R- [3a, 4 (3 (1R*, 2S*)]]-1-Acetyl-4- [7- [ (2-phenylcyclopropyl) amino]-5- (propylthio)- 3H- [1,2,3] triazolo [4,5 dlpyrimidin-3-yl]-3-pyrrolidinol.

A mixture of the product from Example 1 step d) (0.17g), acetic anhydride (0.046ml) and pyridine (0.078ml) in dichloromethane (3ml) was stirred at room temperature under a nitrogen atmosphere for 16 hours. The reaction mixture was diluted with water and extracted with dichloromethane (twice). The combined organic phase was washed with dilute hydrochloric acid and brine, dried and concentrated in vacuo. The residue was purified by chromatography (Si02, dichloromethane: methanol, 98: 2 as eluant) followed by trituration with acetonitrile to give the title compound (0.06g).

MS (APCI) 454 (M+H+, 100%).

NMR 8H (d6-DMSO) 9.39 (1H, m), 7.30 (2H, m), 7.19 (3H, m), 5.77-5.86 (1H, m), 5.09- 5.16 (1H, m), 4.60-4.69 (1H, m), 4.00-4.13 (1H, m), 3.91 (2H, m), 3.46,3.68 (1H, m), 3.21 (1H, br m), 2.82-2.91 (2H, m), 2.13 (1H, m), 1.98 (3H, d), 1.34-1.54 (4H, m), 0.79 (3H, t).

Pharmacological data The preparation for the assay of the P2T (P2YADP or P2TAc)-receptor agonist/antagonist activity in washed human platelets for the compounds of the invention was carried out as follows.

Human venous blood (100 ml) was divided equally between 3 tubes, each containing 3.2% trisodium citrate (4 ml) as anti-coagulant. The tubes were centrifuged for 15 minutes at 240G to obtain a platelet-rich plasma (PRP) to which 300 ng/ml prostacyclin was added to stabilize the platelets during the washing procedure. Red cell free PRP was obtained by centrifugation for 10 minutes at 125G followed by further centrifugation for 15 minutes at 640G. The supernatant was discarded and the platelet pellet resuspended in modified, Calcium Free Tyrode solution (10 ml) (CFT), composition: NaCI 137mM, NaHCO3 11. 9mM, NaH2PO4 0.4mM, KCl 2.7 mM, MgCl2 1.1 mM, dextrose 5.6 mM, gassed with 95% 02/5% C02 and maintained at 37°C. Following addition of a further 300 ng/ml PGI2, the pooled suspension was centrifuged once more for 15 minutes at 640G. The supernatant was discarded and the platelets resuspended initially in 10 ml CFT with further CFT added to adjust the final platelet count to 2x 105/ml. This final suspension was stored in a 60 ml syringe at 3°C with air excluded. To allow recovery from PGI2-inhibition of normal function, platelets were used in aggregation studies no sooner than 2 hours after final resuspension.

In all studies, 3 ml aliquots of platelet suspension were added to tubes containing CaCl2 solution (60 Rl of 50 mM solution with a final concentration of ImM). Human fibrinogen (Sigma, F 4883) and 8-sulphophenyltheophylline (8-SPT which was used to block any Pl- agonist activity of compounds) were added to give final concentrations of 0.2 mg/ml (60 RI of 10 mg/ml solution of clottable protein in saline) and 300 nM (10 il of 15 mM solution in 6% glucose), respectively. Platelets or buffer as appropriate were added in a volume of 150 RI to the individual wells of a 96 well plate. All measurements were made in triplicate in platelets from each donor.

The agonist/antagonist potency was assessed as follows.

Aggregation responses in 96 well plates were measured using the change in absorbance given by the plate reader at 660 nm. Either a Bio-Tec Ceres 900C or a Dynatech MRX were used as the plate reader.

The absorbance of each well in the plate was read at 660 nm to establish a baseline figure.

Saline or the appropriate solution of test compound was added to each well in a volume of 10 gl to give a final concentration of 1,1,10 or 100 mM. The plate was then shaken for 5 min on an orbital shaker on setting 10 and the absorbance read at 660 nm.

Aggregation at this point was indicative of agonist activity of the test compound. Saline or ADP (30 mM; 10 fol ouf 450 mM) was then added to each well and the plate shaken for a further 5 min before reading the absorbance again at 660 nm.

Antagonist potency was estimated as a % inhibition of the control ADP response to obtain an IC50. Compounds exemplified have pICso values of more than 5.0.