Title:
TRICHOPLUSIA NI PIGGYBAC TRANSPOSASES WITH REDUCED INTEGRATION ACTIVITY
Document Type and Number:
WIPO Patent Application WO/2013/012824
Kind Code:
A3
Abstract:
The present invention is directed to nucleic acid and amino acid sequences of a novel piggyBac transposase enzymes created by modifying the transposase of Trichoplusia ni. The piggyBac transposases of the present invention are functionally active or hyperactive for excision and have decreased integration activity compared to wild type Trichoplusia ni piggyBac transposase enzyme. These transposases are ideal for use in methods of transforming cells and organisms. In particular embodiments, the present invention provides methods of transient integration and expression of transgenes.
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Inventors:
CRAIG NANCY L (US)
Application Number:
PCT/US2012/046976
Publication Date:
April 25, 2013
Filing Date:
July 16, 2012
Export Citation:
Assignee:
UNIV JOHNS HOPKINS (US)
HUGHES HOWARD MED INST (US)
CRAIG NANCY L (US)
HUGHES HOWARD MED INST (US)
CRAIG NANCY L (US)
International Classes:
C12N9/10; C07K14/195; C12N15/54
Domestic Patent References:
| WO2010099301A2 | 2010-09-02 |
Foreign References:
| US20090042297A1 | 2009-02-12 |
Other References:
YUSA, K. ET AL.: "A hyperactive piggyBac transposase for mammalian applications.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 108, no. 4, 25 January 2011 (2011-01-25), pages 1531 - 1536
GALVAN, DL ET AL.: "Genome-wide mapping of PiggyBac transposon integrations in primary human T cells.", JOURNAL OF IMMUNOTHERAPY, vol. 32, no. 8, 1 October 2009 (2009-10-01), pages 837 - 844
GALVAN, DL ET AL.: "Genome-wide mapping of PiggyBac transposon integrations in primary human T cells.", JOURNAL OF IMMUNOTHERAPY, vol. 32, no. 8, 1 October 2009 (2009-10-01), pages 837 - 844
Attorney, Agent or Firm:
KIRCHANSKI, Stefan, J. (2049 Century Park East Suite 210, Los Angeles CA, US)
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