Title:
TRITERPENE OXIDASE DERIVED FROM GLYCHYRRHIZA PLANT, GENE ENCODING THE OXIDASE, AND METHOD FOR UTILIZING THE GENE
Document Type and Number:
WIPO Patent Application WO/2010/024437
Kind Code:
A1
Abstract:
The object aims to identify a protein having an activity to oxidize an oleanane-type triterpene and a gene encoding the protein, and to provide the protein, the gene and use of the protein or the gene. For example, disclosed are a protein having an activity to oxidize an oleanane-type triterpene and produced from a plant belonging to the genus Fabaceae, a gene encoding the protein, and use of the gene. The protein is depicted in, for example, SEQ ID NO:4, SEQ ID NO:14 or SEQ ID NO:18. The gene encoding the protein is depicted in, for example, SEQ ID NO:3, SEQ ID NO:13 or SEQ ID NO:17. A transformant having the gene introduced therein is produced, and a triterpene oxidase is produced by using the transformant.
Inventors:
MURANAKA TOSHIYA (JP)
SEKI HIKARU (JP)
OHYAMA KIYOSHI (JP)
SUDO HIROSHI (JP)
SAWAI SATORU (JP)
SAITO KAZUKI (JP)
SEKI HIKARU (JP)
OHYAMA KIYOSHI (JP)
SUDO HIROSHI (JP)
SAWAI SATORU (JP)
SAITO KAZUKI (JP)
Application Number:
PCT/JP2009/065197
Publication Date:
March 04, 2010
Filing Date:
August 31, 2009
Export Citation:
Assignee:
RIKEN (JP)
TOKIWA PHYTOCHEMICAL CO LTD (JP)
UNIV CHIBA NAT UNIV CORP (JP)
MURANAKA TOSHIYA (JP)
SEKI HIKARU (JP)
OHYAMA KIYOSHI (JP)
SUDO HIROSHI (JP)
SAWAI SATORU (JP)
SAITO KAZUKI (JP)
TOKIWA PHYTOCHEMICAL CO LTD (JP)
UNIV CHIBA NAT UNIV CORP (JP)
MURANAKA TOSHIYA (JP)
SEKI HIKARU (JP)
OHYAMA KIYOSHI (JP)
SUDO HIROSHI (JP)
SAWAI SATORU (JP)
SAITO KAZUKI (JP)
International Classes:
C12N15/00; C12N5/10; C12N9/04; C12N15/09; C12P7/40; C12Q1/68
Domestic Patent References:
| WO2003093425A2 | 2003-11-13 | |||
| WO2005080572A1 | 2005-09-01 |
Foreign References:
| JP2007204769A | 2007-08-16 |
Other References:
LI, L. ET AL.: "Genome-wide identification and characterization of putative cytchrome P450 genes in the model legume Medicago truncatula", PLANTA, vol. 226, 2007, pages 109 - 123, XP019517499
NOMURA T ET AL.: "Cytochrome P450s in plant steroid hormone synthesis and metabolism", PHYTOCHEM.REV., vol. 5, 2006, pages 421 - 432, XP019453412
SHIBUYA, M. ET AL.: "Identification of P-amyrin and sophoradiol 24-hydroxylase by expressed sequence tag mining and functional expression assay", FEBS J., vol. 273, 2006, pages 948 - 959, XP002472147
SEKI, H. ET AL.: "Licorice beta-amyrin 11 oxidase, a cytochrome P450 with a key role in the biosynthesis of the triterpene sweetener glycyrrhizin", PROC.NATL.ACAD.SCI.USA, vol. 105, no. 37, 2008, pages 14204 - 14209, XP008131547
See also references of EP 2336312A4
SHIBUYA ET AL.: "Identification of beta-amyrin and sophoradiol 24-hydroxylase by expressed sequence tag mining and functional expression assay", FEBS J., vol. 273, no. 5, March 2006 (2006-03-01), pages 948 - 59
SAMBROOK, J.: "Molecular Cloning: a Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
"DNA microarray to saishin PCR ho (DNA microarray and the latest PCR method)", 2000, SHUJUNSHA CO., LTD.
""PCR ho saizensen (Forefront of PCR Method)", 1996, KYORITSU SHUPPAN CO.
"honto ni fueru PCR (truly productive PCR)", 1998, SHUJUNSHA CO., article "Bio jikken illustrated (Biological Experiment Illustrated) 3"
KATO, S. ET AL., DNA RES., vol. 12, 2005, pages 53 - 62
TSUGANE, T. ET AL., PLANT BIOTECHNOLOGY., vol. 22, 2005, pages 161 - 165
MURAYAMA, K. ET AL., GENE, vol. 138, 1994, pages 171 - 174
SUZUKI, Y. ET AL., GENE, vol. 200, pages 149 - 156
ALTSCHUL, S. F. ET AL., NUCLEIC ACIDS RES., vol. 25, 1997, pages 3389 - 3402
MIZUTANI, M.; OHTA, D, PLANT PHYSIOL, vol. 116, 1998, pages 357 - 367
SAWAI ET AL., PLANT SCI, vol. 170, 2006, pages 247 - 257
G. GLABRA, RESEARCH CENTER FOR MEDICINAL PLANT RESOURCES
NOMURA T ET AL.: "Cytochrome P450s in plant steroid hormone synthesis and metabolism", PHYTOCHEM.REV., vol. 5, 2006, pages 421 - 432, XP019453412
SHIBUYA, M. ET AL.: "Identification of P-amyrin and sophoradiol 24-hydroxylase by expressed sequence tag mining and functional expression assay", FEBS J., vol. 273, 2006, pages 948 - 959, XP002472147
SEKI, H. ET AL.: "Licorice beta-amyrin 11 oxidase, a cytochrome P450 with a key role in the biosynthesis of the triterpene sweetener glycyrrhizin", PROC.NATL.ACAD.SCI.USA, vol. 105, no. 37, 2008, pages 14204 - 14209, XP008131547
See also references of EP 2336312A4
SHIBUYA ET AL.: "Identification of beta-amyrin and sophoradiol 24-hydroxylase by expressed sequence tag mining and functional expression assay", FEBS J., vol. 273, no. 5, March 2006 (2006-03-01), pages 948 - 59
SAMBROOK, J.: "Molecular Cloning: a Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
"DNA microarray to saishin PCR ho (DNA microarray and the latest PCR method)", 2000, SHUJUNSHA CO., LTD.
""PCR ho saizensen (Forefront of PCR Method)", 1996, KYORITSU SHUPPAN CO.
"honto ni fueru PCR (truly productive PCR)", 1998, SHUJUNSHA CO., article "Bio jikken illustrated (Biological Experiment Illustrated) 3"
KATO, S. ET AL., DNA RES., vol. 12, 2005, pages 53 - 62
TSUGANE, T. ET AL., PLANT BIOTECHNOLOGY., vol. 22, 2005, pages 161 - 165
MURAYAMA, K. ET AL., GENE, vol. 138, 1994, pages 171 - 174
SUZUKI, Y. ET AL., GENE, vol. 200, pages 149 - 156
ALTSCHUL, S. F. ET AL., NUCLEIC ACIDS RES., vol. 25, 1997, pages 3389 - 3402
MIZUTANI, M.; OHTA, D, PLANT PHYSIOL, vol. 116, 1998, pages 357 - 367
SAWAI ET AL., PLANT SCI, vol. 170, 2006, pages 247 - 257
G. GLABRA, RESEARCH CENTER FOR MEDICINAL PLANT RESOURCES
Attorney, Agent or Firm:
HIRAKI Yusuke et al. (JP)
Yusuke Hiraki (JP)
Yusuke Hiraki (JP)
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