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Title:
TRITERPENE OXIDASE DERIVED FROM PLANT BELONGING TO GENUS GLYCHYRRHIZA, GENE ENCODING THE TRITERPENE OXIDASE, AND USE OF THE PROTEIN OR THE GENE
Document Type and Number:
WIPO Patent Application WO/2009/020231
Kind Code:
A1
Abstract:
Disclosed are: a protein having an activity of oxidizing a dammarane triterpene; a gene encoding the protein; and use of the protein or the gene. Specifically disclosed are: a protein having an activity of oxidizing a dammarane triterpene, which is produced from a plant belonging the genus Glychyrrhiza; a gene encoding the protein; and use of the protein or the gene. The protein is depicted in SEQ ID NO: 1, 2 or 13, and the gene encoding the protein is depicted in SEQ ID NO: 3, 4 or 14, respectively. A transformant having the gene introduced therein is also produced, which enables to produce a triterpene oxidase.

Inventors:
MURANAKA, Toshiya (7-22 Suehiro-cho 1-chome, Tsurumi-ku, Yokohama-sh, Kanagawa 45, 2300045, JP)
村中俊哉 (〒45 神奈川県横浜市鶴見区末広町1丁目7番22号独立行政法人理化学研究所 横浜研究所内 Kanagawa, 2300045, JP)
SEKI, Hikaru (7-22 Suehiro-cho 1-chome, Tsurumi-ku, Yokohama-sh, Kanagawa 45, 2300045, JP)
關光 (〒45 神奈川県横浜市鶴見区末広町1丁目7番22号独立行政法人理化学研究所 横浜研究所内 Kanagawa, 2300045, JP)
OHYAMA, Kiyoshi (7-22 Suehiro-cho 1-chome, Tsurumi-ku, Yokohama-sh, Kanagawa 45, 2300045, JP)
大山清 (〒45 神奈川県横浜市鶴見区末広町1丁目7番22号独立行政法人理化学研究所 横浜研究所内 Kanagawa, 2300045, JP)
Application Number:
JP2008/064496
Publication Date:
February 12, 2009
Filing Date:
August 06, 2008
Export Citation:
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Assignee:
RIKEN (2-1 Hirosawa, Wako-shi Saitama, 98, 3510198, JP)
独立行政法人理化学研究所 (〒98 埼玉県和光市広沢2番1号 Saitama, 3510198, JP)
NATIONAL UNIVERSITY CORPORATION CHIBA UNIVERSITY (1-33, Yayoi-cho Inage-ku, Chiba-sh, Chiba 22, 2638522, JP)
株式会社常磐植物化学研究所 (〒01 千葉県佐倉市木野子158番地 Chiba, 2638522, JP)
TOKIWA PHYTOCHEMICAL CO., LTD. (158 Kinoko, Sakura-shi Chiba, 01, 2850801, JP)
TOKIWA PHYTOCHEMICAL CO., LTD. (2850801, JP)
MURANAKA, Toshiya (7-22 Suehiro-cho 1-chome, Tsurumi-ku, Yokohama-sh, Kanagawa 45, 2300045, JP)
村中俊哉 (〒45 神奈川県横浜市鶴見区末広町1丁目7番22号独立行政法人理化学研究所 横浜研究所内 Kanagawa, 2300045, JP)
SEKI, Hikaru (7-22 Suehiro-cho 1-chome, Tsurumi-ku, Yokohama-sh, Kanagawa 45, 2300045, JP)
International Classes:
C12N15/09; A01H5/00; A61K36/48; C12N5/10; C12N9/04; C12P7/02; C12Q1/68; A61K31/56; C07J53/00
Domestic Patent References:
2005-09-01
2005-09-01
Foreign References:
JP2007204769A2007-08-16
Other References:
SHIBUYA M. ET AL.: 'Identification of beta-amyrin and sophoradiol 24-hydroxylase by expressed sequence tag mining and functional expression assay' FEBS J. vol. 273, no. 5, 2006, pages 948 - 959, XP002472147
SEKI H. ET AL.: 'Licorice beta-amyrin 11-oxidase, a cytochrome P450 with a key role in the biosynthesis of the triterpene sweetener glycyrrhizin' PROC. NATL. ACAD SCI. USA vol. 105, no. 37, 2008, pages 14204 - 14209, XP008131547
SHIBUYA ET AL.: 'Identification of beta-amyrin and sophoradiol 24-hydroxylase by expressed sequence tag mining and functional expression assay' FEBS J vol. 273, no. 5, March 2006, pages 948 - 59
'Recent Advances in PCR Methodology', 1996, KYORITSU SHUPPAN CO., LTD.
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See also references of EP 2192183A1
Attorney, Agent or Firm:
HIRAKI, Yusuke et al. (Kamiya-cho MT Bldg. 19F, 3-20 Toranomon 4-chom, Minato-ku Tokyo, 105-0001, JP)
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