Title:
TRYPSIN INHIBITION METHOD, AND METHOD FOR PRODUCING CELL PREPARATION IN WHICH SAME IS USED
Document Type and Number:
WIPO Patent Application WO/2022/210514
Kind Code:
A1
Abstract:
Previously there has not been a method for producing a highly safe cell preparation which, as far as is possible, does not include components of xenogenic origin, in order to reduce cross-species rejection and the risk of transmission of prionic or viral pathogens after using trypsin in the detachment or dispersion of cells. This method for producing a cell preparation includes: a step in which cells are adhered to a substrate and cultivated; a step in which trypsin is used to detach cells from the substrate; and a step in which hPL is brought into contact with both the trypsin and the cells after detachment.
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Inventors:
NAKAISHI TOMOYUKI (JP)
NAKAYAMA SOYA (JP)
IKEBUCHI FUMIE (JP)
MIYOSHI NAKASHIMA SAYAKA (JP)
NAKAYAMA SOYA (JP)
IKEBUCHI FUMIE (JP)
MIYOSHI NAKASHIMA SAYAKA (JP)
Application Number:
PCT/JP2022/014927
Publication Date:
October 06, 2022
Filing Date:
March 28, 2022
Export Citation:
Assignee:
KANEKA CORP (JP)
International Classes:
A61K35/50; A61K35/28; A61P43/00; C12N5/0775
Domestic Patent References:
| WO2020251020A1 | 2020-12-17 |
Foreign References:
| JP2017525340A | 2017-09-07 | |||
| JP2015531594A | 2015-11-05 | |||
| JP2011529706A | 2011-12-15 | |||
| JP2021056659A | 2021-04-08 |
Other References:
"Regenerative Therapy Using Blood-Derived Stem Cells", 15 November 2011, HUMANA PRESS, US, ISBN: 978-1-61779-470-4, article KATHARINA SCHALLMOSER, NATHALIE ETCHART, DIRK STRUNK , EVA ROHDE: "Chapter 5 ; Animal Protein-Free Expansion of Human Mesenchymal Stem/Progenitor Cells", pages: 53 - 69, XP009540273, DOI: 10.1007/978-1-61779-471-1_5
ALMICI, C. : "P540 Expansion of bone marrow derived mesenchymal stem cells: effects of addition of human platelet lysate versus fetal bovine serum in culture's media", BONE MARROW TRANSPLANTATION, vol. 41, no. Suppl. 1, 1 January 2008 (2008-01-01), London , pages S130 - S131, XP009540324, ISSN: 1476-5365
FUSI MARA, DOTTI SILVIA: "Adaptation of the HEp-2 cell line to totally animal-free culture systems and real-time analysis of cell growth", BIOTECHNIQUES, vol. 70, no. 6, 1 June 2021 (2021-06-01), US , pages 319 - 326, XP055974721, ISSN: 0736-6205, DOI: 10.2144/btn-2020-0162
EASTMENT CT ET AL., J CELL PHYSIOL, vol. 97, no. 1, October 1978 (1978-10-01), pages 17 - 27
SCHALLMOSER K ET AL., METHODS MOL BIOL, vol. 946, 2013, pages 349 - 62
ALMICI, C. : "P540 Expansion of bone marrow derived mesenchymal stem cells: effects of addition of human platelet lysate versus fetal bovine serum in culture's media", BONE MARROW TRANSPLANTATION, vol. 41, no. Suppl. 1, 1 January 2008 (2008-01-01), London , pages S130 - S131, XP009540324, ISSN: 1476-5365
FUSI MARA, DOTTI SILVIA: "Adaptation of the HEp-2 cell line to totally animal-free culture systems and real-time analysis of cell growth", BIOTECHNIQUES, vol. 70, no. 6, 1 June 2021 (2021-06-01), US , pages 319 - 326, XP055974721, ISSN: 0736-6205, DOI: 10.2144/btn-2020-0162
EASTMENT CT ET AL., J CELL PHYSIOL, vol. 97, no. 1, October 1978 (1978-10-01), pages 17 - 27
SCHALLMOSER K ET AL., METHODS MOL BIOL, vol. 946, 2013, pages 349 - 62
Attorney, Agent or Firm:
HIRAKI & ASSOCIATES (JP)
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