DONG QING (US)
HUNG GENE (US)
| WO2017004342A1 | 2017-01-05 | |||
| WO2019023417A1 | 2019-01-31 | |||
| WO2018170381A1 | 2018-09-20 | |||
| WO2018081417A2 | 2018-05-03 | |||
| WO2013001310A1 | 2013-01-03 |
| CLAIMS WHAT IS CLAIMED IS: 1. A compound of Formula (II), or a pharmaceutically acceptable salt, solvate, or stereoisomer thereof: wherein: L is a 4-10 atom linker; optionally substituted with one or more RL; each RL is independently deuterium, halogen, -CN, -ORb, -SRb, -S(=O)Ra, -S(=O)2Ra, -NO2, -NRcRd, - NHS(=O)2Ra, -S(=O)2NRcRd, -C(=O)Ra, -OC(=O)Ra, -C(=O)ORb, -OC(=O)ORb, -C(=O)NRcRd, - OC(=O)NRcRd, -NRbC(=O)NRcRd, -NRbC(=O)Ra, -NRbC(=O)ORb, C1-C6alkyl, C1-C6haloalkyl, C1-C6deuteroalkyl, C1-C6hydroxyalkyl, C1-C6aminoalkyl, C2-C6alkenyl, C2-C6alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; or two RL on the same carbon are taken together to form an oxo, a cycloalkyl, or heterocycloalkyl; or two RL on different carbons are taken together to form a cycloalkyl or heterocycloalkyl; Ring A is cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; each RA is independently deuterium, halogen, -CN, -ORb, -SRb, -S(=O)Ra, -S(=O)2Ra, -NO2, -NRcRd, - NHS(=O)2Ra, -S(=O)2NRcRd, -C(=O)Ra, -OC(=O)Ra, -C(=O)ORb, -OC(=O)ORb, -C(=O)NRcRd, - OC(=O)NRcRd, -NRbC(=O)NRcRd, -NRbC(=O)Ra, -NRbC(=O)ORb, C1-C6alkyl, C1-C6haloalkyl, C1-C6deuteroalkyl, C1-C6hydroxyalkyl, C1-C6aminoalkyl, C2-C6alkenyl, C2-C6alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more RA1; or two RA on the same carbon are taken together to form an oxo; each RA1 is independently deuterium, halogen, -CN, -ORb, -SRb, -S(=O)Ra, -S(=O)2Ra, -NO2, -NRcRd, - NHS(=O)2Ra, -S(=O)2NRcRd, -C(=O)Ra, -OC(=O)Ra, -C(=O)ORb, -OC(=O)ORb, -C(=O)NRcRd, - OC(=O)NRcRd, -NRbC(=O)NRcRd, -NRbC(=O)Ra, -NRbC(=O)ORb, C1-C6alkyl, C1-C6haloalkyl, C1-C6deuteroalkyl, C1-C6hydroxyalkyl, C1-C6aminoalkyl, C2-C6alkenyl, C2-C6alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; or two RA1 on the same carbon are taken together to form an oxo n is 0-4; is a single bond or a double bond; X1 and X2 are -N- or -C=; provided that one of X1 or X2 is -N- and the other is -C=; Y8 is CR8 or N; Y6 is CR6 or N; Y3 is CR3 or N; Y9 is CR9 or N; R3, R6, R8, and R9 are independently hydrogen, deuterium, halogen, -CN, -ORb, -SRb, -S(=O)Ra, - S(=O)2Ra, -NO2, -NRcRd, -NHS(=O)2Ra, -S(=O)2NRcRd, -C(=O)Ra, -OC(=O)Ra, -C(=O)ORb, - OC(=O)ORb, -C(=O)NRcRd, -OC(=O)NRcRd, -NRbC(=O)NRcRd, -NRbC(=O)Ra, -NRbC(=O)ORb, C1-C6alkyl, C1-C6haloalkyl, C1-C6deuteroalkyl, C1-C6hydroxyalkyl, C1-C6aminoalkyl, C2-C6alkenyl, or C2-C6alkynyl; R4 is hydrogen, C1-C6alkyl, C1-C6heteroalkyl, C1-C6haloalkyl, C1-C6deuteroalkyl, C1-C6hydroxyalkyl, C1-C6aminoalkyl, C2-C6alkenyl, C2-C6alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl are optionally substituted with one or more R4a; each R4a is independently deuterium, halogen, -CN, -ORb, -NRcRd, -C(=O)Ra, -C(=O)ORb, -C(=O)NRcRd, C1-C6alkyl, C1-C6haloalkyl, C1-C6deuteroalkyl, C1-C6hydroxyalkyl, C1-C6aminoalkyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; or two R4a on the same carbon are taken together to form an oxo; R5 is hydrogen, C1-C6alkyl, C1-C6haloalkyl, or C1-C6deuteroalkyl; R7 is hydrogen, C1-C6alkyl, C1-C6haloalkyl, or C1-C6deuteroalkyl; each Ra is independently C1-C6alkyl, C1-C6haloalkyl, C1-C6deuteroalkyl, C1-C6hydroxyalkyl, C1-C6aminoalkyl, C2-C6alkenyl, C2-C6alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more oxo, deuterium, halogen, -CN, -OH, -OMe, - NH2, -C(=O)Me, -C(=O)OH, -C(=O)OMe, C1-C6alkyl, or C1-C6haloalkyl; each Rb is independently hydrogen, C1-C6alkyl, C1-C6haloalkyl, C1-C6deuteroalkyl, C1-C6hydroxyalkyl, C1-C6aminoalkyl, C2-C6alkenyl, C2-C6alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more oxo, deuterium, halogen, -CN, -OH, -OMe, - NH2, -C(=O)Me, -C(=O)OH, -C(=O)OMe, C1-C6alkyl, or C1-C6haloalkyl; and each Rc and Rd is independently hydrogen, C1-C6alkyl, C1-C6haloalkyl, C1-C6deuteroalkyl, C1-C6hydroxyalkyl, C1-C6aminoalkyl, C2-C6alkenyl, C2-C6alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more oxo, deuterium, halogen, -CN, -OH, -OMe, -NH2, -C(=O)Me, -C(=O)OH, -C(=O)OMe, C1-C6alkyl, or C1-C6haloalkyl; or Rc and Rd are taken together with the nitrogen atom to which they are attached to form a heterocycloalkyl optionally substituted with one or more oxo, deuterium, halogen, -CN, -OH, -OMe, -NH2, -C(=O)Me, -C(=O)OH, -C(=O)OMe, C1-C6alkyl, or C1-C6haloalkyl. 2. The compound of claim 1, or a pharmaceutically acceptable salt, stereoisomer, or solvate thereof, wherein the compound of Formula (II) is of Formula (IIa): 3. The compound of claim 1, or a pharmaceutically acceptable salt, stereoisomer, or solvate thereof, wherein the compound of Formula (II) is of Formula (IIb): 4. The compound of any of claims 1-3, or a pharmaceutically acceptable salt, stereoisomer, or solvate thereof, wherein: Y9 is N. 5. The compound of any of claims 1-4, or a pharmaceutically acceptable salt, stereoisomer, or solvate thereof, wherein: Y6 is CR6. 6. The compound of any of claims 1-5, or a pharmaceutically acceptable salt, stereoisomer, or solvate thereof, wherein: R6 is hydrogen. 7. The compound of any of claims 1-6, or a pharmaceutically acceptable salt, stereoisomer, or solvate thereof, wherein: Y3 is CR3. 8. The compound of any of claims 1-7, or a pharmaceutically acceptable salt, stereoisomer, or solvate thereof, wherein: R3 is hydrogen. 9. The compound of any of claims 1-8, or a pharmaceutically acceptable salt, stereoisomer, or solvate thereof, wherein: Y8 is N. 10. The compound of any of claims 1-8, or a pharmaceutically acceptable salt, stereoisomer, or solvate thereof, wherein: Y8 is CR8. 11. The compound of any of claims 1-8 or 10, or a pharmaceutically acceptable salt, stereoisomer, or solvate thereof, wherein: R8 is hydrogen. 12. The compound of any of claims 1-11, or a pharmaceutically acceptable salt, stereoisomer, or solvate thereof, wherein: R4 is hydrogen, C1-C6alkyl, C1-C6haloalkyl, or C1-C6deuteroalkyl. 13. The compound of any of claims 1-12, or a pharmaceutically acceptable salt, stereoisomer, or solvate thereof, wherein: R4 is C1-C6alkyl or C1-C6deuteroalkyl. 14. The compound of any of claims 1-13, or a pharmaceutically acceptable salt, stereoisomer, or solvate thereof, wherein: R5 is hydrogen. 15. The compound of any of claims 1-14, or a pharmaceutically acceptable salt, stereoisomer, or solvate thereof, wherein: R7 is hydrogen or C1-C6alkyl. 16. The compound of any one of claims 1-15, or a pharmaceutically acceptable salt, stereoisomer, or solvate thereof wherein: Ring A is heterocycloalkyl, aryl, or heteroaryl. 17. The compound of any one of claims 1-16, or a pharmaceutically acceptable salt, stereoisomer, or solvate thereof wherein: Ring A is aryl. 18. The compound of any one of claims 1-16, or a pharmaceutically acceptable salt, stereoisomer, or solvate thereof wherein: Ring A is heteroaryl. 19. The compound of any one of claims 1-18, or a pharmaceutically acceptable salt, stereoisomer, or solvate thereof wherein: each RA is independently deuterium, halogen, -CN, -ORb, -NRcRd, -C(=O)Ra, C1-C6alkyl, C1-C6haloalkyl, or C1-C6deuteroalkyl. 20. The compound of any one of claims 1-19, or a pharmaceutically acceptable salt, stereoisomer, or solvate thereof wherein: L is a 4-8 atom linker; optionally substituted with one or more RL. 21. The compound of any one of claims 1-19, or a pharmaceutically acceptable salt, stereoisomer, or solvate thereof wherein: L is a 4-6 atom linker; optionally substituted with one or more RL. 22. The compound of any one of claims 1-19, or a pharmaceutically acceptable salt, stereoisomer, or solvate thereof wherein: L is a 4-10 atom linker comprising between 4 and 10 carbons and between 0 and 4 heteroatoms selected from oxygen and nitrogen; the linker being optionally substituted with one or more RL. 23. The compound of any one of claims 1-19, or a pharmaceutically acceptable salt, stereoisomer, or solvate thereof wherein: L is a 4-10 atom linker comprising between 3 and 9 carbons and between 1 and 2 heteroatoms selected from oxygen and nitrogen; the linker being optionally substituted with one or more RL. 24. The compound of any one of claims 1-19, or a pharmaceutically acceptable salt, stereoisomer, or solvate thereof wherein: L is a 4-8 atom linker comprising between 4 and 8 carbons and between 0 and 4 heteroatoms selected from oxygen and nitrogen; the linker being optionally substituted with one or more RL. 25. The compound of any one of claims 1-19, or a pharmaceutically acceptable salt, stereoisomer, or solvate thereof wherein: L is a 4-8 atom linker comprising between 3 and 7 carbons and between 1 and 2 heteroatoms selected from oxygen and nitrogen; the linker being optionally substituted with one or more RL. 26. The compound of any one of claims 1-19, or a pharmaceutically acceptable salt, stereoisomer, or solvate thereof wherein: L is a 4-6 atom linker comprising between 4 and 6 carbons and between 0 and 4 heteroatoms selected from oxygen and nitrogen; the linker being optionally substituted with one or more RL. 27. The compound of any one of claims 1-19, or a pharmaceutically acceptable salt, stereoisomer, or solvate thereof wherein: L is a 4-6 atom linker comprising between 3 and 5 carbons and between 1 and 2 heteroatoms selected from oxygen and nitrogen; the linker being optionally substituted with one or more RL. 28. The compound of any one of claims 1-19, or a pharmaceutically acceptable salt, stereoisomer, or solvate thereof wherein: each RL is independently deuterium, halogen, -CN, -ORb, -NRcRd, C1-C6alkyl, C1-C6haloalkyl, or C1-C6deuteroalkyl; or two RL on the same carbon are taken together to form an oxo. 29. The compound of any one of claims 1-28, or a pharmaceutically acceptable salt, stereoisomer, or solvate thereof wherein: each RL is independently deuterium, halogen, C1-C6alkyl, C1-C6haloalkyl, or C1-C6deuteroalkyl; or two RL on the same carbon are taken together to form an oxo or a cycloalkyl; or two RL on different carbons are taken together to form a cycloalkyl. 30. The compound of any one of claims 1-29, or a pharmaceutically acceptable salt, stereoisomer, or solvate thereof wherein: each RL is independently deuterium, halogen, C1-C6alkyl, C1-C6haloalkyl, or C1-C6deuteroalkyl; or two RL on the same carbon are taken together to form an oxo. 31. The compound of any one of claims 1-29, or a pharmaceutically acceptable salt, stereoisomer, or solvate thereof wherein: 32. The compound of any one of claims 1-30, or a pharmaceutically acceptable salt, stereoisomer, or solvate thereof wherein: 33. The compound of any one of claims 1-31, or a pharmaceutically acceptable salt, stereoisomer, or solvate thereof wherein: 34. The compound of claim 1, or a pharmaceutically acceptable salt, stereoisomer, or solvate thereof wherein the compound is: 35. A pharmaceutical composition comprising a therapeutically effective amount of the compound of any one of claims 1-34, or a pharmaceutically acceptable salt, stereoisomer, or solvate thereof, and a pharmaceutically acceptable excipient. 36. A method of inhibiting a TYK2 enzyme in a patient or biological sample comprising contacting said patient or biological sample with a compound of any one of claims 1-34, or a pharmaceutically acceptable salt, stereoisomer, or solvate thereof. 37. A method of treating a TYK2-mediated disorder comprising administering to a patient in need thereof a compound of any one of claims 1-34, or a pharmaceutically acceptable salt, stereoisomer, or solvate thereof. 38. The method of claim 37, wherein the TYK2-mediated disorder is an autoimmune disorder, an inflammatory disorder, a proliferative disorder, an endocrine disorder, a neurological disorder, or a disorder associated with transplantation. 39. The method of claim 37, wherein the disorder is associated with type I interferon, IL-10, IL-12, or IL-23 signaling. |
[0001] This patent application claims the benefit of US Provisional Application No.62/816,698, filed March 11, 2019; US Provisional Application No.62/835,376, filed April 17, 2019; US Provisional Application No.62/877,741, filed July 23, 2019; and US Provisional Application No.62/931,119, filed November 5, 2019 each of which is incorporated herein by reference in their entirety. FIELD OF THE INVENTION
[0002] Described herein are compounds, methods of making such compounds, pharmaceutical compositions and medicaments comprising such compounds, and methods of using such compounds for inhibiting nonreceptor tyrosine-protein kinase 2 (“TYK2”), also known as Tyrosine kinase 2. BACKGROUND OF THE INVENTION
[0003] TYK2 is a non-receptor tyrosine kinase member of the Janus kinase (JAKs) family of protein kinases. The mammalian JAK family consists of four members, TYK2, JAK1, JAK2, and JAK3. JAK proteins, including TYK2, are integral to cytokine signaling. TYK2 associates with the cytoplasmic domain of type I and type II cytokine receptors, as well as interferon types I and III receptors, and is activated by those receptors upon cytokine binding. Cytokines implicated in TYK2 activation include interferons (e.g. IFN-a, IFN-b, IFN-k, IFN-d, IFN-e, IFN-t, IFN-w, and IFN-z (also known as limitin), and interleukins (e.g. IL-4, IL-6, IL-10, IL-11, IL-12, IL-13, L-22, IL-23, IL-27, IL-31, oncostatin M, ciliary neurotrophic factor, cardiotrophin 1, cardiotrophin-like cytokine, and LIF). The activated TYK2 then goes on to phosphorylate further signaling proteins such as members of the STAT family, including STAT1, STAT2, STAT4, and STAT6.
[0004] TYK2 activation by IL-23, has been linked to inflammatory bowel disease (IBD), Crohn's disease, and ulcerative colitis. A genome-wide association study of 2,622 individuals with psoriasis identified associations between disease susceptibility and TYK2. Knockout or tyrphostin inhibition of TYK2 significantly reduces both IL-23 and IL-22-induced dermatitis.
[0005] TYK2 also plays a role in respiratory diseases such as asthma, chronic obstructive pulmonary disease (COPD), lung cancer, and cystic fibrosis. Goblet cell hyperplasia (GCH) and mucous hypersecretion is mediated by IL-13-induced activation of TYK2, which in turn activates STAT6.
[0006] Decreased TYK2 activity leads to protection of joints from collagen antibody-induced arthritis, a model of human rheumatoid arthritis. Mechanistically, decreased Tyk2 activity reduced the production of Th1/Th17-related cytokines and matrix metalloproteases, and other key markers of inflammation.
[0007] TYK2 knockout mice showed complete resistance in experimental autoimmune
encephalomyelitis (EAE, an animal model of multiple sclerosis (MS)), with no infiltration of CD4 T cells in the spinal cord, as compared to controls, suggesting that TYK2 is essential to pathogenic CD4- mediated disease development in MS. This corroborates earlier studies linking increased TYK2 expression with MS susceptibility. Loss of function mutation in TYK2, leads to decreased demyelination and increased remyelination of neurons, further suggesting a role for TYK2 inhibitors in the treatment of MS and other CNS demyelination disorders.
[0008] TYK2 is the sole signaling messenger common to both IL-12 and IL-23. TYK2 knockout reduced methylated BSA injection-induced footpad thickness, imiquimod-induced psoriasis-like skin inflammation, and dextran sulfate sodium or 2,4,6-trinitrobenzene sulfonic acid-induced colitis in mice.
[0009] Joint linkage and association studies of various type I IFN signaling genes with systemic lupus erythematosus (SLE, an autoimmune disorder), showed a strong, and significant correlation between loss of function mutations to TYK2 and decreased prevalence of SLE in families with affected members. Genome-wide association studies of individuals with SLE versus an unaffected cohort showed highly significant correlation between the TYK2 locus and SLE.
[0010] TYK2 has been shown to play an important role in maintaining tumor surveillance and TYK2 knockout mice showed compromised cytotoxic T cell response, and accelerated tumor development. However, these effects were linked to the efficient suppression of natural killer (NK) and cytotoxic T lymphocytes, suggesting that TYK2 inhibitors would be highly suitable for the treatment of autoimmune disorders or transplant rejection. Although other JAK family members such as JAK3 have similar roles in the immune system, TYK2 has been suggested as a superior target because of its involvement in fewer and more closely related signaling pathways, leading to fewer off-target effects.
[0011] Studies in T-cell acute lymphoblastic leukemia (T-ALL) indicate that T-ALL is highly dependent on IL-10 via TYK2 via STAT1-mediated signal transduction to maintain cancer cell survival through upregulation of anti-apoptotic protein BCL2. Knockdown of TYK2, but not other JAK family members, reduced cell growth. Specific activating mutations to TYK2 that promote cancer cell survival include those to the FERM domain (G36D, S47N, and R425H), the JH2 domain (V731I), and the kinase domain (E957D and R1027H). However, it was also identified that the kinase function of TYK2 is required for increased cancer cell survival, as TYK2 enzymes featuring kinase-dead mutations (M978Y or M978F) in addition to an activating mutation (E957D) resulted in failure to transform.
[0012] Thus, selective inhibition of TYK2 has been suggested as a suitable target for patients with IL- 10 and/or BCL2-addicted tumors, such as 70% of adult T-cell leukemia cases. TYK2 mediated STAT3 signaling has also been shown to mediate neuronal cell death caused by amyloid-b (Ab) peptide.
Decreased TYK2 phosphorylation of STAT3 following Ab administration lead to decreased neuronal cell death, and increased phosphorylation of STAT3 has been observed in postmortem brains of Alzheimer's patients.
[0013] Inhibition of JAK-STAT signaling pathways is also implicated in hair growth, and the reversal of the hair loss associated with alopecia areata.
[0014] Accordingly, compounds that inhibit the activity of TYK2 are beneficial, especially those with selectivity over JAK2. Such compounds should deliver a pharmacological response that favorably treats one or more of the conditions described herein without the side-effects associated with the inhibition of JAK2.
[0015] Accordingly there is a need to provide novel inhibitors having more effective or advantageous pharmaceutically relevant properties, like selectivity over other JAK kinases (especially JAK2).
BRIEF SUMMARY OF THE INVENTION
[0016] Disclosed herein is a compound of Formula (II), or a pharmaceutically acceptable salt, solvate, or stereoisomer thereof:
wherein:
L is a 4-10 atom linker; optionally substituted with one or more R L ;
each R L is independently deuterium, halogen, -CN, -OR b , -SR b , -S(=O)R a , -S(=O) 2 R a , -NO 2 , -NR c R d , - NHS(=O) 2 R a , -S(=O) 2 NR c R d , -C(=O)R a , -OC(=O)R a , -C(=O)OR b , -OC(=O)OR b , -C(=O)NR c R d , - OC(=O)NR c R d , -NR b C(=O)NR c R d , -NR b C(=O)R a , -NR b C(=O)OR b , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; or two R L on the same carbon are taken together to form an oxo, a cycloalkyl, or heterocycloalkyl; or two R L on different carbons are taken together to form a cycloalkyl or heterocycloalkyl;
Ring A is cycloalkyl, heterocycloalkyl, aryl, or heteroaryl;
each R A is independently deuterium, halogen, -CN, -OR b , -SR b , -S(=O)R a , -S(=O) 2 R a , -NO 2 , -NR c R d , - NHS(=O) 2 R a , -S(=O) 2 NR c R d , -C(=O)R a , -OC(=O)R a , -C(=O)OR b , -OC(=O)OR b , -C(=O)NR c R d , - OC(=O)NR c R d , -NR b C(=O)NR c R d , -NR b C(=O)R a , -NR b C(=O)OR b , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, alkenyl, alkynyl, cycloalkyl,
heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more R A1 ; or two R A on the same carbon are taken together to form an oxo;
each R A1 is independently deuterium, halogen, -CN, -OR b , -SR b , -S(=O)R a , -S(=O) 2 R a , -NO 2 , -NR c R d , - NHS(=O) 2 R a , -S(=O) 2 NR c R d , -C(=O)R a , -OC(=O)R a , -C(=O)OR b , -OC(=O)OR b , -C(=O)NR c R d , - OC(=O)NR c R d , -NR b C(=O)NR c R d , -NR b C(=O)R a , -NR b C(=O)OR b , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; or two R A1 on the same carbon are taken together to form an oxo
n is 0-4;
is a single bond or a double bond;
X 1 and X 2 are -N- or -C=; provided that one of X 1 or X 2 is -N- and the other is -C=;
Y 8 is CR 8 or N;
Y 6 is CR 6 or N;
Y 3 is CR 3 or N;
Y 9 is CR 9 or N;
R 3 , R 6 , R 8 , and R 9 are independently hydrogen, deuterium, halogen, -CN, -OR b , -SR b , -S(=O)R a , - S(=O) 2 R a , -NO 2 , -NR c R d , -NHS(=O) 2 R a , -S(=O) 2 NR c R d , -C(=O)R a , -OC(=O)R a , -C(=O)OR b , - OC(=O)OR b , -C(=O)NR c R d , -OC(=O)NR c R d , -NR b C(=O)NR c R d , -NR b C(=O)R a , -NR b C(=O)OR b , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, or C 2 -C 6 alkynyl;
R 4 is hydrogen, C 1 -C 6 alkyl, C 1 -C 6 heteroalkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl are optionally substituted with one or more R 4a ;
each R 4a is independently deuterium, halogen, -CN, -OR b , -NR c R d , -C(=O)R a , -C(=O)OR b , -C(=O)NR c R d , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; or two R 4a on the same carbon are taken together to form an oxo;
R 5 is hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, or C 1 -C 6 deuteroalkyl;
R 7 is hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, or C 1 -C 6 deuteroalkyl;
each R a is independently C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl,
C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is
independently optionally substituted with one or more oxo, deuterium, halogen, -CN, -OH, -OMe, - NH 2 , -C(=O)Me, -C(=O)OH, -C(=O)OMe, C 1 -C 6 alkyl, or C 1 -C 6 haloalkyl;
each R b is independently hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is
independently optionally substituted with one or more oxo, deuterium, halogen, -CN, -OH, -OMe, - NH 2 , -C(=O)Me, -C(=O)OH, -C(=O)OMe, C 1 -C 6 alkyl, or C 1 -C 6 haloalkyl; and
each R c and R d is independently hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl,
C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more oxo, deuterium, halogen, -CN, -OH, -OMe, -NH 2 , -C(=O)Me, -C(=O)OH, -C(=O)OMe, C 1 -C 6 alkyl, or C 1 -C 6 haloalkyl;
o r Rc and Rd are taken together with the nitrogen atom to which they are attached to form a
heterocycloalkyl optionally substituted with one or more oxo, deuterium, halogen, -CN, -OH, -OMe, -NH 2 , -C(=O)Me, -C(=O)OH, -C(=O)OMe, C 1 -C 6 alkyl, or C 1 -C 6 haloalkyl.
[0017] Also disclosed herein is a compound of Formula (IIa), or a pharmaceutically acceptable salt, stereoisomer, or solvate thereof:
[0018] Also disclosed herein is a compound of Formula (IIb), or a pharmaceutically acceptable salt, stereoisomer, or solvate thereof:
[0019] Also disclosed herein is a pharmaceutical composition comprising a therapeutically effective amount of the compound disclosed herein, or a pharmaceutically acceptable salt, stereoisomer, or solvate thereof, and a pharmaceutically acceptable excipient.
[0020] Also disclosed herein is a method of inhibiting a TYK2 enzyme in a patient or biological sample comprising contacting said patient or biological sample with a compound disclosed herein, or a pharmaceutically acceptable salt, stereoisomer, or solvate thereof.
[0021] Also disclosed herein is a method of treating a TYK2-mediated disorder comprising administering to a patient in need thereof a compound disclosed herein, or a pharmaceutically acceptable salt, stereoisomer, or solvate thereof. In some embodiments, the TYK2-mediated disorder is an autoimmune disorder, an inflammatory disorder, a proliferative disorder, an endocrine disorder, a neurological disorder, or a disorder associated with transplantation. In some embodiments, the disorder is associated with type I interferon, IL-10, IL-12, or IL-23 signaling. INCORPORATION BY REFERENCE
[0022] All publications, patents, and patent applications mentioned in this specification are herein incorporated by reference for the specific purposes identified herein. DETAILED DESCRIPTION OF THE INVENTION
Definitions
[0023] As used herein and in the appended claims, the singular forms“a,”“an,” and“the” include plural referents unless the context clearly dictates otherwise. Thus, for example, reference to“an agent” includes a plurality of such agents, and reference to“the cell” includes reference to one or more cells (or to a plurality of cells) and equivalents thereof known to those skilled in the art, and so forth. When ranges are used herein for physical properties, such as molecular weight, or chemical properties, such as chemical formulae, all combinations and subcombinations of ranges and specific embodiments therein are intended to be included. The term“about” when referring to a number or a numerical range means that the number or numerical range referred to is an approximation within experimental variability (or within statistical experimental error), and thus the number or numerical range, in some instances, will vary between 1% and 15% of the stated number or numerical range. The term“comprising” (and related terms such as“comprise” or“comprises” or“having” or“including”) is not intended to exclude that in other certain embodiments, for example, an embodiment of any composition of matter, composition, method, or process, or the like, described herein,“consist of” or“consist essentially of” the described features.
[0024] As used in the specification and appended claims, unless specified to the contrary, the following terms have the meaning indicated below.
[0025] “Aliphatic chain” refers to a linear chemical moiety that is composed of only carbons and hydrogens. In some embodiments, the aliphatic chain is saturated. In some embodiments, the aliphatic chain is unsaturated. In some embodiments, the unsaturated aliphatic chain contains one unsaturation. In some embodiments, the unsaturated aliphatic chain contains more than one unsaturation. In some embodiments, the unsaturated aliphatic chain contains two unsaturations. In some embodiments, the unsaturated aliphatic chain contains one double bond. In some embodiments, the unsaturated aliphatic chain contains two double bonds.
[0026] “Oxo” refers to =O.
[0027] “Alkyl” refers to an optionally substituted straight-chain, or optionally substituted branched- chain saturated hydrocarbon monoradical having from one to about ten carbon atoms, or from one to six carbon atoms. Examples include, but are not limited to, methyl, ethyl, n-propyl, isopropyl, 2-methyl-1- propyl, 2-methyl-2-propyl, 2-methyl-1-butyl, 3-methyl-1-butyl, 2-methyl-3-butyl, 2,2-dimethyl-1-propyl, 2-methyl-1-pentyl, 3-methyl-1-pentyl, 4-methyl-1-pentyl, 2-methyl-2-pentyl, 3-methyl-2-pentyl, 4- methyl-2-pentyl, 2,2-dimethyl-1-butyl, 3,3-dimethyl-1-butyl, 2-ethyl-1-butyl, n-butyl, isobutyl, sec-butyl, t-butyl, n-pentyl, isopentyl, neopentyl, tert-amyl and hexyl, and longer alkyl groups, such as heptyl, octyl, and the like. Whenever it appears herein, a numerical range such as“C 1 -C 6 alkyl” means that the alkyl group consists of 1 carbon atom, 2 carbon atoms, 3 carbon atoms, 4 carbon atoms, 5 carbon atoms or 6 carbon atoms, although the present definition also covers the occurrence of the term“alkyl” where no numerical range is designated. In some embodiments, the alkyl is a C 1 -C 10 alkyl, a C 1 -C 9 alkyl, a C 1 -C 8 alkyl, a C 1 -C 7 alkyl, a C 1 -C 6 alkyl, a C 1 -C 5 alkyl, a C 1 -C 4 alkyl, a C 1 -C 3 alkyl, a C 1 -C 2 alkyl, or a C 1 alkyl. Unless stated otherwise specifically in the specification, an alkyl group is optionally substituted, for example, with oxo, halogen, amino, nitrile, nitro, hydroxyl, haloalkyl, alkoxy, aryl, cycloalkyl, heterocycloalkyl, heteroaryl, and the like. In some embodiments, the alkyl is optionally substituted with oxo, halogen, -CN, -CF 3 , -OH, -OMe, -NH 2 , or -NO 2 . In some embodiments, the alkyl is optionally substituted with oxo, halogen, -CN, -CF 3 , -OH, or -OMe. In some embodiments, the alkyl is optionally substituted with halogen.
[0028] “Alkenyl” refers to an optionally substituted straight-chain, or optionally substituted branched- chain hydrocarbon monoradical having one or more carbon-carbon double-bonds and having from two to about ten carbon atoms, more preferably two to about six carbon atoms. The group may be in either the cis or trans conformation about the double bond(s), and should be understood to include both isomers. Examples include, but are not limited to, ethenyl (-CH=CH 2 ), 1-propenyl (-CH 2 CH=CH 2 ), isopropenyl [-C(CH 3 )=CH 2 ], butenyl, 1,3-butadienyl and the like. Whenever it appears herein, a numerical range such as“C 2 -C 6 alkenyl” means that the alkenyl group may consist of 2 carbon atoms, 3 carbon atoms, 4 carbon atoms, 5 carbon atoms or 6 carbon atoms, although the present definition also covers the occurrence of the term“alkenyl” where no numerical range is designated. In some embodiments, the alkenyl is a C 2 -C 10 alkenyl, a C 2 -C 9 alkenyl, a C 2 -C 8 alkenyl, a C 2 -C 7 alkenyl, a C 2 -C 6 alkenyl, a C 2 -C 5 alkenyl, a C 2 -C 4 alkenyl, a C 2 -C 3 alkenyl, or a C2 alkenyl. Unless stated otherwise specifically in the specification, an alkenyl group is optionally substituted, for example, with oxo, halogen, amino, nitrile, nitro, hydroxyl, haloalkyl, alkoxy, aryl, cycloalkyl, heterocycloalkyl, heteroaryl, and the like. In some embodiments, an alkenyl is optionally substituted with oxo, halogen, -CN, -CF 3 , -OH, -OMe, -NH 2 , or -NO 2 . In some embodiments, an alkenyl is optionally substituted with oxo, halogen, -CN, -CF 3 , -OH, or -OMe. In some embodiments, the alkenyl is optionally substituted with halogen.
[0029] “Alkynyl” refers to an optionally substituted straight-chain or optionally substituted branched- chain hydrocarbon monoradical having one or more carbon-carbon triple-bonds and having from two to about ten carbon atoms, more preferably from two to about six carbon atoms. Examples include, but are not limited to, ethynyl, 2-propynyl, 2-butynyl, 1,3-butadiynyl and the like. Whenever it appears herein, a numerical range such as“C 2 -C 6 alkynyl” means that the alkynyl group may consist of 2 carbon atoms, 3 carbon atoms, 4 carbon atoms, 5 carbon atoms or 6 carbon atoms, although the present definition also covers the occurrence of the term“alkynyl” where no numerical range is designated. In some embodiments, the alkynyl is a C 2 -C 10 alkynyl, a C 2 -C 9 alkynyl, a C 2 -C 8 alkynyl, a C 2 -C 7 alkynyl, a C 2 -C 6 alkynyl, a C 2 -C 5 alkynyl, a C 2 -C 4 alkynyl, a C 2 -C 3 alkynyl, or a C 2 alkynyl. Unless stated otherwise specifically in the specification, an alkynyl group is optionally substituted, for example, with oxo, halogen, amino, nitrile, nitro, hydroxyl, haloalkyl, alkoxy, aryl, cycloalkyl, heterocycloalkyl, heteroaryl, and the like. In some embodiments, an alkynyl is optionally substituted with oxo, halogen, -CN, - CF 3 , -OH, -OMe, -NH 2 , or -NO 2 . In some embodiments, an alkynyl is optionally substituted with oxo, halogen, -CN, -CF 3 , -OH, or -OMe. In some embodiments, the alkynyl is optionally substituted with halogen.
[0030] “Alkylene” refers to a straight or branched divalent hydrocarbon chain. Unless stated otherwise specifically in the specification, an alkylene group may be optionally substituted, for example, with oxo, halogen, amino, nitrile, nitro, hydroxyl, haloalkyl, alkoxy, aryl, cycloalkyl, heterocycloalkyl, heteroaryl, and the like. In some embodiments, an alkylene is optionally substituted with oxo, halogen, -CN, - CF 3 , -OH, -OMe, -NH 2 , or -NO 2 . In some embodiments, an alkylene is optionally substituted with oxo, halogen, -CN, -CF 3 , -OH, or -OMe. In some embodiments, the alkylene is optionally substituted with halogen.
[0031] “Alkoxy” refers to a radical of the formula -OR a where R a is an alkyl radical as defined. Unless stated otherwise specifically in the specification, an alkoxy group may be optionally substituted, for example, with oxo, halogen, amino, nitrile, nitro, hydroxyl, haloalkyl, alkoxy, aryl, cycloalkyl, heterocycloalkyl, heteroaryl, and the like. In some embodiments, an alkoxy is optionally substituted with oxo, halogen, -CN, -CF 3 , -OH, -OMe, -NH 2 , or -NO 2 . In some embodiments, an alkoxy is optionally substituted with oxo, halogen, -CN, -CF 3 , -OH, or -OMe. In some embodiments, the alkoxy is optionally substituted with halogen.
[0032] “Aminoalkyl” refers to an alkyl radical, as defined above, that is substituted by one or more amines. In some embodiments, the alkyl is substituted with one amine. In some embodiments, the alkyl is substituted with one, two, or three amines. Aminoalkyl include, for example, aminomethyl, aminoethyl, aminopropyl, aminobutyl, or aminopentyl. In some embodiments, the aminoalkyl is aminomethyl.
[0033] “Aryl” refers to a radical derived from a hydrocarbon ring system comprising hydrogen, 6 to 30 carbon atoms and at least one aromatic ring. The aryl radical may be a monocyclic, bicyclic, tricyclic or tetracyclic ring system, which may include fused (when fused with a cycloalkyl or heterocycloalkyl ring, the aryl is bonded through an aromatic ring atom) or bridged ring systems. In some embodiments, the aryl is a 6- to 10-membered aryl. In some embodiments, the aryl is a 6-membered aryl. Aryl radicals include, but are not limited to, aryl radicals derived from the hydrocarbon ring systems of anthrylene, naphthylene, phenanthrylene, anthracene, azulene, benzene, chrysene, fluoranthene, fluorene, as- indacene, s-indacene, indane, indene, naphthalene, phenalene, phenanthrene, pleiadene, pyrene, and triphenylene. In some embodiments, the aryl is phenyl. Unless stated otherwise specifically in the specification, an aryl may be optionally substituted, for example, with halogen, amino, nitrile, nitro, hydroxyl, alkyl, alkenyl, alkynyl, haloalkyl, alkoxy, aryl, cycloalkyl, heterocycloalkyl, heteroaryl, and the like. In some embodiments, an aryl is optionally substituted with halogen, methyl, ethyl, -CN, - CF 3 , -OH, -OMe, -NH 2 , or -NO 2 . In some embodiments, an aryl is optionally substituted with halogen, methyl, ethyl, -CN, -CF 3 , -OH, or -OMe. In some embodiments, the aryl is optionally substituted with halogen.
[0034] “Cycloalkyl” refers to a partially or fully saturated, monocyclic or polycyclic carbocyclic ring, which may include fused (when fused with an aryl or a heteroaryl ring, the cycloalkyl is bonded through a non-aromatic ring atom) or bridged ring systems. Representative cycloalkyls include, but are not limited to, cycloalkyls having from three to fifteen carbon atoms (C 3 -C 15 cycloalkyl), from three to ten carbon atoms (C 3 -C 10 cycloalkyl), from three to eight carbon atoms (C 3 -C 8 cycloalkyl), from three to six carbon atoms (C 3 -C 6 cycloalkyl), from three to five carbon atoms (C 3 -C 5 cycloalkyl), or three to four carbon atoms (C 3 -C 4 cycloalkyl). In some embodiments, the cycloalkyl is a 3- to 6-membered cycloalkyl. In some embodiments, the cycloalkyl is a 5- to 6-membered cycloalkyl. Monocyclic cycloalkyls include, for example, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, and cyclooctyl. Polycyclic cycloalkyls or carbocycles include, for example, adamantyl, norbornyl, decalinyl, bicyclo[3.3.0]octane, bicyclo[4.3.0]nonane, cis-decalin, trans-decalin, bicyclo[2.1.1]hexane, bicyclo[2.2.1]heptane, bicyclo[2.2.2]octane, bicyclo[3.2.2]nonane, and bicyclo[3.3.2]decane, and
7,7-dimethyl-bicyclo[2.2.1]heptanyl. Partially saturated cycloalkyls include, for example cyclopentenyl, cyclohexenyl, cycloheptenyl, and cyclooctenyl. Unless stated otherwise specifically in the specification, a cycloalkyl is optionally substituted, for example, with oxo, halogen, amino, nitrile, nitro, hydroxyl, alkyl, alkenyl, alkynyl, haloalkyl, alkoxy, aryl, cycloalkyl, heterocycloalkyl, heteroaryl, and the like. In some embodiments, a cycloalkyl is optionally substituted with oxo, halogen, methyl, ethyl, -CN, - CF 3 , -OH, -OMe, -NH 2 , or -NO 2 . In some embodiments, a cycloalkyl is optionally substituted with oxo, halogen, methyl, ethyl, -CN, -CF 3 , -OH, or -OMe. In some embodiments, the cycloalkyl is optionally substituted with halogen.
[0035] “Deuteroalkyl” refers to an alkyl radical, as defined above, that is substituted by one or more deuterium atoms. In some embodiments, the alkyl is substituted with one deuterium atom. In some embodiments, the alkyl is substituted with one, two, or three deuterium atoms. In some embodiments, the alkyl is substituted with one, two, three, four, five, or six deuterium atoms. Deuteroalkyl includes, for example, CD 3 , CH 2 D, CHD 2 , CH 2 CD 3 , CD 2 CD 3 , CHDCD 3 , CH 2 CH 2 D, or CH 2 CHD 2 . In some embodiments, the deuteroalkyl is CD 3 .
[0036] “Haloalkyl” refers to an alkyl radical, as defined above, that is substituted by one or more halogen atoms. In some embodiments, the alkyl is substituted with one, two, or three halogen atoms. In some embodiments, the alkyl is substituted with one, two, three, four, five, or six halogen halogens. Haloalkyl includes, for example, trifluoromethyl, difluoromethyl, fluoromethyl, trichloromethyl, 2,2,2-trifluoroethyl, 1,2-difluoroethyl, 3-bromo-2-fluoropropyl, 1,2-dibromoethyl, and the like. In some embodiments, the haloalkyl is trifluoromethyl.
[0037] “Halo” or“halogen” refers to bromo, chloro, fluoro or iodo. In some embodiments, halogen is fluoro or chloro. In some embodiments, halogen is fluoro. [0038] “Heteroalkyl” refers to an alkyl group in which one or more skeletal atoms of the alkyl are selected from an atom other than carbon, e.g., oxygen, nitrogen (e.g., -NH-, -N(alkyl)-), sulfur, or combinations thereof. A heteroalkyl is attached to the rest of the molecule at a carbon atom of the heteroalkyl. In one aspect, a heteroalkyl is a C 1 -C 6 heteroalkyl wherein the heteroalkyl is comprised of 1 to 6 carbon atoms and one or more atoms other than carbon, e.g., oxygen, nitrogen (e.g. -NH-, -N(alkyl)- ), sulfur, or combinations thereof wherein the heteroalkyl is attached to the rest of the molecule at a carbon atom of the heteroalkyl. Examples of such heteroalkyl are, for example, -CH 2 OCH 3 , - CH 2 CH 2 OCH 3 , -CH 2 CH 2 OCH 2 CH 2 OCH 3 , or -CH(CH 3 )OCH 3 . Unless stated otherwise specifically in the specification, a heteroalkyl is optionally substituted for example, with oxo, halogen, amino, nitrile, nitro, hydroxyl, alkyl, alkenyl, alkynyl, haloalkyl, alkoxy, aryl, cycloalkyl, heterocycloalkyl, heteroaryl, and the like. In some embodiments, a heteroalkyl is optionally substituted with oxo, halogen, methyl, ethyl, - CN, -CF 3 , -OH, -OMe, -NH 2 , or -NO 2 . In some embodiments, a heteroalkyl is optionally substituted with oxo, halogen, methyl, ethyl, -CN, -CF 3 , -OH, or -OMe. In some embodiments, the heteroalkyl is optionally substituted with halogen.
[0039] “Hydroxyalkyl” refers to an alkyl radical, as defined above, that is substituted by one or more hydroxyls. In some embodiments, the alkyl is substituted with one hydroxyl. In some embodiments, the alkyl is substituted with one, two, or three hydroxyls. Hydroxyalkyl include, for example,
hydroxymethyl, hydroxyethyl, hydroxypropyl, hydroxybutyl, or hydroxypentyl. In some embodiments, the hydroxyalkyl is hydroxymethyl.
[0040] “Heterocycloalkyl” refers to a 3- to 24-membered partially or fully saturated ring radical comprising 2 to 23 carbon atoms and from one to 8 heteroatoms selected from the group consisting of nitrogen, oxygen, phosphorous and sulfur. In some embodiments, the heterocycloalkyl comprises 1 or 2 heteroatoms selected from nitrogen and oxygen. Unless stated otherwise specifically in the specification, the heterocycloalkyl radical may be a monocyclic, bicyclic, tricyclic or tetracyclic ring system, which may include fused (when fused with an aryl or a heteroaryl ring, the heterocycloalkyl is bonded through a non-aromatic ring atom) or bridged ring systems; and the nitrogen, carbon or sulfur atoms in the heterocycloalkyl radical may be optionally oxidized; the nitrogen atom may be optionally quaternized. Representative heterocycloalkyls include, but are not limited to, heterocycloalkyls having from two to fifteen carbon atoms (C 2 -C 15 heterocycloalkyl), from two to ten carbon atoms (C 2 -C 10 heterocycloalkyl), from two to eight carbon atoms (C 2 -C 8 heterocycloalkyl), from two to six carbon atoms (C 2 -C 6 heterocycloalkyl), from two to five carbon atoms (C 2 -C 5 heterocycloalkyl), or two to four carbon atoms (C 2 -C 4 heterocycloalkyl). In some embodiments, the heterocycloalkyl is a 3- to 6-membered
heterocycloalkyl. In some embodiments, the cycloalkyl is a 5- to 6-membered heterocycloalkyl.
Examples of such heterocycloalkyl radicals include, but are not limited to, aziridinyl, azetidinyl, dioxolanyl, thienyl[1,3]dithianyl, decahydroisoquinolyl, imidazolinyl, imidazolidinyl, isothiazolidinyl, isoxazolidinyl, morpholinyl, octahydroindolyl, octahydroisoindolyl, 2-oxopiperazinyl, 2-oxopiperidinyl, 2-oxopyrrolidinyl, oxazolidinyl, piperidinyl, piperazinyl, 4-piperidonyl, pyrrolidinyl, pyrazolidinyl, quinuclidinyl, thiazolidinyl, tetrahydrofuryl, trithianyl, tetrahydropyranyl, thiomorpholinyl, thiamorpholinyl, 1-oxo-thiomorpholinyl, 1,1-dioxo-thiomorpholinyl, 1,3-dihydroisobenzofuran-1-yl, 3- oxo-1,3-dihydroisobenzofuran-1-yl, methyl-2-oxo-1,3-dioxol-4-yl, and 2-oxo-1,3-dioxol-4-yl. The term heterocycloalkyl also includes all ring forms of the carbohydrates, including but not limited to, the monosaccharides, the disaccharides and the oligosaccharides. It is understood that when referring to the number of carbon atoms in a heterocycloalkyl, the number of carbon atoms in the heterocycloalkyl is not the same as the total number of atoms (including the heteroatoms) that make up the heterocycloalkyl (i.e. skeletal atoms of the heterocycloalkyl ring). Unless stated otherwise specifically in the specification, a heterocycloalkyl is optionally substituted, for example, with oxo, halogen, amino, nitrile, nitro, hydroxyl, alkyl, alkenyl, alkynyl, haloalkyl, alkoxy, aryl, cycloalkyl, heterocycloalkyl, heteroaryl, and the like. In some embodiments, a heterocycloalkyl is optionally substituted with oxo, halogen, methyl, ethyl, -CN, - CF 3 , -OH, -OMe, -NH 2 , or -NO 2 . In some embodiments, a heterocycloalkyl is optionally substituted with oxo, halogen, methyl, ethyl, -CN, -CF 3 , -OH, or -OMe. In some embodiments, the heterocycloalkyl is optionally substituted with halogen.
[0041] “Heteroalkyl” refers to an alkyl group in which one or more skeletal atoms of the alkyl are selected from an atom other than carbon, e.g., oxygen, nitrogen (e.g. -NH-, -N(alkyl)-), sulfur, or combinations thereof. A heteroalkyl is attached to the rest of the molecule at a carbon atom of the heteroalkyl. In one aspect, a heteroalkyl is a C 1 -C 6 heteroalkyl. Unless stated otherwise specifically in the specification, a heteroalkyl is optionally substituted, for example, with oxo, halogen, amino, nitrile, nitro, hydroxyl, alkyl, alkenyl, alkynyl, haloalkyl, alkoxy, aryl, cycloalkyl, heterocycloalkyl, heteroaryl, and the like. In some embodiments, a heteroalkyl is optionally substituted with oxo, halogen, methyl, ethyl, - CN, -CF 3 , -OH, -OMe, -NH 2 , or -NO 2 . In some embodiments, a heteroalkyl is optionally substituted with oxo, halogen, methyl, ethyl, -CN, -CF 3 , -OH, or -OMe. In some embodiments, the heteroalkyl is optionally substituted with halogen.
[0042] “Heteroaryl” refers to a 5- to 14-membered ring system radical comprising hydrogen atoms, one to thirteen carbon atoms, one to six heteroatoms selected from the group consisting of nitrogen, oxygen, phosphorous and sulfur, and at least one aromatic ring. The heteroaryl radical may be a monocyclic, bicyclic, tricyclic or tetracyclic ring system, which may include fused (when fused with a cycloalkyl or heterocycloalkyl ring, the heteroaryl is bonded through an aromatic ring atom) or bridged ring systems; and the nitrogen, carbon or sulfur atoms in the heteroaryl radical may be optionally oxidized; the nitrogen atom may be optionally quaternized. In some embodiments, the heteroaryl is a 5- to 10-membered heteroaryl. In some embodiments, the heteroaryl is a 5- to 6-membered heteroaryl. Examples include, but are not limited to, azepinyl, acridinyl, benzimidazolyl, benzothiazolyl, benzindolyl, benzodioxolyl, benzofuranyl, benzooxazolyl, benzothiazolyl, benzothiadiazolyl, benzo[b][1,4]dioxepinyl, 1,4-benzodioxanyl, benzonaphthofuranyl, benzoxazolyl, benzodioxolyl, benzodioxinyl, benzopyranyl, benzopyranonyl, benzofuranyl, benzofuranonyl, benzothienyl
(benzothiophenyl), benzotriazolyl, benzo[4,6]imidazo[1,2-a]pyridinyl, carbazolyl, cinnolinyl, dibenzofuranyl, dibenzothiophenyl, furanyl, furanonyl, isothiazolyl, imidazolyl, indazolyl, indolyl, indazolyl, isoindolyl, indolinyl, isoindolinyl, isoquinolyl, indolizinyl, isoxazolyl, naphthyridinyl, oxadiazolyl, 2-oxoazepinyl, oxazolyl, oxiranyl, 1-oxidopyridinyl, 1-oxidopyrimidinyl, 1-oxidopyrazinyl, 1-oxidopyridazinyl, 1-phenyl-1H-pyrrolyl, phenazinyl, phenothiazinyl, phenoxazinyl, phthalazinyl, pteridinyl, purinyl, pyrrolyl, pyrazolyl, pyridinyl, pyrazinyl, pyrimidinyl, pyridazinyl, quinazolinyl, quinoxalinyl, quinolinyl, quinuclidinyl, isoquinolinyl, tetrahydroquinolinyl, thiazolyl, thiadiazolyl, triazolyl, tetrazolyl, triazinyl, and thiophenyl (i.e., thienyl). Unless stated otherwise specifically in the specification, a heteroaryl is optionally substituted, for example, with halogen, amino, nitrile, nitro, hydroxyl, alkyl, alkenyl, alkynyl, haloalkyl, alkoxy, aryl, cycloalkyl, heterocycloalkyl, heteroaryl, and the like. In some embodiments, a heteroaryl is optionally substituted with halogen, methyl, ethyl, -CN, - CF 3 , -OH, -OMe, -NH 2 , or -NO 2 . In some embodiments, a heteroaryl is optionally substituted with halogen, methyl, ethyl, -CN, -CF 3 , -OH, or -OMe. In some embodiments, the heteroaryl is optionally substituted with halogen.
[0043] The terms“treat,”“prevent,”“ameliorate,” and“inhibit,” as well as words stemming therefrom, as used herein, do not necessarily imply 100% or complete treatment, prevention, amelioration, or inhibition. Rather, there are varying degrees of treatment, prevention, amelioration, and inhibition of which one of ordinary skill in the art recognizes as having a potential benefit or therapeutic effect. In this respect, the disclosed methods can provide any amount of any level of treatment, prevention, amelioration, or inhibition of the disorder in a mammal. For example, a disorder, including symptoms or conditions thereof, may be reduced by, for example, about 100%, about 90%, about 80%, about 70%, about 60%, about 50%, about 40%, about 30%, about 20%, or about 10%. Furthermore, the treatment, prevention, amelioration, or inhibition provided by the methods disclosed herein can include treatment, prevention, amelioration, or inhibition of one or more conditions or symptoms of the disorder, e.g., cancer or an inflammatory disease. Also, for purposes herein,“treatment,”“prevention,” “amelioration,” or“inhibition” encompass delaying the onset of the disorder, or a symptom or condition thereof.
[0044] The terms“effective amount” or“therapeutically effective amount,” as used herein, refer to a sufficient amount of a compound disclosed herein being administered which will relieve to some extent one or more of the symptoms of the disease or condition being treated, e.g., cancer or an inflammatory disease. In some embodiments, the result is a reduction and/or alleviation of the signs, symptoms, or causes of a disease, or any other desired alteration of a biological system. For example, an“effective amount” for therapeutic uses is the amount of the composition comprising a compound disclosed herein required to provide a clinically significant decrease in disease symptoms. In some embodiments, an appropriate“effective” amount in any individual case is determined using techniques, such as a dose escalation study.
[0045] As used herein, the term“TYK2-mediated” disorders, diseases, and/or conditions as used herein means any disease or other deleterious condition in which TYK2 or a mutant thereof is known to play a role. Accordingly, another embodiment relates to treating or lessening the severity of one or more diseases in which TYK2, or a mutant thereof, is known to play a role. Such TYK2-mediated disorders include but are not limited to autoimmune disorders, inflammatory disorders, proliferative disorders, endocrine disorders, neurological disorders and disorders associated with transplantation. Compounds
[0046] Described herein are compounds that are useful in treating a TYK2-mediated disorder. In some embodiments, the TYK2-mediated disorder is an autoimmune disorder, an inflammatory disorder, a proliferative disorder, an endocrine disorder, a neurological disorder, or a disorder associated with transplantation.
[0047] Disclosed herein is a compound of Formula (I), or a pharmaceutically acceptable salt, solvate, or stereoisomer thereof:
wherein:
Ring A is optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl or optionally substituted heteroaryl;
X is CR 8 or N;
R 1 is -S(=O)R 10 , -S(=O) 2 R 10 , -S(=O) 2 NR 12 R 13 , -C(=O)R 10 , -C(=O)OR 11 , -C(=O)NR 12 R 13 , optionally substituted C 1 -C 6 alkyl, optionally substituted C 1 -C 6 heteroalkyl, optionally substituted
C 1 -C 6 haloalkyl, optionally substituted C 1 -C 6 deuteroalkyl, optionally substituted C 1 -C 6 hydroxyalkyl, optionally substituted C 1 -C 6 aminoalkyl, optionally substituted C 2 -C 6 alkenyl, optionally substituted C 2 -C 6 alkynyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl;
R 2 is hydrogen, optionally substituted C 1 -C 6 alkyl, optionally substituted C 1 -C 6 haloalkyl, or optionally substituted C 1 -C 6 deuteroalkyl;
R 3 , R 6 , and R 8 are independently hydrogen, deuterium, halogen, -CN, -OR b , -SR b , -S(=O)R a , -S(=O) 2 R a , - NO 2 , -NR c R d , -NHS(=O) 2 R a , -S(=O) 2 NR c R d , -C(=O)R a , -OC(=O)R a , -C(=O)OR b , -OC(=O)OR b , - C(=O)NR c R d , -OC(=O)NR c R d , -NR b C(=O)NR c R d , -NR b C(=O)R a , -NR b C(=O)OR b , optionally substituted C 1 -C 6 alkyl, optionally substituted C 1 -C 6 haloalkyl, optionally substituted
C 1 -C 6 deuteroalkyl, optionally substituted C 1 -C 6 hydroxyalkyl, optionally substituted
C 1 -C 6 aminoalkyl, optionally substituted C 2 -C 6 alkenyl, or optionally substituted C 2 -C 6 alkynyl;
R 4 is hydrogen, optionally substituted C 1 -C 6 alkyl, optionally substituted C 1 -C 6 heteroalkyl, optionally substituted C 1 -C 6 haloalkyl, optionally substituted C 1 -C 6 deuteroalkyl, optionally substituted C 1 -C 6 hydroxyalkyl, optionally substituted C 1 -C 6 aminoalkyl, optionally substituted C 2 -C 6 alkenyl, optionally substituted C 2 -C 6 alkynyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl;
R 5 is hydrogen, optionally substituted C 1 -C 6 alkyl, optionally substituted C 1 -C 6 haloalkyl, or optionally substituted C 1 -C 6 deuteroalkyl;
R 7 is hydrogen, optionally substituted C 1 -C 6 alkyl, optionally substituted C 1 -C 6 haloalkyl, or optionally substituted C 1 -C 6 deuteroalkyl;
each R 10 is independently optionally substituted C 1 -C 6 alkyl, optionally substituted C 1 -C 6 heteroalkyl, optionally substituted C 1 -C 6 haloalkyl, optionally substituted C 1 -C 6 deuteroalkyl, optionally substituted C 1 -C 6 hydroxyalkyl, optionally substituted C 1 -C 6 aminoalkyl, optionally substituted C 2 - C 6 alkenyl, optionally substituted C 2 -C 6 alkynyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl;
each R 11 is independently hydrogen, optionally substituted C 1 -C 6 alkyl, optionally substituted
C 1 -C 6 haloalkyl, optionally substituted C 1 -C 6 deuteroalkyl, optionally substituted C 1 -C 6 hydroxyalkyl, optionally substituted C 1 -C 6 aminoalkyl, optionally substituted C 2 -C 6 alkenyl, optionally substituted C 2 -C 6 alkynyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl;
each R 12 and R 13 is independently hydrogen, optionally substituted C 1 -C 6 alkyl, optionally substituted C 1 -C 6 haloalkyl, optionally substituted C 1 -C 6 deuteroalkyl, optionally substituted C 1 -C 6 hydroxyalkyl, optionally substituted C 1 -C 6 aminoalkyl, optionally substituted C 2 -C 6 alkenyl, optionally substituted C 2 -C 6 alkynyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl;
or R 12 and R 13 are taken together with the nitrogen atom to which they are attached to form an optionally substituted heterocycloalkyl;
each R a is independently C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl,
C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is
independently optionally substituted with one or more oxo, deuterium, halogen, -CN, -OH, -OMe, - NH 2 , -C(=O)Me, -C(=O)OH, -C(=O)OMe, C 1 -C 6 alkyl, or C 1 -C 6 haloalkyl;
each R b is independently hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is
independently optionally substituted with one or more oxo, deuterium, halogen, -CN, -OH, -OMe, - NH 2 , -C(=O)Me, -C(=O)OH, -C(=O)OMe, C 1 -C 6 alkyl, or C 1 -C 6 haloalkyl; and
each R c and R d is independently hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl,
C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more oxo, deuterium, halogen, -CN, -OH, -OMe, -NH 2 , -C(=O)Me, -C(=O)OH, -C(=O)OMe, C 1 -C 6 alkyl, or C 1 -C 6 haloalkyl;
o r Rc and Rd are taken together with the nitrogen atom to which they are attached to form a
heterocycloalkyl optionally substituted with one or more oxo, deuterium, halogen, -CN, -OH, -OMe, -NH 2 , -C(=O)Me, -C(=O)OH, -C(=O)OMe, C 1 -C 6 alkyl, or C 1 -C 6 haloalkyl.
[0048] Disclosed herein is a compound of Formula (I), or a pharmaceutically acceptable salt, solvate, or stereoisomer thereof:
wherein:
Ring A is cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; each optionally substituted with one or more R A ;
each R A is independently deuterium, halogen, -CN, -OR 15 , -SR 15 , -S(=O)R 14 , -S(=O) 2 R 14 , -NO 2 , - NR 16 R 17 , -NHS(=O) 2 R 14 , -S(=O) 2 NR 16 R 17 , -C(=O)R 14 , -OC(=O)R 14 , -C(=O)OR 15 , -OC(=O)OR 15 , - C(=O)NR 16 R 17 , -OC(=O)NR 16 R 17 , -NR 15 C(=O)NR 16 R 17 , -NR 15 C(=O)R 14 , -NR 15 C(=O)OR 15 , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more R A1 ; or two R A on the same carbon are taken together to form an oxo;
each R A1 is independently deuterium, halogen, -CN, -OR 15 , -SR 15 , -S(=O)R 14 , -S(=O) 2 R 14 , -NO 2 , - NR 16 R 17 , -NHS(=O) 2 R 14 , -S(=O) 2 NR 16 R 17 , -C(=O)R 14 , -OC(=O)R 14 , -C(=O)OR 15 , -OC(=O)OR 15 , - C(=O)NR 16 R 17 , -OC(=O)NR 16 R 17 , -NR 15 C(=O)NR 16 R 17 , -NR 15 C(=O)R 14 , -NR 15 C(=O)OR 15 , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; or two R A1 on the same carbon are taken together to form an oxo;
each R 14 is independently C 1 -C 6 alkyl, C 1 -C 6 heteroalkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl,
C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more R 14a ;
each R 14a is independently deuterium, halogen, -CN, -OR b , -SR b , -S(=O)R a , -S(=O) 2 R a , -NO 2 , -NR c R d , - NHS(=O) 2 R a , -S(=O) 2 NR c R d , -C(=O)R a , -OC(=O)R a , -C(=O)OR b , -OC(=O)OR b , -C(=O)NR c R d , - OC(=O)NR c R d , -NR b C(=O)NR c R d , -NR b C(=O)R a , -NR b C(=O)OR b , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; or two R 14a on the same carbon are taken together to form an oxo;
e ach R15 is independently hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is
independently optionally substituted with one or more R 15a ;
each R 15a is independently deuterium, halogen, -CN, -OR b , -SR b , -S(=O)R a , -S(=O) 2 R a , -NO 2 , -NR c R d , - NHS(=O) 2 R a , -S(=O) 2 NR c R d , -C(=O)R a , -OC(=O)R a , -C(=O)OR b , -OC(=O)OR b , -C(=O)NR c R d , - OC(=O)NR c R d , -NR b C(=O)NR c R d , -NR b C(=O)R a , -NR b C(=O)OR b , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; or two R 15a on the same carbon are taken together to form an oxo;
each R 16 and R 17 is independently hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl,
C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more R 16a ;
or R 16 and R 17 are taken together with the nitrogen atom to which they are attached to form a
heterocycloalkyl optionally substituted with one or more R 16b ;
each R 16a is independently deuterium, halogen, -CN, -OR b , -SR b , -S(=O)R a , -S(=O) 2 R a , -NO 2 , -NR c R d , - NHS(=O) 2 R a , -S(=O) 2 NR c R d , -C(=O)R a , -OC(=O)R a , -C(=O)OR b , -OC(=O)OR b , -C(=O)NR c R d , - OC(=O)NR c R d , -NR b C(=O)NR c R d , -NR b C(=O)R a , -NR b C(=O)OR b , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl, or two R 16a on the same carbon are taken together to form an oxo;
each R 16b is independently deuterium, halogen, -CN, -OR b , -SR b , -S(=O)R a , -S(=O) 2 R a , -NO 2 , -NR c R d , - NHS(=O) 2 R a , -S(=O) 2 NR c R d , -C(=O)R a , -OC(=O)R a , -C(=O)OR b , -OC(=O)OR b , -C(=O)NR c R d , - OC(=O)NR c R d , -NR b C(=O)NR c R d , -NR b C(=O)R a , -NR b C(=O)OR b , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; or two R 16b on the same carbon are taken together to form an oxo;
X is CR 8 or N;
R 1 is -S(=O)R 10 , -S(=O) 2 R 10 , -S(=O) 2 NR 12 R 13 , -C(=O)R 10 , -C(=O)OR 11 , -C(=O)NR 12 R 13 , C 1 -C 6 alkyl, C 1 -C 6 heteroalkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C2- C6alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more R 1a ; each R 1a is independently deuterium, halogen, -CN, -OR b , -SR b , -S(=O)R a , -S(=O) 2 R a , -NO 2 , -NR c R d , - NHS(=O) 2 R a , -S(=O) 2 NR c R d , -C(=O)R a , -OC(=O)R a , -C(=O)OR b , -OC(=O)OR b , -C(=O)NR c R d , - O C(=O)NRcRd, -NRbC(=O)NRcRd, -NRbC(=O)Ra, -NRbC(=O)ORb, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, alkenyl, alkynyl, cycloalkyl,
heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more R 1b ; or two R 1a on the same carbon are taken together to form an oxo;
each R 1b is independently deuterium, halogen, -CN, -OR b , -SR b , -S(=O)R a , -S(=O) 2 R a , -NO 2 , -NR c R d , - NHS(=O) 2 R a , -S(=O) 2 NR c R d , -C(=O)R a , -OC(=O)R a , -C(=O)OR b , -OC(=O)OR b , -C(=O)NR c R d , - OC(=O)NR c R d , -NR b C(=O)NR c R d , -NR b C(=O)R a , -NR b C(=O)OR b , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; or two R 1b on the same carbon are taken together to form an oxo;
R 2 is hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, or C 1 -C 6 deuteroalkyl;
R 3 , R 6 , and R 8 are independently hydrogen, deuterium, halogen, -CN, -OR b , -SR b , -S(=O)R a , -S(=O) 2 R a , - NO 2 , -NR c R d , -NHS(=O) 2 R a , -S(=O) 2 NR c R d , -C(=O)R a , -OC(=O)R a , -C(=O)OR b , -OC(=O)OR b , - C(=O)NR c R d , -OC(=O)NR c R d , -NR b C(=O)NR c R d , -NR b C(=O)R a , -NR b C(=O)OR b , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, or C2- C6alkynyl;
R 4 is hydrogen, C 1 -C 6 alkyl, C 1 -C 6 heteroalkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl are optionally substituted with one or more R 4a ;
each R 4a is independently deuterium, halogen, -CN, -OR b , -NR c R d , -C(=O)R a , -C(=O)OR b , -C(=O)NR c R d , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; or two R 4a on the same carbon are taken together to form an oxo;
R 5 is hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, or C 1 -C 6 deuteroalkyl;
R 7 is hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, or C 1 -C 6 deuteroalkyl;
each R 10 is independently C 1 -C 6 alkyl, C 1 -C 6 heteroalkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl,
C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more R 10a ;
each R 10a is independently deuterium, halogen, -CN, -OR b , -SR b , -S(=O)R a , -S(=O) 2 R a , -NO 2 , -NR c R d , - NHS(=O) 2 R a , -S(=O) 2 NR c R d , -C(=O)R a , -OC(=O)R a , -C(=O)OR b , -OC(=O)OR b , -C(=O)NR c R d , - OC(=O)NR c R d , -NR b C(=O)NR c R d , -NR b C(=O)R a , -NR b C(=O)OR b , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; or two R 10a on the same carbon are taken together to form an oxo;
e ach R11 is independently hydrogen, C 1 -C 6 alkyl, C 1 -C 6 heteroalkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more R 11a ;
each R 11a is independently hydrogen, deuterium, halogen, -CN, -OR b , -SR b , -S(=O)R a , -S(=O) 2 R a , -NO 2 , - NR c R d , -NHS(=O) 2 R a , -S(=O) 2 NR c R d , -C(=O)R a , -OC(=O)R a , -C(=O)OR b , -OC(=O)OR b , - C(=O)NR c R d , -OC(=O)NR c R d , -NR b C(=O)NR c R d , -NR b C(=O)R a , -NR b C(=O)OR b , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 - C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; or two R 11a on the same carbon are taken together to form an oxo
each R 12 and R 13 is independently hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl,
C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more R 12a ;
each R 12a is independently deuterium, halogen, -CN, -OR b , -SR b , -S(=O)R a , -S(=O) 2 R a , -NO 2 , -NR c R d , - NHS(=O) 2 R a , -S(=O) 2 NR c R d , -C(=O)R a , -OC(=O)R a , -C(=O)OR b , -OC(=O)OR b , -C(=O)NR c R d , - OC(=O)NR c R d , -NR b C(=O)NR c R d , -NR b C(=O)R a , -NR b C(=O)OR b , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; or two R 12a on the same carbon are taken together to form an oxo;
or R 12 and R 13 are taken together with the nitrogen atom to which they are attached to form a
heterocycloalkyl optionally substituted with one or more R 12b .
each R 12b is independently deuterium, halogen, -CN, -OR b , -SR b , -S(=O)R a , -S(=O) 2 R a , -NO 2 , -NR c R d , - NHS(=O) 2 R a , -S(=O) 2 NR c R d , -C(=O)R a , -OC(=O)R a , -C(=O)OR b , -OC(=O)OR b , -C(=O)NR c R d , - OC(=O)NR c R d , -NR b C(=O)NR c R d , -NR b C(=O)R a , -NR b C(=O)OR b , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; or two R 12b on the same carbon are taken together to form an oxo;
each R a is independently C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl,
C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is
independently optionally substituted with one or more oxo, deuterium, halogen, -CN, -OH, -OMe, - NH 2 , -C(=O)Me, -C(=O)OH, -C(=O)OMe, C 1 -C 6 alkyl, or C 1 -C 6 haloalkyl;
each R b is independently hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is
independently optionally substituted with one or more oxo, deuterium, halogen, -CN, -OH, -OMe, - N H 2 , -C(=O)Me, -C(=O)OH, -C(=O)OMe, C 1 -C 6 alkyl, or C 1 -C 6 haloalkyl; and
each R c and R d is independently hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl,
C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more oxo, deuterium, halogen, -CN, -OH, -OMe, -NH 2 , -C(=O)Me, -C(=O)OH, -C(=O)OMe, C 1 -C 6 alkyl, or C 1 -C 6 haloalkyl;
or R c and R d are taken together with the nitrogen atom to which they are attached to form a
heterocycloalkyl optionally substituted with one or more oxo, deuterium, halogen, -CN, -OH, -OMe, -NH 2 , -C(=O)Me, -C(=O)OH, -C(=O)OMe, C 1 -C 6 alkyl, or C 1 -C 6 haloalkyl.
[0049] In some embodiments of a compound of Formula (I), Ring A is cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; each optionally substituted with one or more R A . In some embodiments of a compound of Formula (I), Ring A is heterocycloalkyl, aryl, or heteroaryl; each optionally substituted with one or more R A . In some embodiments of a compound of Formula (I), Ring A is heterocycloalkyl optionally substituted with one or more R A . In some embodiments of a compound of Formula (I), Ring A is aryl or heteroaryl; each optionally substituted with one or more R A . In some embodiments of a compound of Formula (I), Ring A is aryl optionally substituted with one or more R A . In some embodiments of a compound of Formula (I), Ring A is phenyl, pyridyl, pyrimidyl, pyrazinyl, or pyridazinyl, each optionally substituted with one or more R A .
[0050] In some embodiments of a compound of Formula (I), each R A is independently deuterium, halogen, -CN, -OR 15 , -SR 15 , -S(=O)R 14 , -S(=O) 2 R 14 , -NO 2 , -NR 16 R 17 , -NHS(=O) 2 R 14 , -S(=O) 2 NR 16 R 17 , - C(=O)R 14 , -OC(=O)R 14 , -C(=O)OR 15 , -OC(=O)OR 15 , -C(=O)NR 16 R 17 , -OC(=O)NR 16 R 17 , - NR 15 C(=O)NR 16 R 17 , -NR 15 C(=O)R 14 , -NR 15 C(=O)OR 15 , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more R A1 ; or two R A on the same carbon are taken together to form an oxo.
[0051] In some embodiments of a compound of Formula (I), each R A is independently deuterium, halogen, -CN, -OR 15 , -NR 16 R 17 , -C(=O)R 14 , -C(=O)OR 15 , -C(=O)NR 16 R 17 , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more R A1 ; or two R A on the same carbon are taken together to form an oxo.
[ 0052] In some embodiments of a compound of Formula (I), each R A is independently deuterium, halogen, -CN, -OR 15 , -NR 16 R 17 , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, cycloalkyl,
heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more R A1 ; or two R A on the same carbon are taken together to form an oxo.
[0053] In some embodiments of a compound of Formula (I), each R A1 is independently deuterium, halogen, -CN, -OR 15 , -SR 15 , -S(=O)R 14 , -S(=O) 2 R 14 , -NO 2 , -NR 16 R 17 , -NHS(=O) 2 R 14 , -S(=O) 2 NR 16 R 17 , - C(=O)R 14 , -OC(=O)R 14 , -C(=O)OR 15 , -OC(=O)OR 15 , -C(=O)NR 16 R 17 , -OC(=O)NR 16 R 17 , - NR 15 C(=O)NR 16 R 17 , -NR 15 C(=O)R 14 , -NR 15 C(=O)OR 15 , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; or two R A1 on the same carbon are taken together to form an oxo.
[0054] In some embodiments of a compound of Formula (I), each R A1 is independently deuterium, halogen, -CN, -OR 15 , -NR 16 R 17 , -C(=O)R 14 , -C(=O)OR 15 , -C(=O)NR 16 R 17 , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; or two R A1 on the same carbon are taken together to form an oxo.
[0055] In some embodiments of a compound of Formula (I), each R A1 is independently deuterium, halogen, -CN, -OR 15 , -NR 16 R 17 , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, cycloalkyl, or heterocycloalkyl; or two R A1 on the same carbon are taken together to form an oxo.
[0056] In some embodiments of a compound of Formula (I), each R 14 is independently C 1 -C 6 alkyl, C 1 -C 6 heteroalkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C2- C6alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more R 14a .
[0057] In some embodiments of a compound of Formula (I), each R 14 is independently C 1 -C 6 alkyl, C 1 -C 6 heteroalkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more R 14a .
[ 0058] In some embodiments of a compound of Formula (I), each R 14 is independently C 1 -C 6 alkyl, C 1 -C 6 heteroalkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, cycloalkyl, or heterocycloalkyl; wherein each alkyl, cycloalkyl, and heterocycloalkyl is independently optionally substituted with one or more R 14a .
[0059] In some embodiments of a compound of Formula (I), each R 14a is independently deuterium, halogen, -CN, -OR b , -SR b , -S(=O)R a , -S(=O) 2 R a , -NO 2 , -NR c R d , -NHS(=O) 2 R a , -S(=O) 2 NR c R d , -C(=O)R a , -OC(=O)R a , -C(=O)OR b , -OC(=O)OR b , -C(=O)NR c R d , -OC(=O)NR c R d , -NR b C(=O)NR c R d , - NR b C(=O)R a , -NR b C(=O)OR b , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; or two R 14a on the same carbon are taken together to form an oxo.
[0060] In some embodiments of a compound of Formula (I), each R 14a is independently deuterium, halogen, -CN, -OR b , -NR c R d , -C(=O)R a , -C(=O)OR b , -C(=O)NR c R d , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; or two R 14a on the same carbon are taken together to form an oxo. [0061] In some embodiments of a compound of Formula (I), each R 15 is independently hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 - C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more R 15a .
[0062] In some embodiments of a compound of Formula (I), each R 15 is independently hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more R 15a .
[0063] In some embodiments of a compound of Formula (I), each R 15 is independently hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, cycloalkyl, or heterocycloalkyl; wherein each alkyl, cycloalkyl, or heterocycloalkyl is independently optionally substituted with one or more R 15a .
[0064] In some embodiments of a compound of Formula (I), each R 15a is independently deuterium, halogen, -CN, -OR b , -SR b , -S(=O)R a , -S(=O) 2 R a , -NO 2 , -NR c R d , -NHS(=O) 2 R a , -S(=O) 2 NR c R d , -C(=O)R a , -OC(=O)R a , -C(=O)OR b , -OC(=O)OR b , -C(=O)NR c R d , -OC(=O)NR c R d , -NR b C(=O)NR c R d , - NR b C(=O)R a , -NR b C(=O)OR b , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; or two R 15a on the same carbon are taken together to form an oxo.
[0065] In some embodiments of a compound of Formula (I), each R 15a is independently deuterium, halogen, -CN, -OR b , -NR c R d , -C(=O)R a , -C(=O)OR b , -C(=O)NR c R d , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; or two R 15a on the same carbon are taken together to form an oxo.
[0066] In some embodiments of a compound of Formula (I), each R 15a is independently deuterium, halogen, -CN, -OR b , -NR c R d , -C(=O)R a , -C(=O)OR b , -C(=O)NR c R d , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, cycloalkyl, or heterocycloalkyl; or two R 15a on the same carbon are taken together to form an oxo.
[0067] In some embodiments of a compound of Formula (I), each R 16 and R 17 is independently hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 - C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more R 16a .
[0068] In some embodiments of a compound of Formula (I), each R 16 and R 17 is independently hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more R 16a .
[0069] In some embodiments of a compound of Formula (I), each R 16 and R 17 is independently hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, cycloalkyl, or heterocycloalkyl is independently optionally substituted with one or more R 16a .
[0070] In some embodiments of a compound of Formula (I), each R 16a is independently deuterium, halogen, -CN, -OR b , -SR b , -S(=O)R a , -S(=O) 2 R a , -NO 2 , -NR c R d , -NHS(=O) 2 R a , -S(=O) 2 NR c R d , -C(=O)R a , -OC(=O)R a , -C(=O)OR b , -OC(=O)OR b , -C(=O)NR c R d , -OC(=O)NR c R d , -NR b C(=O)NR c R d , - NR b C(=O)R a , -NR b C(=O)OR b , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl, or two R 16a on the same carbon are taken together to form an oxo.
[0071] In some embodiments of a compound of Formula (I), each R 16a is independently deuterium, halogen, -CN, -OR b , -NR c R d , -C(=O)R a , -C(=O)OR b , -C(=O)NR c R d , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl, or two R 16a on the same carbon are taken together to form an oxo.
[0072] In some embodiments of a compound of Formula (I), each R 16a is independently deuterium, halogen, -CN, -OR b , -NR c R d , -C(=O)R a , -C(=O)OR b , -C(=O)NR c R d , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, cycloalkyl, or heterocycloalkyl, or two R 16a on the same carbon are taken together to form an oxo.
[0073] In some embodiments of a compound of Formula (I), R 16 and R 17 are taken together with the nitrogen atom to which they are attached to form a heterocycloalkyl optionally substituted with one or more R 16b .
[0074] In some embodiments of a compound of Formula (I), each R 16b is independently deuterium, halogen, -CN, -OR b , -SR b , -S(=O)R a , -S(=O) 2 R a , -NO 2 , -NR c R d , -NHS(=O) 2 R a , -S(=O) 2 NR c R d , -C(=O)R a , -OC(=O)R a , -C(=O)OR b , -OC(=O)OR b , -C(=O)NR c R d , -OC(=O)NR c R d , -NR b C(=O)NR c R d , - NR b C(=O)R a , -NR b C(=O)OR b , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; or two R 16b on the same carbon are taken together to form an oxo.
[ 0075] In some embodiments of a compound of Formula (I), each R 16b is independently deuterium, halogen, -CN, -OR b , -NR c R d , -C(=O)R a , -C(=O)OR b , -C(=O)NR c R d , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; or two R 16b on the same carbon are taken together to form an oxo.
[0076] In some embodiments of a compound of Formula (I), each R 16b is independently deuterium, halogen, -CN, -OR b , -NR c R d , -C(=O)R a , -C(=O)OR b , -C(=O)NR c R d , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, cycloalkyl, or heterocycloalkyl; or two R 16b on the same carbon are taken together to form an oxo.
[0077] In some embodiments of a compound of Formula (I), X is N. In some embodiments of a compound of Formula (I), X is CR 8 .
[0078] In some embodiments of a compound of Formula (I), R 1 is -S(=O)R 10 , -S(=O) 2 R 10 , - S(=O) 2 NR 12 R 13 , -C(=O)R 10 , -C(=O)OR 11 , -C(=O)NR 12 R 13 , C 1 -C 6 alkyl, C 1 -C 6 heteroalkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more R1a.
[0079] In some embodiments of a compound of Formula (I), R 1 is -C(=O)R 10 , C 1 -C 6 alkyl,
C 1 -C 6 heteroalkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more R 1a . In some embodiments of a compound of Formula (I), R 1 is -C(=O)R 10 . In some embodiments of a compound of Formula (I), R 1 is C 1 -C 6 alkyl, C 1 -C 6 heteroalkyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more R 1a . In some embodiments of a compound of Formula (I), R 1 is heteroaryl optionally substituted with one or more R 1a . In some embodiments of a compound of Formula (I), R 1 is -C(=O)R 10 or heteroaryl optionally substituted with one or more R 1a .
[0080] In some embodiments of a compound of Formula (I), each R 1a is independently deuterium, halogen, -CN, -OR b , -SR b , -S(=O)R a , -S(=O) 2 R a , -NO 2 , -NR c R d , -NHS(=O) 2 R a , -S(=O) 2 NR c R d , -C(=O)R a , -OC(=O)R a , -C(=O)OR b , -OC(=O)OR b , -C(=O)NR c R d , -OC(=O)NR c R d , -NR b C(=O)NR c R d , - NR b C(=O)R a , -NR b C(=O)OR b , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more R 1b ; or two R 1a on the same carbon are taken together to form an oxo.
[0081] In some embodiments of a compound of Formula (I), each R 1a is independently deuterium, halogen, -CN, -OR b , -NR c R d , -C(=O)R a , -C(=O)OR b , -C(=O)NR c R d , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more R 1b ; or two R 1a on the same carbon are taken together to form an oxo.
[0082] In some embodiments of a compound of Formula (I), each R 1a is independently deuterium, halogen, -CN, -OR b , -NR c R d , -C(=O)R a , -C(=O)OR b , -C(=O)NR c R d , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, cycloalkyl, or heterocycloalkyl; wherein each alkyl, cycloalkyl, and heterocycloalkyl is independently optionally substituted with one or more R 1b ; or two R 1a on the same carbon are taken together to form an oxo.
[0083] In some embodiments of a compound of Formula (I), each R 1b is independently deuterium, halogen, -CN, -OR b , -SR b , -S(=O)R a , -S(=O) 2 R a , -NO 2 , -NR c R d , -NHS(=O) 2 R a , -S(=O) 2 NR c R d , -C(=O)R a , -OC(=O)R a , -C(=O)OR b , -OC(=O)OR b , -C(=O)NR c R d , -OC(=O)NR c R d , -NR b C(=O)NR c R d , - NR b C(=O)R a , -NR b C(=O)OR b , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; or two R 1b on the same carbon are taken together to form an oxo. [0084] In some embodiments of a compound of Formula (I), each R 1b is independently deuterium, halogen, -CN, -OR b , -NR c R d , -C(=O)R a , -C(=O)OR b , -C(=O)NR c R d , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; or two R 1b on the same carbon are taken together to form an oxo.
[0085] In some embodiments of a compound of Formula (I), each R 1b is independently deuterium, halogen, -CN, -OR b , -NR c R d , -C(=O)R a , -C(=O)OR b , -C(=O)NR c R d , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, cycloalkyl, or heterocycloalkyl; or two R 1b on the same carbon are taken together to form an oxo.
[0086] In some embodiments of a compound of Formula (I), each R 10 is independently C 1 -C 6 alkyl, C 1 -C 6 heteroalkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 - C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more R 10a .
[0087] In some embodiments of a compound of Formula (I), each R 10 is independently C 1 -C 6 alkyl, C 1 -C 6 heteroalkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more R 10a .
[0088] In some embodiments of a compound of Formula (I), each R 10 is independently C 1 -C 6 alkyl, C 1 -C 6 heteroalkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, cycloalkyl, or heterocycloalkyl; wherein each alkyl, cycloalkyl, or heterocycloalkyl is independently optionally substituted with one or more R 10a .
[0089] In some embodiments of a compound of Formula (I), each R 10a is independently deuterium, halogen, -CN, -OR b , -SR b , -S(=O)R a , -S(=O) 2 R a , -NO 2 , -NR c R d , -NHS(=O) 2 R a , -S(=O) 2 NR c R d , -C(=O)R a , -OC(=O)R a , -C(=O)OR b , -OC(=O)OR b , -C(=O)NR c R d , -OC(=O)NR c R d , -NR b C(=O)NR c R d , - NR b C(=O)R a , -NR b C(=O)OR b , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; or two R 10a on the same carbon are taken together to form an oxo.
[0090] In some embodiments of a compound of Formula (I), each R 10a is independently deuterium, halogen, -CN, -OR b , -NO 2 , -NR c R d , -C(=O)R a , -C(=O)OR b , -C(=O)NR c R d , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; or two R 10a on the same carbon are taken together to form an oxo.
[0091] In some embodiments of a compound of Formula (I), each R 10a is independently deuterium, halogen, -CN, -OR b , -NO 2 , -NR c R d , -C(=O)R a , -C(=O)OR b , -C(=O)NR c R d , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, cycloalkyl, or heterocycloalkyl; or two R 10a on the same carbon are taken together to form an oxo.
[0092] In some embodiments of a compound of Formula (I), each R 11 is independently hydrogen, C 1 -C 6 alkyl, C 1 -C 6 heteroalkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more R11a.
[0093] In some embodiments of a compound of Formula (I), each R 11 is independently hydrogen, C 1 -C 6 alkyl, C 1 -C 6 heteroalkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more R 11a .
[0094] In some embodiments of a compound of Formula (I), each R 11 is independently hydrogen, C 1 -C 6 alkyl, C 1 -C 6 heteroalkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, cycloalkyl, or heterocycloalkyl;
wherein each alkyl, cycloalkyl, and heterocycloalkyl is independently optionally substituted with one or more R 11a .
[0095] In some embodiments of a compound of Formula (I), each R 11a is independently hydrogen, deuterium, halogen, -CN, -OR b , -SR b , -S(=O)R a , -S(=O) 2 R a , -NO 2 , -NR c R d , -NHS(=O) 2 R a , - S(=O) 2 NR c R d , -C(=O)R a , -OC(=O)R a , -C(=O)OR b , -OC(=O)OR b , -C(=O)NR c R d , -OC(=O)NR c R d , - NR b C(=O)NR c R d , -NR b C(=O)R a , -NR b C(=O)OR b , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; or two R 11a on the same carbon are taken together to form an oxo.
[0096] In some embodiments of a compound of Formula (I), each R 11a is independently hydrogen, deuterium, halogen, -CN, -OR b , -NR c R d , -C(=O)R a , -C(=O)OR b , -C(=O)NR c R d , C 1 -C 6 alkyl,
C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; or two R 11a on the same carbon are taken together to form an oxo.
[0097] In some embodiments of a compound of Formula (I), each R 11a is independently hydrogen, deuterium, halogen, -CN, -OR b , -NR c R d , -C(=O)R a , -C(=O)OR b , -C(=O)NR c R d , C 1 -C 6 alkyl,
C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, cycloalkyl, or heterocycloalkyl; or two R 11a on the same carbon are taken together to form an oxo.
[ 0098] In some embodiments of a compound of Formula (I), each R 12 and R 13 is independently hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C2- C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more R 12a .
[0099] In some embodiments of a compound of Formula (I), each R 12 and R 13 is independently hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more R 12a .
[00100] In some embodiments of a compound of Formula (I), each R 12 and R 13 is independently hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, cycloalkyl, or heterocycloalkyl; wherein each alkyl, cycloalkyl, and heterocycloalkyl is independently optionally substituted with one or more R 12a . [00101] In some embodiments of a compound of Formula (I), each R 12a is independently deuterium, halogen, -CN, -OR b , -SR b , -S(=O)R a , -S(=O) 2 R a , -NO 2 , -NR c R d , -NHS(=O) 2 R a , -S(=O) 2 NR c R d , -C(=O)R a , -OC(=O)R a , -C(=O)OR b , -OC(=O)OR b , -C(=O)NR c R d , -OC(=O)NR c R d , -NR b C(=O)NR c R d , - NR b C(=O)R a , -NR b C(=O)OR b , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; or two R 12a on the same carbon are taken together to form an oxo.
[00102] In some embodiments of a compound of Formula (I), each R 12a is independently deuterium, halogen, -CN, -OR b , -NR c R d , -C(=O)R a , -C(=O)OR b , -C(=O)NR c R d , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; or two R 12a on the same carbon are taken together to form an oxo.
[00103] In some embodiments of a compound of Formula (I), each R 12a is independently deuterium, halogen, -CN, -OR b , -NR c R d , -C(=O)R a , -C(=O)OR b , -C(=O)NR c R d , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, cycloalkyl, or heterocycloalkyl; or two R 12a on the same carbon are taken together to form an oxo.
[00104] In some embodiments of a compound of Formula (I), R 12 and R 13 are taken together with the nitrogen atom to which they are attached to form a heterocycloalkyl optionally substituted with one or more R 12b .
[00105] In some embodiments of a compound of Formula (I), each R 12b is independently deuterium, halogen, -CN, -OR b , -SR b , -S(=O)R a , -S(=O) 2 R a , -NO 2 , -NR c R d , -NHS(=O) 2 R a , -S(=O) 2 NR c R d , -C(=O)R a , -OC(=O)R a , -C(=O)OR b , -OC(=O)OR b , -C(=O)NR c R d , -OC(=O)NR c R d , -NR b C(=O)NR c R d , - NR b C(=O)R a , -NR b C(=O)OR b , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; or two R 12b on the same carbon are taken together to form an oxo.
[ 00106] In some embodiments of a compound of Formula (I), each R 12b is independently deuterium, halogen, -CN, -OR b , -NR c R d , -C(=O)R a , -C(=O)OR b , -C(=O)NR c R d , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; or two R 12b on the same carbon are taken together to form an oxo.
[00107] In some embodiments of a compound of Formula (I), each R 12b is independently deuterium, halogen, -CN, -OR b , -NR c R d , -C(=O)R a , -C(=O)OR b , -C(=O)NR c R d , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, cycloalkyl, or heterocycloalkyl; or two R 12b on the same carbon are taken together to form an oxo.
[00108] In some embodiments of a compound of Formula (I), R 2 is hydrogen, C 1 -C 6 alkyl,
C 1 -C 6 haloalkyl, or C 1 -C 6 deuteroalkyl. In some embodiments of a compound of Formula (I), R 2 is hydrogen or C 1 -C 6 alkyl. In some embodiments of a compound of Formula (I), R 2 is hydrogen.
[00109] In some embodiments of a compound of Formula (I), R 3 , R 6 , and R 8 are independently hydrogen, deuterium, halogen, -CN, -OR b , -SR b , -S(=O)R a , -S(=O) 2 R a , -NO 2 , -NR c R d , -NHS(=O) 2 R a , - S(=O) 2 NR c R d , -C(=O)R a , -OC(=O)R a , -C(=O)OR b , -OC(=O)OR b , -C(=O)NR c R d , -OC(=O)NR c R d , - NR b C(=O)NR c R d , -NR b C(=O)R a , -NR b C(=O)OR b , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, or C 2 -C 6 alkynyl. In some embodiments of a compound of Formula (I), R3, R6, and R8 are independently hydrogen, deuterium, halogen, -CN, -ORb, - NR c R d , -C(=O)NR c R d , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, or C 1 -C 6 deuteroalkyl. In some embodiments of a compound of Formula (I), R 3 , R 6 , and R 8 are independently hydrogen, deuterium, halogen, or C 1 -C 6 alkyl. In some embodiments of a compound of Formula (I), R 3 , R 6 , and R 8 are hydrogen.
[00110] In some embodiments of a compound of Formula (I), R 4 is hydrogen, C 1 -C 6 alkyl,
C 1 -C 6 heteroalkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 - C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl are optionally substituted with one or more R 4a .
[00111] In some embodiments of a compound of Formula (I), R 4 is hydrogen, C 1 -C 6 alkyl,
C 1 -C 6 heteroalkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl are optionally substituted with one or more R 4a .
[00112] In some embodiments of a compound of Formula (I), R 4 is hydrogen, C 1 -C 6 alkyl,
C 1 -C 6 heteroalkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, cycloalkyl, or heterocycloalkyl; wherein each alkyl, cycloalkyl, or heterocycloalkyl are optionally substituted with one or more R 4a .
[00113] In some embodiments of a compound of Formula (I), R 4 is hydrogen, C 1 -C 6 alkyl,
C 1 -C 6 haloalkyl, or C 1 -C 6 deuteroalkyl.
[00114] In some embodiments of a compound of Formula (I), R 4 is hydrogen, C 1 -C 6 alkyl, or
C 1 -C 6 deuteroalkyl.
[00115] In some embodiments of a compound of Formula (I), R 4 is hydrogen or C 1 -C 6 alkyl optionally substituted with one or more R 4a . In some embodiments of a compound of Formula (I), R 4 is C 1 -C 6 alkyl optionally substituted with one or more R 4a .
[00116] In some embodiments of a compound of Formula (I), R 4 is hydrogen or C 1 -C 6 alkyl. In some embodiments of a compound of Formula (I), R 4 is C 1 -C 6 alkyl.
[00117] In some embodiments of a compound of Formula (I), each R 4a is independently deuterium, halogen, -CN, -OR b , -NR c R d , -C(=O)R a , -C(=O)OR b , -C(=O)NR c R d , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; or two R 4a on the same carbon are taken together to form an oxo.
[00118] In some embodiments of a compound of Formula (I), each R 4a is independently deuterium, halogen, -CN, -OR b , -NR c R d , -C(=O)R a , -C(=O)OR b , -C(=O)NR c R d , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, cycloalkyl, or heterocycloalkyl; or two R 4a on the same carbon are taken together to form an oxo. [00119] In some embodiments of a compound of Formula (I), each R 4a is independently deuterium, halogen, -CN, -OR b , -NR c R d , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, cycloalkyl, or
heterocycloalkyl; or two R 4a on the same carbon are taken together to form an oxo.
[00120] In some embodiments of a compound of Formula (I), R 5 is hydrogen, C 1 -C 6 alkyl,
C 1 -C 6 haloalkyl, or C 1 -C 6 deuteroalkyl. In some embodiments of a compound of Formula (I), R 5 is hydrogen or C 1 -C 6 alkyl. In some embodiments of a compound of Formula (I), R 5 is hydrogen.
[00121] In some embodiments of a compound of Formula (I), R 7 is hydrogen, C 1 -C 6 alkyl,
C 1 -C 6 haloalkyl, or C 1 -C 6 deuteroalkyl. In some embodiments of a compound of Formula (I), R 7 is hydrogen or C 1 -C 6 alkyl. In some embodiments of a compound of Formula (I), R 7 is hydrogen.
[00122] In some embodiments of a compound described above, each R a is independently C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, or cycloalkyl; wherein each alkyl and cycloalkyl is independently optionally substituted with one or more oxo, deuterium, halogen, -CN, -OH, -OMe, -NH 2 , -C(=O)Me, - C(=O)OH, -C(=O)OMe, C 1 -C 6 alkyl, or C 1 -C 6 haloalkyl. In some embodiments of a compound described above, each R a is independently C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, or C 1 -C 6 deuteroalkyl. In some embodiments of a compound described above, each R a is independently C 1 -C 6 alkyl.
[00123] In some embodiments of a compound described above, each R b is independently hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, or cycloalkyl; wherein each alkyl and cycloalkyl is independently optionally substituted with one or more oxo, deuterium, halogen, -CN, -OH, -OMe, -NH 2 , -C(=O)Me, -C(=O)OH, -C(=O)OMe, C 1 -C 6 alkyl, or C 1 -C 6 haloalkyl. In some embodiments of a compound described above, each R b is independently hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, or
C 1 -C 6 deuteroalkyl. In some embodiments of a compound described above, each R b is independently hydrogen or C 1 -C 6 alkyl. In some embodiments of a compound described above, each R b is hydrogen. In some embodiments of a compound described above, each R b is independently C 1 -C 6 alkyl.
[ 00124] In some embodiments of a compound described above, each R c and R d is independently hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, or cycloalkyl; wherein each alkyl and cycloalkyl is independently optionally substituted with one or more oxo, deuterium, halogen, -CN, -OH, - OMe, -NH 2 , -C(=O)Me, -C(=O)OH, -C(=O)OMe, C 1 -C 6 alkyl, or C 1 -C 6 haloalkyl. In some embodiments of a compound described above, each R c and R d is independently hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, or C 1 -C 6 deuteroalkyl. In some embodiments of a compound described above, each R c and R d is independently hydrogen or C 1 -C 6 alkyl. In some embodiments of a compound described above, each R c and R d is hydrogen. In some embodiments of a compound described above, each R c and R d is independently C 1 -C 6 alkyl.
[00125] In some embodiments of a compound of Formula (I), each Ring A, R A , R 14 , R 15 , R 16 , R 17 , R 1 , R 4 , R 10 , R 11 , R 12 , R 13 , R a , R b , R c , and R d is independently optionally substituted with one, two, three, or four substituents as defined herein. In some embodiments of a compound of Formula (I), each Ring A, R A , R 14 , R 15 , R 16 , R 17 , R 1 , R 4 , R 10 , R 11 , R 12 , R 13 , R a , R b , R c , and R d is independently optionally substituted with one, two, or three substituents as defined herein. In some embodiments of a compound of Formula (I), each Ring A, R A , R 14 , R 15 , R 16 , R 17 , R 1 , R 4 , R 10 , R 11 , R 12 , R 13 , R a , R b , R c , and R d is independently optionally substituted with one or two substituents as defined herein. In some embodiments of a compound of Formula (I), each Ring A, R A , R 14 , R 15 , R 16 , R 17 , R 1 , R 4, R 10, R 11 , R 12 , R 13 , R a , R b , R c , and R d is independently optionally substituted with one substituent as defined herein. [00126] Also disclosed herein is a compound of Formula (II), or a pharmaceutically acceptable salt, solvate, or stereoisomer thereof:
wherein:
L is a 4-10 atom optionally substituted linker;
Ring A is optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl or optionally substituted heteroaryl;
is a single bond or a double bond;
X 1 and X 2 are -N- or -C=; provided that one of X 1 or X 2 is -N- and the other is -C=;
Y 8 is CR 8 or N;
Y 6 is CR 6 or N;
Y 3 is CR 3 or N;
Y 9 is CR 9 or N;
R 3 , R 6 , R 8 , and R 9 are independently hydrogen, deuterium, halogen, -CN, -OR b , -SR b , -S(=O)R a , - S(=O) 2 R a , -NO 2 , -NR c R d , -NHS(=O) 2 R a , -S(=O) 2 NR c R d , -C(=O)R a , -OC(=O)R a , -C(=O)OR b , - OC(=O)OR b , -C(=O)NR c R d , -OC(=O)NR c R d , -NR b C(=O)NR c R d , -NR b C(=O)R a , -NR b C(=O)OR b , optionally substituted C 1 -C 6 alkyl, optionally substituted C 1 -C 6 haloalkyl, optionally substituted C 1 -C 6 deuteroalkyl, optionally substituted C 1 -C 6 hydroxyalkyl, optionally substituted
C 1 -C 6 aminoalkyl, optionally substituted C 2 -C 6 alkenyl, or optionally substituted C 2 -C 6 alkynyl;
R 4 is hydrogen, optionally substituted C 1 -C 6 alkyl, optionally substituted C 1 -C 6 heteroalkyl, optionally substituted C 1 -C 6 haloalkyl, optionally substituted C 1 -C 6 deuteroalkyl, optionally substituted
C 1 -C 6 hydroxyalkyl, optionally substituted C 1 -C 6 aminoalkyl, optionally substituted C 2 -C 6 alkenyl, optionally substituted C 2 -C 6 alkynyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl;
R 5 is hydrogen, optionally substituted C 1 -C 6 alkyl, optionally substituted C 1 -C 6 haloalkyl, or optionally substituted C 1 -C 6 deuteroalkyl; R 7 is hydrogen, optionally substituted C 1 -C 6 alkyl, optionally substituted C 1 -C 6 haloalkyl, or optionally substituted C 1 -C 6 deuteroalkyl;
e ach Ra is independently C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl,
C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is
independently optionally substituted with one or more oxo, deuterium, halogen, -CN, -OH, -OMe, - NH 2 , -C(=O)Me, -C(=O)OH, -C(=O)OMe, C 1 -C 6 alkyl, or C 1 -C 6 haloalkyl;
each R b is independently hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is
independently optionally substituted with one or more oxo, deuterium, halogen, -CN, -OH, -OMe, - NH 2 , -C(=O)Me, -C(=O)OH, -C(=O)OMe, C 1 -C 6 alkyl, or C 1 -C 6 haloalkyl; and
each R c and R d is independently hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl,
C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more oxo, deuterium, halogen, -CN, -OH, -OMe, -NH 2 , -C(=O)Me, -C(=O)OH, -C(=O)OMe, C 1 -C 6 alkyl, or C 1 -C 6 haloalkyl;
or R c and R d are taken together with the nitrogen atom to which they are attached to form a
heterocycloalkyl optionally substituted with one or more oxo, deuterium, halogen, -CN, -OH, -OMe, -NH 2 , -C(=O)Me, -C(=O)OH, -C(=O)OMe, C 1 -C 6 alkyl, or C 1 -C 6 haloalkyl.
[00127] Also disclosed herein is a compound of Formula (II), or a pharmaceutically acceptable salt, solvate, or stereoisomer thereof:
wherein:
L is a 4-10 atom linker; optionally substituted with one or more R L ;
each R L is independently deuterium, halogen, -CN, -OR b , -SR b , -S(=O)R a , -S(=O) 2 R a , -NO 2 , -NR c R d , - NHS(=O) 2 R a , -S(=O) 2 NR c R d , -C(=O)R a , -OC(=O)R a , -C(=O)OR b , -OC(=O)OR b , -C(=O)NR c R d , - OC(=O)NR c R d , -NR b C(=O)NR c R d , -NR b C(=O)R a , -NR b C(=O)OR b , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; or two R L on the same carbon are taken together to form an oxo, a cycloalkyl, or heterocycloalkyl; or two R L on different carbons are taken together to form a cycloalkyl or heterocycloalkyl;
Ring A is cycloalkyl, heterocycloalkyl, aryl, or heteroaryl;
each R A is independently deuterium, halogen, -CN, -OR b , -SR b , -S(=O)R a , -S(=O) 2 R a , -NO 2 , -NR c R d , - NHS(=O) 2 R a , -S(=O) 2 NR c R d , -C(=O)R a , -OC(=O)R a , -C(=O)OR b , -OC(=O)OR b , -C(=O)NR c R d , - OC(=O)NR c R d , -NR b C(=O)NR c R d , -NR b C(=O)R a , -NR b C(=O)OR b , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, alkenyl, alkynyl, cycloalkyl,
heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more R A1 ; or two R A on the same carbon are taken together to form an oxo;
each R A1 is independently deuterium, halogen, -CN, -OR b , -SR b , -S(=O)R a , -S(=O) 2 R a , -NO 2 , -NR c R d , - NHS(=O) 2 R a , -S(=O) 2 NR c R d , -C(=O)R a , -OC(=O)R a , -C(=O)OR b , -OC(=O)OR b , -C(=O)NR c R d , - OC(=O)NR c R d , -NR b C(=O)NR c R d , -NR b C(=O)R a , -NR b C(=O)OR b , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; or two R A1 on the same carbon are taken together to form an oxo;
n is 0-4;
is a single bond or a double bond;
X 1 and X 2 are -N- or -C=; provided that one of X 1 or X 2 is -N- and the other is -C=;
Y 8 is CR 8 or N;
Y 6 is CR 6 or N;
Y 3 is CR 3 or N;
Y 9 is CR 9 or N;
R 3 , R 6 , R 8 , and R 9 are independently hydrogen, deuterium, halogen, -CN, -OR b , -SR b , -S(=O)R a , - S(=O) 2 R a , -NO 2 , -NR c R d , -NHS(=O) 2 R a , -S(=O) 2 NR c R d , -C(=O)R a , -OC(=O)R a , -C(=O)OR b , - OC(=O)OR b , -C(=O)NR c R d , -OC(=O)NR c R d , -NR b C(=O)NR c R d , -NR b C(=O)R a , -NR b C(=O)OR b , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, or C 2 -C 6 alkynyl;
R 4 is hydrogen, C 1 -C 6 alkyl, C 1 -C 6 heteroalkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl are optionally substituted with one or more R 4a ;
each R 4a is independently deuterium, halogen, -CN, -OR b , -NR c R d , -C(=O)R a , -C(=O)OR b , -C(=O)NR c R d , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; or two R 4a on the same carbon are taken together to form an oxo;
R 5 is hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, or C 1 -C 6 deuteroalkyl; R 7 is hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, or C 1 -C 6 deuteroalkyl;
each R a is independently C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl,
C1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is
independently optionally substituted with one or more oxo, deuterium, halogen, -CN, -OH, -OMe, - NH 2 , -C(=O)Me, -C(=O)OH, -C(=O)OMe, C 1 -C 6 alkyl, or C 1 -C 6 haloalkyl;
each R b is independently hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is
independently optionally substituted with one or more oxo, deuterium, halogen, -CN, -OH, -OMe, - NH 2 , -C(=O)Me, -C(=O)OH, -C(=O)OMe, C 1 -C 6 alkyl, or C 1 -C 6 haloalkyl; and
each R c and R d is independently hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl,
C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more oxo, deuterium, halogen, -CN, -OH, -OMe, -NH 2 , -C(=O)Me, -C(=O)OH, -C(=O)OMe, C 1 -C 6 alkyl, or C 1 -C 6 haloalkyl;
or R c and R d are taken together with the nitrogen atom to which they are attached to form a
heterocycloalkyl optionally substituted with one or more oxo, deuterium, halogen, -CN, -OH, -OMe, -NH 2 , -C(=O)Me, -C(=O)OH, -C(=O)OMe, C 1 -C 6 alkyl, or C 1 -C 6 haloalkyl.
[00128] Also disclosed herein is a compound of Formula (II), or a pharmaceutically acceptable salt, solvate, or stereoisomer thereof:
wherein:
L is a 4-10 atom linker; optionally substituted with one or more R L ;
each R L is independently deuterium, halogen, -CN, -OR b , -SR b , -S(=O)R a , -S(=O) 2 R a , -NO 2 , -NR c R d , - NHS(=O) 2 R a , -S(=O) 2 NR c R d , -C(=O)R a , -OC(=O)R a , -C(=O)OR b , -OC(=O)OR b , -C(=O)NR c R d , - OC(=O)NR c R d , -NR b C(=O)NR c R d , -NR b C(=O)R a , -NR b C(=O)OR b , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; or two R L on the same carbon are taken together to form an oxo, a cycloalkyl, or heterocycloalkyl; or two R L on adjacent carbons are taken together to form a cycloalkyl or heterocycloalkyl; Ring A is cycloalkyl, heterocycloalkyl, aryl, or heteroaryl;
each R A is independently deuterium, halogen, -CN, -OR b , -SR b , -S(=O)R a , -S(=O) 2 R a , -NO 2 , -NR c R d , - N HS(=O) 2 Ra, -S(=O) 2 NRcRd, -C(=O)Ra, -OC(=O)Ra, -C(=O)ORb, -OC(=O)ORb, -C(=O)NRcRd, - OC(=O)NR c R d , -NR b C(=O)NR c R d , -NR b C(=O)R a , -NR b C(=O)OR b , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, alkenyl, alkynyl, cycloalkyl,
heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more R A1 ; or two R A on the same carbon are taken together to form an oxo;
each R A1 is independently deuterium, halogen, -CN, -OR b , -SR b , -S(=O)R a , -S(=O) 2 R a , -NO 2 , -NR c R d , - NHS(=O) 2 R a , -S(=O) 2 NR c R d , -C(=O)R a , -OC(=O)R a , -C(=O)OR b , -OC(=O)OR b , -C(=O)NR c R d , - OC(=O)NR c R d , -NR b C(=O)NR c R d , -NR b C(=O)R a , -NR b C(=O)OR b , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; or two R A1 on the same carbon are taken together to form an oxo;
n is 0-4;
is a single bond or a double bond;
X 1 and X 2 are -N- or -C=; provided that one of X 1 or X 2 is -N- and the other is -C=;
Y 8 is CR 8 or N;
Y 6 is CR 6 or N;
Y 3 is CR 3 or N;
Y 9 is CR 9 or N;
R 3 , R 6 , R 8 , and R 9 are independently hydrogen, deuterium, halogen, -CN, -OR b , -SR b , -S(=O)R a , - S(=O) 2 R a , -NO 2 , -NR c R d , -NHS(=O) 2 R a , -S(=O) 2 NR c R d , -C(=O)R a , -OC(=O)R a , -C(=O)OR b , - OC(=O)OR b , -C(=O)NR c R d , -OC(=O)NR c R d , -NR b C(=O)NR c R d , -NR b C(=O)R a , -NR b C(=O)OR b , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, or C 2 -C 6 alkynyl;
R 4 is hydrogen, C 1 -C 6 alkyl, C 1 -C 6 heteroalkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl are optionally substituted with one or more R 4a ;
each R 4a is independently deuterium, halogen, -CN, -OR b , -NR c R d , -C(=O)R a , -C(=O)OR b , -C(=O)NR c R d , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; or two R 4a on the same carbon are taken together to form an oxo;
R 5 is hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, or C 1 -C 6 deuteroalkyl;
R 7 is hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, or C 1 -C 6 deuteroalkyl; each R a is independently C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl,
C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more oxo, deuterium, halogen, -CN, -OH, -OMe, - NH 2 , -C(=O)Me, -C(=O)OH, -C(=O)OMe, C 1 -C 6 alkyl, or C 1 -C 6 haloalkyl;
each R b is independently hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is
independently optionally substituted with one or more oxo, deuterium, halogen, -CN, -OH, -OMe, - NH 2 , -C(=O)Me, -C(=O)OH, -C(=O)OMe, C 1 -C 6 alkyl, or C 1 -C 6 haloalkyl; and
each R c and R d is independently hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl,
C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more oxo, deuterium, halogen, -CN, -OH, -OMe, -NH 2 , -C(=O)Me, -C(=O)OH, -C(=O)OMe, C 1 -C 6 alkyl, or C 1 -C 6 haloalkyl;
or R c and R d are taken together with the nitrogen atom to which they are attached to form a
heterocycloalkyl optionally substituted with one or more oxo, deuterium, halogen, -CN, -OH, -OMe, -NH 2 , -C(=O)Me, -C(=O)OH, -C(=O)OMe, C 1 -C 6 alkyl, or C 1 -C 6 haloalkyl.
[00129] In some embodiments of a compound of Formula (II), or a pharmaceutically acceptable salt, stereoisomer, or solvate thereof, the compound is of Formula (IIa):
( )
[00130] In some embodiments of a compound of Formula (II), or a pharmaceutically acceptable salt, stereoisomer, or solvate thereof, the compound is of Formula (IIb):
[00131] In some embodiments of a compound of Formula (II), (IIa), or (IIb), Y 9 is N. In some embodiments of a compound of Formula (II), Y 9 is CR 9 .
[00132] In some embodiments of a compound of Formula (II), (IIa), or (IIb), Y 8 is N. In some embodiments of a compound of Formula (II), Y 8 is CR 8 .
[00133] In some embodiments of a compound of Formula (II), (IIa), or (IIb), or a pharmaceutically acceptable salt, stereoisomer, or solvate thereof, the compound is of Formula (IIc):
[00134] In some embodiments of a compound of Formula (II), (IIa), or (IIb), or a pharmaceutically acceptable salt, stereoisomer, or solvate thereof, the compound is of Formula (IId):
[00135] In some embodiments of a compound of Formula (II) or (IIa)-(IId), Y 6 is CR 6 . In some embodiments of a compound of Formula (II), Y 6 is N.
[00136] In some embodiments of a compound of Formula (II) or (IIa)-(IId), Y 3 is CR 3 . In some embodiments of a compound of Formula (II), Y 3 is N.
[00137] In some embodiments of a compound of Formula (II) or (IIa)-(IId), Ring A is heterocycloalkyl, aryl, or heteroaryl. In some embodiments of a compound of Formula (II) or (IIa)-(IId), Ring A is heterocycloalkyl. In some embodiments of a compound of Formula (II) or (IIa)-(IId), Ring A is aryl or heteroaryl. In some embodiments of a compound of Formula (II) or (IIa)-(IId), Ring A is heteroaryl. In some embodiments of a compound of Formula (II) or (IIa)-(IId), Ring A is aryl. In some embodiments of a compound of Formula (II) or (IIa)-(IId), Ring A is phenyl, pyridyl, pyrimidyl, pyrazinyl, or pyridazinyl. In some embodiments of a compound of Formula (II) or (IIa)-(IId), Ring A is a bicyclic heteroaryl. In some embodiments of a compound of Formula (II) or (IIa)-(IId), Ring A is indole, indazole,
benzimidazole, benzotriazole, benzofuran, benzothiazole, benzoisothiazole, benzoxazole,
benzoisoxazole, or benzothiophene. [00138] In some embodiments of a compound of Formula (II) or (IIa)-(IId), n is 0-3. In some embodiments of a compound of Formula (II) or (IIa)-(IId), n is 0-2. In some embodiments of a compound of Formula (II) or (IIa)-(IId), n is 0 or 1. In some embodiments of a compound of Formula (II) or (IIa)- (IId), n is 0. In some embodiments of a compound of Formula (II) or (IIa)-(IId), n is 1. In some embodiments of a compound of Formula (II) or (IIa)-(IId), n is 2. In some embodiments of a compound of Formula (II) or (IIa)-(IId), n is 3. In some embodiments of a compound of Formula (II) or (IIa)-(IId), n is 4.
[00139] In some embodiments of a compound of Formula (II) or (IIa)-(IId), each R A is independently deuterium, halogen, -CN, -OR b , -SR b , -S(=O)R a , -S(=O) 2 R a , -NO 2 , -NR c R d , -NHS(=O) 2 R a , - S(=O) 2 NR c R d , -C(=O)R a , -OC(=O)R a , -C(=O)OR b , -OC(=O)OR b , -C(=O)NR c R d , -OC(=O)NR c R d , - NR b C(=O)NR c R d , -NR b C(=O)R a , -NR b C(=O)OR b , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more R A1 ; or two R A on the same carbon are taken together to form an oxo.
[00140] In some embodiments of a compound of Formula (II) or (IIa)-(IId), each R A is independently deuterium, halogen, -CN, -OR b , -NR c R d , -C(=O)R a , -C(=O)OR b , -C(=O)NR c R d , C 1 -C 6 alkyl,
C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more R A1 ; or two R A on the same carbon are taken together to form an oxo.
[00141] In some embodiments of a compound of Formula (II) or (IIa)-(IId), each R A is independently deuterium, halogen, -CN, -OR b , -NR c R d , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more R A1 ; or two R A on the same carbon are taken together to form an oxo.
[ 00142] In some embodiments of a compound of Formula (II) or (IIa)-(IId), each R A is independently deuterium, halogen, -CN, -OR b , -NR c R d , -C(=O)R a , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, or C 1 -C 6 deuteroalkyl; or two R A on the same carbon are taken together to form an oxo.
[ 00143] In some embodiments of a compound of Formula (II) or (IIa)-(IId), each R A is independently deuterium, halogen, -CN, -OR b , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, or C 1 -C 6 deuteroalkyl; or two R A on the same carbon are taken together to form an oxo.
[00144] In some embodiments of a compound of Formula (II) or (IIa)-(IId), each R A is independently deuterium, halogen, -OR b , or C 1 -C 6 alkyl; or two R A on the same carbon are taken together to form an oxo.
[00145] In some embodiments of a compound of Formula (II) or (IIa)-(IId), each R A is independently deuterium, halogen, -CN, -OR b , -NR c R d , -C(=O)R a , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, or C 1 -C 6 deuteroalkyl. [00146] In some embodiments of a compound of Formula (II) or (IIa)-(IId), each R A is independently deuterium, halogen, -CN, -OR b , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, or C 1 -C 6 deuteroalkyl.
[00147] In some embodiments of a compound of Formula (II) or (IIa)-(IId), each R A is independently deuterium, halogen, -OR b , or C 1 -C 6 alkyl.
[00148] In some embodiments of a compound of Formula (II) or (IIa)-(IId), each R A1 is independently deuterium, halogen, -CN, -OR b , -SR b , -S(=O)R a , -S(=O) 2 R a , -NO 2 , -NR c R d , -NHS(=O) 2 R a , - S(=O) 2 NR c R d , -C(=O)R a , -OC(=O)R a , -C(=O)OR b , -OC(=O)OR b , -C(=O)NR c R d , -OC(=O)NR c R d , - NR b C(=O)NR c R d , -NR b C(=O)R a , -NR b C(=O)OR b , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; or two R A1 on the same carbon are taken together to form an oxo.
[00149] In some embodiments of a compound of Formula (II) or (IIa)-(IId), each R A1 is independently deuterium, halogen, -CN, -OR b , -NR c R d , -C(=O)R a , -C(=O)OR b , -C(=O)NR c R d , C 1 -C 6 alkyl,
C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; or two R A1 on the same carbon are taken together to form an oxo.
[00150] In some embodiments of a compound of Formula (II) or (IIa)-(IId), each R A1 is independently deuterium, halogen, -CN, -OR b , -NR c R d , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, cycloalkyl, or heterocycloalkyl; or two R A1 on the same carbon are taken together to form an oxo.
[00151] In some embodiments of a compound of Formula (II) or (IIa)-(IId), R 3 , R 6 , R 8 , and R 9 are independently hydrogen, deuterium, halogen, -CN, -OR b , -SR b , -S(=O)R a , -S(=O) 2 R a , -NO 2 , -NR c R d , - NHS(=O) 2 R a , -S(=O) 2 NR c R d , -C(=O)R a , -OC(=O)R a , -C(=O)OR b , -OC(=O)OR b , -C(=O)NR c R d , - OC(=O)NR c R d , -NR b C(=O)NR c R d , -NR b C(=O)R a , -NR b C(=O)OR b , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 -C 6 alkenyl, or C 2 -C 6 alkynyl. In some embodiments of a compound of Formula (II) or (IIa)-(IId), R 3 , R 6 , R 8 , and R 9 are independently hydrogen, deuterium, halogen, -CN, -OR b , -NR c R d , -C(=O)NR c R d , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, or C 1 -C 6 deuteroalkyl. In some embodiments of a compound of Formula (II) or (IIa)-(IId), R 3 , R 6 , R 8 , and R 9 are independently hydrogen, deuterium, halogen, or C 1 -C 6 alkyl. In some embodiments of a compound of Formula (II) or (IIa)-(IId), R 3 , R 6 , R 8 , and R 9 are hydrogen.
[00152] In some embodiments of a compound of Formula (II) or (IIa)-(IId), R 4 is hydrogen, C 1 -C 6 alkyl, C 1 -C 6 heteroalkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 2 - C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl are optionally substituted with one or more R 4a .
[00153] In some embodiments of a compound of Formula (II) or (IIa)-(IId), R 4 is hydrogen, C 1 -C 6 alkyl, C 1 -C 6 heteroalkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein each alkyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl are optionally substituted with one or more R 4a . [00154] In some embodiments of a compound of Formula (II) or (IIa)-(IId), R 4 is hydrogen, C 1 -C 6 alkyl, C 1 -C 6 heteroalkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, cycloalkyl, or heterocycloalkyl; wherein each alkyl, cycloalkyl, or heterocycloalkyl are optionally substituted with one or more R 4a .
[00155] In some embodiments of a compound of Formula (II) or (IIa)-(IId), R 4 is hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, or C 1 -C 6 deuteroalkyl.
[00156] In some embodiments of a compound of Formula (II) or (IIa)-(IId), R 4 is hydrogen, C 1 -C 6 alkyl, or C 1 -C 6 deuteroalkyl.
[00157] In some embodiments of a compound of Formula (II) or (IIa)-(IId), R 4 is hydrogen or
C 1 -C 6 alkyl optionally substituted with one or more R 4a . In some embodiments of a compound of Formula (II) or (IIa)-(IId), R 4 is C 1 -C 6 alkyl optionally substituted with one or more R 4a .
[00158] In some embodiments of a compound of Formula (II) or (IIa)-(IId), R 4 is hydrogen or
C 1 -C 6 alkyl. In some embodiments of a compound of Formula (II) or (IIa)-(IId), R 4 is C 1 -C 6 alkyl.
[00159] In some embodiments of a compound of Formula (II) or (IIa)-(IId), each R 4a is independently deuterium, halogen, -CN, -OR b , -NR c R d , -C(=O)R a , -C(=O)OR b , -C(=O)NR c R d , C 1 -C 6 alkyl,
C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; or two R 4a on the same carbon are taken together to form an oxo.
[00160] In some embodiments of a compound of Formula (II) or (IIa)-(IId), each R 4a is independently deuterium, halogen, -CN, -OR b , -NR c R d , -C(=O)R a , -C(=O)OR b , -C(=O)NR c R d , C 1 -C 6 alkyl,
C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, cycloalkyl, or heterocycloalkyl; or two R 4a on the same carbon are taken together to form an oxo.
[ 00161] In some embodiments of a compound of Formula (II) or (IIa)-(IId), each R 4a is independently deuterium, halogen, -CN, -OR b , -NR c R d , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, cycloalkyl, or heterocycloalkyl; or two R 4a on the same carbon are taken together to form an oxo.
[00162] In some embodiments of a compound of Formula (II) or (IIa)-(IId), R 5 is hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, or C 1 -C 6 deuteroalkyl. In some embodiments of a compound of Formula (II) or (IIa)-(IId), R 5 is hydrogen or C 1 -C 6 alkyl. In some embodiments of a compound of Formula (II) or (IIa)-(IId), R 5 is hydrogen.
[00163] In some embodiments of a compound of Formula (II) or (IIa)-(IId), R 7 is hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, or C 1 -C 6 deuteroalkyl. In some embodiments of a compound of Formula (II) or (IIa)-(IId), R 7 is hydrogen or C 1 -C 6 alkyl. In some embodiments of a compound of Formula (II) or (IIa)-(IId), R 7 is hydrogen.
[00164] In some embodiments of a compound of Formula (II) or (IIa)-(IId), L is an a C2-10 alkylene chain optionally substituted with one or more R L , wherein up to four carbon atoms of L are optionally and independently replaced by -NR L -, -S-, -O-, -OC(=O)-, -C(=O)O-, -C(=O)-, -C(=O)NR L -, - NR L C(=O)-, -S(=O) 2 NR L -, -NR L S(=O) 2 -, -NR L C(=O)NR L -, -S(O)-, or -S(O)2-. [00165] In some embodiments of a compound of Formula (II) or (IIa)-(IId), L is an a C 2-10 alkylene chain optionally substituted with one or more R L , wherein up to four carbon atoms of L are optionally and independently replaced by -NR L -, -S-, -O-, -C(=O)-, -S(O)-, or -S(O) 2 -.
[00166] In some embodiments of a compound of Formula (II) or (IIa)-(IId), L is an a C 2-10 alkylene chain optionally substituted with one or more R L , wherein up to four carbon atoms of L are optionally and independently replaced by -NR L -, -O-, or -C(=O)-.
[00167] In some embodiments of a compound of Formula (II) or (IIa)-(IId), L is a 4-10 atom linker; optionally substituted with one or more R L .
[00168] In some embodiments of a compound of Formula (II) or (IIa)-(IId), L is a 4-8 atom linker; optionally substituted with one or more R L .
[00169] In some embodiments of a compound of Formula (II) or (IIa)-(IId), L is a 4-6 atom linker; optionally substituted with one or more R L .
[00170] In some embodiments of a compound of Formula (II) or (IIa)-(IId), L is a 4-10 atom linker comprising between 4 and 10 carbons and between 0 and 4 heteroatoms selected from oxygen and nitrogen; the linker being optionally substituted with one or more R L .
[00171] In some embodiments of a compound of Formula (II) or (IIa)-(IId), L is a 4-10 atom linker comprising between 3 and 9 carbons and between 1 and 2 heteroatoms selected from oxygen and nitrogen; the linker being optionally substituted with one or more R L .
[00172] In some embodiments of a compound of Formula (II) or (IIa)-(IId), L is a 4-8 atom linker comprising between 4 and 8 carbons and between 0 and 4 heteroatoms selected from oxygen and nitrogen; the linker being optionally substituted with one or more R L .
[00173] In some embodiments of a compound of Formula (II) or (IIa)-(IId), L is a 4-8 atom linker comprising between 3 and 7 carbons and between 1 and 2 heteroatoms selected from oxygen and nitrogen; the linker being optionally substituted with one or more R L .
[00174] In some embodiments of a compound of Formula (II) or (IIa)-(IId), L is a 4-6 atom linker comprising between 4 and 6 carbons and between 0 and 4 heteroatoms selected from oxygen and nitrogen; the linker being optionally substituted with one or more R L .
[00175] In some embodiments of a compound of Formula (II) or (IIa)-(IId), L is a 4-6 atom linker comprising between 3 and 5 carbons and between 1 and 2 heteroatoms selected from oxygen and nitrogen; the linker being optionally substituted with one or more R L .
[00176] In some embodiments of a compound of Formula (II) or (IIa)-(IId), each R L is independently deuterium, halogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, or C 1 -C 6 deuteroalkyl; or two R L on the same carbon are taken together to form an oxo or a cycloalkyl; or two R L on different carbons are taken together to form a cycloalkyl.
[00177] In some embodiments of a compound of Formula (II) or (IIa)-(IId), each R L is independently deuterium, halogen, -CN, -OR b , -NR c R d , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, or C 1 -C 6 deuteroalkyl; or two R L on the same carbon are taken together to form an oxo. [00178] In some embodiments of a compound of Formula (II) or (IIa)-(IId), each R L is independently deuterium, halogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, or C 1 -C 6 deuteroalkyl; or two R L on the same carbon are taken together to form an oxo.
[00179] In some embodiments of a compound of Formula (II) or (IIa)-(IId), each R L is independently deuterium, halogen, or C 1 -C 6 alkyl; or two R L on the same carbon are taken together to form an oxo.
[00180] In some embodiments of a compound of Formula (II) or (IIa)-(IId), each R L is independently deuterium or halogen; or two R L on the same carbon are taken together to form an oxo. [00181] In some embodiments of a compound of Formula (II) or (IIa)-(IId), L is or ; wherein Z 1 and Z 2 are independently -O-, -S-, or -NR Z ; each R Z is
independently hydrogen or C 1 -C 6 alkyl; and L 1 and L 2 are independently C 1 -C 6 alkylene optionally substituted with one or more R L . [00182] In some embodiments, is equivalent to . In some embodiments, is equivalent to .
[00183] In some embodiments of a compound of Formula (II) or (IIa)-(IId), Z 1 and Z 2 are
independently -O- or -NR Z ; each R Z is independently hydrogen or C 1 -C 6 alkyl. In some embodiments of a compound of Formula (II) or (IIa)-(IId), Z 1 and Z 2 are independently -O- or -NR Z ; each R Z is hydrogen.
[00184] In some embodiments of a compound of Formula (II) or (IIa)-(IId), L 1 and L 2 are
independently C 1 -C 3 alkylene optionally substituted with one or more R L .
In some embodiments of a compound of Formula (II) or (IIa)-(IId), L is
[00185] In some embodiments of a compound of Formula (II) or (IIa)-(IId), L is ,
[00186] In some embodiments of a compound of Formula (II) or (IIa)-(IId), L is or
.
[00187] In some embodiments of a compound described above, each R a is independently C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, or cycloalkyl; wherein each alkyl and cycloalkyl is independently optionally substituted with one or more oxo, deuterium, halogen, -CN, -OH, -OMe, -NH 2 , -C(=O)Me, - C(=O)OH, -C(=O)OMe, C 1 -C 6 alkyl, or C 1 -C 6 haloalkyl. In some embodiments of a compound described above, each R a is independently C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, or C 1 -C 6 deuteroalkyl. In some embodiments of a compound described above, each R a is independently C 1 -C 6 alkyl.
[00188] In some embodiments of a compound described above, each R b is independently hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, or cycloalkyl; wherein each alkyl and cycloalkyl is independently optionally substituted with one or more oxo, deuterium, halogen, -CN, -OH, -OMe, -NH 2 , -C(=O)Me, -C(=O)OH, -C(=O)OMe, C 1 -C 6 alkyl, or C 1 -C 6 haloalkyl. In some embodiments of a compound described above, each R b is independently hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, or
C 1 -C 6 deuteroalkyl. In some embodiments of a compound described above, each R b is independently hydrogen or C 1 -C 6 alkyl. In some embodiments of a compound described above, each R b is hydrogen. In some embodiments of a compound described above, each R b is independently C 1 -C 6 alkyl.
[00189] In some embodiments of a compound described above, each R c and R d is independently hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 deuteroalkyl, or cycloalkyl; wherein each alkyl and cycloalkyl is independently optionally substituted with one or more oxo, deuterium, halogen, -CN, -OH, - OMe, -NH 2 , -C(=O)Me, -C(=O)OH, -C(=O)OMe, C 1 -C 6 alkyl, or C 1 -C 6 haloalkyl. In some embodiments of a compound described above, each R c and R d is independently hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, or C 1 -C 6 deuteroalkyl. In some embodiments of a compound described above, each R c and R d is independently hydrogen or C 1 -C 6 alkyl. In some embodiments of a compound described above, each R c and R d is hydrogen. In some embodiments of a compound described above, each R c and R d is independently C 1 -C 6 alkyl.
[00190] In some embodiments of a compound of Formula (II) or (IIa)-(IId), each L, R L , R A , R 4 , R a , R b , R c , and R d is independently substituted with one, two, three, or four substituents as defined herein. In some embodiments of a compound of Formula (II) or (IIa)-(IId), each L, R L , R A , R 4 , R a , R b , R c , and R d is independently optionally substituted with one, two, or three substituents as defined herein. In some embodiments of a compound of Formula (II) or (IIa)-(IId), each L, R L , R A , R 4 , R a , R b , R c , and R d is independently optionally substituted with one or two substituents as defined herein. In some embodiments of a compound of Formula (II) or (IIa)-(IId), each L, R L , R A , R 4 , R a , R b , R c , and R d is independently optionally substituted with one substituent as defined herein.
[00191] In some embodiments of a compound of Formula (II) or (IIa)-(IId), the compound is: [00192] In some embodiments of a compound of Formula (II) or (IIa)-(IId), the compound is:
[00193] In some embodiments of a compound of Formula (II) or (IIa)-(IId), the compound is:
Further Forms of Compounds Disclosed Herein
Isomers/Stereoisomers
[00194] In some embodiments, the compounds described herein exist as geometric isomers. In some embodiments, the compounds described herein possess one or more double bonds. The compounds presented herein include all cis, trans, syn, anti, entgegen (E), and zusammen (Z) isomers as well as the corresponding mixtures thereof. In some situations, the compounds described herein possess one or more chiral centers and each center exists in the R configuration or S configuration. The compounds described herein include all diastereomeric, enantiomeric, and epimeric forms as well as the corresponding mixtures thereof. In additional embodiments of the compounds and methods provided herein, mixtures of enantiomers and/or diastereoisomers, resulting from a single preparative step, combination, or interconversion are useful for the applications described herein. In some embodiments, the compounds described herein are prepared as their individual stereoisomers by reacting a racemic mixture of the compound with an optically active resolving agent to form a pair of diastereoisomeric compounds, separating the diastereomers, and recovering the optically pure enantiomers. In some embodiments, dissociable complexes are preferred. In some embodiments, the diastereomers have distinct physical properties (e.g., melting points, boiling points, solubilities, reactivity, etc.) and are separated by taking advantage of these dissimilarities. In some embodiments, the diastereomers are separated by chiral chromatography, or preferably, by separation/resolution techniques based upon differences in solubility. In some embodiments, the optically pure enantiomer is then recovered, along with the resolving agent. Labeled compounds
[00195] In some embodiments, the compounds described herein exist in their isotopically-labeled forms. In some embodiments, the methods disclosed herein include methods of treating diseases by administering such isotopically-labeled compounds. In some embodiments, the methods disclosed herein include methods of treating diseases by administering such isotopically-labeled compounds as pharmaceutical compositions. Thus, in some embodiments, the compounds disclosed herein include isotopically-labeled compounds, which are identical to those recited herein, but for the fact that one or more atoms are replaced by an atom having an atomic mass or mass number different from the atomic mass or mass number usually found in nature. Examples of isotopes that can be incorporated into compounds described herein, or a solvate, or stereoisomer thereof, include isotopes of hydrogen, carbon, nitrogen, oxygen, phosphorous, sulfur, fluorine, and chloride, such as 2H, 3H, 13C, 14C, l5N, 18O, 17O, 31P, 32 P, 35 S, 18 F, and 36 Cl, respectively. Compounds described herein, and the pharmaceutically acceptable salts, solvates, or stereoisomers thereof which contain the aforementioned isotopes and/or other isotopes of other atoms are within the scope of this disclosure. Certain isotopically-labeled compounds, for example those into which radioactive isotopes such as 3 H and 14 C are incorporated, are useful in drug and/or substrate tissue distribution assays. Tritiated, i.e., 3 H and carbon-14, i.e., 14 C, isotopes are particularly preferred for their ease of preparation and detectability. Further, substitution with heavy isotopes such as deuterium, i.e., 2 H, produces certain therapeutic advantages resulting from greater metabolic stability, for example increased in vivo half-life or reduced dosage requirements. In some embodiments, the isotopically labeled compound or a pharmaceutically acceptable salt, solvate, or stereoisomer thereof is prepared by any suitable method.
[00196] In some embodiments, the compounds described herein are labeled by other means, including, but not limited to, the use of chromophores or fluorescent moieties, bioluminescent labels, or chemiluminescent labels.
Pharmaceutically acceptable salts
[00197] In some embodiments, the compounds described herein exist as their pharmaceutically acceptable salts. In some embodiments, the methods disclosed herein include methods of treating diseases by administering such pharmaceutically acceptable salts. In some embodiments, the methods disclosed herein include methods of treating diseases by administering such pharmaceutically acceptable salts as pharmaceutical compositions.
[00198] In some embodiments, the compounds described herein possess acidic or basic groups and therefor react with any of a number of inorganic or organic bases, and inorganic and organic acids, to form a pharmaceutically acceptable salt. In some embodiments, these salts are prepared in situ during the final isolation and purification of the compounds disclosed herein, or by separately reacting a purified compound in its free form with a suitable acid or base, and isolating the salt thus formed.
[00199] Examples of pharmaceutically acceptable salts include those salts prepared by reaction of the compounds described herein with a mineral, organic acid, or inorganic base, such salts including acetate, acrylate, adipate, alginate, aspartate, benzoate, benzenesulfonate, bisulfate, bisulfite, bromide, butyrate, butyn-1,4-dioate, camphorate, camphorsulfonate, caproate, caprylate, chlorobenzoate, chloride, citrate, cyclopentanepropionate, decanoate, digluconate, dihydrogenphosphate, dinitrobenzoate, dodecylsulfate, ethanesulfonate, formate, fumarate, glucoheptanoate, glycerophosphate, glycolate, hemisulfate, heptanoate, hexanoate, hexyne-1,6-dioate, hydroxybenzoate, g-hydroxybutyrate, hydrochloride, hydrobromide, hydroiodide, 2-hydroxyethanesulfonate, iodide, isobutyrate, lactate, maleate, malonate, methanesulfonate, mandelate metaphosphate, methanesulfonate, methoxybenzoate, methylbenzoate, monohydrogenphosphate, 1-napthalenesulfonate, 2-napthalenesulfonate, nicotinate, nitrate, palmoate, pectinate, persulfate, 3-phenylpropionate, phosphate, picrate, pivalate, propionate, pyrosulfate, pyrophosphate, propiolate, phthalate, phenylacetate, phenylbutyrate, propanesulfonate, salicylate, succinate, sulfate, sulfite, succinate, suberate, sebacate, sulfonate, tartrate, thiocyanate,
tosylateundeconate, and xylenesulfonate.
[00200] Further, the compounds described herein can be prepared as pharmaceutically acceptable salts formed by reacting the free base form of the compound with a pharmaceutically acceptable inorganic or organic acid, including, but not limited to, inorganic acids such as hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid metaphosphoric acid, and the like; and organic acids such as acetic acid, propionic acid, hexanoic acid, cyclopentanepropionic acid, glycolic acid, pyruvic acid, lactic acid, malonic acid, succinic acid, malic acid, maleic acid, fumaric acid, p-toluenesulfonic acid, tartaric acid, trifluoroacetic acid, citric acid, benzoic acid, 3-(4-hydroxybenzoyl)benzoic acid, cinnamic acid, mandelic acid, arylsulfonic acid, methanesulfonic acid, ethanesulfonic acid, 1,2-ethanedisulfonic acid, 2- hydroxyethanesulfonic acid, benzenesulfonic acid, 2-naphthalenesulfonic acid, 4-methylbicyclo- [2.2.2]oct-2-ene-1-carboxylic acid, glucoheptonic acid, 4,4’-methylenebis-(3-hydroxy-2-ene-1- carboxylic acid), 3-phenylpropionic acid, trimethylacetic acid, tertiary butylacetic acid, lauryl sulfuric acid, gluconic acid, glutamic acid, hydroxynaphthoic acid, salicylic acid, stearic acid, and muconic acid.
[00201] In some embodiments, those compounds described herein which comprise a free acid group react with a suitable base, such as the hydroxide, carbonate, bicarbonate, or sulfate of a pharmaceutically acceptable metal cation, with ammonia, or with a pharmaceutically acceptable organic primary, secondary, tertiary, or quaternary amine. Representative salts include the alkali or alkaline earth salts, like lithium, sodium, potassium, calcium, and magnesium, and aluminum salts and the like. Illustrative examples of bases include sodium hydroxide, potassium hydroxide, choline hydroxide, sodium carbonate, N + (C 1-4 alkyl) 4 , and the like.
[00202] Representative organic amines useful for the formation of base addition salts include ethylamine, diethylamine, ethylenediamine, ethanolamine, diethanolamine, piperazine, and the like. It should be understood that the compounds described herein also include the quaternization of any basic nitrogen-containing groups they contain. In some embodiments, water or oil-soluble or dispersible products are obtained by such quaternization.
Solvates
[00203] In some embodiments, the compounds described herein exist as solvates. The disclosure provides for methods of treating diseases by administering such solvates. The disclosure further provides for methods of treating diseases by administering such solvates as pharmaceutical compositions.
[00204] Solvates contain either stoichiometric or non-stoichiometric amounts of a solvent, and, in some embodiments, are formed during the process of crystallization with pharmaceutically acceptable solvents such as water, ethanol, and the like. Hydrates are formed when the solvent is water, or alcoholates are formed when the solvent is alcohol. Solvates of the compounds described herein can be conveniently prepared or formed during the processes described herein. In addition, the compounds provided herein can exist in unsolvated as well as solvated forms. In general, the solvated forms are considered equivalent to the unsolvated forms for the purposes of the compounds and methods provided herein.
Tautomers
[00205] In some situations, compounds exist as tautomers. The compounds described herein include all possible tautomers within the formulas described herein. Tautomers are compounds that are
interconvertible by migration of a hydrogen atom, accompanied by a switch of a single bond and adjacent double bond. In bonding arrangements where tautomerization is possible, a chemical equilibrium of the tautomers will exist. All tautomeric forms of the compounds disclosed herein are contemplated. The exact ratio of the tautomers depends on several factors, including temperature, solvent, and pH. Preparation of the Compounds
[00206] The compounds used in the reactions described herein are made according to organic synthesis techniques known to those skilled in this art, starting from commercially available chemicals and/or from compounds described in the chemical literature.“Commercially available chemicals” are obtained from standard commercial sources including Acros Organics (Pittsburgh, PA), Aldrich Chemical (Milwaukee, WI, including Sigma Chemical and Fluka), Apin Chemicals Ltd. (Milton Park, UK), Avocado Research (Lancashire, U.K.), BDH, Inc. (Toronto, Canada), Bionet (Cornwall, U.K.), Chem Service Inc. (West Chester, PA), Crescent Chemical Co. (Hauppauge, NY), Eastman Organic Chemicals, Eastman Kodak Company (Rochester, NY), Fisher Scientific Co. (Pittsburgh, PA), Fisons Chemicals (Leicestershire, UK), Frontier Scientific (Logan, UT), ICN Biomedicals, Inc. (Costa Mesa, CA), Key Organics (Cornwall, U.K.), Lancaster Synthesis (Windham, NH), Maybridge Chemical Co. Ltd. (Cornwall, U.K.), Parish Chemical Co. (Orem, UT), Pfaltz & Bauer, Inc. (Waterbury, CN), Polyorganix (Houston, TX), Pierce Chemical Co. (Rockford, IL), Riedel de Haen AG (Hanover, Germany), Spectrum Quality Product, Inc. (New Brunswick, NJ), TCI America (Portland, OR), Trans World Chemicals, Inc. (Rockville, MD), and Wako Chemicals USA, Inc. (Richmond, VA).
[00207] Suitable reference books and treatises that detail the synthesis of reactants useful in the preparation of compounds described herein, or provide references to articles that describe the preparation, include for example,“Synthetic Organic Chemistry”, John Wiley & Sons, Inc., New York; S. R. Sandler et al.,“Organic Functional Group Preparations,” 2nd Ed., Academic Press, New York, 1983; H. O. House, “Modern Synthetic Reactions”, 2nd Ed., W. A. Benjamin, Inc. Menlo Park, Calif.1972; T. L. Gilchrist, “Heterocyclic Chemistry”, 2nd Ed., John Wiley & Sons, New York, 1992; J. March,“Advanced Organic Chemistry: Reactions, Mechanisms and Structure”, 4th Ed., Wiley-Interscience, New York, 1992. Additional suitable reference books and treatises that detail the synthesis of reactants useful in the preparation of compounds described herein, or provide references to articles that describe the preparation, include for example, Fuhrhop, J. and Penzlin G.“Organic Synthesis: Concepts, Methods, Starting Materials”, Second, Revised and Enlarged Edition (1994) John Wiley & Sons ISBN: 3-527-29074-5; Hoffman, R.V. “Organic Chemistry, An Intermediate Text” (1996) Oxford University Press, ISBN 0-19-509618-5; Larock, R. C.“Comprehensive Organic Transformations: A Guide to Functional Group Preparations” 2nd Edition (1999) Wiley-VCH, ISBN: 0-471-19031-4; March, J.“Advanced Organic Chemistry:
Reactions, Mechanisms, and Structure” 4th Edition (1992) John Wiley & Sons, ISBN: 0-471-60180-2; Otera, J. (editor)“Modern Carbonyl Chemistry” (2000) Wiley-VCH, ISBN: 3-527-29871-1; Patai, S. “Patai's 1992 Guide to the Chemistry of Functional Groups” (1992) Interscience ISBN: 0-471-93022-9; Solomons, T. W. G.“Organic Chemistry” 7th Edition (2000) John Wiley & Sons, ISBN: 0-471-19095-0; Stowell, J.C.,“Intermediate Organic Chemistry” 2nd Edition (1993) Wiley-Interscience, ISBN: 0-471- 57456-2;“Industrial Organic Chemicals: Starting Materials and Intermediates: An Ullmann's
Encyclopedia” (1999) John Wiley & Sons, ISBN: 3-527-29645-X, in 8 volumes;“Organic Reactions” (1942-2000) John Wiley & Sons, in over 55 volumes; and“Chemistry of Functional Groups” John Wiley & Sons, in 73 volumes.
[00208] Specific and analogous reactants are optionally identified through the indices of known chemicals prepared by the Chemical Abstract Service of the American Chemical Society, which are available in most public and university libraries, as well as through on-line. Chemicals that are known but not commercially available in catalogs are optionally prepared by custom chemical synthesis houses, where many of the standard chemical supply houses (e.g., those listed above) provide custom synthesis services. A reference for the preparation and selection of pharmaceutical salts of the compounds described herein is P. H. Stahl & C. G. Wermuth“Handbook of Pharmaceutical Salts”, Verlag Helvetica Chimica Acta, Zurich, 2002. Pharmaceutical Compositions
[00209] In certain embodiments, the compound described herein is administered as a pure chemical. In some embodiments, the compound described herein is combined with a pharmaceutically suitable or acceptable carrier (also referred to herein as a pharmaceutically suitable (or acceptable) excipient, physiologically suitable (or acceptable) excipient, or physiologically suitable (or acceptable) carrier) selected on the basis of a chosen route of administration and standard pharmaceutical practice as described, for example, in Remington: The Science and Practice of Pharmacy (Gennaro, 21 st Ed. Mack Pub. Co., Easton, PA (2005)).
[00210] Accordingly, provided herein is a pharmaceutical composition comprising a compound described herein, or a pharmaceutically acceptable salt, solvate, or stereoisomer thereof, and a pharmaceutically acceptable excipient.
[00211] In certain embodiments, the compound provided herein is substantially pure, in that it contains less than about 5%, or less than about 1%, or less than about 0.1%, of other organic small molecules, such as unreacted intermediates or synthesis by-products that are created, for example, in one or more of the steps of a synthesis method.
[00212] Pharmaceutical compositions are administered in a manner appropriate to the disease to be treated (or prevented). An appropriate dose and a suitable duration and frequency of administration will be determined by such factors as the condition of the patient, the type and severity of the patient's disease, the particular form of the active ingredient, and the method of administration. In general, an appropriate dose and treatment regimen provides the composition(s) in an amount sufficient to provide therapeutic and/or prophylactic benefit (e.g., an improved clinical outcome, such as more frequent complete or partial remissions, or longer disease-free and/or overall survival, or a lessening of symptom severity. Optimal doses are generally determined using experimental models and/or clinical trials. The optimal dose depends upon the body mass, weight, or blood volume of the patient.
[00213] In some embodiments, the pharmaceutical composition is formulated for oral, topical (including buccal and sublingual), rectal, vaginal, transdermal, parenteral, intrapulmonary, intradermal, intrathecal and epidural and intranasal administration. Parenteral administration includes intramuscular, intravenous, intraarterial, intraperitoneal, or subcutaneous administration. In some embodiments, the pharmaceutical composition is formulated for intravenous injection, oral administration, inhalation, nasal administration, topical administration, or ophthalmic administration. In some embodiments, the pharmaceutical composition is formulated for oral administration. In some embodiments, the pharmaceutical composition is formulated for intravenous injection. In some embodiments, the pharmaceutical composition is formulated as a tablet, a pill, a capsule, a liquid, an inhalant, a nasal spray solution, a suppository, a suspension, a gel, a colloid, a dispersion, a suspension, a solution, an emulsion, an ointment, a lotion, an eye drop, or an ear drop. In some embodiments, the pharmaceutical composition is formulated as a tablet.
[00214] Suitable doses and dosage regimens are determined by conventional range-finding techniques known to those of ordinary skill in the art. Generally, treatment is initiated with smaller dosages that are less than the optimum dose of the compound disclosed herein. Thereafter, the dosage is increased by small increments until the optimum effect under the circumstances is reached. In some embodiments, the present method involve the administration of about 0.1 µg to about 50 mg of at least one compound described herein per kg body weight of the subject. For a 70 kg patient, dosages of from about 10 µg to about 200 mg of the compound disclosed herein would be more commonly used, depending on a subject’s physiological response.
[00215] By way of example only, the dose of the compound described herein for methods of treating a disease as described herein is about 0.001 to about 1 mg/kg body weight of the subject per day, for example, about 0.001 mg, about 0.002 mg, about 0.005 mg, about 0.010 mg, 0.015 mg, about 0.020 mg, about 0.025 mg, about 0.050 mg, about 0.075 mg, about 0.1 mg, about 0.15 mg, about 0.2 mg, about 0.25 mg, about 0.5 mg, about 0.75 mg, or about 1 mg/kg body weight per day. In some embodiments, the dose of compound described herein for the described methods is about 1 to about 1000 mg/kg body weight of the subject being treated per day, for example, about 1 mg, about 2 mg, about 5 mg, about 10 mg, about 15 mg, about 20 mg, about 25 mg, about 50 mg, about 75 mg, about 100 mg, about 150 mg, about 200 mg, about 250 mg, about 500 mg, about 750 mg, or about 1000 mg per day. Methods of Treatment [00216] The compounds disclosed herein, or pharmaceutically acceptable salts, solvates, or stereoisomers thereof, are useful for the inhibition of kinase activity of one or more enzymes. In some embodiments the kinase inhibited by the compounds and methods is TYK2.
[00217] Provided herein are compounds that are inhibitors of TYK2 and are therefore useful for treating one or more disorders associated with activity of TYK2 or mutants thereof.
[00218] Provided herein are methods for treating a disease or disorder, wherein the disease or disorder is an autoimmune disorders, inflammatory disorders, proliferative disorders, endocrine disorders, neurological disorders, or disorders associated with transplantation, said method comprising
administering to a patient in need thereof, a pharmaceutical composition comprising an effective amount of a compound described herein, or a pharmaceutically acceptable salt, solvate, or stereoisomer thereof.
[00219] In some embodiments, the disease or disorder is an autoimmune disorder. In some
embodiments the disease or disorder is selected from type 1 diabetes, systemic lupus erythematosus, multiple sclerosis, psoriasis, Behçet's disease, POEMS syndrome, Crohn's disease, ulcerative colitis, and inflammatory bowel disease.
[00220] In some embodiments, the disease or disorder is an inflammatory disorder. In some embodiments, the inflammatory disorder is rheumatoid arthritis, asthma, chronic obstructive pulmonary disease, psoriasis, hepatomegaly, Crohn's disease, ulcerative colitis, inflammatory bowel disease.
[00221] In some embodiments, the disease or disorder is a proliferative disorder. In some embodiments, the proliferative disorder is a hematological cancer. In some embodiments the proliferative disorder is a leukemia. In some embodiments, the leukemia is a T-cell leukemia. In some embodiments the T-cell leukemia is T-cell acute lymphoblastic leukemia (T-ALL). In some embodiments the proliferative disorder is polycythemia vera, myelofibrosis, essential or thrombocytosis.
[00222] In some embodiments, the disease or disorder is an endocrine disorder. In some embodiments, the endocrine disorder is polycystic ovary syndrome, Crouzon's syndrome, or type 1 diabetes.
[00223] In some embodiments, the disease or disorder is a neurological disorder. In some
embodiments, the neurological disorder is Alzheimer's disease.
[00224] In some embodiments the proliferative disorder is associated with one or more activating mutations in TYK2. In some embodiments, the activating mutation in TYK2 is a mutation to the FERM domain, the JH2 domain, or the kinase domain. In some embodiments the activating mutation in TYK2 is selected from G36D, S47N, R425H, V731I, E957D, and R1027H.
[00225] In some embodiments, the disease or disorder is associated with transplantation. In some embodiments the disease or disorder associated with transplantation is transplant rejection, or graft versus host disease.
[00226] In some embodiments the disease or disorder is associated with type I interferon, IL-10, IL-12, or IL-23 signaling. In some embodiments the disease or disorder is associated with type I interferon signaling. In some embodiments the disease or disorder is associated with IL-10 signaling. In some embodiments the disorder is associated with IL-12 signaling. In some embodiments the disease or disorder is associated with IL-23 signaling.
[00227] Provided herein are methods for treating an inflammatory or allergic condition of the skin, for example psoriasis, contact dermatitis, atopic dermatitis, alopecia areata, erythema multiforma, dermatitis herpetiformis, scleroderma, vitiligo, hypersensitivity angiitis, urticaria, bullous pemphigoid, lupus erythematosus, systemic lupus erythematosus, pemphigus vulgaris, pemphigus foliaceus, paraneoplastic pemphigus, epidermolysis bullosa acquisita, acne vulgaris, and other inflammatory or allergic conditions of the skin.
[00228] Provided herein are methods for treating other diseases or conditions, such as diseases or conditions having an inflammatory component, for example, treatment of diseases and conditions of the eye such as ocular allergy, conjunctivitis, keratoconjunctivitis sicca, and vernal conjunctivitis, diseases affecting the nose including allergic rhinitis, and inflammatory disease in which autoimmune reactions are implicated or having an autoimmune component or etiology, including autoimmune hematological disorders (e.g. hemolytic anemia, aplastic anemia, pure red cell anemia and idiopathic
thrombocytopenia), systemic lupus erythematosus, rheumatoid arthritis, polychondritis, scleroderma, Wegener granulamatosis, dermatomyositis, chronic active hepatitis, myasthenia gravis, Steven-Johnson syndrome, idiopathic sprue, autoimmune inflammatory bowel disease (e.g. ulcerative colitis and Crohn's disease), irritable bowel syndrome, celiac disease, periodontitis, hyaline membrane disease, kidney disease, glomerular disease, alcoholic liver disease, multiple sclerosis, endocrine opthalmopathy, Grave's disease, sarcoidosis, alveolitis, chronic hypersensitivity pneumonitis, multiple sclerosis, primary biliary cirrhosis, uveitis (anterior and posterior), Sjogren's syndrome, keratoconjunctivitis sicca and vernal keratoconjunctivitis, interstitial lung fibrosis, psoriatic arthritis, systemic juvenile idiopathic arthritis, cryopyrin-associated periodic syndrome, nephritis, vasculitis, diverticulitis, interstitial cystitis, glomerulonephritis (with and without nephrotic syndrome, e.g. including idiopathic nephrotic syndrome or minal change nephropathy), chronic granulomatous disease, endometriosis, leptospiriosis renal disease, glaucoma, retinal disease, ageing, headache, pain, complex regional pain syndrome, cardiac hypertrophy, musclewasting, catabolic disorders, obesity, fetal growth retardation, hyperchlolesterolemia, heart disease, chronic heart failure, mesothelioma, anhidrotic ecodermal dysplasia, Behcet's disease, incontinentia pigmenti, Paget's disease, pancreatitis, hereditary periodic fever syndrome, asthma (allergic and non-allergic, mild, moderate, severe, bronchitic, and exercise-induced), acute lung injury, acute respiratory distress syndrome, eosinophilia, hypersensitivities, anaphylaxis, nasal sinusitis, ocular allergy, silica induced diseases, COPD (reduction of damage, airways inflammation, bronchial hyperreactivity, remodeling or disease progression), pulmonary disease, cystic fibrosis, acid-induced lung injury, pulmonary hypertension, polyneuropathy, cataracts, muscle inflammation in conjunction with systemic sclerosis, inclusion body myositis, myasthenia gravis, thyroiditis, Addison's disease, lichen planus, Type 1 diabetes, or Type 2 diabetes, appendicitis, atopic dermatitis, asthma, allergy, blepharitis, bronchiolitis, bronchitis, bursitis, cervicitis, cholangitis, cholecystitis, chronic graft rejection, colitis, conjunctivitis, Crohn's disease, cystitis, dacryoadenitis, dermatitis, dermatomyositis, encephalitis, endocarditis, endometritis, enteritis, enterocolitis, epicondylitis, epididymitis, fasciitis, fibrositis, gastritis, gastroenteritis, Henoch-Schonlein purpura, hepatitis, hidradenitis suppurativa, immunoglobulin A nephropathy, interstitial lung disease, laryngitis, mastitis, meningitis, myelitis myocarditis, myositis, nephritis, oophoritis, orchitis, osteitis, otitis, pancreatitis, parotitis, pericarditis, peritonitis, pharyngitis, pleuritis, phlebitis, pneumonitis, pneumonia, polymyositis, proctitis, prostatitis, pyelonephritis, rhinitis, salpingitis, sinusitis, stomatitis, synovitis, tendonitis, tonsillitis, ulcerative colitis, uveitis, vaginitis, vasculitis, or vulvitis.
[00229] In some embodiments the inflammatory disease is acute and chronic gout, chronic gouty arthritis, psoriasis, psoriatic arthritis, rheumatoid arthritis, Juvenile rheumatoid arthritis, Systemic juvenile idiopathic arthritis (SJIA), Cryopyrin Associated Periodic Syndrome (CAPS), or osteoarthritis.
[00230] In some embodiments the inflammatory disease is a Th1 or Th17 mediated disease. In some embodiments the Th17 mediated disease is selected from Systemic lupus erythematosus, Multiple sclerosis, and inflammatory bowel disease (including Crohn's disease or ulcerative colitis).
[00231] In some embodiments the inflammatory disease is Sjogren's syndrome, allergic disorders, osteoarthritis, conditions of the eye such as ocular allergy, conjunctivitis, keratoconjunctivitis sicca, vernal conjunctivitis, or diseases affecting the nose such as allergic rhinitis. Combination Therapy
[00232] In certain instances, the compound described herein, or a pharmaceutically acceptable salt, solvate, or stereoisomer thereof, is administered in combination with a second therapeutic agent.
[00233] In some embodiments, the benefit experienced by a patient is increased by administering one of the compounds described herein with a second therapeutic agent (which also includes a therapeutic regimen) that also has therapeutic benefit.
[00234] In one specific embodiment, a compound described herein, or a pharmaceutically acceptable salt, solvate, or stereoisomer thereof, is co-administered with a second therapeutic agent, wherein the compound described herein, or a pharmaceutically acceptable salt, solvate, or stereoisomer thereof, and the second therapeutic agent modulate different aspects of the disease, disorder or condition being treated, thereby providing a greater overall benefit than administration of either therapeutic agent alone.
[00235] In any case, regardless of the disease, disorder or condition being treated, the overall benefit experienced by the patient is simply additive of the two therapeutic agents or the patient experiences a synergistic benefit.
[00236] In certain embodiments, different therapeutically-effective dosages of the compounds disclosed herein will be utilized in formulating a pharmaceutical composition and/or in treatment regimens when the compounds disclosed herein are administered in combination with a second therapeutic agent.
Therapeutically-effective dosages of drugs and other agents for use in combination treatment regimens are optionally determined by means similar to those set forth hereinabove for the actives themselves. Furthermore, the methods of prevention/treatment described herein encompasses the use of metronomic dosing, i.e., providing more frequent, lower doses in order to minimize toxic side effects. In some embodiments, a combination treatment regimen encompasses treatment regimens in which administration of a compound described herein, or a pharmaceutically acceptable salt, solvate, or stereoisomer thereof, is initiated prior to, during, or after treatment with a second agent described herein, and continues until any time during treatment with the second agent or after termination of treatment with the second agent. It also includes treatments in which a compound described herein, or a pharmaceutically acceptable salt, solvate, or stereoisomer thereof, and the second agent being used in combination are administered simultaneously or at different times and/or at decreasing or increasing intervals during the treatment period. Combination treatment further includes periodic treatments that start and stop at various times to assist with the clinical management of the patient.
[00237] It is understood that the dosage regimen to treat, prevent, or ameliorate the condition(s) for which relief is sought, is modified in accordance with a variety of factors (e.g. the disease, disorder or condition from which the subject suffers; the age, weight, sex, diet, and medical condition of the subject). Thus, in some instances, the dosage regimen actually employed varies and, in some embodiments, deviates from the dosage regimens set forth herein.
[00238] For combination therapies described herein, dosages of the co-administered compounds vary depending on the type of co-drug employed, on the specific drug employed, on the disease or condition being treated, and so forth. In additional embodiments, when co-administered with a second therapeutic agent, the compound provided herein is administered either simultaneously with the second therapeutic agent, or sequentially.
[00239] In combination therapies, the multiple therapeutic agents (one of which is one of the compounds described herein) are administered in any order or even simultaneously. If administration is simultaneous, the multiple therapeutic agents are, by way of example only, provided in a single, unified form, or in multiple forms (e.g., as a single pill or as two separate pills).
[00240] The compounds described herein, or a pharmaceutically acceptable salt, solvate, or stereoisomer thereof, as well as combination therapies, are administered before, during, or after the occurrence of a disease or condition, and the timing of administering the composition containing a compound varies. Thus, in one embodiment, the compounds described herein are used as a prophylactic and are administered continuously to subjects with a propensity to develop conditions or diseases in order to prevent the occurrence of the disease or condition. In another embodiment, the compounds and compositions are administered to a subject during or as soon as possible after the onset of the symptoms. In specific embodiments, a compound described herein is administered as soon as is practicable after the onset of a disease or condition is detected or suspected, and for a length of time necessary for the treatment of the disease. In some embodiments, the length required for treatment varies, and the treatment length is adjusted to suit the specific needs of each subject. For example, in specific embodiments, a compound described herein or a formulation containing the compound is administered for at least 2 weeks, about 1 month to about 5 years.
[00241] In some embodiments, the compound of described herein, or a pharmaceutically acceptable salt, solvate, or stereoisomer thereof, is administered in combination with an adjuvant. In one embodiment, the therapeutic effectiveness of one of the compounds described herein is enhanced by administration of an adjuvant (i.e., by itself the adjuvant has minimal therapeutic benefit, but in combination with another therapeutic agent, the overall therapeutic benefit to the patient is enhanced). EXAMPLES
Example 1: General Procedure for synthesis of compound Example 1
Step 1: Example 1b
[00242] To a solution of Example 1a (11.0 g, 63.74 mmol) in MeOH (200 mL) was added NaOMe (6.88 g, 127.48 mmol). The reaction mixture was stirred at 70°C for 6 h. The mixture was quenched with H 2 O (1 L), and a pink precipitate was formed. The solid was collected by filtration and dried in vacuum to afford Example 1b (9.0 g, 84.0% yield) as a pink solid. LCMS [M+1] + = 169.1.
Step 2: Example 1c [00243] To a mixture of Example 1b (5.0 g, 29.76 mmol) in CCl 4 (100 mL) were added NBS (6.36 g, 35.71 mmol) and AIBN (0.98 g, 5.95 mmol). The reaction mixture was stirred at 80°C for 16 h under N 2 protection. After cooled to room temperature, the solvent was removed, and the residue was purified by silica gel flash column chromatography afford the product Example 1c (2.8 g, 38.2% yield) as a yellow solid.
LCMS [M+1] + = 247.1.
Step 3: Example 1d
[00244] To a solution of tert-butyl (2-hydroxyethyl)carbamate (1.29 g, 8.02 mmol) in THF (30 mL) was added NaH (320mg, 8.02 mmol) in portions at 0 °C. The mixture was stirred for 30 min at the same temperature, then Example 1c (1.8 g, 7.29 mmol) in THF was added dropwise. The reaction mixture was stirred at r.t. for 4 h. The solvent was removed, and the residue was purified by silica gel flash column chromatography to afford the product Example 1d (600 mg, 25.2% yield) as yellow oil. LCMS [M+1- 100] + = 328.2.
Step 4: Example 1e
[00245] To a solution of Example 1d (600 mg, 3.3 mmol) in DCM (5 mL) was added TFA(1 mL). The reaction mixture was stirred at r.t. for 2 h. The solution was concentrated in vacuum to give the crude product Example 1e (710 mg, 113.8%, crude) as yellow oil, which was used to next step directly without purification. LCMS [M+1] + = 228.2.
Step 5: Example 1h
[00246] To a solution of Example 1f (2.0 g, 7.69 mmol) in EtOH (40 mL) were added CH3NH 2 (7.7 mL, 2M in MeOH, 15.38 mmol) and K 2 CO 3 (2.12 g, 15.38mmol). The reaction mixture was stirred at r.t. for 4 h. The mixture was poured into H 2 O (200 mL), and a white precipitate was formed. The solid was collected by filtration and dried in vacuo to afford the product Example 1h (1.85 g, 94.4% yield) as a white solid. LCMS [M+1] + = 255.2.
Step 6: Example 1i
[ 00247] To a solution of Example 1h (1.85 g, 7.26 mmol) in dioxane (40 mL) were added Boc 2 O (1.9 g, 8.72 mmol), TEA (1.09 g, 10.89 mmol) and DMAP (44 mg, 0.36 mmol). The reaction mixture was stirred at r.t. for 4 h. The solvent was removed, and the residue was purified by silica gel flash column chromatography to afford the product Example 1i (2.3 g, 89.3% yield) as an off-white solid. LCMS [M+1] + = 355.2.
Step 7: Example 1j
[00248] To a mixture of Example 1i (800 mg, 2.25 mmol) in toluene (10 mL) was added TBTO (2.96 g, 4.51 mmol). The reaction mixture was stirred at 120°C for 24 h under N 2 protection. After cooled to room temperature, the solvent was removed, and the residue was purified by silica gel flash column chromatography to afford the product Example 1j (620 mg, 84.3% yield) as a yellow solid. LCMS
[M+1] + = 327.2.
Step 8: Example 1l [00249] To a solution of Example 1j (500 mg, 1.53 mmol) in DCM (10 mL) was added SOCl 2 (728 mg, 6.12 mmol), and the reaction mixture was stirred at r.t. for 1 h. The solvent was removed, and the residue was diluted with DCM, which was added to a solution of Example 1e (565 mg, 1.53 mmol) and TEA (773 mg, 7.65 mmol) in DCM (10 mL) dropwise at 0°C. The resulting mixture was stirred at r.t. for 1 h, and then concentrated. The residue was purified by silica gel flash column chromatography to afford the product Example 1l (180 mg, 22.0% yield) as an off-white solid. LCMS [M+1] + = 536.3.
Step 9: Example 1m
[00250] To a mixture of Example 1l (160 mg, 0.299 mmol) in MeOH (30 mL) was added Pd/C (16 mg) under N 2 protection. The suspension was degassed under vacuum and purged with H 2 , which was stirred at r.t. for 30 min under H 2 balloon. The solid was filtered out, and the filtrate was concentrated. The residue was purified by silica gel flash column chromatography to afford the product Example 1m (82 mg, 54.2% yield) as a yellow solid. LCMS[M+1] + = 506.2.
Step 10: Example 1n
[00251] To a solution of Example 1m (80 mg, 0.16 mmol) in 1,4-dioxane (5 mL) were added Cs 2 CO 3 (103 mg, 0.32 mmol) and 3rd-t-Bu-Xphos-Pd (14 mg, 0.016 mmol). The reaction mixture was stirred at 90°C for 16 h under N 2 . After cooled to room temperature, the solvent was removed, and the residue was purified by silica gel flash column chromatography to afford the product Example 1n (50 mg, 67.4% yield) as an off-white solid. LCMS [M+1] + = 470.2.
Step 11: Example 1
[00252] To a solution of Example 1n (50 mg, 0.11 mmol) in DCM (2 mL) was added TFA (0.5 mL). The reaction mixture was stirred at r.t. for 2 h and then concentrated in vacuum. The residue was purified by prep-HPLC to give the desired product Example 51 (17.0 mg, 43.2% yield) as a white solid.
LCMS[M+1] + = 370.2. 1 H NMR (300 MHz, DMSO-d6) d 9.14 (s, 1H), 8.57 (d, 1H), 8.14 (s, 1H), 8.06 (brs, 1H), 7.93 (d, 1H), 7.75 (d, 1H), 5.95 (s, 1H), 4.53 (s, 2H), 3.97 (s, 3H), 3.59 (t, 2H), 3.36 - 3.32 (m, 2H), 2.92 (d, 3H).
Example 2: General Procedure for synthesis of compound Example 2
Step 1: Example 2b
[00253] To a solution of Example 2a (10.0 g, 59.8 mmol, 1.0 eq) in CCl 4 (200 mL) were added NBS (10.8 g, 60.4 mmol, 1.01 eq) and AIBN (1.96 g,12.0 mmol, 0.20 eq). The reaction mixture was stirred at 80°C for 18 h under N2. After cooled to room temperature, the solvent was removed, and the residue was purified by silica gel flash column chromatography to afford the product Example 2b (7.2 g, 49% yield) as a yellow solid.
LCMS [M+1] + = 245.2.
Step 2: Example 2d
[00254] To a solution of Example 2b (1.0 g, 4.06 mmol, 1.0 eq) and Example 2c (720 mg, 4.47 mmol, 1.1 eq) in DMF (20 mL) was added NaH (244 mg, 60% in mineral oil, 6.1 mmol, 1.5 eq) in portions at 0°C. The reaction mixture was stirred for 4 h at r.t., and then poured into a saturated aqueous of NH 4 Cl (40 mL), which was extracted with EtOAc (50 mL*3). The combined organic layer was washed with brine, dried over Na 2 SO 4 , and concentrated. The crude product was purified by silica gel flash column chromatography to afford the product Example 2d (1.2 g, 90% yield) as yellow oil. LCMS [M+1] + = 327.2.
Step 3: Example 2e
[00255] To a solution of Example 2d (800 mg, 2.5 mmol, 1.0 eq) in DCM (8 mL) was added
HCl/dioxane (1 mL, 4M in dioxane). The reaction solution was stirred for 2 h at r.t. After completion, the reaction mixture was concentrated to afford the product Example 2e (660 mg, 83% yield) as a yellow solid. LCMS [M+1] + = 227.2
Step 4: Example 2g
[00256] To a solution of Example 2f (1.0 g, 2.8 mmol, 1.0 eq, from Example 1i) in toluene (15 mL) was added TBTO (3.3 g, 5.6 mmol, 2.0 eq). The reaction mixture was stirred at reflux for 24 h under N 2 . After concentrated, the residue was purified by silica gel flash column chromatography to afford the product Example 2g (800 mg, 82% yield) as a yellow solid. LCMS [M+1] + = 327.2.
Step 5: Example 2h
[00257] To a solution of Example 2g (452 mg, 1.7 mmol, 0.8 eq) in DCM (10 mL) were added SOCl 2 (1.04 g, 8.8 mmol, 4.0 eq) and DMF (0.2 mL), the reaction mixture was stirred for 0.5 h at r.t. After the reaction completed, it was concentrated in vacuo to give crude product, which was diluted with DCM and then added to a solution of Example 2e (700 mg, 2.2 mmol, 1.0 eq) and TEA (1.1 g, 11.0 mmol, 5.0 eq) in DCM (10 mL) dropwise at 0 o C. The resulting mixture was stirred for 0.5 h at r.t. The solvent was removed, and the residue was purified by silica gel flash column chromatography to afford the product Example 2h (300 mg, 3% yield) as a yellow solid. LCMS [M+1] + = 535.3.
Step 6: Example 2i
[00258] To a solution of Example 2h (170 mg, 0.30 mmol, 1.0 eq) in MeOH (30 mL) was added Pd/C (17 mg). The suspension was degassed under vacuum and purged with H 2 , which was stirred for 0.5 h at r.t. under H 2 balloon. The solid was filtered out, and the filtrate was concentrated. The residue was purified by silica gel flash column chromatography to afford the product Example 2i (107 mg, 71% yield) as yellow oil.
LCMS [M+1] + =505.2.
Step 7: Example 2j
[00259] Toa solution of Example 2i (100 mg, 0.20 mmol, 1.0 eq) in dioxane (5 mL) were added Cs 2 CO 3 (130.4 mg, 0.40 mmol, 2.0 eq) and 3 rd t-Bu-Xphos-Pd (17.8 mg, 0.02 mmol, 0.1 eq). The reaction mixture was stirred for 2 h at 80 o C under N2 protection. The solid was filtered out and filtrate was concentrated, and the residue was purified by Prep-TLC to afford the Example 2j (50 mg, 53% yield) as a yellow solid.
LCMS [M+1] + =469.3.
Step 8: Example 2
[00260] To a solution of Example 2j (50 mg,0.10 mmol, 1.0 eq) in DCM (2 mL) was added HCl/dioxane (1mL, 4M in THF) dropwise at 0 o C. The reaction mixture was stirred for 2 h at r.t. After completion, the reaction mixture was concentrated and the residue was purified by Prep-HPLC to give the desired product Example 2 (17.0 mg, 46% yield) as a light yellow solid. LCMS [M+1] + = 369.2. 1 H NMR (300 MHz, DMSO-d6) d 8.91 (s, 1H), 8.32 (d, 1H), 8.22 (brs, 1H), 8.12 (s, 1H), 7.82 (q, 1H), 7.00 (d, 1H), 6.92 (dd, 1H), 5.95 (s, 1H), 4.50 (s, 2H), 3.88 (s, 3H), 3.57 (t, 2H),3.44-3.32 (m, 2H), 2.92 (d, 3H).
Example 3: General Procedure for synthesis of compound Example 3
Step 1: Example 3c
[00261] To a solution of Example 3a (2.0 g, 11.69 mmol, 1.0 eq) and Example 3b (2.45 g, 14.03 mmol,1.2 eq) in dry THF (20 mL) were added PPh 3 (3.69 g, 14.03 mmol,1.2 eq) and DBAD (3.22 g, 14.03 mmol,1.2 eq) at 0 °C under N 2 , which was stirred for 2 h at r.t. The solvent was removed under vacuum, and the residue was purified by silica gel flash column chromatography to give the desired product Example 3c (2.5 g, 64.9% yield) as a white solid. LCMS [M+1] + = 327.3
Step 2: Example 3d
[00262] To a solution of Example 3c (1.0 g, 3.06 mmol, 1.0 eq) in MeOH (10 mL) was added 10% Pd/C (100 mg) under N 2 protection. The suspension was degassed under vacuum and purged with H 2 three times. The mixture was stirred at r.t. for 1 h under H 2 balloon. The suspension was filtered through a Celite pad and the filter cake was washed with MeOH. The combined filtrates were concentrated in vacuo to give the desired product Example 3d (900 mg, 99.1% yield) as colorless oil. LCMS [M+1] + = 297.3
Step 3: Example 3f
[00263] To a mixture of Example 3d (100 mg, 0.28 mmol, 1.0 eq), Example 3e (108.7 mg, 0.40 mmol, 1.5 eq, from Example 1i) and Cs 2 CO 3 (183.6 mg, 0.56 mmol, 2.0 eq) in dioxane (5 mL) were added Pd(OAc)2 (6.4 mg, 0.028 mmol, 0.1 eq), BINAP (35.1 mg, 0.056 mmol, 0.2 eq). The mixture was degassed with N2 three times, and stirred for 18 h at 90°C. The reaction was concentrated in vacuo. The residue was purified by silica gel flash column chromatography to give the desired product Example 3f (130 mg, 75.9% yield) as a light brown solid. LCMS [M+1] + = 615.4
Step 4: Example 3g
[00264] To a solution of Example 3f (130 mg, 1.78 mmol, 1.0 eq) in EtOH (30 mL) and H 2 O (10 mL) was added NaOH (12.7 mg, 1.5mmol, 1.0 eq) at 0 °C. The mixture was stirred for 16 h at 80°C. The solvent was removed to afford the crude product Example 3g (160 mg, quant.) as a white solid. LCMS [M+1] + = 587.4
Step 5: Example 3h
[00265] To a solution of Example 3g (160 mg, 0.27 mmol,1.0 eq) in MeOH (2 mL) was added HCl/dioxane (1.0 mL, 4M in dioxane), which was stirred at r.t. for 2 h. The mixture was concentrated, and the residue was treated with EtOAc (30 mL) to give the crude product Example 3h (150 mg, quant.) as a white solid. LCMS [M+1] + = 387.4.
Step 6: Example 3
[00266] To a solution of Example 3h (crude, 135 mg, 0.30 mmol, 1.0 eq), DIEA (196.7 mg, 1.52 mmol, 5.0 eq) in DMF (10 mL) was added HATU (138.6 mg, 0.37 mmol, 1.2 eq). The mixture was stirred at r.t. for 1 h. EtOAc (40 mL)was added to the reaction mixture, which was washed with brine (20 mL*2), dried over Na 2 SO 4 and concentrated. The residue was purified by Prep-HPLC to afford the desired product Example 3 (3.3 mg, 3.1% yield) as a white solid. LCMS [M+1] + = 369.1. 1 H NMR (300 MHz, DMSO-d 6 ) d 8.87 (s, 1H), 8.44 (s, 1H), 8.25-8.11 (m, 2H), 7.88 (s, 1H), 6.98 (s, 1H), 6.51 (s, 1H), 6.07 (s, 1H), 4.32-4.21 (m, 2H), 3.84 (s, 3H), 3.22-3.15 (m, 2H), 2.92 (s, 3H), 2.02-1.81(m, 2H).
Example 4: General Procedure for synthesis of compound Example 4
Step 1: Example 4c
[00267] To a solution of Example 4a (1 g, 5.92 mmol) in THF (10 mL) were added Example 4b (1.04 g, 5.92 mmol) and PPh 3 (1.86 g, 7.1 mmol). The mixture was cooled to 0 o C and DIAD (1.4 g, 7.1 mmol) was added dropwise. The resulting mixture was stirred at room temperature for 1 h under N 2 . The reaction mixture was extracted with EtOAc (50 mL*2). The combined organic phase was washed with brine, dried over Na 2 SO 4 , filtrated and the filtrate was concentrated under reduced pressure. The residue was purified by silica gel chromatography to give Example 4c (3 g, crude) as yellow oil.
L CMS [M+1-100] + = 227.1
Step 2: Example 4d
[00268] To a solution of Example 4c (crude, 3 g, 9.2 mmol) in DCM (20 mL) was added TFA (10 mL). The mixture was stirred at room temperature for 2 h. The reaction mixture was concentrated. The residue was extracted with DCM (50 mL*2) and H 2 O. The aqueous layer was alkalization with NaHCO 3 and extracted with DCM (50 mL*2). The combined organic phase was washed with brine, dried over Na 2 SO 4 , filtrated and the filtrate was concentrated under reduced pressure to give Example 4d (700 mg, yield 34%) as yellow oil. LCMS [M+1] + = 227.1. 1 H NMR (400 MHz, Chloroform-d) d 7.39 (d, 1H), 7.10 (dd, 1H), 7.01 (d, 1H), 4.04 (t, 2H), 3.90 (s, 3H), 2.91 (t, 2H), 1.92 (p, 2H).
Step 3: Example 4f
[00269] To a solution of Example 4e (10 g, 77.5 mmol) in MeOH (100 mL) was added NaHCO 3 (13 g, 155.0 mmol) at 0 o C. Then Br2 (18.6 g, 116.3 mmol) was added dropwise and the resulting mixture was stirred at room temperature overnight. One half of the volume of solvent was removed under reduced pressure. The remaining was poured into ice water. The solid formed was collected and dried to give Example 4f (14.5 g, yield 90%) as a red solid. LCMS [M+1] + = 209.9. 1 H NMR (400 MHz, DMSO-d6) d7.98 (s, 1H), 6.96 (s, 2H).
Step 4: Example 4h
[00270] To a solution of Example 4f (14.5 g, 69.7 mmol) in EtOH (100 mL) was added Example 4g (16.7 g, 111.5 mmol). The mixture was stirred at 80 o C overnight under N2. The reaction mixture was concentrated. The residue was purified by silica gel chromatography to give Example 4h (7 g, yield 39%) (Brominated 36% & Chlorinated 64%) as a yellow solid. LCMS [M+1] + = 260.0/306.0. 1 H NMR (400 MHz, Chloroform-d) d 8.37 (d, 1H), 7.57 (s, 0.36 H), 7.38 (s, 0.64H), 4.46 (q, 2H), 1.43 (t, 3H). Step 5: Example 4j
[00271] To a solution of Example 4h (640 mg, 2.46 mmol) in dioxane (6 mL) were added Example 4i (409 mg, 2.71 mmol) and TEA (497 mg, 4.92 mmol). The mixture was stirred at 90 o C for 2 h under N2. The reaction mixture was concentrated to provide a sludge that was triturated with H 2 O (5 mL) to provide a solid which was filtered, rinsed with H 2 O and then collected with DCM (20 mL). The solution was dried over Na 2 SO 4 , filtrated and the filtrate was concentrated under reduced pressure to give Example 4j (880 mg, yield 82%) as a yellow solid. LCMS [M+1] + = 375.1. 1 H NMR (400 MHz, Chloroform-d) d 8.10 (s, 1H), 7.15 (d, 2H), 6.85 (d, 2H), 6.10 (s, 1H), 5.48 (s, 2H), 4.43 (q, 2H), 3.78 (s, 3H), 3.16 (s, 3H), 1.41 (t, 3H).
Step 6: Example 4k
[00272] To a solution of Example 4j (680 mg, 1.81 mmol) in THF/MeOH/H 2 O (9 mL/9 mL/6 mL) was added LiOH.H 2 O (305 mg, 7.25 mmol). The mixture was stirred at room temperature overnight. The THF/MeOH were removed in vacuum, the resulting solution was adjusted to pH = 4 using 1M HCl. The mixture was extracted with DCM (30 mL*2). The combined organic phase was washed with brine, dried over Na 2 SO 4 , filtrated and the filtrate was concentrated under reduced pressure to give Example 4k (760 mg, yield 93%) as a yellow solid. LCMS [M+1] + = 347.1.
Step 7: Example 4l
[00273] To a solution of Example 4k (660 mg, 1.9 mmol) in DMF (6 mL) were added Example 4d (430 mg, 1.9 mmol), TEA (576 mg, 5.7 mmol) and HATU (867 mg, 2.28 mmol). The mixture was stirred at room temperature for 2 h under N 2 . The reaction mixture was extracted with EtOAc (30 mL*2). The combined organic phase was washed with brine, dried over Na 2 SO 4 , filtrated and the filtrate was concentrated under reduced pressure. The residue was purified by silica gel chromatography to give Example 4l (960 mg, yield 87%) as a yellow solid. LCMS [M+1] + = 555.2.
Step 8: Example 4m
[00274] To a solution of Example 4l (30 mg, 0.054 mmol) in THF/HOAc (0.5 mL/0.05 mL) was added Zn (35 mg, 0.54 mmol). The mixture was stirred at room temperature overnight. The reaction mixture was alkalization with a.q NaHCO 3 and extracted with DCM (10 mL*2). The combined organic phase was washed with brine, dried over Na 2 SO 4 , filtrated and the filtrate was concentrated under reduced pressure to give Example 4m (30 mg, crude) as yellow oil. LCMS [M+1] + = 525.2.
Step 9: Example 4n
[00275] To a solution of Example 4m (30 mg, 0.06mmol) in dioxane (2 mL) were added Cs 2 CO 3 (37 mg, 0.11 mmol) and 3 rd -tBu-Xphos-Pd (5 mg, 0.006 mmol). The mixture was stirred at 90 o C for 2 h under N2. The reaction mixture was extracted with DCM (10 mL*2). The combined organic phase was washed with brine, dried over Na 2 SO 4 , filtrated and the filtrate was concentrated under reduced pressure to give Example 4n (40 mg, crude) as yellow oil. LCMS [M+1] + = 489.2.
Step 10: Example 4
[00276] To a solution of Example 4n (crude, 0.06 mmol) in DCM (1 mL) was added HCl/EtOAc (0.3 mL). The mixture was stirred at room temperature for 2 h. The mixture was concentrated under reduced pressure. The residue was purified by prep-HPLC to give Example 4 (5.1 mg, yield 23%) as a yellow solid. LCMS [M+1] + = 369.1. 1 H NMR (400 MHz, Chloroform-d) d 8.82 (s, 1H), 8.10-8.07 (m, 2H), 7.00 (s, 1H), 6.87 (d, 1H), 6.65 (s, 1H), 6.57 (dd, 1H), 5.66 (s, 1H), 4.33 (t, 2H), 3.91 (s, 3H), 3.50-3.44 (m, 2H), 3.06 (d, 3H), 2.12-2.03 (m, 2H).
Example 5:
Step 1: Example 5b
[00277] To a solution of Example 5a (10.0 g, 56.8 mmol, 1.0 eq) in MeOH (50 mL) was added NaOMe (4.6 g, 85.2 mmol, 1.5 eq) at 0 °C. The reaction mixture was stirred at 50 °C for 2 h. The mixture was concentrated in vacuo. The residue was purified by silica gel flash column chromatography to afford the desired product Example 5a (1.5 g, yield 14.1%) as a yellow solid. LCMS [M+1] + = 189.1.
Step 2: Example 5c
[00278] The solution of Example 5b (1.5g, 13.58 mmol, 1.0 eq) in HBr/AcOH (20 mL) was stirred at 100°C for 16 h. The reaction mixture was concentrated in vacuo. The residue was diluted with H 2 O (20 mL) and basified with saturated NaHCO 3 aqueous solution to pH ~8. The aqueous was extracted with EtOAc (50 mL*3). The combined organic layer was washed with brine, dried over Na 2 SO 4 and concentrated in vacuo. The residue was purified by silica gel flash column chromatography to afford the desired product Example 5c (1.0 g, yield 57.5%) as a yellow solid. LCMS [M+1] + = 219.1.
Step 3: Example 5e [00279] To a solution of Example 5c (900 mg, 4.13 mmol, 1.0 eq) and Example 5d (867 mg, 4.95 mmol,1.2 eq) in dry DCM (20 mL) was added PPh 3 (1.3 g, 4.95 mmol,1.2 eq), followed by DBAD (1.13 g, 4.95 mmol,1.2 eq) at 0 °C under N 2 . The reaction mixture was stirred for 3 h at r.t. The solvent was removed under vacuum, and the residue was purified by silica gel flash column chromatography to give the desired product Example 5e (950 mg, yield 61.4%) as a yellow solid. LCMS [M+1] + = 376.2.
Step 4: Example 5f
[00280] To a solution of Example 5e (950 mg, 2.54 mmol, 1.0 eq) in MeOH (20 mL) was added NaOMe (412 mg, 7.62 mmol, 3.0 eq). The reaction mixture was stirred at 65°C for 2 h. The mixture was concentrated in vacuo. The residue was purified by silica gel flash column chromatography to afford the product Example 5f (700 mg, yield 84.5%) as a yellow solid. LCMS [M+1] + = 328.3.
Step 5: Example 5g
[00281] To a solution of Example 5f (650 mg, 1.98 mmol, 1.0 eq) in MeOH (20 mL) was added 10% Pd/C (60 mg) under N2 protection. The mixture was degassed with H 2 three times and stirred at r.t. for 1 h under H 2 balloon. The solid was filtered. The filtrate was concentrated in vacuo to give the desired product Example 5g (550 mg, yield 93.2%) as colorless oil. LCMS [M+1] + = 298.3.
Step 6: Example 5i
[00282] To a mixture of Example 5f (5.0 g, 38.75 mmol, 1.0 eq) and NaHCO 3 (9.76 g, 116.2 mmol, 3.0 eq) in MeOH (30 mL) was added Br2 (7.4 g, 46.51 mmol, 1.2 eq) dropwise at 0°C. After addition, it was warmed to r.t. and stirred for 16 h. The reaction mixture concentrated in vacuo. The residue was purified by silica gel flash column chromatography to afford the desired product Example 5i (3.5 g, yield 43.6%) as a yellow solid. LCMS [M+1] + = 208.1.
Step 7: Example 5k
[00283] To a solution of Example 5i (3.5 g, 16.9 mmol, 1.0 eq) in EtOH (50 mL) was added Example 5j (5.07 g, 33.8 mmol, 2.0 eq), which was stirred at 80°C for 16 h. The reaction mixture concentrated in vacuo. The residue was purified by silica gel flash column chromatography to give the desired product Example 5k (1.2 g, yield 34.2%) as a white solid. LCMS [M+1] + = 260.1.
Step 8: Example 5l
[00284] To a mixture of Example 5k (1.2 g, 4.61mmol, 1.0 eq) and K 2 CO 3 (1.08 g, 13.8 mmol, 3.0 eq) in THF (20 mL) was added methanamine hydrochloride (467 mg, 6.91 mmol, 1.5 eq), which was stirred at r.t. for 2 h, The reaction mixture concentrated in vacuo. The residue was purified by silica gel flash column chromatography to give the desired product Example 5l (1.05 g, yield 87.5%) as a yellow solid. LCMS [M+1] + = 255.2.
Step 9: Example 5m
[00285] To a solution of Example 5l (1.05 g, 3.92 mmol, 1.0 eq), Et 3 N(1.19 g, 11.76 mmol, 3.0 eq) and DMAP (47.5 mg, 0.39 mmol, 0.1 eq) in DCM (15 mL) was added Boc 2 O (1.27 g, 5.88 mmol, 1.5 eq) at 0°C, which was stirred for 1 h at room temperature. The reaction mixture concentrated in vacuo. The residue was purified by silica gel flash column chromatography to give the desired product Example 5m (1.1 g, yield 75.3%) as a white solid. LCMS [M+1] + = 355.2.
Step 10: Example 5n
[00286] To a mixture of Example 5m (350 mg, 0.99 mmol, 1.0 eq), Example 5g (352 mg, 1.18 mmol, 1.2 eq) and Cs 2 CO 3 (643 mg, 20.0 mmol, 2.0 eq) in dioxane (10 mL) were added Pd(OAc) 2 (22 mg, 0.099 mmol, 0.1 eq) and BINAP (134 mg, 0.198 mmol, 0.2 eq). The mixture was degassed with N 2 three times, and then heated to 90°C for 16 h. The reaction was concentrated in vacuo. The residue was purified by silica gel flash column chromatography to give the desired product Example 5n (290 mg, yield 47.7%) as a light brown solid. LCMS [M+1] + = 616.4.
Step 11: Example 5o
[00287] To a solution of Example 5n (280 mg, 0.46 mmol, 1.0 eq) in EtOH (2.5 mL) and H 2 O (0.8 mL) was added NaOH (36.5 mg, 0.91 mmol, 2.0 eq) at 0°C. The mixture was heated to 80°C and stirred at for16 h. The reaction mixture was concentrated in vacuo to afford the crude product Example 5o (360 mg, crude, quant.) as a white solid. LCMS [M+1] + = 588.4.
Step 12: Example 5p
[00288] To a solution of Example 5o (350 mg, 0.596 mmol, 1.0 eq) in DCM (2 mL) was added HCl/dioxane (1.0 mL, 4 M in dioxane), which was stirred at r.t. for 1 h. The mixture was concentrated in vacuo and treated with EtOAc (30 mL) to give the crude product Example 5p (160 mg, yield 58.4%) as a white solid. LCMS [M+1] + = 388.4.
Step 13: Example 5
[00289] To a solution of Example 5p (160 mg, 0.35 mmol, 1.0 eq) and DIEA (135 mg, 1.04 mmol, 3.0 eq) in DMF (5 mL) was added HATU (199 mg, 0.52 mmol, 1.5 eq). The mixture was stirred at r.t. for 2 h. EtOAc (10 mL) was added to the reaction mixture and washed with brine (10 mL*2). The organic layer was concentrated in vacuo. The residue was purified by Prep-HPLC to afford the desired product Example 5 (4.3 mg, yield 3.3%) as a white solid. LCMS [M+1] + = 370.2. 1 H NMR (300 MHz, DMSO- d6) d 8.71 (s, 1H), 8.63 (d, 1H), 8.60-8.57 (m, 1H), 7.83 (s, 1H), 7.58-7.55 (m, 1H), 7.43 (d, 1H), 6.33 (s, 1H), 4.33-4.29 (m, 2H), 3.94 (s, 3H), 3.27-3.26 (m, 2H), 2.88 (d, 3H), 1.95-1.86 (m, 2H).
Example 6:
Step 1: Example 6f
[00290] To a solution of Example 6i (2.0 g, 5.6 mmol, 1.0 eq) in toluene (20 mL) was added TBTO (6.7 g, 11.2 mmol, 2.0 eq). The mixture was heated to 110 o C and stirred for 16 h. The mixture was concentrated in vacuo. The crude product was purified by silica gel flash column chromatography to afford the desired product Example 6f (1.7 g, yield 88.5%) as a yellow solid. LCMS [M+1] + =327.2. Step 2: Example 6c
[00291] To a solution of Example 6b (1.18 g, 7.34 mmol, 1.2 eq) in DMF (10 mL) was added NaH (539 mg, 60% in mineral oil, 13.5 mmol, 2.2 eq) in portions at 0 o C. After stirring for 0.5 h, a solution of Example 6a (1.5 g, 6.12 mmol, 1.0 eq) in DMF (20 mL) was added dropwise. The reaction mixture was stirred at r.t. for 2 h. The reaction was quenched with saturated NH 4 Cl aqueous (50 mL) at 0 o C and extracted with EtOAc (100 mL*3). The combined organic layer was washed with brine (50 mL*3), dried over Na 2 SO 4 and concentrated in vacuo. The crude product was purified by silica gel flash column chromatography to afford the desired product Example 6c (1.1 g, yield 55.2%) as a yellow solid. LCMS [M+1] + =327.3.
Step 3: Example 6d
[00292] Example 6c (500 mg, 1.53 mmol, 1.0 eq) was dissolved with MeOH (10 mL) and 5% Pd/C (100 mg) was added under N2 protection. The system was evacuated and then refilled with hydrogen. The mixture solution was stirred for 1 h at r.t. under H 2 balloon. The reaction mixture was filtered and the filtrate was concentrated to afford the desired product Example 6d (450mg, yield 99.3%) as colorless oil. LCMS [M+1] + =297.3.
Step 4: Example 6e [00293] To a solution of Example 6d (450 mg, 1.52 mmol, 1.0 eq) in DCM (10 mL) was added HCl/dioxane (3 mL, 4M). The reaction mixture was stirred for 1 h at r.t. The reaction solution was concentrated in vacuo to afford the desired product Example 6e (300 mg, yield 85.2%) as a white solid. LCMS [M+1] + =197.3.
Step 5: Example 6g
[00294] To a solution of Example 6f (320 mg, 0.98 mmol, 1.0 eq) in DCM (15 mL) were added DIEA (760 mg, 5.88 mmol, 6.0 eq) and HATU (448 mg, 1.17 mmol, 1.2 eq). After stirred for 0.5 h, Example 6e (316 mg, 1.17 mmol, 1.2 eq) was added. The reaction solution was stirred for 2 h at r.t. The solvent was removed, and the residue was purified by silica gel flash column chromatography to afford the desired product Example 6g (220 mg, yield 44.4%) as a yellow solid. LCMS [M+1] + =505.4.
Step 6: Example 6h
[00295] To a solution of Example 6g (170 mg, 0.33 mmol, 1.0 eq) in dioxane (10 mL) were added Cs 2 CO 3 (219 mg, 0.67 mmol, 2.0 eq) and 3 rd -t-Bu-Xphos Pd (30 mg, 0.033 mmol, 0.1 eq). The reaction mixture was stirred for 2 h at 80 o C under N2. The reaction solution was concentrated in vacuo. The crude product was purified by Prep-TLC to afford the desired product Example 6h (110 mg, yield 69.6%) as a yellow solid. LCMS [M+1] + =469.4.
Step 7: Example 6
[00296] To a solution of Example 6h (110 mg, 0.17mmol, 1.0 eq) in DCM (5 mL) was added
HCl/dioxane (1 mL, 4 M in dioxane) at 0 o C. The solution was stirred for 0.5 h at r.t. and then concentrated. The crude product was dissolved in MeOH and Na2CO 3 (aq.) was added to basified pH ~8. The mixture was concentrated and the residue was purified by prep-TLC to afford the desired product Example 6 (55 mg, yield 63.6%) as an off-white solid. LCMS [M+1] + =369.4. 1 H NMR (300 MHz, DMSO-d6) d 8.76 (brs, 1H), 8.42 (s, 1H), 8.07 (d, 1H), 7.81 (s, 1H), 7.45-7.37 (m, 1H), 7.01 (d, 1H), 6.90 (dd, 1H), 6.22 (s, 1H), 4.50 (s, 2H), 3.88 (s, 3H), 3.57-3.54 (m, 2H), 3.45-3.37 (m, 2H), 2.89 (d, 3H). Example 7:
Step 1: Example 7b
[00297] To a mixture of Example 7a (21.0 g, 0.126 mol) in CCl 4 (400 mL) were added NBS (23.5 g, 0.132 mol) and AIBN (4.1 g, 0.025 mol). The reaction mixture was stirred at 80°C for 16 h. After cooled to room temperature, the solvent was removed, and the residue was purified by silica gel flash column chromatography to afford the product Example 7b (18.5g, yield 59.8%) as a yellow solid.
Step 2: Example 7d
[00298] To a solution of Example 7c (2.13 g, 12.2 mmol)in THF (50 mL) was added NaH (0.81 g, 60% in mineral oil, 20.3 mmol) in portions at 0°C. The mixture was stirred for 10 min at the same temperature, then Example 7b (2.0 g, 8.1 mmol) in THF was added dropwise. The reaction mixture was stirred at r.t. for 3 h. The mixture was quenched with saturated NH 4 Cl aqueous solution (50 mL) and extracted with EtOAc (50 mL*2). The combined organic layer was washed with brine, dried over anhydrous Na 2 SO 4 and concentrated in vacuo. The residue was purified by silica gel flash column chromatography to afford the product Example 7d (1.1 g, yield 39.8) as yellow oil. LCMS [M+1-100] + = 241.2.
Step 3: Example 7e
[00299] To a solution of Example 7d (1.0 g, 2.94 mmol) in MeOH (50 mL) was added 5% Pd/C (100 mg) under N2 protection. The suspension was degassed under vacuum and purged with H 2 for 3 times. The mixture was stirred at r.t. for 2 h under H 2 balloon. The solid was filtered out, and the filtrate was concentrated to afford the product Example 7e (900 mg, yield 98.8%) as yellow oil. LCMS [M+Na] + = 333.4.
Step 4: Example 7f
[00300] To a solution of Example 7e (500 mg, 1.6 mmol) in DCM (10 mL) was added HCl/dioxane (2 mL,4M in dioxane, 8.0 mmol). The reaction mixture was stirred at r.t. for 1 h. The mixture was concentrated to afford the product Example 7f (480 mg, crude, quant.) as yellow oil. LCMS [M+1] + = 211.2.
Step 5: Example 7h
[00301] To a solution of Example 7g (324 mg, 0.99 mmol, from Example 6f) in DCM (20 mL) were added DIEA (1.0 g, 7.95 mmol) and HATU (415mg, 1.1 mmol). After stirring for 10 min, Example 7f (450 mg, 2.14 mmol) was added to the mixture. The reaction mixture was stirred for 2 h at room temperature. After the reaction was completed, the solvent was removed and the crude was purified by silica gel chromatography to give the desired product Example 7h (200 mg, yield 24.3%) as a yellow solid. LCMS [M+1] + =519.2.
Step 6: Example 7i
[00302] To a solution of Example 7h (200 mg, 0.39 mmol) in dioxane (10 mL) were added Cs 2 CO 3 (251 mg, 0.77 mmol) and 3rd-t-Bu-Xphos-Pd (34 mg, 0.04 mmol). The reaction mixture was stirred at 80°C for 3 h under N 2 . After cooled to room temperature, the solvent was removed, and the residue was purified by silica gel flash column chromatography to afford the product Example 7i (105 mg, yield 56.4%) as a yellow solid. LCMS [M+1] + = 483.2.
Step 7: Example 7
[00303] To a solution of Example 7i (100mg, 0.2 mmol) in DCM (5 mL) was added HCl/dioxane (1.0 mL,4M in dioxane, 4.0 mmol). The reaction mixture was stirred at r.t. for 3 h and then concentrated in vacuum. The residue was dissolved in MeOH (5 mL) and basified with NaHCO 3 until pH~8. DCM (100 mL) was added to the mixture. The mixture was filtered through a silica gel column. The filtrate was concentrated to give the desired product Example 7 (38.0 mg, yield 47.9%) as a white solid. LCMS [M+1] + = 383.3. 1 H NMR (300 MHz, DMSO-d 6 ) d 8.78 (d, 1H), 8.44 (s, 1H), 8.15 (d, 1H), 7.81 (s, 1H), 7.41 (d, 1H), 7.02 (d, 1H), 6.89 (dd, 1H), 6.22 (s, 1H), 4.65 (d, 1H), 4.38 (d, 1H), 4.05 - 3.94 (m,1H), 3.89 (s, 3H), 3.48 (dd, 1H), 3.29 - 3.22 (m, 1H), 2.88 (d, 3H), 1.14 (d, 3H).
Example 8:
Step 1: Example 8c
[00304] To a solution of Example 8b (2.13 g, 12.20 mmol, 1.5 eq) in THF (50 mL) was added NaH (813 mg, 60% in mineral oil, 20.33 mmol, 2.5 eq) in portions at 0 o C. After stirred for 30 min, to the above solution was added a solution of Example 8a (2.0 g, 8.13 mmol, 1.0 eq) in THF (10 mL). The reaction mixture was stirred at r.t. for 2 h. The reaction was quenched with saturated NH 4 Cl aqueous solution (25 mL) at 0oC and extracted with EtOAc (50 mL*3). The combined organic layer was washed with brine, dried over Na 2 SO 4 and concentrated. The crude product was purified by silica gel flash column chromatography to afford the desired product Example 8c (980mg, yield 35.4%) as a yellow solid. LCMS [M+1] + = 341.3.
Step 2: Example 8d
[00305] Example 8c (980 mg, 2.88 mmol, 1.0 eq) was dissolved in MeOH (20 mL), 5% Pd/C (500 mg) was added under N 2 protection. The suspension was evacuated and then refilled with hydrogen for three times. The mixture was stirred for 1 h at r.t. under H 2 balloon. The solid was filtered out and the filtrate was concentrated to afford the desired product Example 8d (935 mg, crude, quant.) as a brown solid. LCMS [M+1] + = 311.4.
Step 3: Example 8e
To a solution of Example 8d (835 mg, 2.69 mmol, 1.0 eq) in DCM (12 mL) was added HCl/dioxane (3 mL, 4M in dioxane). The reaction mixture was stirred at r.t. for 2 h. The solvent was concentrated under vacuum to give a crude product Example 8e (980 mg, crude, quant) as a yellow solid. LCMS [M+1] + =211.3
Step 4: Example 8g
[00306] To a solution of Example 8f (300 mg, 0.92 mmol, 1.0 eq, from Example 6f) in DCM (10 mL) were added DIEA (947 mg, 7.34 mmol, 8.0 eq) and HATU (383 mg, 1.01 mmol, 1.1 eq). After stirring for 30 min, Example 8e (340 mg, 1.38 mmol, 1.5 eq) was added to the solution. The reaction was stirred for 2 h at r.t. The solvent was concentrated, and the residue was purified by silica gel flash column chromatography to afford the desired product Example 8g (160 mg, yield 33.6%) as yellow oil. LCMS [M+1] + =519.3.
Step 5: Example 8h
[00307] To a solution of Example 8g (150 mg, 0.29 mmol, 1.0 eq) in dioxane (10 mL) were added Cs 2 CO 3 (188 mg, 0.58 mmol, 2.0 eq) and 3 rd -t-Bu-Xphos-Pd (27 mg, 0.029 mmol, 0.1 eq). The reaction mixture was stirred for 2 h at 80 o C under N2. The reaction solution was filtered and the filtrate was concentrated. The crude product was purified by prep-TLC to afford the desired product Example 8h (90 mg, yield 64.5%) as a yellow solid. LCMS [M+1] + =483.4.
Step 6: Example 8
[00308] To a solution of Example 8h (80 mg, 0.17 mmol, 1.0 eq) in DCM (3 mL) was added
HCl/dioxane (1 mL, 4 M in dioxane) at 0 o C. The reaction was stirred for 30 min at r.t. and then concentrated. The crude product was dissolved in MeOH, Na 2 CO 3 (excess) was added and stirred at r.t. for 10 min. The solid was filtered out, the filtrate was concentrated. The residue was purified by silica gel column chromatography to afford the desired product Example 8 (40.0 mg, yield 63.1%) as an off-white solid. LCMS [M+1] + =383.3. 1 H NMR (300 MHz, DMSO-d 6 ) d 8.73 (s, 1H), 8.42 (s, 1H), 8.10 (d, 1H), 7.81 (s, 1H), 7.40 (d, 1H), 7.00 (d, 1H), 6.92 (dd, 1H), 6.21 (s, 1H), 4.64 (d, 1H), 4.42 (d, 1H), 3.88 (s, 3H), 3.65 - 3.49 (m, 1H), 3.42 - 3.35 (m, 1H), 3.27 - 3.14 (m, 1H), 2.89 (d, 3H), 1.20 (d, 3H).
Example 9:
Step 1: Example 9b
[00309] To a solution of Example 9a (10.0 g, 50.8 mmol, 1.0 eq) in dry THF (100 mL) was added BH3.Me2S (6.1 mL, 10M in DMS, 61.0 mmol, 1.2 eq) dropwise at r.t. The solution was stirred for 3 h at 70 °C. After cooled to room temperature, 3M HCl aqueous solution was added dropwise into the reaction solution until effervescence was no longer observed. The resulting mixture was extracted with EtOAc (100 mL*3). The combined organic layer was washed with saturated Na2CO 3 aqueous, followed by brine, dried over Na 2 SO 4 , and concentrated in vacuum to afford the product Example 9b (8.7 g, yield 94%) as an off-white solid. LCMS [M-18+1] + = 166.2
Step 2: Example 9c
[ 00310] To a solution of Example 9b (2.6 g, 14.2mmol, 1.0 eq) in dry DCM (60 mL) was added PBr 3 (7.7 g, 28.4 mmol, 2.0 eq) dropwise, which was stirred for 2 h at r.t. The reaction was diluted with DCM (100 mL), and Na2CO 3 aqueous solution was added to the solution until a neutral pH was obtained. The resulting mixture was extracted with DCM (100 mL*2). The combined organic layer was washed with brine, dried over Na 2 SO 4 , and concentrated in vacuum to give the product Example 9c (3.3 g, yield 95%) as an off-white solid. LCMS [M+1] + = 246.1.
Step 3: Example 9e
[00311] To a solution of Example 9d (1.47 g, 9.15 mmol, 1.5 eq) in dry THF (10 mL) was added NaH (610 mg, 60% in mineral oil, 15.25 mmol, 2.5 eq) in portions at 0 °C, which was stirred for 30 min. Then a solution of Example 9c (1.50 g, 6.1 mmol, 1.0 eq) in THF (5 mL) was added dropwise. The mixture was stirred for 1 h at r.t., then quenched with water (15 mL), extracted with EtOAc (30 mL*2). The combined organic layer was washed with brine, dried over Na 2 SO 4 , concentrated in vacuum, and the residue was purified by silica gel flash column chromatography to give the desired product Example 9e (1.1g, yield 55%) as yellow oil. LCMS [M+1] + = 327.3.
Step 4: Example 9f
[00312] To a solution of Example 9e (1.1 g, 3.4 mmol, 1.0 eq) in MeOH (25 mL) was added 5% Pd/C (200 mg) under N 2 protection, the suspension was degassed under vacuum and purged with H 2 for three times. The mixture was stirred for 2 h at r.t. under H 2 balloon. The solid was filtered out, and the filtrate was concentrated to give the desired product Example 9f (950 mg, yield 94%) as yellow oil. LCMS [M+1] + = 297.3.
Step 5: Example 9g
[00313] To a solution of Example 9f (400 mg, 1.35 mmol, 1.0 eq) in DCM (10mL) was added HCl/dioxane (4M in dioxane, 2 mL). The solution was stirred for 2 h at r.t. and then concentrated to give the product (650 mg, crude, quant.) as yellow oil. LCMS [M+1] + = 197.3
Step 6: Example 9i
To a solution of Example 9h (250 mg, 0.77 mmol, 1.0 eq, from Example 6f) and DIEA (695.3 mg, 5.39 mmol, 7.0 eq) were added HATU (352 mg, 0.92 mmol, 1.2 eq), which was stirred for 10 min at room temperature. Then Example 9g (452 mg, 2.31 mmol, 3.0 eq) was added. The mixture was stirred for 2 h at r.t., and the solvent was removed. The residue was purified by silica gel flash column chromatography to give the desired product Example 9i (280 mg, yield 72%) as yellow oil. LCMS [M+1] + = 505.3. Step 7: Example 9j
[00314] To a solution of Example 9i (100 mg, 0.20 mmol, 1.0 eq) in dioxane (5 mL) was added Cs 2 CO 3 (130 mg, 0.40 mmol, 2.0 eq) and 3 rd -t-Bu-Xphos-Pd (17.4 mg, 0.02 mmol, 0.1 eq). The reaction mixture was stirred for 4 h at 110 o C under N2. After cooled to room temperature, the reaction solution was filtered and the filtrate was concentrated. The crude product was purified by prep-TLC to afford the desired product Example 9j (30 mg, yield 32%) as a yellow solid. LCMS [M+1] + =469.2.
Step 8: Example 9
[00315] To a solution of Example 9j (20 mg, 0.043 mmol, 1.0 eq) in DCM (6 mL) was added HCl/dioxane (2 mL, 4M in dioxane), which was stirred for 1 h at r.t. and then concentrated. The residue was diluted with MeOH (5 mL), and K 2 CO 3 (excess) was added. The mixture was stirred for 30 min at r.t. The solid was filtered out, the filtrate was concentrated and the residue was purified by Prep-TLC to give the desired product Example 9 (10.5 mg, yield 66%) as a white solid. LCMS [M+1] + =369.3. 1 H NMR (300 MHz, DMSO-d6) d 9.88 (s, 1H), 7.78 (s,1H), 7.58 (s,1H), 7.48 (d, 1H), 7.26 (t, 1H), 7.16 (d, 1H), 7.03 (d, 1H), 6.08 (s, 1H), 4.87(s, 2H), 3.80 (s, 5H), 3.63 -3.53 (m, 2H), 2.92 (d, 3H).
Example 10:
Step 1: Example 10c
[00316] To a solution of Example 10b (1.85 g, 10.6 mmol) in THF (20 mL) was added NaH (718 mg, 60% in mineral oil, 17.9 mmol) in portions at 0 o C. After stirring for 0.5 h, a solution of Example 10a (2.0 g, 8.16 mmol, from Example 7b) in THF (10 mL) was added dropwise. The reaction mixture was stirred for 2 h at r.t. The reaction was quenched with saturated NH 4 Cl aqueous (50 mL) at 0 o C and extracted with EtOAc (100 mL*3). The combined organic layer was washed with brine (50 mL*3), dried over Na 2 SO 4 and concentrated in vacuo. The crude product was purified by silica gel flash column chromatography to afford the desired product Example 10c (2.5 g, yield 89.9%) as a yellow solid. LCMS [M+1] + = 341.3.
Step 2: Example 10d
[00317] Example 10c (2.5 g, 7.35 mmol) was dissolved in MeOH (30 mL),and 5% Pd/C (250 mg) was added under N 2 protection. The system was evacuated and then refilled with hydrogen for three times. The mixture solution was stirred for 1 h at r.t. under H 2 balloon. The reaction mixture was filtered and the filtrate was concentrated to afford the desired product Example 10d (1.5 g, yield 65.8%) as colorless oil. LCMS [M+1] + = 311.3.
Step 3: Example 10e
To a solution of Example 10d (1.0 g, 3.22mmol) in DCM (15 mL) was added HCl/dioxane (2 mL, 4M in dioxane, 8 mmol). The reaction mixture was stirred for 1 h at r.t. The reaction solution was concentrated in vacuo to afford the desired product Example 10e (700 mg, yield 79.3%) as a white solid. LCMS [M+1] + =211.2.
Step 4: Example 10g [00318] To a solution of Example 10f (519 mg, 1.84 mmol, from Example 6f) in DCM (10 mL) were added DIEA (950 mg, 7.38 mmol) and HATU (559 mg, 1.47 mmol). After stirred for 0.5 h, Example 10e (400mg, 1.23 mmol) was added. The reaction solution was stirred for 2 h at r.t. The solvent was removed and the residue was purified by silica gel flash column chromatography to afford the desired product Example 10g (210 mg, yield 32.9%) as a yellow solid. LCMS [M+1] + = 519.3.
Step 5: Example 10h
[00319] To a solution of Example 10g (195 mg, 0.38mmol) in dioxane (30 mL) were added Cs 2 CO 3 (245 mg, 0.75mmol) and 3 rd -t-Bu-Xphos-Pd(33 mg, 0.04 mmol). The reaction mixture was stirred at 85°C for 5 h under N 2 . After cooled to room temperature, the solvent was removed, and the residue was purified by silica gel flash column chromatography to afford the product Example 10h (95 mg, yield 52.3%) as a yellow solid. LCMS [M+1] + = 483.2.
Step 6: Example 10
[00320] To a solution of Example 10h (95 mg, 0.2 mmol) in DCM (5 mL) was added HCl/dioxane(1 mL,4M in dioxane, 4 mmol). The reaction mixture was stirred at r.t. for 5 h and then concentrated in vacuum. The residue was dissolved in MeOH (5 mL), and basified with NaHCO 3 (pH = 8). DCM (100 mL) was added to the mixture and the solid was filtered out. The filtrate was concentrated to give the desired product Example 10 (50.0 mg, yield 66.4%) as a white solid. LCMS [M+1] + = 383.3. 1 H NMR (300 MHz, DMSO-d6) d 8.76 (d, 1H), 8.45 (s, 1H), 8.15 (d, 1H), 7.82 (s, 1H), 7.41 (d, 1H), 7.01 (d, 1H), 6.88 (dd, 1H), 6.23 (s, 1H), 4.62 (d, 1H), 4.35 (d, 1H), 4.05 - 3.93 (m, 1H), 3.89 (s, 3H), 3.54 - 3.46 (m, 1H), 3.25 (t, 1H), 2.88 (d, 3H), 1.12 (d, 3H).
Example 11:
Step 1: Example 11c
[00321] To a solution of Example 11b (2.63 g, 15 mmol) in THF (50 mL) was added NaH (1.0 g, 60% in mineral oil, 25 mmol) in portions at 0 o C. After stirring for 10 min, a solution of Example 11a (2.46 g, 10 mmol, from Example 7b) in THF (10 mL) was added dropwise. The reaction mixture was stirred at r.t. for 3 h. The reaction was quenched with saturated NH 4 Cl aqueous (20 mL) at 0 o C and extracted with EtOAc (50mL), dried over Na 2 SO 4 and concentrated in vacuo. The crude product was purified by silica gel flash column chromatography to afford the desired product Example 11c (2.6 g, yield 76.5%) as a yellow solid. LCMS [M+1] + =341.3.
Step 2: Example 11d
[00322] Example 11c (1.5 g, 4.4 mmol) was dissolved in MeOH (30 mL), and then 5% Pd/C (150 mg) was added under N 2 protection. The system was evacuated and then refilled with hydrogen for three times. The mixture solution was stirred for 2 h at r.t. under H 2 balloon. The reaction mixture was filtered and the filtrate was concentrated to afford the desired product Example 11d (1.35 g, yield 98.7%) as yellow oil. LCMS [M+1] + =311.3.
Step 3: Example 11e
[00323] To a solution of Example 11d (600 mg, 1.9 mmol) in DCM (6 mL) was added HCl/dioxane (2 mL,4M in dioxane, 8 mmol). The reaction mixture was stirred at r.t. for 3 h. The reaction solution was concentrated in vacuo to afford the desired product Example 11e (580 mg, crude, quant.) as yellow oil. LCMS [M+1] + =211.2.
Step 4: Example 11g
[00324] To a solution of Example 11f (418 mg, 1.3 mmol, from Example 6f) in DCM (30 mL) were added DIEA (1.3 g, 10.3 mmol) and HATU (730 mg, 1.9 mmol). After stirring for 0.5 h, Example 11e (580 mg, 2.0 mmol) was added. The reaction mixture was stirred for 2 h at r.t. The solvent was removed, and the residue was purified by silica gel flash column chromatography to afford the desired product Example 11g (240 mg, yield 36.1%) as a yellow solid. LCMS [M+1] + =519.3.
Step 5: Example
[00325] To a solution of Example 11g (240 mg, 0.46 mmol) in dioxane (10 mL) were added Cs 2 CO 3 (302 mg, 0.92 mmol) and 3 rd -t-Bu-Xphos-Pd(41 mg, 0.05 mmol). The reaction mixture was stirred at 85°C for 5 h under N2. After cooled to room temperature, the solvent was removed, and the residue was purified by silica gel flash column chromatography to afford the product Example 11h (140 mg, yield 62.7%) as a yellow solid.
LCMS [M+1] + = 483.2.
Step 6: Example 11
[00326] To a solution of Example 11h (140 mg, 0.29 mmol) in DCM (5 mL) was added HCl/dioxane (1 mL, 4M in dioxane, 4 mmol). The reaction mixture was stirred at r.t. for 5 h and then concentrated in vacuum. The residue was dissolved in MeOH (5 mL) and basified with NaHCO 3 (pH = 8). DCM (100 mL) was added to the mixture. The solid was filtered out, and the filtrate was concentrated to give the desired product Example 11 (70.0 mg, 66.4% yield) as an off-white solid. LCMS [M+1] + = 383.3. 1 H NMR (300 MHz, DMSO-d 6 ) d 8.76 (d, 1H), 8.45 (s, 1H), 8.15 (d, 1H), 7.82 (s, 1H), 7.43 (d, 1H), 7.01 (d, 1H), 6.89 (dd, 1H), 6.23 (s, 1H), 4.64 (d, 1H), 4.37 (d, 1H), 4.05 - 3.92 (m,1H), 3.89 (s, 3H), 3.46 (dd, 1H), 3.25 (t, 1H), 2.89 (d, 3H), 1.13 (d, 3H).
Example 12:
Step 1: Example 12b
[ 00327] To a solution of Example 12a (30.0 g, 179 mmol) in CCl 4 (150 mL) were added BPO (4.4 g, 17.9 mmol), NBS (38.15 g, 216 mmol), which was stirred at 80 o C overnight. After cooling, the mixture was then diluted by DCM, washed by water, dried over Na 2 SO 4 , and concentrated under reduced pressure to give Example 12b (37.0 g, yield 84.4%) as a yellow solid, which was used for the next step without purification. LCMS [M+1] + = 246.0. 1 H NMR (400 MHz, Chloroform-d) d 7.87 (d, 1H), 7.57 (dd, 1H), 7.07 (d, 1H), 4.46 (s, 2H), 3.96 (d, 3H).
Step 2: Example 12d
[00328] To a solution of Example 12b (2.46 g, 10.0 mmol) in THF (20 mL) was added NaH (400 mg, 60% in mineral oil, 10.0 mmol) at 0 o C, which was stirred for 0.5 h. Then Example 12c (1.75 g, 10.0 mmol) was added, and the resulting mixture was stirred at r.t. for 6 h. The mixture was quenched by NH 4 Cl aq, extracted by EtOAc, dried over Na 2 SO 4 , and concentrated under reduced pressure. The residue was purified by silica gel column chromatography to give Example 12d (3.3 g, yield 96.8%) as a yellow solid. LCMS [M+1-100] + = 241.1.
Step 3: Example 12e
[00329] A suspension of Example 12d (688 mg, 2.0 mmol) and 10% Pd/C (34 mg) in MeOH (10 mL) was stirred at r.t. for 2 h under H 2 balloon. The suspension was filtered, and the filtrate was concentrated under reduced pressure to give Example 12e (640 mg, crude yield 103%) as a yellow solid, which was used for the next step without purification.
Step 4: Example 12f
[00330] To a solution of Example 12e (400 mg, 1.3 mmol) in dioxane (2 mL) was added HCl/dioxane (1.0 mL, 4M in dioxane), which was stirred at r.t. for 2 h. The mixture was concentrated, and the residue was treated with EtOAc (30 mL) to give the crude product Example 12f (340 mg, crude yield 124%) as a white solid, which was used for the next step without purification.
Step 5: Example 12h
[00331] To a solution of Example 12f (340 mg, 0.65 mmol), Example 12g (423 mg, 1.3 mmol, from Example 6f), and TEA (810 mg, 8.1 mmol) in DCM (10 mL) was added HATU (616 mg, 1.62mmol). The mixture was stirred at r.t. for 1 h. EtOAc (40 mL) was added to the reaction mixture, which was washed with brine (20 mL*2), dried over Na 2 SO 4 and concentrated. The residue was purified by silica gel column chromatography to afford the desired product Example 12h (500 mg, yield 59%) as a white solid. LCMS [M+1] + = 519.2.
Step 6: Example 12i
[00332] To a mixture of Example 12h (500 mg, 0.97 mmol,), Cs 2 CO 3 (652 mg, 2.0 mmol) in dioxane (10 mL) was added 3rd-t-Bu-Xphos-Pd (89 mg, 0.1 mmol). The mixture was degassed with N2 three times, and stirred for 3 h at 80°C. The mixture was diluted by DCM, washed by water, dried over Na 2 SO 4 , and concentrated under reduced pressure to give Example 12i (450 mg, crude yield 93.3%) as a white solid, which was used for the next step without purification. LCMS [M+1] + = 483.3
Step 7: Example 12
[00333] To a solution of Example 12i (200 mg, 0.42 mmol) in dioxane (2 mL) was added HCl/dioxane (1.0 mL, 4M in dioxane), which was stirred at r.t. for 2 h. The mixture was concentrated, and the residue was purified by Prep-HPLC to afford the desired product Example 12 (4.9 mg, yield 3.0%) as a white solid. LCMS [M+1] + = 383.3. 1 H NMR (400 MHz, DMSO-d6) d 8.90 (s, 1H), 8.32 (s, 1H), 8.22 (d, 1H), 8.08 (s, 1H), 7.80 (d, 1H), 6.97 (d, 1H), 6.88 (d, 1H), 5.91 (s, 1H), 4.54 (d, 1H), 4.37 (d, 1H), 3.85 (s, 3H), 3.44 (d, 1H), 2.89 (d, 3H), 1.11 (d, 3H).
Example 13:
Step 1: Example 13b
[00334] To a solution of Example 13a (10.0 g, 0.05 mol) in MeOH (150 mL) was added NaBH4 (4.87 g, 0.13 mol) in portions. The reaction mixture was stirred at r.t. for 2 h. The solvent was removed and the residue was purified by silica gel flash column chromatography to afford the product Example 13b (8.5 g, yield 84.1%) as yellow oil. LCMS [M+1] + = 198.2.
Step 2: Example 13c
[ 00335] To a solution of Example 13b (1.97 g, 10.0 mmol) in DCM (50 mL) was added PBr 3 (5.4 g, 20.0 mmol). The reaction mixture was stirred at r.t. for 3 h. The mixture was diluted with DCM (100 mL) and washed with saturated NaHCO 3 aqueous (50 mL*2). The organic layer dried over anhydrous Na 2 SO 4 and concentrated in vacuo to afford the product Example 13c (2.3 g, yield 88.5%) as yellow oil.
Step 3: Example 13e
[00336] To a solution of Example 13d (2.0 g, 12.5 mmol) in THF (50 mL) was added NaH (0.5 g, 60% in mineral oil, 12.5 mmol) in portions at 0°C. The mixture was stirred for 10 min at the same
temperature, then Example 13c (1.3 g, 5.0 mmol) in THF was added dropwise. The reaction mixture was stirred at r.t. for 3 h. The mixture was quenched with saturated NH 4 Cl (30 mL) and extracted with EtOAc (50 mL). The organic layer was dried over anhydrous Na 2 SO 4 and concentrated in vacuo. The residue was purified by silica gel flash column chromatography to afford the product Example 13e (810 mg, yield 47.6%) as yellow oil. LCMS [M+1-100] + = 241.2.
Step 4: Example 13f
[00337] Example 13e (800 mg, 2.4 mmol) was mixed with MeOH (30 mL), and 5% Pd/C (150 mg) was added under N 2 protection. The system was evacuated and then refilled with hydrogen for three times. The mixture was stirred for 2 h at r.t. under H 2 balloon. The reaction mixture was filtered and the filtrate was concentrated. The residue was purified by silica gel flash column chromatography to afford the product Example 13f (420 mg, yield 57.6%) as yellow oil. LCMS [M+1] + =311.3.
Step 5: Example 13g [00338] To a solution of Example 13f (400 mg, 1.29 mmol) in DCM (5 mL) was added HCl/dioxane (2 mL, 4M in dioxane, 8 mmol). The reaction mixture was stirred for 1 h at r.t. The reaction solution was concentrated in vacuo to afford the desired product Example 13g (360 mg, yield 98.6%) as yellow oil. LCMS [M+1] + =211.2.
Step 6: Example 13i
[00339] To a solution of Example 13h (238 mg, 0.7 mmol, from Example 6f) in DCM (20 mL) were added DIEA (752 mg, 5.8 mmol) and HATU (443 mg, 1.2 mmol). The solution was stirred for 0.5 h, then Example 13g (330 mg, 1.2 mmol) was added. The reaction solution was stirred for 2 h at r.t. The solvent was removed, and the residue was purified by silica gel flash column chromatography to afford the desired product Example 13i (41 mg, yield 10.8%) as a yellow solid. LCMS [M+1] + =519.3.
Step 7: Example 13j
[00340] To a solution of Example 13i (41 mg, 0.08 mmol) in 1,4-dioxane (10 mL) were added Cs 2 CO 3 (51 mg, 0.16 mmol) and 3rd-t-Bu-Xphos-Pd (7 mg, 0.01 mmol). The reaction mixture was stirred at 85°C for 4 h under N2. After cooled to room temperature, the solvent was removed, and the residue was purified by Prep-TLC to afford the product Example 13j (25 mg, yield 65.5%) as a yellow solid. LCMS [M+1] + = 483.2.
Step 8: Example 13
[00341] To a solution of Example 13j (25 mg, 0.05 mmol) in DCM (3 mL) was added HCl/dioxane (0.2 mL, 4M in dioxane, 0.8 mmol). The reaction mixture was stirred at r.t. for 2 h and then concentrated in vacuum. The residue was dissolved in MeOH (5 mL), Then pH valve was adjusted to 8 with saturated NaHCO 3 aqueous. The solvent was removed, and the residue was purified by Prep-TLC to afford the product Example 13 (11.8 mg, yield 59.6%) as an off-white solid. LCMS [M+1] + = 383.3. 1 H NMR (300 MHz, DMSO-d6) d 8.80 (s, 1H), 8.42 (s, 1H), 8.03 (d, 1H), 7.80 (s, 1H), 7.41 (d, 1H), 7.00 (d, 1H), 6.90 (dd, 1H), 6.21 (s, 1H), 4.45 (q, 1H), 3.88 (s, 3H), 3.75-3.71 (m, 1H), 3.53-3.43 (m, 1H), 3.42-3.37 (m, 1H), 3.26-3.23 (m, 1H), 2.89 (d, 3H), 1.30 (d, 3H).
Example 14:
Step 1: Example 14b
[00342] A solution of Example 14a (15.0 g, 87.2 mmol) and MeONa (14.1 g, 261.6 mmol) in MeOH (100 mL) was stirred at 70 o C for 3 h. The mixture was concentrated under reduced pressure, and then diluted by water, which was then extracted by EtOAc, dried over anhydrous Na 2 SO 4 , and concentrated to afford crude product Example 14b (13.4 g, yield: 92.2%) as a yellow solid. The residue was used in the next step directly without further purification. LCMS [M+1] + = 169.1.
Step 2: Example 14c
[00343] To a solution of Example 14b (5.0 g, 29.8 mmol) in CCl 4 (150 mL) were added BPO (720 mg, 2.98 mmol), and NBS (5.3 g, 29.8 mmol). The reaction mixture was stirred at 80 o C overnight, and then diluted by DCM, washed by water, dried over anhydrous Na 2 SO 4 . After filtration, the filtrate was concentrated under reduced pressure, which was then purified by silica gel column chromatography to give Example 14c (5.7 g, yield: 77.6%) as a yellow solid. LCMS [M+1] + = 247.0
Step 3: Example 14e
[00344] To a solution of Example 14d (2.1 g, 12.1 mmol) in THF (40 mL) was added NaH (1.46 g, 36.4 mmol) at 0 o C. The reaction mixture was warmed to room temperature and stirred at r.t. for 0.5 h. Then Example 14c (3.0 g, 12.1 mmol) was added. The mixture was stirred at r.t. for 6 h, which was then quenched by aq. NH 4 Cl, extracted by EtOAc, and dried over anhydrous Na 2 SO 4 . After filtration, the filtrate was concentrated under reduced pressure, which was then purified by silica gel column chromatography to give Example 14e (1.0 g, yield: 24.4%) as a yellow solid. LCMS [M-174] + = 167.1. Step 4: Example 14f
[00345] A solution of Example 14e (1.0 g, 2.93 mmol) and Pd/C (200 mg) in MeOH (5 mL) was stirred at r.t. for 2 h under 1 atm of H 2 . After filtration, the filtrate was concentrated under reduced pressure to afford Example 14f (850 mg, yield: 93.2%) as a yellow solid, which was used in next step directly. LCMS [M-174] + = 137.1 Step 5: Example 14g
[00346] To a solution of Example 14f (800 mg crude, 1.3 mmol) in DCM (4 mL) was added TFA (1.0 mL), which was stirred at r.t. for 2 h. The mixture was concentrated to give the crude product Example 14g (700 mg, crude, yield: quant.) as black oil. LCMS [M-74] + = 137.1.
Step 6: Example 14i
[00347] To a solution of Example 14g (30 mg, 0.1 mmol), Example 14h (33 mg, 0.1 mmol, from Example 6f), TEA (202 mg, 1.0 mmol) in DCM (2 mL) was added HATU (38 mg, 0.1 mmol). The reaction mixture was stirred at r.t. for 2 h. Then EtOAc (40 mL) was added to the reaction mixture, which was washed with brine (20 mL*2), dried over anhydrous Na 2 SO 4 and concentrated. The residue was purified by silica gel column chromatography to afford the desired product Example 14i (32 mg, yield: 62%) as a brown solid. LCMS [M+1] + = 520.2.
Step 7: Example 14j
[00348] To a mixture of Example 14i (32 mg, 0.06 mmol,), Cs 2 CO 3 (30 mg, 0.09 mmol) in dioxane (2 mL) was added 3rd-t-Bu-Xphos-Pd (5.5 mg, 0.006 mmol). The mixture was degassed with N2 three times, and stirred for 3 h at 80°C. Then the reaction mixture diluted by DCM, washed by water, dried over anhydrous Na 2 SO 4 , and then concentrated under reduced pressure to afford crude Example 14j (50 mg, crude, yield: quant.) as a white solid, which was used in next step without further purification.
LCMS [M+1] + = 484.2
Step 8: Example 14
[00349] To a solution of Example 14j (50 mg, 0.1 mmol) in DCM (4 mL) was added TFA (1.0 mL), which was stirred at r.t. for 2 h. The mixture was concentrated, and the residue was purified by Prep- HPLC to afford the desired product Example 14 (4.5 mg, yield: 31.4%) as a white solid. LCMS [M+1] + = 384.2. 1 H NMR (400 MHz, Chloroform-d) d 8.58 (s, 1H), 8.53 (s, 1H), 8.08 (s, 1H), 7.67 (s, 1H), 6.69 (s, 1H), 5.67 (s, 1H), 4.68 (d, 1H), 4.45 (d, 1H), 4.24 (br,1H), 4.05 (s, 3H), 3.57 - 3.54 (m, 1H), 3.39 - 3.34 (m, 1H), 3.03 (d, 3H),1.25 (d, 3H).
Example 15:
Step 1: Example 15b
[00350] To a solution of Example 15a (15.0 g, 87.2 mmol) in CCl 4 (500 mL) were added NBS (31.0 g, 174.4 mmol) and AIBN (2.86 g, 17.4 mmol). The reaction mixture was stirred at 80°C for 20 h under N 2 . After filtration, the filtrate was concentrated, and the residue was purified by silica gel flash column chromatography to afford the product Example 15b (7.5 g, yield: 34.2%) as yellow oil. LCMS [M+1] + = 252.9.
Step 2: Example 15d
[00351] To a solution of Example 15c (5.8 g, 33.4 mmol) in THF (250 mL) was added NaH (1.3 g, 60% in mineral oil, 33.4 mmol) in portions at 0°C. The mixture was stirred for 5 min at the same temperature, then Example 15b (7.0 g, 27.8 mmol) in THF was added dropwise. The reaction mixture was stirred at r.t. for 1 h. After the solvent was concentrated, the residue was purified by silica gel flash column chromatography to afford the product Example 15d (2.6 g, yield: 30.0%) as yellow oil. LCMS [M+1] + = 346.2.
Step 3: Example 15e
[00352] To a mixture of Example 15d (2.5 g, 7.2 mmol) in H 2 O (50 mL) was added NaOH (1.2 g, 28.9 mmol). The mixture was stirred at 50°C for 16 h. After cooled to room temperature, the reaction solution was concentrated in vacuo to afford the desired product Example 15e (3.7 g, crude, yield: quant.) as a yellow solid. LCMS [M+1] + = 328.3.
Step 4: Example 15f
[00353] To a solution of Example 15e (3.7 g, crude, 7.2 mmol) in DMF (50 mL) was added CH3I (2.4 g,17.0 mmol). The reaction mixture was stirred at r.t. for 6 h. After concentration, the residue was diluted with EtOAc (100 mL), washed with H 2 O (100 mL), dried over anhydrous Na 2 SO 4 and concentrated. The residue was purified by silica gel flash column chromatography (THF/Petroleum Ether = 4/1) to afford the product Example 15f (860 mg, yield: 35.0% for 2 steps) as yellow oil. LCMS [M+1] + = 342.2. Step 5: Example 15g
[00354] Example 15f (820 mg, 2.4 mmol) was dissolved in MeOH (20 mL) and then Pd/C (80 mg) was added in portions under N 2 protection. The mixture was degassed under vacuum and purged with H 2 for three times. The mixture was stirred for 2 h at r.t. under H 2 balloon. The solid was filtered off and the filtrate was concentrated. The residue was purified by silica gel flash column chromatography to afford the product Example 15g (380 mg, yield: 50.8%) as yellow oil. LCMS [M+1] + = 312.2.
Step 6: Example 15h
[00355] To a solution of Example 15g (370 mg, 1.2 mmol) in DCM (10 mL) was added TMSOTf (396 mg, 1.8 mmol) at 0°C. The reaction mixture was stirred at r.t. for 1 h. The solvent was concentrated in vacuo to afford the desired product Example 15h (430 mg, crude) as yellow oil. LCMS [M+1] + = 212.2. Step 7: Example 15j
[00356] To a solution of Example 15i (260 mg, 0.8 mmol, from Example 6f) in DCM (20 mL) were added DIEA (411 mg, 3.2 mmol) and HATU (303 mg, 0.8 mmol). The mixture was stirred for 5 min, and then Example 15h (420 mg, crude, 2.0 mmol) was added. The resulting mixture was stirred for 2 h at r.t. The solvent was removed and the residue was purified by silica gel flash column chromatography to afford the desired product Example 15j (200 mg, 30.6% yield) as a yellow solid. LCMS [M+1] + =520.2. Step 8: Example 15k
[00357] To a solution of Example 15j (190 mg, 0.37 mmol) in dioxane (20 mL) were added K 2 CO 3 (101 mg, 0.73 mmol), BINAP (228 mg, 0.37 mmol) and Pd2(dba)3CHCl 3 (189 mg, 0.18 mmol). The reaction mixture was stirred at 80°C for 16 h under N2. After cooled to room temperature, the solvent was removed, and the residue was purified by Pre-TLC to afford the product Example 15k (50 mg, 28.3% yield) as a yellow solid. LCMS [M+1] + = 484.4.
Step 9: Example 15
[00358] To a solution of Example 15k (45 mg, 0.09 mmol) in DCM (5 mL) was added TMSOTf (41 mg, 0.02 mmol) at 0°C. The reaction mixture was stirred at r.t. for 2 h. The reaction solution was concentrated in vacuo and the residue was purified by Pre-TLC to afford the product Example 15 (15.3 mg, yield: 42.9%) as an off-white solid. LCMS [M+1] + = 384.3. 1 H NMR (300 MHz, DMSO-d6) d 8.73 - 8.63 (m, 2H), 8.10 (d, 1H), 7.83 (s, 1H), 7.46 (q, 1H), 7.33 (d, 1H), 6.34 (s, 1H), 4.47 (d, 1H), 4.23 (d, 1H), 4.05 - 4.03 (m, 1H), 3.53 (s, 3H), 3.48(d, 1H),3.40 (d, 1H), 2.87 (d, 3H), 1.13 (d, 3H).
Example 16
Step 1：2-fluoro-4-methoxy-5-nitro-benzaldehyde (16B)
[00359] 2-fluoro-4-methoxy-benzaldehyde (16A) (5 g, 32.46 mmol) was dissolved in concentrated sulfuric acid (30 mL) and cooled to -10°C. Concentrated nitric acid (2.1 mL) in concentrated sulfur acid (4 mL) was added dropwise over 20 min. After an additional hour of stirring at below -10°C, the mixture was poured into crushed ice. the precipitate was collected by filtration and partitioned between dichloromethane (40 mL) and saturated sodium hydrogen carbonate (30 mL). The organic layer was dried (Na 2 SO 4 ) and evaporated in vacuo to give the title compound (16 B) (5.2g, 80.50%) as a cream solid. LC-MS (ESI): m/z =200.1 [M+H] + .
Step 2：(2-fluoro-4-methoxy-5-nitro-phenyl)methanol (16C)
[00360] Sodium borohydride (0.304 g,8.04 mmol) was added portionwise to a stirring solution of 2- fluoro-4-methoxy-5-nitro-benzaldehyde (16B)(0.8 g,4.02 mmol) in methanol (10mL) at 0°C. After 2 hours, the methanol was removed in vacuo. The residue was treated with cold water and extracted with dichloromethane. The combined organic layer was washed with brine, dried (Na 2 SO 4 ) and then evaporated in vacuo to give the title compound (16C) as a crude solid (0.79 g, 97.77%). LC-MS (ESI): m/z =202.1 [M+H] +
Step 3：1-(bromomethyl)-2-fluoro-4-methoxy-5-nitro-benzene (16D)
[00361] Carbon tetrabromide (2.64 g,7.96 mmol) in anhydrous diethyl ether (5 mL) was added dropwise to a stirred solution of (2-fluoro-4-methoxy-5-nitro-phenyl)methanol (16C) (0.8 g,3.98 mmol) and triphephosphine (2.08 g,7.96 mmol) in anhydrous diethyl ether (15 mL). The mixture was stirred overnight before it was concentrated. chromatography with ethyl acetate in hexane (0-10%) gave the title compound (16D) as pale yellow solid (0.69 g, 66.34%). LC-MS (ESI): m/z =264.1 [M+H] + Step 4：tert-butyl N-[2-[(2-fluoro-4-methoxy-5-nitro-phenyl)methoxy]-1-methyl-e thyl] carbamate (16E)
[00362] Sodium hydride (105 mg, 2.62 mmol) was added portionwise to a stirred solution of tert-butyl N-(2-hydroxy-1-methyl-ethyl)carbamate (0.46 g, 2.62 mmol) in THF (15 mL) at 0°C, the mixture was stirred at 0°C for 10 min. then 1-(bromomethyl)-2- fluoro-4-methoxy-5-nitro-benzene (16D)(0.69 g,2.62 mmol) was added to the mixture at 0°C,after 30 min, The mixture was treated with cold water and extracted with ethyl acetate. The combined organic layer was washed with brine, dried (Na 2 SO 4 ) and then the residue was purified by flash chromatography to afford the title compound (16E)(0.1 g, 10.65%) as a brown solid. LC-MS (ESI): m/z =381.1 [M+23] + . 1 H NMR (400 MHz, CDCl 3 ) d 8.02 (d, 1H), 6.78 (d, 1H), 4.60 (s, 1H), 4.53 (q, 2H), 3.96 (s, 3H), 3.91– 3.83(m, 1H), 3.49– 3.43 (m, 2H), 1.44 (s, 9H), 1.18 (d, 3H).
Step 5：[2-[(5-amino-2-fluoro-4-methoxy-phenyl)methoxy]-1-methyl -ethyl]ammonium;2,2,2- trifluoroacetate (16F)
[00363] Trifluoroacetic acid (1 mL) was added to a solution of N-[2-[(2-fluoro-4-methoxy- 5-nitro- phenyl)methoxy]-1-methyl-ethyl]carbamate(16E)(0.1 g, 0.28mmol) in DCM (3 mL), The mixture was stirred 2 h, The mixture solution was evaporated to dryness, then the title compound (16F) (0.1 g.100%) was obtained as brown liquid, which was used in the next step without further purification. LC-MS (ESI): m/z =259.2 [M+H] +
Step 6：tert-butyl N-[6-chloro-3-[[2-[(2-fluoro-4-methoxy-5-nitro-phenyl)methox y]-1-methyl-ethyl] carbamoyl]imidazo[1,2-b]pyridazin-8-yl]carbamate (16G)
[00364] [2-[(5-amino-2-fluoro-4-methoxy-phenyl)methoxy]-1-methyl-eth yl]ammonium;2,2,2- trifluoroacetate (16F)(0.1g, 0.27 mmol) was dissolved in DMF (5 mL), HATU (0.153 g, 0.4 mmol), DIPEA( 0.07 g, 0.54 mmol) and intermediate 1 (0.09 g, 0.27 mmol) were added to the solution in room temperature. After 18 h, the solution mixture was diluted with EA (30 mL), washed with water (2× 30 mL) and brine (30 mL), dried with Na 2 SO 4 and concentrated. The crude product was purified by flash chromatography (PE/EA = 3:1) to afford the title compound (16G) (0.06 g, 39.47%) as a white solid. LC- MS (ESI): m/z =567.2 [M+H] +
Step 7：tert-butyl N-[3-[[2-[(5-amino-2-fluoro-4-methoxy-phenyl)methoxy]-1-meth yl- ethyl]carbamoyl]-6-chloro-imidazo[1,2-b]pyridazin-8-yl]-N-me thyl-carbamate (H)
[00365] tert-butyl N-[6-chloro-3-[[2-[(2-fluoro-4-methoxy-5-nitro-phenyl)methox y] -1-methyl- ethyl]carbamoyl]imidazo[1,2-b]pyridazin-8-yl]carbamate (16G) (0.06 g, 0.1 mmol) was dissolved in ethanol (9 mL) and H 2 O (3 mL),Fe powder (60 mg,1.06 mmol) and NH 4 Cl(34 mg,0.64 mmol) were added to solution, then the reaction mixture heated to 85 °C for 3 h, After cooling to room temperature, reaction filtered, filtrate was removed in vacuo. The residue was purified by flash chromatography to afford the title compound (16H) (0.044 g, 78.57%) as a white solid. LC-MS (ESI): m/z =537.1 [M+H] + Step 8：tert-butyl (E)-(3 4 -fluoro-3 6 -methoxy-7-methyl-9-oxo-5-oxa-2,8-diaza-1(6,3)-imidazo [1,2- b]pyridazina-3(1,3)-benzenacyclononaphane-1 8 -yl)(methyl)carbamate (16I) [00366] To a solution of (16H) (44 mg, 0.082 mmol) in 1,4-dioxane ( 20 mL) were added Cs 2 CO 3 (80 mg, 0.25 mmol) and 3rd-t-Bu-Xphos-Pd (30 mg). The reaction mixture was stirred at 85°C for 2 h under N 2 . After cooled to room temperature, the solvent was removed, and the residue was purified by silica gel flash column chromatography to afford the product (16I) (22 mg,53.65%) as a white solid. LC-MS (ESI): m/z =501.3 [M+H] +
Step 9：tert-butyl (E)-(3 4 -fluoro-3 6 -methoxy-7-methyl-9-oxo-5-oxa-2,8-diaza-1(6,3)-imidazo [1,2- b]pyridazina-3(1,3)-benzenacyclononaphane-1 8 -yl)(methyl)carbamate (16)
[00367] A solution of (16I) (22 mg, 0.044 mmol) and trifluoroacetic acid (0.5 mL) in DCM (4 mL) was stirred at room temperature for 2 h. Solvent was evaporated, and the crude product was partitioned between water and DCM. The aqueous layer was basified with NaHCO 3 and extracted with DCM.
Combined organic layers were washed with brine, dried over sodium sulfate, filtered, and evaporated, the residue was purified by silica gel flash column chromatography to afford the product 16 (5 mg, 28.57%) as a white solid.1H NMR (400 MHz, CDCl3) d 8.71 (d, 1H), 8.28 (d, 1H), 8.07 (s, 1H), 6.74– 6.60 (m, 3H), 5.64 (s, 1H), 4.69– 2.59 (m, 2H), 4.28– 4.21 (m, 1H), 3.93 (d, 3H), 3.57 (dd, 1H), 3.43– 3.37 (m, 1H), 3.05 (d, 3H), 1.29 (d, 3H). LC-MS (ESI): m/z =401.2 [M+H] +
Example 17
Step 1：tert-butyl N-[(1R)-2-[(2-fluoro-4-methoxy-5-nitro-phenyl)methoxy]-1-met hyl- ethyl]carbamate (17E)
[00368] Sodium hydride (228 mg, 5.70 mmol) was added portionwise to a stirred solution of tert-butyl N-[(1R)-2-hydroxy-1-methyl-ethyl]carbamate (1 g, 5.70 mmol) in THF (30 mL) at 0°C, the mixture was stirred at 0°C for 10 min then 1-(bromomethyl)-2- fluoro-4-methoxy-5-nitro-benzene (16D)(1.5 g,5.70 mmol) was added to the mixture at 0°C,after 30 min, The mixture was treated with cold water and extracted with ethyl acetate. The combined organic layer was washed with brine, dried (Na 2 SO 4 ) and then the residue was purified by flash chromatography to afford the title compound (17E) (0.57 g, 27.94%) as a white solid. LC-MS (ESI): m/z =359.1 [M+H] +
Step 2：[(1R)-2-[(2-fluoro-4-methoxy-5-nitro-phenyl)methoxy]-1-m ethyl-ethyl]ammonium;2,2,2- trifluoroacetate (17F) [00369] Trifluoroacetic acid (3 mL) was added to a solution of tert-butyl N-[(1R)-2-[(2-fluoro-4- methoxy-5-nitro-phenyl)methoxy]-1-methyl-ethyl]carbamate (17E)(0.57 g, 1.53 mmol) in DCM (8 mL), The mixture was stirred overnight, The mixture solution was evaporated to dryness, then the title compound (17F) (0.54 g.91.21%) was obtained as brown liquid, which was used in the next step without further purification. LC-MS (ESI): m/z =259.2 [M+H] +
Step 3：tert-butyl N-[6-chloro-3-[[(1R)-2-[(2-fluoro-4-methoxy-5-nitro-phenyl)m ethoxy]-1-methyl- ethyl]carbamoyl]imidazo[1,2-b]pyridazin-8-yl]carbamate (17G)
[00370] [(1R)-2-[(2-fluoro-4-methoxy-5-nitro-phenyl)methoxy]-1-methy l-ethyl]ammonium;2,2,2- trifluoroacetate (17F) (0.54 g, 1.45 mmol) was dissolved in DMF (15 mL),HATU (0.827 g, 2.17 mmol), DIPEA(0.374 g, 2.9 mmol) and intermediate 1(0.473 g, 1.45 mmol) were added to the solution in room temperature. After 18h, the solution mixture was diluted with EA (50 mL), washed with water (2×50 mL) and brine (50 mL), dried with Na 2 SO 4 and concentrated. The crude product was purified by flash chromatography to afford the title compound (17G) (0.512 g, 62.36%) as a white solid. LC-MS (ESI): m/z =567.2 [M+H] +
Step 4：tert-butyl N-[3-[[(1R)-2-[(5-amino-2-fluoro-4-methoxy-phenyl)methoxy]-1 -methyl- ethyl]carbamoyl]-6-chloro-imidazo[1,2-b]pyridazin-8-yl]carba mate (17H)
[00371] tert-butyl N-[6-chloro-3-[[(1R)-2-[(2-fluoro-4-methoxy-5-nitro-phenyl)m ethoxy]-1-methyl- ethyl]carbamoyl]imidazo[1,2-b]pyridazin-8-yl]carbamate (17G)(0.512 g, 0.9 mmol) was dissolved in ethanol (50 mL) and H 2 O (15 mL), Fe (506 mg, 9.04 mmol) and NH 4 Cl(290 mg, 5.42 mmol) were added, then the reaction mixture heated to 85 °C for 3 h, After cooling to room temperature, filtered, filtrate was removed in vacuo. The residue was purified by flash chromatography (PE/EA = 3:1) to afford the title compound (17H)(0.44 g, 90.90%) as a white solid. LC-MS (ESI): m/z =537.1 [M+H] +
Step 5：tert-butyl ((7R,E)-3 4 -fluoro-3 6 -methoxy-7-methyl-9-oxo-5-oxa-2,8-diaza-1(6,3)- imidazo[1,2-b]pyridazina-3(1,3)-benzenacyclononaphane-1 8 -yl)(methyl)carbamate (17I)
[00372] To a solution of (17H) (440 mg, 0.82 mmol) in 1,4-dioxane (80 mL) was added Cs 2 CO 3 (802 mg, 2.46 mmol) and 3rd-t-Bu-Xphos-Pd (280 mg). The reaction mixture was stirred at 80°C for 2 h under N 2 . After cooled to room temperature, the solvent was removed, and the residue was purified by silica gel flash column chromatography to afford the desired product (17 I) (230 mg，56.09%) as a white solid. LC-MS (ESI): m/z =501.3 [M+H] + .
Step 6：(E)-3 4 -fluoro-3 6 -methoxy-7-methyl-1 8 -(methylamino)-5-oxa-2,8-diaza-1(6,3)-imidazo[1,2- b]pyridazina-3(1,3)-benzenacyclononaphan-9-one (17)
[00373] A solution of (17) (230 mg, 0.46 mmol) and trifluoroacetic acid (2 mL) in DCM (10 mL) was stirred at room temperature for 2 h. Solvent was evaporated, and the crude product was partitioned between water and DCM. The aqueous layer was quenched with NaHCO 3 and extracted with DCM. Combined organic layers were washed with brine, dried over sodium sulfate, filtered, and evaporated, the residue was purified by silica gel flash column chromatography (PE/EA = 2:1) to afford the product 17 (65mg, 35.32%) as a white solid. 1 H NMR (400 MHz, CDCl 3 ) d 8.74 (d, 1H), 8.30 (d, 1H), 8.05 (s, 1H), 6.70– 6.55 (m, 2H), 6.21 (s, 1H), 5.59 (s, 1H), 4.72– 4.55 (m, 2H), 4.31– 4.21 (m, 1H), 3.92 (s, 3H), 3.59– 3.55 (dd, 1H), 3.44– 3.38 (m, 1H) ,3.04 (d, 3H), 1.29 (d, 3H). LC-MS (ESI): m/z =401.2 [M+H] + . Example 18
Step 1：3-fluoro-4-methoxy-5-nitro-benzaldehyde (18B)
[00374] 3-fluoro-4-methoxy-benzaldehyde (18A) (3.6 g, 23.37 mmol ) was dissolved in concentrated sulfuric acid (30 mL) and cooled to -10°C. Concentrated nitric acid (2.5 mL) in concentrated sulfur acid (4 mL) was added dropwise over 20 min. After an additional hour of stirring at below -10°C, the mixture was poured into crushed ice. the precipitate was collected by filtration and partitioned between dichloromethane (40 mL) and saturated sodium hydrogen carbonate (30mL). The organic layer was dried (Na 2 SO 4 ) and evaporated in vacuo to give the title compound (18B) (2.5 g, 53.76%) as an oil. LC-MS (ESI): m/z =200.1 [M+H] + .
Step 2：(3-fluoro-4-methoxy-5-nitro-phenyl)methanol (18C)
[00375] To a stirring solution of 3-fluoro-4methoxy-5-nitro-benzaldehyde (1 g,5.02 mmol) in methanol (20 mL) was added sodium borohydride (0.38 g,10.04 mmol) portionwise at 0°C. After 2 hours, the methanol was removed in vacuo. The residue was treated with cold water and extracted with
dichloromethane. The combined organic layer was washed with brine, dried (Na 2 SO 4 ) and then evaporated in vacuo to give the title compound (18C)as a crude solid(1 g,99.0%). LC-MS (ESI): m/z =202.1 [M+H] + .
Step 31-(bromomethyl)-3-fluoro-4-methoxy-5-nitro-benzene (18D).
[00376] To a solution of (3-fluoro-4-methoxy-5-nitro-phenyl)methanol (1 g, 4.97 mmol) and triphephosphine (2.61g, 9.95 mmol) in anhydrous diethyl ether (30 mL) was added carbon tetrabromide (3.3 g, 9.95 mmol) in anhydrous diethyl ether (5 mL) dropwise. The mixture was stirred overnight before it was concentrated down to a sticky oil. Silica gel chromatography gave the title compound (18D) as a pale yellow solid (0.95g, 73.07%). LC-MS (ESI): m/z =264.1 [M+H] + Step 4：tert-butyl N-[(1R)-2-[(3-fluoro-4-methoxy-5-nitro-phenyl)methoxy]-1-met hyl- ethyl]carbamate (18 E)
[00377] To a stirred solution of tert-butyl N-(2-hydroxy-1-methyl-ethyl)carbamate (0.63 g, 3.61 mmol) in THF (15 mL) was added sodium hydride (144 mg, 3.61 mmol) portionwise at 0°C, the mixture was stirred at 0°C for 10 min. then 1-(bromomethyl)-3- fluoro-4-methoxy-5-nitro-benzene (18D)(0.95 g, 3.61 mmol) was added to the mixture at 0°C, after 30 min, the mixture was quenched with cold water and extracted with ethyl acetate. The combined organic layer was washed with brine, dried (Na 2 SO 4 ) and then the residue was purified by flash chromatography to afford the title compound (18E)(0.63 g, 48.83%) as a brown solid. LC-MS (ESI): m/z =359.1 [M+H] + .
Step 5：[(1R)-2-[(3-fluoro-4-methoxy-5-nitro-phenyl)methoxy]-1-m ethyl-ethyl]ammonium;2,2,2- trifluoroacetate (18F)
[00378] Trifluoroacetic acid (1.5 mL) was added to a solution of N-[2-[(3-fluoro-4-methoxy- 5-nitro- phenyl)methoxy]-1-methyl-ethyl]carbamate (18E) (0.63g, 1.76 mmol) in DCM(5 mL), The mixture was stirred 2 h, The mixture solution was evaporated to dryness, then the title compound (18F) (0.6 g.
91.46%) was obtained as brown liquid, which was used in the next step without further purification. LC- MS (ESI): m/z =259.2 [M+H] +
Step 6：tert-butyl N-[6-chloro-3-[[(1R)-2-[(3-fluoro-4-methoxy-5-nitro-phenyl)m ethoxy]-1-methyl- ethyl]carbamoyl]imidazo[1,2-b]pyridazin-8-yl]-N-methyl-carba mate (18G)
[00379] [(1R)-2-[(3-fluoro-4-methoxy-5-nitro-phenyl)methoxy]-1-methy l-ethyl]ammonium;2,2,2- trifluoroacetate (18F)(0.6 g, 1.6 mmol) was dissolved in DMF (10 mL),HATU (0.91g, 2.41mmol), DIPEA(0.41g, 3.2 mmol) and intermediate 1(0.52 g, 1.6 mmol) were added to the solution in room temperature. After 18h, the solution mixture was diluted with EA (50 mL), washed with water (2×50 mL) and brine (50 mL), dried with Na 2 SO 4 and concentrated. The crude product was purified by flash chromatography to afford the title compound (18G) (545 mg, 59.89%) as a white solid. LC-MS (ESI): m/z =567.2 [M+H] +
Step 7：tert-butyl N-[3-[[(1R)-2-[(3-amino-5-fluoro-4-methoxy-phenyl)methoxy]-1 -methyl- ethyl]carbamoyl]-6-chloro-imidazo[1,2-b]pyridazin-8-yl]carba mate (18H)
[00380] tert-butyl N-[6-chloro-3-[[2-[(2-fluoro-4-methoxy-5-nitro-phenyl)methox y]-1-methyl- ethyl]carbamoyl]imidazo[1,2-b]pyridazin-8-yl]carbamate(18G) (545 mg, 0.96 mmol) was dissolved in ethanol (45 mL) and H 2 O (15 mL), iron powder (540 mg,9.62 mmol) and NH 4 Cl(310 mg,5.77 mmol) were added to solution, then the reaction mixture heated to 85 °C for 3 h, After cooling to room temperature, reaction filtered, filtrate was removed in vacuo, The residue was purified by flash chromatography (PE/EA = 2:1) to afford the title compound (18H)(450 mg, 87.2%) as a white solid. LC- MS (ESI): m/z =537.1 [M+H] +
Step 8：tert-butyl ((7R,E)-3 5 -fluoro-3 6 -methoxy-7-methyl-9-oxo-5-oxa-2,8-diaza-1(6,3)- imidazo[1,2-b]pyridazina-3(1,3)-benzenacyclononaphane-1 8 -yl)(methyl)carbamate(18I) [00381] To a solution of (18H) (450 mg, 0.84 mmol) in 1,4-dioxane (100 mL) were added Cs 2 CO 3 (820 mg, 2.51 mmol) and 3rd-t-Bu-Xphos-Pd (250 mg). The reaction mixture was stirred at 80°C for 2 h under N 2 . After cooled to room temperature, the solvent was removed, and the residue was purified by silica gel flash column chromatography to afford the product (18I) (220 mg, 52.50%) as a white solid. LC-MS (ESI): m/z =501.3 [M+H] + .
Step 9：(7R,E)-3 5 -fluoro-3 6 -methoxy-7-methyl-1 8 -(methylamino)-5-oxa-2,8-diaza-1(6, 3)-imidazo[1, 2-b]pyridazina-3(1, 3)-benzenacyclononaphan-9-one (18)
[00382] A solution of (18I) (220 mg, 0.44 mmol) and trifluoroacetic acid (1 mL) in DCM (5 mL) was stirred at room temperature for 2 h. Solvent was evaporated, and the crude product was partitioned between water and DCM. The aqueous layer was basified with NaHCO 3 and extracted with DCM.
Combined organic layers were washed with brine, dried over sodium sulfate, filtered, and evaporated, the residue was purified by silica gel flash column chromatography to afford the product 18 (71 mg, 40.34%) as a white solid. 1 H NMR (400 MHz, CDCl 3 ) d 8.76 (d, 1H), 8.20 (s, 1H), 8.07 (s, 1H), 6.87 (s, 1H), 6.63 – 6.58 (m, 1H), 6.40 (s, 1H), 5.63 (s, 1H), 4.67 (d, 1H), 4.38 (d, 1H), 4.30– 4.20 (m, 1H), 4.05 (d, 3H), 3.60– 3.56 (m, 1H), 3.48– 3.38 (m, 1H), 3.06 (d, 3H), 1.29 (d, 3H). LC-MS (ESI): m/z =401.2 [M+H] + . Example 19
Step 1：2,3-difluoro-4-methoxy-5-nitro-benzaldehyde (19B)
[00383] 2, 3-difluoro-4-methoxy-benzaldehyde (19A) (3 g,17.43 mmol ) was dissolved in concentrated sulfuric acid (18 mL) and cooled to -10°C. Concentrated nitric acid (1.5 mL) in concentrated sulfur acid (3 mL) was added dropwise over 10 min. After an additional hour of stirring at below -10°C, the mixture was poured into crushed ice. the precipitate was collected by filtration and partitioned between dichloromethane (30 mL) and saturated sodium hydrogen carbonate (30 mL). The organic layer was dried (Na 2 SO 4 ) and evaporated in vacuo to give the title compound (19B) (3.1g, 82.01%) as a white solid. LC-MS (ESI): m/z =218.1 [M+H] +
Step 2：(2,3-difluoro-4-methoxy-5-nitro-phenyl)methanol (19C)
[00384] Sodium borohydride (1.08 g,28.56 mmol) was added portionwise to a stirring solution of 2,3- difluoro-4-methoxy-5-nitro-benzaldehyde (19B) (3.1g,14.28 mmol) in methanol (60 mL) at 0°C. After 2 hours, the methanol was removed in vacuo. The residue was treated with cold water and extracted with dichloromethane. The combined organic layer was washed with brine, dried (Na 2 SO 4 ) and then evaporated in vacuo to give the title compound (19C) as a little yellow solid(2.8 g, 89.51%). LC-MS (ESI): m/z = 220.1 [M+H] +
Step 3：1-(bromomethyl)-2,3-difluoro-4-methoxy-5-nitro-benzene (19D)
[00385] Carbon tetrabromide (8.47 g,25.56 mmol) in anhydrous diethyl ether (30 mL) was added dropwise to a stirred solution of (2,3-difluoro-4-methoxy-5-nitro-phenyl)methanol (19C) (2.8 g,12.78 mmol) and triphephosphine (6.7 g,25.56 mmol) in anhydrous diethyl ether (100 mL). The mixture was stirred overnight before it was concentrated down to a sticky oil. Silica gel chromatography with ethyl acetate in hexane (0-10%) gave the title compound (19D) as a pale yellow solid (2.12 g, 59.05%). LC- MS (ESI): m/z =281.9 [M+H] +
Step 4：tert-butyl N-[(1R)-2-[(2,3-difluoro-4-methoxy-5-nitro-phenyl)methoxy]-1 -methyl- ethyl]carbamate (19E)
[00386] Sodium hydride (92 mg, 2.31mmol) was added portionwise to a stirred solution of tert-butyl N- [(1R)-2-hydroxy-1-methyl-ethyl]carbamate (0.405 g, 2.31 mmol) in THF (15 mL) at 0°C, the mixture was stirred at 0°C for 10 min. then 1-(bromomethyl)-2,3-difluoro-4-methoxy-5-nitro-benzene (19D) (0.65 g, 2.31 mmol) was added to the mixture at 0°C, after 10 min, The mixture was treated with cold water and extracted with ethyl acetate. The combined organic layer was washed with brine, dried (Na 2 SO 4 ) and then the residue was purified by flash chromatography to afford the title compound (19E)(0.3 g, 34.48%) as a brown solid. LC-MS (ESI): m/z =377.1 [M +H] +
Step 5：[(1R)-2-[(2,3-difluoro-4-methoxy-5-nitro-phenyl)methoxy] -1-methyl- ethyl]ammonium;2,2,2-trifluoroacetate (19F)
[00387] Trifluoroacetic acid (1.5 mL) was added to a solution of tert-butyl N-[(1R)-2-[(2,3-difluoro-4- methoxy-5-nitro-phenyl)methoxy]-1-methyl-ethyl] carbamate (19E)(0.3 g, 0.8 mmol) in DCM(5 mL), The mixture was stirred 2 h, the mixture solution was evaporated to dryness, then the title
compound(19F) (0.28 g.90.03%) was obtained as brown liquid, which was used in the next step without further purification. LC-MS (ESI): m/z =277.2 [M+H] +
Step 6：tert-butyl N-[6-chloro-3-[[(1R)-2-[(2,3-difluoro-4-methoxy-5-nitro-phen yl)methoxy]-1- methyl-ethyl]carbamoyl]imidazo[1,2-b]pyridazin-8-yl]carbamat e (19G)
[00388] [(1R)-2-[(2,3-difluoro-4-methoxy-5-nitro-phenyl)methoxy]-1-m ethyl-ethyl]ammonium;2,2,2- trifluoroacetate(19F)(0.28 g, 0.71 mmol) was dissolved in DMF (5 mL), HATU (0.41 g, 1.07 mmol), DIPEA(0.185 g, 1.43 mmol) and intermediate 1(0.24 g, 0.71 mmol) were added to the solution in room temperature. After 18 h, the solution mixture was diluted with EA (50 mL), washed with water (2×50 mL) and brine (50 mL), dried with Na 2 SO 4 and concentrated. The crude product was purified by flash chromatography (PE/EA = 2:1) to afford the title compound (19G) (0.18 g, 42.95%) as a white solid. LC- MS (ESI): m/z =585.2 [M+H] +
Step 7：tert-butyl N-[3-[[(1R)-2-[(5-amino-2,3-difluoro-4-methoxy-phenyl)methox y]-1-methyl- ethyl]carbamoyl]-6-chloro-imidazo[1,2-b]pyridazin-8-yl]carba mate (19H)
[00389] tert-butylN-[6-chloro-3-[[(1R)-2-[(2,3-difluoro-4-methoxy-5- nitro-phenyl)methoxy]-1-methyl- ethyl]carbamoyl]imidazo[1,2-b]pyridazin-8-yl]carbamate (19G) (0.18 g, 0.3 mmol) was dissolved in ethanol (30 mL) and H 2 O (10 mL), Fe powder (172 mg,3.08 mmol) and NH 4 Cl (100 mg,1.85mmol) were added to solution, then the reaction mixture heated to 85 °C for 3 h, After cooling to room temperature, reaction filtered, filtrate was removed in vacuo, The residue was purified by flash chromatography to afford the title compound (19H) (80 mg, 47.05%) as a white solid. LC-MS (ESI): m/z =555.2 [M+H] + Step 8：tert-butyl ((7R,E)-3 4 ,3 5 -difluoro-36-methoxy-7-methyl-9-oxo-5-oxa-2,8-diaza-1( 6,3)- imidazo[1,2-b]pyridazina-3(1,3)-benzenacyclononaphane-1 8 -yl)(methyl)carbamate (19I)
[00390] To a solution of (19H) (80 mg, 0.14 mmol) in 1,4-dioxane (40 mL) were added Cs 2 CO 3 (141 mg, 0.43 mmol) and 3rd-t-Bu-Xphos-Pd (50 mg). The reaction mixture was stirred at 80°C for 2 h under N2. After cooled to room temperature, the solvent was removed, and the residue was purified by silica gel flash column chromatography to afford the product (19I) (42 mg, 47.05%) as a white solid. LC-MS (ESI): m/z =519.3 [M+H] +
Step 9：(7R,E)-3 4 ,3 5 -difluoro-3 6 -methoxy-7-methyl-1 8 -(methylamino)-5-oxa-2,8-diaza-1(6,3)- imidazo[1,2-b]pyridazina-3(1,3)-benzenacyclononaphan-9-one
[00391] A solution of 19I (42mg, 0.081mmol) and trifluoroacetic acid (0.3 mL) in DCM (4 mL) was stirred at room temperature for 2 h. Solvent was evaporated, and the crude product was partitioned between water and DCM. The aqueous layer was basified with NaHCO 3 and extracted with DCM.
Combined organic layers were washed with brine, dried over sodium sulfate, filtered, and evaporated, the residue was purified by silica gel flash column chromatography to afford the product 19 (12 mg, 36.36%) as a white solid. 1 H NMR (400 MHz, CDCl 3 ) d 8.67 (d, 1H), 8.14 (dd, 1H), 8.08 (s, 1H), 6.72 (s, 1H), 6.58 (s, 1H), 5.63 (s, 1H), 4.71–4.60 (m, 2H), 4.31–4.21 (m, 1H), 4.10 (d, 3H), 3.61 (dd, 1H), 3.46– 3.39 (m, 1H), 3.06 (d, 3H), 1.31 (d, 3H). LC-MS (ESI): m/z =419.2[M+H] + .
Example 20
Step 1：tert-butyl N-[1-(hydroxymethyl)cyclopropyl]carbamate (20B)
[00392] Triethylamine (4.93 g,48.76 mmol) was added dropwise to a stirred solution of (1- aminocyclopropyl)methanol hydrochloride(20A) (2 g,16.25 mmol) in THF (50 mL) at 0°C. After 10 min of stirring, di-tert-butyl dicarbonate (7.09 g,32.50mmol) in THF (5 mL) was added dropwise to the mixture at 0°C. The mixture was stirred overnight in room temperature, the solvent was removed in vacuo. The residue was diluted with ethyl acetate (60 mL), washed with water (2×60 mL) and brine (50 mL), dried with Na 2 SO 4 and concentrated. then the title compound (20B) (3g.100%) was obtained as white solid, which was used in the next step without further purification. 1 H NMR (400 MHz, CDCl 3 ) d 5.05 (s, 1H), 3.59 (s, 2H), 2.40 (s, 1H), 1.44 (s, 9H), 0.83 (m, 4H).
Step 2：tert-butyl N-[1-[(4-methoxy-3-nitro-phenyl)methoxymethyl]cyclopropyl]ca rbamate (20C)
[00393] Sodium hydride (480 mg, 12.02 mmol) was added portionwise to a stirred solution of tert-butyl N-[1-(hydroxymethyl)cyclopropyl]carbamate (20B)(1.5 g,8.01 mmol) in THF (60 mL) at 0°C, the mixture was stirred at 0°C for 30 min. then 4-(bromomethyl)-1-methoxy-2-nitrobenzene (1.96 g,8.01 mmol) was added to the mixture at 0°C, The mixture was stirred overnight in room temperature, the mixture was treated with cold water (80 mL) and extracted with ethyl acetate( 2×100 mL). The combined organic layer was washed with brine, dried with Na 2 SO 4 and concentrated, then the residue was purified by flash chromatography to afford the title compound (20C)(2.4 g,85.40%) as a little yellow solid. 1 H NMR (400 MHz, CDCl 3 ) d 7.76 (d, 1H), 7.46 (dd, 1H), 7.02 (d, 1H), 5.13 (s, 1H), 4.46 (s, 2H), 3.89 (s, 3H), 3.44 (s, 2H), 1.34 (s, 9H), 0.81– 0.66 (m, 4H).
Step 3：[1-[(4-methoxy-3-nitro-phenyl)methoxymethyl]cyclopropyl] ammonium;2,2,2- trifluoroacetate (20D) [00394] Trifluoroacetic acid (1.5 mL) was added to a solution of tert-butyl N-[1-[(4-methoxy-3-nitro- phenyl)methoxymethyl]cyclopropyl]carbamate(20C) (0.55 g, 1.56 mmol) in DCM(5 mL), The mixture was stirred overnight, The mixture solution was evaporated to dryness, then the title compound(20D) (0.54 g.94.57%) was obtained as brown liquid, which was used in the next step without further purification. LC-MS (ESI): m/z =253.2[M+H] +
Step 4：tert-butyl N-[6-chloro-3-[[1-[(4-methoxy-3-nitro- phenyl)methoxymethyl]cyclopropyl]carbamoyl]imidazo[1,2-b]pyr idazin-8-yl]-N-methyl-carbamate (20E)
[00395] [1-[(4-methoxy-3-nitro-phenyl)methoxymethyl]cyclopropyl]ammo nium;2,2,2- trifluoroacetate(20D)(0.54g, 1.47 mmol) was dissolved in DMF(10 mL),HATU(0.84 g, 2.21 mmol), DIPEA(0.38 g, 2.95 mmol) and intermediate 1(0.1g, 1.47 mmol)were added to the solution in room temperature. After 18h, the solution mixture was diluted with EA (50 mL), washed with water (2×50 mL) and brine (50 mL), dried with Na 2 SO 4 and concentrated. The crude product was purified by flash chromatography to afford the title compound (20E) (0.52 g, 62.95%) as a pale solid. LC-MS (ESI): m/z =561.3[M+H] +
Step 5：tert-butyl N-[3-[[1-[(3-amino-4-methoxy-phenyl)methoxymethyl]cyclopropy l]carbamoyl]- 6-chloro-imidazo[1,2-b]pyridazin-8-yl]-N-methyl-carbamate (20F)
[00396] tert-butyl N-[6-chloro-3-[[1-[(4-methoxy-3-nitro
phenyl)methoxymethyl]cyclopropyl]carbamoyl]imidazo[1,2-b] pyridazin-8-yl]-N-methyl-carbamate (20E) (0.52 g, 0.93 mmol) was dissolved in ethanol (60 mL) and H 2 O (15 mL), iron powder (520 mg,9.28 mmol) and NH 4 Cl (0.3 g, 5.57mmol) were added to solution, then the reaction mixture heated to 85 °C for 3 h, After cooling to room temperature, reaction filtered, filtrate was removed in vacuo. The residue was purified by flash chromatography (PE/EA = 2:1) to afford the title compound (20F)(0.32 g, 65.04%) as a white solid. LC-MS (ESI): m/z =531.3[M+H] +
Step 6：tert-butyl (E)-(6'-methoxy-9'-oxospiro[cyclopropane-1,7'-5-oxa-2,8-diaz a-1(6,3)- imidazo[1,2-b]pyridazina-3(1,3)-benzenacyclononaphan]-8'-yl) (methyl)carbamate (20G)
[00397] To a solution of (20F) (320 mg, 0.6 mmol) in 1,4-dioxane (40 mL) were added Cs 2 CO 3 (590 mg, 1.81 mmol) and 3rd-t-Bu-Xphos-Pd (180 mg). The reaction mixture was stirred at 85°C for 2 h under N2. After cooled to room temperature, the solvent was removed, and the residue was purified by silica gel flash column chromatography to afford the product (20G) (175mg, 58.72%) as a white solid. LC-MS (ESI): m/z =495.3[M+H] +
Step 7：(E)-6'-methoxy-8'-(methylamino)spiro[cyclopropane-1,7'-5 -oxa-2,8-diaza-1(6,3)- imidazo[1,2-b]pyridazina-3(1,3)-benzenacyclononaphan]-9'-one (20H)
[00398] A solution of 20G (175mg, 0.35 mmol) and p-Toluenesulfonic acid monohydrate (101 mg, 0.53 mmol) in DCM (4 mL) was stirred at room temperature for 2h. Solvent was evaporated, and the crude product was partitioned between water and DCM. The aqueous layer was basified with NaHCO 3 and extracted with DCM. Combined organic layers were washed with brine, dried over sodium sulfate, filtered, and evaporated, the residue was purified by silica gel flash column chromatography to afford the product 20 (35 mg, 25.17%) as a white solid. 1 H NMR (400 MHz, CDCl 3 ) d 8.99 (s, 1H), 8.36 (d, 1H), 7.97 (s, 1H), 6.90– 6.73 (m, 3H), 6.34 (s, 1H), 5.63 (s, 1H), 4.60 (s, 2H), 3.93 (s, 3H), 3.49 (s, 2H), 3.04 (d, 3H), 1.82 (q, 2H), 0.68 (q, 2H). LC-MS (ESI): m/z =395.2[M+H] +
Example 21:
Step 1: tert-butyl (4-(5-methoxy-6-nitro-1H-indazol-1-yl)butan-2-yl)carbamate
[00399] A solution of 5-methoxy-6-nitro-1H-indazole (1.0 g, 5.18 mmol), tert-butyl (4-bromobutan-2- yl)carbamate (1.7 g, 6.73 mmol) and K 2 CO 3 (1.4 g, 10.36 mmol) in DMF (30 mL) stirred at 60 o C overnight. After reaction completed, the reaction was cooled in an ice bath and was diluted with EA (100 mL) and the solution was extracted with water (3 x 20 mL). The combined organic layers were dried over Na 2 SO 4 , filtered and concentrated under reduced pressure to afford the crude product which was purified by column chromatography (PE/EtOAc=3/1) to give the desired product (0.8 g, 42%) as a white solid. LM-MS: m/z =365.4[M+H] +
Step 2: 4-(5-methoxy-6-nitro-1H-indazol-1-yl)butan-2-amine
[00400] A solution of tert-butyl (4-(5-methoxy-6-nitro-1H-indazol-1-yl)butan-2-yl)
carbamate (0.7 g, 1.92 mmol) and trifluoroacetic acid (0.5 mL) in DCM (4 mL) was stirred at room temperature for 2h. Solvent was evaporated, and the crude product was partitioned between water and DCM. The aqueous layer was quenched with NaHCO 3 and extracted with DCM. Combined organic layers was washed with brine, dried over sodium sulfate, filtered, and evaporated to give the crude product which was purified by column chromatography (PE/EtOAc=3/1) to give the desired product (0.4 g, 79%) as a white solid. LM-MS: m/z =265.3[M+H] +
Step 3: tert-butyl (6-chloro-3-((4-(5-methoxy-6-nitro-1H-indazol-1-yl)butan-2-y l)
carbamoyl)imidazo[1,2-b]pyridazin-8-yl)(methyl)carbamate
[00401] 4-(5-methoxy-6-nitro-1H-indazol-1-yl)butan-2-amine (0.2 g, 0.76 mmol) was dissolved in DCM (5 mL), HATU (0.58 g, 1.515 mmol), DIPEA (0.15 g, 1.14 mmol) and 8-((tert- butoxycarbonyl)(methyl)amino)-6-chloroimidazo[1,2-b]pyridazi ne-3
-carboxylic acid (0.25 g, 0.76 mmol) were added to the solution in room temperature. The mixture was stirred at r.t. for 1 h, then diluted with EA (20 mL), washed with water (10 mL) and brine (10 mL), dried with Na 2 SO 4 and concentrated. The crude product was purified by flash chromatography (PE/EtOAc=3/1) to afford the title compound (0.3 g, 69%) as a white solid. LM-MS: m/z =574.0[M+H] + Step 4: tert-butyl (3-((4-(6-amino-5-methoxy-1H-indazol-1-yl)butan-2-yl)carbamo yl)
-6-chloroimidazo[1,2-b]pyridazin-8-yl)(methyl)carbamate
The tert-butyl (6-chloro-3-((4-(5-methoxy-6-nitro-1H-indazol-1-yl)butan-2-y l)
[00402] carbamoyl)imidazo[1,2-b]pyridazin-8-yl)(methyl)carbamate (0.3 g, 0.52 mmol) and NH 4 Cl (330 mg, 6.24 mmol) was dissolved in MeOH (5 mL) at r.t., Zn powder ( 405 mg, 6.24 mmol) were added to the solution, then the reaction mixture was stirred at room temperature for 1 h. After reaction completed, reaction filtered, filtrate was removed in vacuo. The residue was purified by flash chromatography to afford the title compound (0.27 g, 96 %) as a white solid. LM-MS: m/z
=543.0[M+H] +
Step 5: tert-butyl (E)-(3 5 -methoxy-6-methyl-8-oxo-3 1 H-2,7-diaza-1(6,3)
-imidazo[1,2-b]pyridazina-3(6,1)-indazolacyclooctaphane-1 8 -yl)(methyl)carbamate
To a solution of tert-butyl (3-((4-(6-amino-5-methoxy-1H-indazol-1-yl)
[00403] butan-2-yl)carbamoyl)-6-chloroimidazo[1,2-b]pyridazin-8-yl)( methyl)carbamate (270 mg, 0.5 mmol) in 1,4-dioxane (20 mL) was added Cs 2 CO 3 (325 mg, 1.0 mmol) and 3rd-t-Bu-Xphos-Pd (120 mg). The reaction mixture was stirred at 80°C for 3 h under N2. After cooled to room temperature, reaction filtered, the solvent was removed, and the residue was purified by silica gel flash column
chromatography to afford the desired compound (150 mg, 59%) as a white solid. LM-MS: m/z
=507.7[M+H] +
Step6: (E)-3 5 -methoxy-6-methyl-1 8 -(methylamino)-3 1 H-2,7-diaza-1(6,3)-imidazo
[1,2-b]pyridazina-3(6,1)-indazolacyclooctaphan-8-one
A solution of tert-butyl (E)-(3 5 -methoxy-6-methyl-8-oxo-3 1 H-2,7-diaza-1(6,3)
[00404] -imidazo[1,2-b]pyridazina-3(6,1)-indazolacyclooctaphane-1 8 -yl)(methyl)carbamate (0.15 g, 0.3 mmol) and trifluoroacetic acid (0.2 mL) in DCM (4 mL) was stirred at room temperature for 2 h. Solvent was evaporated, and the crude product was partitioned between water and DCM. The aqueous layer was quenched by NaHCO 3 and extracted with DCM. Combined organic layers were washed with brine, dried over sodium sulfate, filtered. The filtrate was purified directly by prep-HPLC to give the desired product 21 (125 mg, yield: 42%) as a brow solid. LM-MS: m/z =407.5[M+H] + . 1 H NMR (400 MHz, CD 3 OD) d 8.88 (s, 1H), 7.93 (s, 1H), 7.85 (s, 1H), 7.22 (s, 1H), 6.19 (s, 1H), 4.61– 4.48 (m, 3H), 4.00 (s, 3H), 3.04 (s, 3H), 2.14 (d, 2H), 1.01 (d, 3H).
Example 22
Step 1：tert-butyl ((1s,3s)-3-hydroxycyclobutyl)carbamate (22B)
[00405] Triethylamine (4.93g, 48.76 mmol) was added dropwise to a stirred solution of (1s,3s)-3- hydroxycyclobutan-1-aminium chloride (22A) (2g, 16.25mmol) in THF (50 mL) at 0°C. After 10 min of stirring, ditertbutyl dicarbonate (7.09g, 32.50mmol) in THF (5 mL) was added dropwise to the mixture at 0°C. The mixture was stirred overnight in room temperature, the solvent was removed in vacuo. The residue was diluted with ethyl acetate (60 mL), washed with water (2×60 mL) and brine (50 mL), dried with Na 2 SO 4 and concentrated. then the title compound (22B) (3g.100%) was obtained as colorless oil, which was used in the next step without further purification. LC-MS (ESI): m/z =188.1 [M+H] +
Step 2：tert-butyl ((1s,3s)-3-((4-methoxy-3-nitrobenzyl)oxy)cyclobutyl)carbamat e (22C)
To a stirred solution of tert-butyl ((1s,3s)-3-hydroxycyclobutyl)carbamate (22B) (1.5g,8.02mmol) in THF (60 mL) was added sodium hydride (577mg, 14.43mmol) portionwise at 0°C, the mixture was stirred at 0°C for 30 min. Then 4-(bromomethyl)-1-methoxy-2-nitrobenzene (0.69g, 2.62mmol) was added to the mixture at 0°C, The mixture was stirred overnight in room temperature, the mixture was treated with cold water (80 mL) and extracted with ethyl acetate( 2×100 mL). The combined organic layer was washed with brine, dried with Na2SO4 and concentrated, then the residue was purified by flash chromatography to afford the title compound (22C) (0.2 g, 21.30%) as a white solid. LC-MS (ESI): m/z =353.1 [M+H] + Step 3：(1s,3s)-3-((4-methoxy-3-nitrobenzyl)oxy)cyclobutan-1-ami nium chloride (22D)
To a solution of tert-butyl ((1s,3s)-3-((4-methoxy-3-nitrobenzyl)oxy) cyclobutyl)carbamate (222C) (0.55g, 2.79mmol) in DCM (5 mL) was added trifluoroacetic acid (1.5 mL), The mixture was stirred overnight at r.t., The mixture solution was evaporated to dryness, then the title compound (22D) (0.39g. 100%) was obtained as white solid, which was used in the next step without further purification. LC-MS (ESI): m/z =253.1 [M+H] +
Step 4：tert-butyl tert-butyl (6-chloro-3-(((1s,3s)-3-((4-methoxy-3-nitrobenzyl)
oxy)cyclobutyl)carbamoyl)imidazo[1,2-b]pyridazin-8-yl)(me thyl)carbamate (22E)
[00406] (1s,3s)-3-((4-methoxy-3-nitrobenzyl)oxy)cyclobutan-1-aminium chloride (22D) (200 mg, 0.79 mmol) was dissolved in DMF(5 mL), HATU(360 mg, 0.94 mmol), DIPEA(180mg, 1.18mmol) and intermediate 1(257mg, 0.79mmol)were added to the solution in room temperature. The mixture was stirred at r.t. for 18h, then diluted with EA (50 mL), washed with water (2×50 mL) and brine (50 mL), dried with Na 2 SO 4 and concentrated. The crude product was purified by flash chromatography (PE/EA = 2:1) to afford the title compound (22E) (400 mg, 90.00%) as a white solid. LC-MS (ESI): m/z =561.2 [M+H] +
Step 5：tert-butyl (6-chloro-3-(((1s,3s)-3-((4-methoxy-3-nitrobenzyl)oxy)cyclob utyl)
carbamoyl)imidazo[1,2-b]pyridazin-8-yl)(methyl)carbamate (22F)
[00407] (6-chloro-3-(((1s,3s)-3-((4-methoxy-3-nitrobenzyl)oxy)cyclob utyl)carbamoyl) imidazo[1,2- b]pyridazin-8-yl)(methyl)carbamate (22E) (280 mg, 0.5 mmol) was dissolved in ethanol (9 mL) and H 2 O (3 mL), iron powder (560 mg,10 mmol) and NH 4 Cl (530 mg,10 mmol) were added to solution, then the reaction mixture heated to 85 °C for 3 h, After cooling to room temperature, reaction filtered, filtrate was removed in vacuo. The residue was purified by flash chromatography to afford the title compound (22H) (200 mg, 75.47%) as a white solid. LC-MS (ESI): m/z =531.2 [M+H] + .
Step 6：tert-butyl ((6 1 s,6 3 s,E)-3 6 -methoxy-8-oxo-5-oxa-2,7-diaza-1(6,3)-imidazo [1,2-b]pyridazina- 3(1,3)-benzena-6(1,3)-cyclobutanacyclooctaphane-18-yl)(methy l) carbamate (22G)
[00408] To a solution of (6-chloro-3-(((1s,3s)-3-((4-methoxy-3-nitrobenzyl)oxy)cyclob utyl) carbamoyl)imidazo[1,2-b]pyridazin-8-yl)(methyl)carbamate (22F) (265 mg, 0.5 mmol) in 1,4-dioxane (10 mL) were added Cs 2 CO 3 (326 mg, 1.0 mmol) and 3rd-t-Bu-Xphos-Pd (35 mg, 0.04 mmol). The reaction mixture was stirred at 80°C for 3 h under N2. After cooled to room temperature, the solvent was removed, and the residue was purified by silica gel flash column chromatography to afford the product Compound (22G) (50mg, 20.24%) as a white solid. LC-MS (ESI): m/z =495.1 [M+H] +
Step 7：6 1 s,6 3 s,E)-36-methoxy-1 8 -(methylamino)-5-oxa-2,7-diaza-1(6,3)-imidazo[1,2-b]py ridazina- 3(1,3)-benzena-6(1,3)-cyclobutanacyclooctaphan-8-one (22)
[00409] A solution of tert-butyl tert-butyl ((6 1 s,6 3 s,E)-3 6 -methoxy-8-oxo-5-oxa- 2,7-diaza-1(6,3)- imidazo[1,2-b]pyridazina-3(1,3)-benzena-6(1,3)-cyclobutanacy clooctaphane-18-yl)(methyl) carbamate (22G) (50mg, 1.02 mmol) and p-Toluenesulfonic acid monohydrate (50mg, 0.29mol) in DCM (4 mL) was stirred at room temperature for 2h. Solvent was evaporated, and the crude product was partitioned between water and DCM. The aqueous layer was basified with NaHCO 3 and extracted with DCM.
Combined organic layers and evaporated, the residue was purified by TLC to afford 22 (2 mg, 5.01%). LC-MS (ESI): m/z =395.1 [M+H] + . 1 H NMR (400 MHz, DMSO-d6) d 9.15 (d, 1H), 8.29 (s, 1H), 8.18 (d, 1H), 7.82 (s, 1H), 7.38 (d, 1H), 7.00 (d, 1H), 6.85 (dd, 1H), 6.23 (d, 1H), 4.50 (s, 2H), 4.34 (dd, 1H), 4.21 (s, 1H), 3.88 (s, 3H), 2.89 (d, 3H), 2.72– 2.65 (m, 2H), 1.75 (d, 2H).
Example 24:
Step 1: Example 24b
[00410] To a solution of Example 24a (10.0 g, 60.24 mmol, 1.0 eq) in AcOH (150 mL) was added a solution of NaNO 2 (4.99 g, 72.29 mmol, 1.2 eq) in H 2 O (10 mL) dropwise at r.t. The reaction mixture was stirred for 2 h at r.t. Then, water (100 mL) was added to the mixture, which was stirred for 30 min. The precipitated solid was collected by filtration, which was washed with H 2 O and MTBE. The crude product was purified by silica gel flash column chromatography to afford the desired product Example 24b (3.9 g, 36.6% yield) as a yellow solid. LCMS [M+1] + = 178.2.
Step 2: Example 24c
[00411] To a solution of Example 24b (3.9 g, 22.03 mmol, 1.0 eq) in CH3CN (50 mL) was added CH3I (15.6 g, 110.17 mmol, 5.0 eq) and K 2 CO 3 (7.6 g, 55.08 mmol, 2.5 eq). The reaction mixture was stirred for 16 h at 80 o C. The solvent was concentrated, and the crude was purified by silica gel flash column chromatography to afford the desired product Example 24c (3.17 g, 75.3% yield) as a brown solid and its isomer (200 mg) as a brown solid. LCMS [M+1] + = 192.2. Example 24c: 1 H NMR (300 MHz, DMSO- d6) d 8.21 (d, 1H), 8.05 (s,1H), 7.79 (s,1H), 4.33 (s, 3H), 2.51 (s, 3H). Isomer: 1 H NMR (300 MHz, DMSO-d6) d 8.04 (s,1H), 7.96 (s, 1H), 7.79 (s,1H), 4.23 (s, 3H), 2.51 (s, 3H).
Step 3: Example 24d
[ 00412] A solution of Example 24c (500 mg, 2.62 mmol, 1.0 eq) in CCl 4 (12 mL) was heated to 80 o C, to which NBS (559 mg, 3.14 mmol, 1.2 eq) and AIBN (429 mg, 2.62 mmol, 1.0 eq) were added. The reaction mixture was stirred for 4 h at 80 o C. After cooling to room temperature, the reaction mixture was concentrated, and the crude was purified by silica gel flash column chromatography to afford the desired product Example 24d (577 mg, 81.7% yield) as a yellow solid. 1 H NMR (300 MHz, DMSO-d 6 ) d 8.78 (s, 1H), 8.39 (d, 1H), 8.36 (d, 1H), 4.95 (s, 2H), 4.28 (s, 3H).
Step 4: Example 24f
[00413] To a solution of Example 24e (681 mg, 3.89 mmol, 2.0 eq) in THF (10 mL) was added NaH (117 mg, 60% in mineral oil, 2.92 mmol, 1.5 eq) at 0 o C. After stirring for 30 min, Example 24d (525 mg, 1.94 mmol, 1.0 eq) was added to the mixture, which was stirred for another 2 h at r.t. The mixture was quenched with saturated aqueous NH 4 Cl and extracted with EtOAc (30 mL*3). The combined organic layers were washed with brine, dried over Na 2 SO 4 and concentrated. The residue was purified by silica gel flash column chromatography to afford the desired product Example 24f (306 mg, 43.2% yield) as a yellow solid. LCMS [M+1-56] + = 309.2.
Step 5: Example 24g
[00414] Example24f (306 mg, 0.84 mmol, 1.0 eq) was dissolved in MeOH (10 mL), and 10% Pd/C (200 mg) was added slowly in portions under N 2 protection. The system was evacuated and then refilled with hydrogen. The mixture solution was stirred for 1 h at r.t. under H 2 balloon. The reaction mixture was filtered through a Celite pad and the filtrate was concentrated. The residue was purified by prep-TLC to afford the desired product Example 24g (150 mg, 53.3% yield) as a light yellow solid. LCMS
[M+1+22] + = 357.3.
Step 6: Example 24h
[00415] To a solution of Example 24g (130 mg, 0.39 mmol, 1.0 eq) in DCM (6 mL) was added HCl/dioxane (2 mL, 4 M in dioxane). The reaction solution was stirred for 0.5 h at r.t. and concentrated to afford the desired product Example 24h (240 mg, crude) as a yellow solid. LCMS [M+1] + = 235.3. Step 7: Example 24j
[00416] To a solution of Example 24i (173 mg, 0.53 mmol, 1.0 eq) in DCM (10 mL) were added DIEA (545 mg, 4.23 mmol, 8.0 eq) and HATU (221 mg, 0.58 mmol, 1.1 eq). After stirring for 30 min,
Example 24h (215 mg, 0.79 mmol, 1.5 eq) was added. The reaction mixture was stirred for 2 h at r.t. The solvent was removed, and the crude was purified by prep-TLC (EtOAc) to afford the desired product example 24j (250 mg, 87.0ield) as yellow oil. LCMS [M+1] + = 543.3.
Step 8: Example 24k
[00417] To a solution of Example 24j (150 mg, 0.28 mmol, 1.0 eq) in dioxane (20 mL) were added Cs 2 CO 3 (180 mg, 0.55 mmol, 2.0 eq) and 3 rd -t-Bu-Xphos-Pd (25 mg, 0.028 mmol, 0.1 eq). The reaction mixture was stirred for 16 h at 80 o C under N2. The reaction mixture was concentrated and purified by prep-TLC to afford the desired product Example 24k (50 mg, 35.7% yield) as a yellow solid. LCMS [M+1] + = 507.3.
Step 9: Example 24
[00418] To a solution of Example 24k (45 mg, 0.089 mmol, 1.0 eq) in DCM (3 mL) was added HCl/dioxane (1 mL, 4 M in dioxane). The reaction solution was stirred for 1 h at r.t., and the solvent was concentrated. The crude product was dissolved in MeOH and Na 2 CO 3 was added. The mixture was stirred for 10 min at r.t. and then filtered. The filtrate was concentrated and the residue was purified by prep- TLC to afford the desired product Example 24 (20.0 mg, 55.4% yield) as an off-white solid. LCMS [ M+1]+ = 407.3. 1H NMR (300 MHz, DMSO-d 6 ) d 9.19 (s, 1H), 8.95 (d, 1H), 8.32 (s, 1H), 7.95 (s, 1H), 7.83 (s, 1H), 7.44 (d, 1H), 7.20 (s, 1H), 6.36 (s, 1H), 4.73 (d, 1H), 4.45 (d, 1H), 4.21 (s, 3H), 4.08-3.96 (m, 1H), 3.48 (d, 1H), 3.26-3.20(m, 1H), 2.90 (d, 3H), 1.11 (d, 3H).
Example 25:
Step 1: Example 25c
[00419] To a solution of Example 25b (2.3 g, 13.4 mmol) in THF (40 mL) was added NaH (0.9 g, 60% in mineral oil, 22.4 mmol) in portions at 0 o C. After stirring for 10 min, a solution of Example 25a (2.2 g, 8.9 mmol) in THF (10 mL) was added dropwise. The reaction mixture was stirred for 3 h at r.t. The reaction was quenched with saturated NH 4 Cl aqueous solution (20 mL) at 0 o C and extracted with EtOAc (50 mL*2). The combined organic layer was dried over Na 2 SO 4 and concentrated in vacuo. The crude product was purified by silica gel flash column chromatography to afford the desired product Example 25c (2.1 g, 69.4% yield) as a yellow solid. LCMS [M+1] + = 341.3.
Step 2: Example 25d
[00420] Example 25c (1.1 g, 3.2 mmol) was dissolved in MeOH (50 mL); 10% Pd/C (110 mg) was added in portions under N 2 protection. The system was evacuated and then refilled with hydrogen. The mixture was stirred for 1 h at r.t. under H 2 balloon, and then filtered. The filtrate was concentrated and the crude product was purified by silica gel flash column chromatography to afford the desired product Example 25d (850 mg, 85.7% yield) as colorless oil. LCMS [M+1] + = 311.3.
Step 3: Example 25e [00421] To a solution of Example 25d (840 mg, 2.7 mmol) in DCM (5 mL) and MeOH (1 mL) was added HCl/dioxane (1 mL, 4M in Dioxane, 4 mmol). The reaction mixture was stirred for 1 h at r.t. The reaction solution was concentrated in vacuo to afford the desired product Example 25e (800 mg, crude) as a white solid. LCMS [M+1] + = 211.2.
Step 4: Example 25g
[00422] To a solution of Example 25f (607 mg, 1.9 mmol) in DCM (30 mL) were added DIEA (1.92 g, 14.9 mmol) and HATU (1.06 g, 2.8 mmol). After stirring for 0.5 h, Example 25e (790 mg, 2.8 mmol) was added. The reaction mixture was stirred for 2 h at r.t. The solvent was removed and the residue was purified by silica gel flash column chromatography to afford the desired product Example 25g (310 mg, 21.4% yield) as a yellow solid. LCMS [M+1] + = 519.3.
Step 5: Example 25h
[00423] To a solution of Example 25g (300 mg, 0.6 mmol) in dioxane (50 mL) were added Cs 2 CO 3 (377 mg, 1.2 mmol) and 3rd-t-Bu-Xphos-Pd (154 mg, 0.2 mmol). The reaction mixture was stirred at 90°C for 6 h under N2. After cooling to room temperature, the solvent was removed, and the residue was purified by silica gel column chromatography to afford the product Example 25h (110 mg, 39.4% yield) as a yellow solid. LCMS [M+1] + = 483.2.
Step 6: Example 25
[00424] To a solution of Example 25h (110 mg, 0.23 mmol) in DCM (5 mL) was added HCl/dioxane (1 mL, 4M in Dioxane, 4 mmol). The reaction mixture was stirred at r.t. for 5 h and then concentrated in vacuum. The residue was dissolved in MeOH (5 mL), and basified with NaHCO 3 (pH = 8). The precipitate was filtered out, and the filtrate was concentrated. The residue was purified by Prep-TLC to afford the desired product Example 25 (57.3 mg, 65.7% yield) as an off-white solid. LCMS [M+1] + = 383.2. 1 H NMR (300 MHz, DMSO-d6) d 8.92 (s, 1H), 8.36 (d, 1H), 8.24 (d, 1H), 8.11 (s, 1H), 7.81 (d, 1H), 6.99 (d, 1H), 6.92 (d, 1H), 5.94 (s, 1H), 4.57 (d, 1H), 4.40 (d, 1H), 3.94 - 3.83 (m, 4H), 3.47 (d, 1H),3.29 - 3.25 (m, 1H), 2.92 (d, 3H), 1.14 (d, 3H).
Example 26:
Step 1: Example 26b
[ 00425] To a solution of Example 26a (30.0 g, 179.0 mmol) in CCl 4 (150 mL) were added BPO (4.4 g, 17.9 mmol), NBS (38.2 g, 216.0 mmol). The reaction mixture was stirred at 100 o C for overnight, and then diluted by DCM, washed by water, and dried over anhydrous Na 2 SO 4 . The solution was
concentrated under reduced pressure to afford crude residue Example26b (37.0 g, yield 84.4%) as a yellow solid, which was used in the next step directly without further purification. LCMS [M+1] + = 246.0. 1 H NMR (400 MHz, Chloroform-d) d 7.87 (d, 1H), 7.57 (dd, 1H), 7.07 (d, 1H), 4.46 (s, 2H), 3.96 (d, 3H).
Step 2: Example 26d
[00426] To a solution of Example 26b (2.4 g, 9.8 mmol) in THF (20 mL) was added NaH (400 mg, 60% in mineral oil, 10.0 mmol) at 0 o C. The reaction mixture was warmed to room temperature and stirred at r.t. for 0.5 h. Then Example 26c (1.7 g, 9.7 mmol) was added and the mixture was stirred at r.t. for 6 h. The reaction mixture was quenched by sat. NH 4 Cl (aq.), extracted by EtOAc, and dried over anhydrous Na 2 SO 4 . The solution was concentrated under reduced pressure. The residue was purified by silica gel column chromatography to give Example26d (3.3 g, yield: 96.8%) as a yellow solid. LCMS [M+1-100] + = 241.1.
Step 3: Example 26e
[00427] A solution of Example 26d (688 mg, 2.0 mmol) and 10% Pd/C (34 mg) in MeOH (10 mL) was stirred at r.t. for 2 h under 1 atm H 2 . The mixture was filtered, and the filtrate was concentrated under reduced pressure. The residue Example 26e (640 mg, yield: quant.) was obtained as a yellow solid which was used in the next step directly. LCMS [M-174] + = 136.1
Step 4: Example 26f [00428] To a solution of Example 26e (crude 550 mg, 1.77 mmol) in DCM (10 mL) was added TFA (2.0 mL), which was stirred at r.t. for 2 h. The mixture was concentrated, and the residue was treated with EtOAc (30 mL) to give the crude product Example 26f (340 mg, yield: quant.) as a white solid. LCMS [M-74] + = 137.1.
Step 5: Example 26h
[00429] To a solution of Example 26f (crude 300 mg, 1.42 mmol), Example 26g (464 mg, 1.42 mmol) DIPEA (916 mg, 7.1 mmol) in DCM (10 mL) was added HATU (538 mg, 1.42 mmol). The reaction mixture was stirred at r.t. for 1 h. Then EtOAc (40 mL) was added to the reaction mixture, which was washed with brine (20 mL*2), dried over anhydrous Na 2 SO 4 and concentrated. The residue was purified by silica gel column chromatography to afford the desired product Example 26h (600 mg, yield: 81.4%) as a white solid. LCMS [M+1] + = 520.2.
Step 6: Example 26i
[00430] To a mixture of Example 26h (330 mg, 0.97 mmol), Cs 2 CO 3 (652 mg, 2.0 mmol) in dioxane (10 mL) was added 3rd-t-Bu-Xphos-Pd (89 mg, 0.1 mmol). The mixture was degassed with N2 three times, and stirred for 3 h at 80°C. Then the reaction mixture diluted by DCM, washed by water, dried over anhydrous Na 2 SO 4 , and then concentrated under reduced pressure to afford crude Example 26i (400 mg, crude yield >100%) as a white solid, which was used in the next step without further purification. LCMS [M+1] + = 484.2
Step 7: Example 26
[00431] To a solution of Example 26i (400 mg, 0.82 mmol) in DCM (4 mL) was added TFA (1.0 mL), which was stirred at r.t. for 1 h. The mixture was concentrated, and the residue was purified by Prep- HPLC to afford the desired product Example 26 (18.3 mg, yield 5.8% over two steps) as a white solid. LCMS [M+1] + = 384.1. 1 H NMR (400 MHz, DMSO-d6) d 9.11 (s, 1H), 8.60-8.54 (m, 1H), 8.10 (s, 1H), 8.04 (d, 1H), 7.89 (d, 1H), 7.71 (s, 1H), 5.90 (s, 1H), 4.53 (d, 1H), 4.43 (d, 1H), 3.94 (s, 3H), 3.86 (s, 1H), 3.46 (d, 1H), 3.25 (s, 1H), 2.89 (d, 3H), 1.11 (d, 3H).
Example 27:
Step 1: Example 27b
[ 00432] To a solution of Example27a (30.0 g, 277.8 mmol, 1.0 eq), Na 2 CO 3 (20.6 g, 194.5 mmol, 0.7 eq) in H 2 O (150 mL) were added KI (59.9 g, 361.1 mmol, 1.3 eq) and I2 (56.4 g, 222.2 mmol, 0.8 eq) in H 2 O (50 mL) at 100 o C, which was stirred for 16 h. After cooling to r.t., the reaction mixture was quenched with Na 2 SO 3 (35.0 g, 277.8 mmol, 1.0 eq) and extracted with DCM (300 mL*2). The combined organic layers were washed with brine, dried over Na 2 SO 4 and concentrated. The residue was purified by silica gel flash column chromatography to afford the product Example 27b (8.4 g, 12.9% yield) as a yellow solid. LCMS [M+1] + = 235.1
Step 2: Example 27c
[00433] To a solution of Example 27b (7.0 g, 50.7 mmol, 1.0 eq) in dioxane (50 mL) was added Boc 2 O (33.2 g, 152.1 mmol, 3.0 eq), which was stirred for 16 h at 100 o C. The reaction mixture was concentrated and the residue was purified by silica gel flash column chromatography to afford the product Example 27c (3.5 g, 35% yield) as a white solid. LCMS [M+1] + = 335.1.
Step 3: Example 27d
[00434] To a solution of Example 27c (3.5 g, 10.5 mmol, 1.0 eq) and MeONa (2.82 g, 52.25 mmol, 5.0eq) in MeOH (30 mL) were added Cs 2 CO 3 (10.2 g, 21.0 mmol, 2.0 eq), CuI (199 mg, 1.05 mmol, 0.1 eq), and L-proline (343 mg, 2.1 mmol, 0.2 eq). The reaction mixture was stirred for 8 h at 60 o C under N2 protection. The reaction mixture was concentrated. The residue was purified by silica gel flash column chromatography to afford the product Example 27d (750 mg, 30.1% yield) as a white solid. LCMS [M+1] + = 239.3.
Step 4: Example 27e [00435] To a solution of Example 27d (550 mg, 2.3 mmol, 1.0 eq) in DCM (10 mL) at 0 o C (ice-water bath) was added m-CPBA (596 mg, 3.45 mmol, 1.5 eq) portionwise. After addition, the reaction was stirred for 1 h at r.t. The solution was quenched with Na 2 SO 3 (150 mg, 1.15 mmol, 0.5 eq) and extracted with DCM (30 mL*2). The combined organic layers were washed with brine, dried over Na 2 SO 4 and concentrated to afford the product Example 27e (550 mg, 93.8% yield) as a yellow solid. LCMS [M+1] + = 255.3.
Step 5: Example 27f
[00436] A solution of Example 27e (400 mg, 1.65 mmol, 1.0 eq) in Ac 2 O (10 mL) was stirred for 1 h at 100 o C. The mixture was concentrated to afford the crude product Example 27f (550 mg, quant. yield) as brown oil. LCMS [M+1] + = 297.3.
Step 6: Example 27g
[00437] To a solution of Example 27f (450 mg, 1.52 mmol, 1.0 eq) in MeOH (15 mL) and H 2 O (5 mL) was added K 2 CO 3 (418.2 mg, 3.04 mmol, 2.0 eq) at 0 o C. The mixture was stirred for 2 h at 50 o C. After the reaction was completed, the mixture was concentrated in vacuo. The residue was purified by silica gel flash column chromatography to afford the desired product Example 27g (250 mg, 64.6% yield) as a white solid. LCMS [M+1] + = 255.2.
Step 7: Example 27h
[00438] To a solution of Example 27g (230 mg, 0.90 mmol, 1.0 eq), CBr4 (597 mg, 1.80 mmol, 2.0 eq) in DCM (15 mL) was added PPh3 (355 mg, 1.35 mmol, 1.5 eq) in DCM (5 mL) at 0 o C, which was stirred for 1 h at r.t. under N2 protection. The mixture was concentrated in vacuo. The residue was purified by silica gel flash column chromatography to afford the product Example 27h (150 mg, 52.7% yield) as yellow oil. LCMS [M+1] + = 317.2.
Step 8: Example 27j
[00439] To a solution of Example 27i (165.6 mg, 0.94 mmol, 1.5 eq) in THF (5 mL) was added NaH (75.7 mg, 60% in mineral oil, 1.89 mmol, 3.0 eq) in portions at 0 o C. After stirring for 0.5 h, a solution of Example 27h (200 mg, 0.63 mmol, 1.0 eq) in THF (1 mL) was added dropwise. The reaction mixture was stirred for 1.5 h at r.t. The reaction was quenched with saturated NH 4 Cl aqueous solution (10 mL) at 0 o C and extracted with EtOAc (20 mL*3). The combined organic layers were washed with brine (10 mL*2), dried over Na 2 SO 4 and concentrated in vacuo. The crude product was purified by silica gel flash column chromatography to afford the desired product Example 27j (105 mg, 40.4% yield) as a light yellow solid. LCMS [M+1] + = 412.4.
Step 9: Example 27k
To a solution of Example 27j (105 mg, 0.25 mmol, 1.0 eq) in DCM (15 mL) was added HCl/dioxane (0.2 mL, 4 mol/L in dioxane) at 0 o C. The reaction mixture was stirred for 1 h at r.t. The reaction solution was concentrated in vacuo to afford the desired product Example 27k (150 mg, quant. yield) as a white solid. LCMS [M+1] + = 212.3.
Step 10: Example 27m [00440] To a solution of Example 27l (121 mg, 0.37 mmol, 1.0 eq) and DIEA (239 mg, 1.85 mmol, 5.0 eq) in DCM (30 mL) was added HATU (167 mg, 0.44 mmol,1.2 eq). After stirring for 10 min, Example 27k (crude 105 mg, 0.37 mmol,1.0 eq) was added, which was stirred for 2 h at r.t. The mixture was concentrated in vacuo. The residue was purified by silica gel flash column chromatography to afford the product Example 27m (65 mg, 33.8% yield) as a white solid. LCMS [M+1] + = 520.3.
Step 11: Example 27n
[00441] To a solution of Example 27m (60 mg, 0.12 mmol, 1.0 eq) in dioxane (2 mL) were added Cs 2 CO 3 (75.1 mg, 0.24 mmol, 2.0 eq) and 3 rd t-Bu-XphosPd (10.2 mg, 0.012 mmol, 0.1 eq). The reaction mixture was stirred for 3 h at 80 o C under N 2 . The reaction solution was filtered and the filtrate was concentrated in vacuo. The crude product was purified by prep-TLC to afford the desired product
Example 27n (25 mg, 44.7% yield) as a white solid. LCMS [M+1] + = 484.4.
Step 12: Example 27
[00442] To a solution of Example 27n (25 mg, 0.052 mmol, 1.0 eq) in DCM (2 mL) was added HCl/dioxane (0.2 mL, 4 mol/L dioxane) at 0 o C, which was stirred for 2 h at r.t. The reaction solution was concentrated in vacuo. The crude product was dissolved in MeOH, and Na2CO 3 (excess) was added. The resulting mixture was stirred for 10 min at r.t. and the precipitate was filtered. The filtrate was concentrated, and the residue was purified by pre-TLC to afford the desired product Example 27 (7.2 mg, 36.4% yield) as a white solid. LCMS [M+1] + = 384.3. 1 H NMR (300 MHz, DMSO-d6) d 9.31 (s, 1H), 8.67 (s, 1H), 8.25 (d, 1H), 8.20 (d, 1H),8.02-8.04 (m, 1H),6.13 (s, 1H), 4.62 (d, 2H), 4.47 (d, 2H),3.98 (s, 3H), 3.89-3.96 (m, 1H), 3.54-3.62 (m, 1H), 3.32 -3.45 (m, 1H ), 2.93 (d, 3H),1.19 (d, 3H). Example 28:
Step 1: Example 28b
[00443] To a solution of Example 28a (20.0 g, 0.12 mol, 1.0 eq) in CCl 4 (400 mL) were added NBS (72.4 g, 0.41 mol, 3.5 eq) and AIBN (13.4 g, 0.08 mol, 0.7 eq). The reaction mixture was stirred at 80°C for 16 h. After cooled to room temperature, the solid was filtered out, and the filtrate was concentrated. The residue was purified by silica gel flash column chromatography to afford the product Example 28b (15.1 g, 52% yield) as a yellow solid. LCMS [M+1] + = 251.2.
Step 2: Example 28d
[00444] To a solution of Example 28c (12.7 g, 72.5 mmol, 1.2 eq) in THF (400 mL) was added NaH (2.9 g, 60% in mineral oil, 72.5 mmol, 1.2 eq) in portions at 0°C. The mixture was stirred for 5 min at the same temperature, then Example 28b (15.1 g, 60.4 mmol, 1.0 eq) in THF (50 mL) was added dropwise. The reaction mixture was stirred at r.t. for 1 h. The reaction was quenched with H 2 O (100 mL) and extracted with EtOAc (200 mL*3). The combined organic layers were washed with brine, dried over Na 2 SO 4 and concentrated. The residue was purified by silica gel chromatography to afford the desired product Example 28d (5.2 g, 25% yield) as yellow oil. LCMS [M+1] + = 346.3.
Step 3: Example 28e
[00445] To a solution of Example 28d (5.1 g, 14.7 mmol, 1.0 eq) in t-BuOH/H 2 O (100 mL/30 mL) was added NaOH (2.9 g,73.7 mmol, 5.0 eq). The mixture was stirred for 16 h at 80°C. After cooled to r.t., the mixture was acidified with 0.2 M HCl aqueous solution, which was then extracted with mixed solvent of DCM/MeOH (200 mL*3, v/v = 10/1). The combined organic layers were washed with brine, dried over Na 2 SO 4 and concentrated. The residue was purified by silica gel chromatography to afford the desired product Example 28e (650 mg, 14% yield) as a yellow solid. LCMS [M+1] + = 328.3.
Step 4: Example 28g
[00446] To a solution of Example 28e (600 mg, 1.84 mmol, 1.0 eq) and Example 28f (580 mg, 3.67 mmol, 2.0 eq) in DMF (12 mL) were added CuI (348.6 mg, 1.84 mmol, 1.0 eq), 1,10-phenanthroline (182 mg, 0.92 mmol, 0.5 eq) and K 3 PO 4 (778 mg, 3.67 mmol, 2.0 eq). The mixture was stirred at 110°C for 16 h under N 2 . The reaction solution was diluted with EtOAc (100 mL), washed with brine (100 mL*3), dried over Na 2 SO 4 and concentrated. The residue was purified by silica gel chromatography to afford the desired product Example 28g (120 mg, 16.2% yield) as a yellow solid. LCMS [M+1] + = 405.3.
Step 5: Example 28h
[00447] To a solution of Example 28g (115 mg, 0.284 mmol, 1.0 eq) in EtOH (2.2 mL) and water (0.7 mL) were added Zn (92.5 mg, 1.423 mmol, 5.0 eq), and NH 4 Cl (76.8 mg, 1.423 mmol, 5.0 eq). The reaction mixture was stirred for 1 h at 80 o C. After cooled to room temperature, the mixture was filtered, and the filtrate was concentrated. The crude product was purified by silica gel flash column
chromatography to afford the product Example 28h (85 mg, 80% yield) as a yellow solid. LCMS [M+1] + = 375.3.
Step 6: Example 28i
[00448] To a solution of Example 28h (80 mg, 0.214 mmol, 1.0 eq) in DCM (1 mL) was added HCl/dioxane (0.3 mL, 4M in dioxane), which was stirred at r.t. for 1 h. After the reaction was completed, the solvent was concentrated to give Example 28i (70 mg, crude) as a yellow solid. The crude was used next step directly without further purification. LCMS [M+1] + = 275.3.
Step 7: Example 28k
[00449] To a solution of Example 28j (57 mg, 0.175 mmol, 0.8 eq) in DCM (1 mL) were added HATU (99.8 mg, 0.263 mmol, 1.2 eq) and DIEA (113 mg, 0.876 mmol, 4.0 eq). The mixture was stirred for 20 min, then Example 28i (60 mg, 0.219 mmol, 1.0 eq) was added. The reaction mixture was stirred at r.t. for 2 h. The solution was concentrated in vacuum, the crude was purified by Prep-TLC to afford the product Example 28k (70 mg, 69% yield) as a yellow solid. LCMS [M+1] + = 583.3.
Step 8: Example 28l
[00450] To a solution of Example 28k (70 mg, 0.12 mmol, 1.0 eq) in dioxane (1 mL) were added Cs 2 CO 3 (78 mg, 0.24 mmol, 2.0 eq) and 3 rd t-Bu-Xphos-Pd (11 mg, 0.012 mmol, 0.1 eq). The reaction mixture was stirred for 4 h at 80 o C under N2 protection. The solid was filtered out, and the filtrate was concentrated. The residue was purified by Prep-TLC to afford the Example 28l (45 mg, 69% yield) as a yellow solid. LCMS [M+1] + =547.3.
Step 9: Example 28
[00451] To a solution of Example 28l (40 mg, 0.073 mmol, 1.0 eq) in DCM (1 mL) was added TFA (0.3 mL) dropwise at 0 o C. The reaction mixture was stirred for 2 h at r.t. After completion, the reaction mixture was concentrated. The crude product was dissolved in MeOH (2 mL) and basified with NaHCO 3 . The solid was filtered out and filtrate was concentrated. The residue was purified by Prep-TLC to afford the Example 28 (4.2 mg, 13% yield) as a yellow solid. LCMS [M+1] + = 447.1. 1 H NMR (300 MHz, D MSO-d 6 )d 9.19 (s, 1H), 8.64 (d, 1H), 8.48 (d, 1H), 8.22-8.15 (m, 2H), 8.08-8.01 (m, 1H), 7.96-7.90 (m, 1H), 7.86 (d, 1H), 7.59-7.51 (m, 2H), 6.11 (s, 1H), 4.43 (s, 2H), 4.06-3.93 (m, 1H), 3.63-3.48 (m, 2H), 2.92 (d, 3H), 1.19 (d, 3H).
Example 29:
Step 1: Example 29b
[ 00452] A solution of Example 29a (2.40 g, 12.57 mmol, 1.0 eq) in CCl 4 (100 mL) was heated to 80 oC, followed by addition of NBS (2.68 g, 15.08 mmol, 1.2 eq) and AIBN (2.06 g, 12.57 mmol, 1.0 eq). The reaction mixture was stirred for 4 h at 80 o C. The reaction solution was concentrated and purified by silica gel flash column chromatography to afford the desired product Example 29b (2.16 g, 63.7% yield) as a yellow solid.
Step 2: Example 29d
[00453] To a solution of Example 29c (2.80 g, 16.00 mmol, 2.0 eq) in THF (100 mL) was added NaH (480 mg, 60% in mineral oil, 12.00 mmol, 1.5 eq) in portions at 0 o C. After stirring for 30 min, Example 29b (2.16 g, 8.00 mmol, 1.0 eq) was added, which was stirred for another 2 h at r.t. The reaction was quenched with saturated aqueous NH 4 Cl and extracted with EtOAc (100 mL*3). The combined organic layers were washed with brine, dried over Na 2 SO 4 and concentrated. The residue was purified by silica gel flash column chromatography to afford the desired product Example 29d (1.27 g, 43.6% yield) as a yellow solid. LCMS [M+1-56] + = 309.2.
Step 3: Example 29e [00454] Example 29d (1.27 g, 0.84 mmol, 1.0 eq) was dissolved in MeOH (30 mL), and 10% Pd/C (500 mg) was added in portions under N 2 protection. The system was evacuated and then refilled with hydrogen. The mixture solution was stirred for 1 h at r.t. under H 2 balloon. The reaction mixture was filtered through a Celite pad and the filtrate was concentrated to afford the desired product Example 29e (1.14 g, 97.8% yield) as yellow oil. LCMS [M+1+22] + = 357.3.
Step 4: Example 29f
[00455] To a solution of Example 29e (1.14 g, 3.41 mmol, 1.0 eq) in DCM (30 mL) was added HCl/dioxane (10 mL, 4 M in dioxane). The reaction solution was stirred for 0.5 h at r.t., and the solvent was removed to afford the crude desired product Example 29f (1.64 g, quant. yield) as a yellow solid. LCMS [M+1] + = 235.3.
Step 5: Example 29h
[00456] To a solution of Example 29g (300 mg, 0.92 mmol, 1.0 eq) in DCM (15 mL) were added DIEA (947 mg, 7.34 mmol, 8.0 eq) and HATU (383 mg, 1.01 mmol, 1.1 eq). After stirring for 30 min, Example 29f (373 mg, 1.38 mmol, 1.5 eq) was added. The reaction solution was stirred for 2 h at r.t. The reaction mixture was concentrated and purified by silica gel flash column chromatography to afford the desired product Example 29h (280 mg, 56.2% yield) as a yellow solid. LCMS [M+1] + = 543.3.
Step 6: Example 29i
[00457] To a solution of Example 29h (240 mg, 0.44 mmol, 1.0 eq) in dioxane (10 mL) were added Cs 2 CO 3 (288 mg, 0.88 mmol, 2.0 eq) and 3 rd -t-Bu-Xphos-Pd (39 mg, 0.044 mmol, 0.1 eq). The reaction mixture was stirred for 16 h at 80 o C under N2. The reaction mixture was concentrated and purified by prep-TLC to afford the desired product Example 29i (75 mg, 33.5% yield) as a yellow solid. LCMS [M+1] + = 507.3.
Step 7: Example 29
[00458] To a solution of Example 29i (65 mg, 0.13 mmol, 1.0 eq) in DCM (6 mL) was added
HCl/dioxane (3 mL, 4 M in dioxane). The reaction solution was stirred for 1 h at r.t. and concentrated. The crude product was dissolved in MeOH, and Na2CO 3 solid (excess) was added to the mixture, which was stirred for 10 min at r.t. The mixture was filtered and the filtrate was concentrated. The residue was purified by prep-TLC to afford the desired product Example 29 (33.8 mg, 64.8% yield) as an off-white solid. LCMS [M+1] + = 407.3. 1 H NMR (300 MHz, DMSO-d 6 ) d 9.60 (s, 1H), 8.39 (d, 1H), 8.31 (s, 1H), 8.23 (d, 1H), 8.13 (s, 1H), 7.84 (d, 1H), 7.22 (s, 1H), 6.08 (s, 1H), 4.65 (d, 1H), 4.51 (d, 1H), 4.20 (s, 3H), 4.01 - 3.90 (m, 1H), 3.51 (d, 1H), 3.38 (d, 1H), 2.93 (d, 3H), 1.13 (d, 3H).
Example 30 & Example 31
Step 1: Example 30b
[00459] To a solution of Example 30a (2.6 g, 15.0 mmol) in THF (30 mL) was drop-wised MeLi (18.7 mL, 1.6 moL/L) at -78 o C under N2 protection. The reaction mixture was stirred at -78 o C for 2 h. Then the mixture was quenched by NH 4 Cl (aq.), diluted by DCM, washed by water, and dried over anhydrous Na 2 SO 4 . The solution was concentrated under reduced pressure and the residue was purified by silica gel column chromatography to give Example 30b (1.8 g, yield: 63%) as a white solid.
Step 2: Example 30d
[00460] To a solution of Example 30b (380 mg, 2.0 mmol), TBAI (75 mg, 0.2 mmol) in THF (5 mL) was added NaH (173 mg, 60% in mineral oil, 3.0 mmol) at 0 o C. The reaction mixture was warmed to room temperature and stirred for 0.5 h. Then Example 30c (492 mg, 2.0 mmol) was added. The mixture was stirred at r.t. for another 6 h. The reaction mixture was quenched by aq. NH 4 Cl, extracted by EtOAc, and dried over anhydrous Na 2 SO 4 . The solution was concentrated under reduced pressure. The residue was purified by silica gel column chromatography to give Example 30d (230 mg, yield: 32%) as a yellow solid. LCMS [M+1-100] + = 255.1.
Step 3: Example 30e
[00461] To a solution of Example 30d (230 mg, 0.65 mmol) and 10% Pd/C (50 mg) in MeOH (10 mL) was stirred at r.t. for 2 h under 1 atm H 2 . After completion, the mixture was filtered over a Celite, and the filtrate was concentrated under reduced pressure. The residue Example 30e (178 mg, yield: quant.) was obtained as a yellow solid, which was used in the next step directly. LCMS [M-188] + = 137.1
Step 4: Example 30f To a solution of Example 30e (178 mg, 0.75 mmol) in DCM (4 mL) was added TFA (1.0 mL), which was stirred at r.t. for 2 h. The mixture was concentrated to give the crude product Example 30f (138 mg, crude, yield: 82%) as black oil. LCMS [M+1] + = 225.1.
Step 5: Example 30h
[00462] To a solution of Example 30f (138 mg, 0.62 mmol), Example 30g (200 mg, 0.62 mmol), and TEA (311 mg, 3.1 mmol) in DCM (5 mL) was added HATU (236 mg, 0.62 mmol). The reaction mixture was stirred at r.t. for 2 h. Then, DCM (40 mL) was added to the reaction mixture, which was washed with brine (20 mL*2), dried over anhydrous Na 2 SO 4 and concentrated. The residue was purified by silica gel column chromatography to afford the desired product Example 30h (150 mg, yield: 45%) as a brown solid. LCMS [M+1] + = 533.2
Step 6: Example 30i
[00463] To a mixture of Example 30h (150 mg, 0.28 mmol), Cs 2 CO 3 (137 mg, 0.42 mmol) in dioxane (2 mL) was added 3rd-t-Bu-Xphos-Pd (25 mg, 0.028 mmol). The mixture was degassed with N 2 three times, and stirred for 3 h at 80°C. Then the reaction mixture diluted by DCM, washed by water, dried over anhydrous Na 2 SO 4 , and then concentrated under reduced pressure to afford crude Example 30i (170 mg, crude, yield: quant.) as a white solid, which was used in the next step without further purification. LCMS [M+1] + = 497.2.
Step 7: Example 30 & Example 31
[00464] To a solution of Example 30i (120 mg, crude, 0.34 mmol) in DCM (4 mL) was added TFA (1.0 mL), which was stirred at r.t. for 2 h. The mixture was concentrated, and the residue was purified by Prep-HPLC to afford the desired products:
Example 30 (6.7 mg, R.T. = 1.743 min, yield: 5%) as a white solid. LCMS [M+1] + = 397.2. 1 H NMR (400 MHz, DMSO-d6) d 8.81 (s, 1H), 8.54 (s, 1H), 8.15 (d, 1H), 8.10 (s, 1H), 7.77 (d, 1H), 6.89 (q, 2H), 5.98 (s, 1H), 4.58 (d, 1H), 4.31 (d, 1H), 3.84 (s, 3H), 3.81 (d, 1H), 3.73 (d, 1H), 3.28 (s, 1H), 2.88 (d, 3H), 1.17 (d, 3H), 1.08 (d, 3H).
& Example 31 (3.1 mg, R.T. = 1.652 min, yield: 2%) as a white solid. LCMS [M+1] + = 397.2
1H NMR (400 MHz, DMSO-d6) d 8.94 (s, 1H), 8.33 (s, 1H), 8.15 (s, 1H), 8.07 (s, 1H), 7.79 (s, 1H), 6.92 (d, 2H), 5.85 (s, 1H), 4.56 (d, 1H), 4.39 (d, 1H), 3.84 (s, 3H), 3.59 (s, 1H), 2.88 (d, 3H), 1.12 (d, 3H), 1.03 (d, 3H). Example 32 & Example 33
Step 1: Example 32b
[00465] To a solution of DMSO (3.3 mL, 47.9 mmol) in DCM (10 mL) was added (COCl) 2 (3.0 mL, 34.2 mmol) at -78 o C, which was stirred at -78 o C for 15 min. Then Example 32a (3.0 g, 17.1 mmol) in THF (2 mL) was added dropwise, which was stirred at -78 o C for 2 h. Then TEA (3.3 mL, 85.6 mmol) was added dropwise and the resulting mixture was stirred at -78 o C for 0.5 h. The reaction mixture was quenched by brine, diluted by DCM, washed by water, and dried over anhydrous Na 2 SO 4 . The solution was concentrated under reduced pressure, which was purified by silica gel column chromatography to give Example 32b (2.1 g, yield: 71%) as a white solid.
Step 2: Example 32c
[00466] To a solution of Example 32b (1.2 g, 6.9 mmol) in THF (10 mL) was added MeLi (10.8 mL, 17.3 mmol, 1.6 moL/L) dropwise at -78 o C under N 2 protection. The reaction mixture was stirred at -78 o C for 2 h. Then the reaction mixture was quenched by aq. NH 4 Cl, diluted by DCM, washed by water, and dried over anhydrous Na 2 SO 4 . The solution was concentrated under reduced pressure, which was purified by silica gel column chromatography to give Example 32c (600 mg, yield: 46%) as a white solid.
Step 3: Example 32e
[00467] To a solution of Example 32c (500 mg, 2.64 mmol), TBAI (96 mg, 0.26 mmol) in THF (5 mL) was added NaH (127 mg, 60% in mineral oil, 5.28 mmol) at 0 o C in portions. The reaction mixture was warmed to room temperature and stirred at r.t. for 0.5 h. Then Example 32d (780 mg, 3.17 mmol) was added. The mixture was stirred at r.t. for overnight. The reaction mixture was quenched by aq. NH 4 Cl, and then extracted by EtOAc, and dried over anhydrous Na 2 SO 4 . The solution was concentrated under reduced pressure and purified by silica gel column chromatography to give Example 32e (160 mg, yield: 18%) as a yellow solid. LCMS [M+1-100] + = 255.1.
Step 4: Example 32f [00468] A mixture of Example 32e (160 mg, 0.45 mmol) and 10% Pd/C (20 mg) in MeOH (10 mL) was stirred at r.t. for 2 h under 1 atm of H 2 . Then the suspension was filtered, and the organic phase was concentrated under reduced pressure to give crude Example 32f (140 mg, yield: quant.) as a yellow solid, which was used in the next step directly. LCMS [M-188] + = 137.1.
Step 5: Example 32g
[00469] To a solution of Example 32f (140 mg, 0.43 mmol) in DCM (4 mL) was added TFA (2.0 mL), which was stirred at r.t. for 2 h. The mixture was concentrated to give the crude product Example 32g (100 mg crude, yield: quant.) as black oil. LCMS [M+1] + = 225.1.
Step 6: Example 32i
[00470] To a solution of Example 32g (97 mg, 0.43 mmol), Example 32h (141 mg, 0.43 mmol), TEA (112 mg, 0.86 mmol) in DCM (5 mL) was added HATU (246 mg, 0.65 mmol). The reaction mixture was stirred at r.t. for 2 h. Then DCM (40 mL) was added to the reaction mixture, which was washed with brine (20 mL*2), dried over anhydrous Na 2 SO 4 and concentrated. The residue was purified by silica gel column chromatography to afford the desired product Example 32i (110 mg, yield: 48%) as a brown solid. LCMS [M+1] + = 533.2.
Step 7: Example 32j
[00471] To a mixture of Example 32i (110 mg, 0.21 mmol), Cs 2 CO 3 (134 mg, 0.41 mmol) in dioxane (2 mL) was added 3rd-t-Bu-Xphos-Pd (17 mg, 0.021 mmol). The mixture was degassed with N2 three times, and stirred for 3 h at 80°C. Then the reaction mixture diluted by DCM, washed by water, dried over anhydrous Na 2 SO 4 , and then concentrated under reduced pressure to afford crude Example 32j (100 mg crude, yield: 97%) as a white solid, which was used in the next step without further purification. LCMS [M+1] + = 497.2.
Step 8: Example 32 & Example 33
[00472] To a solution of Example 32j (100 mg, 0.201 mmol) in DCM (4 mL) was added TFA (1.0 mL), which was stirred at r.t. for 2 h. The mixture was concentrated and neutralized by aq NaHCO 3 , extracted by DCM, washed by water, dried over anhydrous Na 2 SO 4 , and then concentrated under reduced pressure. The residue was purified by Prep-HPLC to afford the desired product Example 32 (5.7 mg, yield: 16%) as a white solid. As Peak 1: LCMS [M+1] + = 397.2; Rt = 1.609 min. 1 H NMR (400 MHz, DMSO-d 6 ) d 8.97 (s, 1H), 8.34 (d, 1H), 8.16 (d, 1H), 8.08 (s, 1H), 7.81 (d, 1H), 6.98-6.88 (m, 2H), 5.86 (s, 1H), 4.57 (d, 1H), 4.40 (d, 1H), 3.85 (s, 3H), 3.59 (q, 1H), 3.25-3.15 (m, 1H), 1.13 (d, 3H), 1.04 (d, 3H).
& Example 33 (5.1 mg, yield: 13%) as a white solid. As Peak 2: LCMS [M+1] + = 397.2; Rt = 1.675 min. 1 H NMR (400 MHz, Methanol-d 4 ) d 8.64-8.56 (m, 1H), 8.42 (s, 1H), 8.15 (s, 1H), 6.89 (t, 2H), 5.77 (s, 1H), 5.49 (s, 1H), 4.65 (d, 1H), 4.36 (d, 1H), 3.91 (s, 4H), 3.82 (d, 1H), 3.02 (s, 3H), 1.23 (d, 6H).
Example 34:
Step 1: Example 34b
[00473] To a solution of Example 34a (15.0 g, 54.0 mmol) in DMF (225 mL) was added SnCl2 (35.8 g, 189.0 mmol). The mixture was degassed with N 2 three times, and stirred for 1 h at 25°C. Then water was added, and the organics were extracted with EtOAc for 3 times. The combined organics were dried over anhydrous Na 2 SO 4 , and then concentrated under reduced pressure, which was purified by silica gel column chromatography to afford the desired product Example 34b (8.1 g, yield: 61%) as a red solid. LCMS [M+1] + = 246.0.
Step 2: Example 34d
[00474] To a solution of Example 34a (8.1 g, 32.5 mmol) in Example 34c (100 mL) was added TsOH.H 2 O (627 mg, 3.3 mmol). The mixture was degassed with N 2 three times, and stirred for 1 h at 80°C. The mixture was concentrated under reduced pressure and purified by silica gel column chromatography to afford the desired product Example 34d (5.5 g, yield: 61%) as a yellow solid. LCMS [M+1] + = 269.9.
Step 3: Example 34e
[00475] To a solution of Example 34d (5.97 g, 22.10 mmol) in THF (90 mL) was added LiAlH 4 (1.00 g, 26.47 mmol) at -20°C. The mixture was degassed with N 2 three times, and stirred for 1 h at -20°C. The reaction was then quenched by the addition of water (1.2 mL) at -20°C. The resulting solution was diluted with aqueous NaOH solution (15%, 3.6 mL) and EtOAc (1.2 mL) at room temperature. The solids were filtered out. The filtrate was concentrated under reduced pressure and purified by silica gel column chromatography to afford the desired product Example 34e (2.31 g, yield: 46%) as a yellow solid.
LCMS [M+1] + = 241.9.
Step 4: Example 34f
[00476] To a solution of Example 34e (2.31 g, 9.79 mmol) in DCM (45 mL) was added PPh3 (3.30 g, 12.70 mmol). The mixture was cooled to 0°C. Then a solution of CBr4 (4.20 g, 12.70 mmol) in DCM (5 mL) was added dropwise. After addition, the reaction mixture was stirred for 2 h at 0°C. The mixture was concentrated under reduced pressure and purified by silica gel column chromatography to afford the desired product Example 34f (2.51 g, yield: 82%) as a yellow solid. LCMS [M+1] + = 305.9
Step 5: Example 34h
[00477] To a solution of Example 34f (2.20 g, 7.26 mmol), Example 34g (1.52 g, 8.59 mmol) in THF (40 mL) was added NaH (348 mg, 60% in mineral oil, 8.59 mmol). The mixture was degassed with N 2 three times, and stirred for 2 h at 25°C. The reaction was then quenched by addition of aqueous NH 4 Cl solution (10 mL) and the organics were extracted with EtOAc for 3 times. The combined organics was dried over anhydrous Na 2 SO 4 , concentrated under reduced pressure and purified by silica gel column chromatography to afford the desired product Example 34h (1.20 g, yield: 75%) as yellow oil. LCMS [M+1-100] + = 299.0.
Step 6: Example 34i
[00478] To a solution of Example 34h (820 mg, 2.06 mmol), NH 2 Boc (480 mg, 4.12 mmol), Cs 2 CO 3 (1340 mg, 4.12 mmol) in dioxane (5.0 mL) were added Pd 2 (dba) 3 (189 mg, 0.21 mmol), and Xantphos (123 mg, 0.21 mmol). The mixture was degassed with N2 three times, and stirred for overnight at 95°C. Then the reaction mixture diluted by EtOAc, washed by water, dried over anhydrous Na 2 SO 4 , and then concentrated under reduced pressure. The residue was purified by silica gel column chromatography to afford the desired product Example 34i (520 mg, yield: 58%) as a white solid. LCMS [M+1-100] + = 336.2.
Step 7: Example 34j
[00479] To a solution of Example 34i (170 mg, 0.55 mmol) in DCM (5.0 mL) was added TFA (1.0 mL), which was stirred at r.t. for 2 h. The mixture was concentrated to give the crude product Example 34j (348 mg crude, quant. yield) as black oil. LCMS [M+1] + =236.1
Step 8: Example 34l
[00480] To a solution of Example 34j (348 mg crude, 0.75 mmol), Example 34k (245 mg, 0.75 mmol), TEA (755 mg, 7.50 mmol) in DCM (4 mL) was added HATU (284 mg, 0.75 mmol). The reaction mixture was stirred at r.t. for 2 h. Then DCM (40 mL) was added to the reaction mixture, which was washed with brine (20 mL*2), dried over anhydrous Na 2 SO 4 and concentrated. The residue was purified by silica gel column chromatography to afford the desired product Example 34l (70 mg, yield:17%) as a brown solid. LCMS [M+1] + = 544.2.
Step 9: Example 34m
[ 00481] To a mixture of Example 34l (70 mg, 0.13 mmol), Cs 2 CO 3 (65 mg, 0.20 mmol) in dioxane (2.0 mL) was added 3rd-t-Bu-Xphos-Pd (12 mg, 0.013 mmol). The mixture was degassed with N 2 three times, and stirred for 3 h at 80°C. Then the reaction mixture was diluted by EtOAc, washed by water, dried over anhydrous Na 2 SO 4 , and then concentrated under reduced pressure to afford crude Example 34m (60 mg crude, quant. Yield) as a white solid, which was used in the next step without further purification. LCMS [M+1] + = 508.2
Step 10: Example 34 [00482] To a solution of Example 34m (60 mg crude, 0.49 mmol) in THF (1.4 mL) was added MeOH/HCl (2.0 mL, 6.0 moL/L), which was stirred at r.t. for 2 h. The mixture was concentrated, and the residue was purified by Prep-HPLC to afford the desired product Example 34 (1.2 mg, yield: 3%) as a white solid. LCMS [M+1] + = 408.1. 1 H NMR (400 MHz, DMSO-d 6 ) d 9.93 (s, 1H), 8.38 (d, 1H), 8.22 (d, 1H), 8.13 (s, 1H), 7.97 (d, 1H), 7.21 (s, 1H), 5.82 (s, 1H), 4.68 (d, 1H), 4.54 (d, 1H), 3.89 (d, 1H), 3.49 (d, 1H), 3.29 (s, 1H), 2.92 (d, 3H), 2.63 (s, 3H), 1.12 (d, 3H).
Example 35:
Step 1: Example 35b
[00483] To a solution of Example 35a (20.0 g, 86.6 mmol, 1.0 eq) in EtOH (120 mL) and conc. HCl (40 mL) was added SnCl2 (97.4 g, 433 mmol, 5.0 eq). The reaction mixture was stirred at 60°C for 3 h under N2. After cooled to room temperature, the mixture was poured into 2M NaOH aqueous solution (750 mL) at 0ºC. DCM (800 mL) was added to the mixture, and the white solid was removed by filtration. The organic layer was separated, and the aqueous phase was extracted with DCM (500 mL*2). The combined organic extracts were washed with brine, dried over Na2SO4, and concentrated. The crude was purified by silica gel flash column chromatography to afford the product Example 35b (16.8 g, 97% yield) as a yellow solid. LCMS [M+1] + = 201.2.
Step 2: Example 35c
[ 00484] A solution of Example 35b (12.0 g, 59.7 mmol, 1.0 eq) in AcOH (115 mL) and H 2 O (33 mL) was cooled to 0ºC, followed by the addition of NaNO 2 (4.9 g, 71.6 mmol, 1.2 eq) in water (20 mL). The reaction mixture was stirred for 2 h at r.t. After completion, a gradual formation of a yellow precipitate was observed. The solid was collected by filtration and concentrated to afford the product Example 35c (12.5 g, 99% yield) as a yellow solid.
LCMS [M+1] + =212.2.
Step 3: Example 35d
[00485] To a solution of Example 35c (12.7 g, 60.0 mmol, 1.0 eq) in ACN (130 mL) were added K 2 CO 3 (16.6 g, 120.0 mmol, 2.0 eq) and MeI (25.6 g, 180.0 mmol, 3.0 eq). The reaction mixture was stirred at 60°C for 16 h. After cooled to room temperature, the solid was filtered out and filtrate was concentrated. The crude product was purified by silica gel flash column chromatography to afford the product Example 35d (3.2 g, 24% yield, retention time: 1.48 min) as a white solid, Example 35d1 (2.5 g, 18% yield, retention time: 1.42 min) as a white solid, and Example 35d2 (3.4 g, 25% yield, retention time: 1.33 min) as a white solid. LCMS [M+1] + = 226.2.
Step 4: Example 35e
[00486] To a solution of Example 35d (1.5 g, 6.6 mmol, 1.0 eq) in CCl 4 (30 mL) was added NBS (1.76 g, 9.9 mmol, 1.5 eq) and AIBN (541.2 mg, 3.3 mmol, 0.5 eq). The reaction mixture was stirred at 80°C for 6 h. After cooled to room temperature, the solvent was removed, and the residue was purified by silica gel flash column chromatography to afford the product Example 35e (1.5 g, 74% yield) as a white solid. LCMS [M+1] + = 306.2.
Step 5: Example 35g
[00487] To a solution of Example 35f (875 mg, 5.0 mmol, 1.5 eq) in THF (20 mL) were added NaH (160 mg, 4.0 mmol, 1.2 eq) in portions at 0°C. The mixture was stirred for 30 min at the same temperature, then Example 35e (1.0 g, 3.3 mmol, 1.0 eq) in THF (15 mL) was added dropwise. The reaction mixture was stirred at r.t. for 16 h. The mixture was poured into a saturated aqueous solution of NH 4 Cl (50 mL), which was extracted with EtOAc (50 mL*3). The combined organic layers were washed with brine, dried over Na 2 SO 4 , and concentrated. The crude product was purified by silica gel flash column chromatography to afford the product Example 35g (860 mg, 65% yield) as a white solid. LCMS [M+1] + = 399.3.
Step 6: Example 35h
[00488] To a solution of Example 35g (860 mg, 2.16 mmol, 1.0 eq) in dioxane (10 mL) were added Cs 2 CO 3 (1.4 g, 4.32 mmol, 2.0 eq), NH 2 -Boc (505.4 mg, 4.32 mmol, 2.0 eq), Xantphos (250.0 mg, 0.43 mmol, 0.2 eq) and Pd2(dba)3.CHCl 3 (227.7 mg, 0.22 mmol, 0.1 eq). The reaction mixture was stirred for 2 h at 110 o C under N2 protection. After cooled to room temperature, the solvent was removed. The crude product was purified by silica gel flash column chromatography to afford the product Example 35h (670 mg, 71% yield) as yellow oil. LCMS [M+1] + = 436.4.
Step 7: Example 35i
[00489] To a solution of Example 35h (670 mg, 1.54 mmol, 1.0 eq) in DCM (5 mL) was added TFA (2.5 mL) dropwise at 0 o C. The reaction mixture was stirred for 2 h at r.t. The solution was concentrated in vacuum to give the crude product Example 35i (1.2 g, crude)as yellow oil, which was used to next step directly without purification. LCMS [M+1] + = 236.2.
Step 8: Example 35k
[00490] To a solution of Example 35j (817.5 mg, 2.5 mmol, 0.8 eq) in DCM (20 mL) were added HATU (2.28 g, 6.0 mmol, 1.2 eq) and DIEA (5.16 g, 40.0 mmol, 8.0 eq). The mixture was stirred for 20 min, then Example 35i (1.2 g, 5.0 mmol, 1.0 eq) was added. The reaction mixture was stirred at r.t. for 2 h. The solution was concentrated in vacuum to give the crude product was purified by silica gel flash column chromatography to afford the product Example 35k (260 mg, 19% yield) as a yellow solid. LCMS [M+1] + = 544.4.
Step 9: Example 35l
[00491] To a solution of Example 35k (260 mg, 0.48 mmol, 1.0 eq) in dioxane (3 mL) were added Cs 2 CO 3 (313.0 mg, 0.96 mmol, 2.0 eq) and 3 rd -t-Bu-Xphos-Pd (44.1 mg,0.05 mmol, 0.1 eq). The reaction mixture was stirred for 12 h at 80 o C under N 2 protection. The solid was filtered out and filtrate was concentrated. The residue was purified by Prep-TLC to afford the Example 35l (75 mg, 31%yield) as a yellow solid. LCMS [M+1] + = 508.3.
Step 10: Example 35
[00492] To a solution of Example 35l (75 mg, 0.15 mmol, 1.0 eq) in DCM (3 mL) was added
HCl/dioxane (3mL, 4M in dioxane) dropwise at 0 o C. The reaction mixture was stirred for 2 h at r.t. After completion, the reaction mixture was concentrated. The crude product was dissolved in MeOH (2 mL), and NaHCO 3 (excess) was added. The mixture was stirred for 20 min at r.t., and then DCM (20 mL) was added. The solid was filtered out and filtrate was concentrated. The residue was purified by Prep-TLC to afford the Example 35 (22.7 mg, 37% yield) as an off-white solid. LCMS [M+1] + = 408.2. 1 H NMR (300 MHz, DMSO-d6) d 10.05 (s, 1H), 8.47 (s, 1H), 8.36 (d, 1H), 8.17 (s, 1H), 7.96 (d, 1H), 7.41 (d,1H), 6.06 (s, 1H), 4.76 (d, 1H), 4.65 (d, 1H), 4.53 (s, 3H), 3.99-3.95 (m, 1H), 3.59-3.56 (m, 1H), 3.44-3.38 (m, 1H), 2.95 (d, 3H), 1.16 (d, 3H).
Example 36:
Step 1: Example 36b
[00493] To a solution of Example 36a (1.2 g, 5.0 mmol, 1.0 eq) in CCl4 (12 mL) were added NBS (1.42 g, 8.0 mmol, 1.5 eq) and AIBN (262 mg,1.6 mmol, 0.3 eq). The reaction mixture was stirred at 80°C for 6 h. After cooled to room temperature, the solvent was removed, and the residue was purified by silica gel flash column chromatography (to afford the product Example 36b (850 mg, 52% yield) as a yellow solid. LCMS [M+1] + = 306.2.
Step 2: Example 36d
[00494] To a solution of Example 36c (2.15 g, 12.0 mmol, 1.5 eq) in THF (25 mL) was added NaH (490 mg, 60% in mineral oil, 12.0 mmol, 1.5 eq) in portions at 0°C. The mixture was stirred for 30 min at the same temperature, then Example 36b (2.5 g, 8.0 mmol, 1.0 eq) in THF (20 mL) was added dropwise. The reaction mixture was stirred at r.t. for 16 h. Then, the mixture was poured into a saturated aqueous solution of NH 4 Cl (50 mL), which was extracted with EtOAc (50 mL*3). The combined organic layers were washed with brine, dried over Na 2 SO 4 and concentrated. The crude product was purified by silica gel flash column chromatography to afford the product Example 36d (1.8 g, 55% yield) as an off-white solid. LCMS [M+1] + = 399.3.
Step 3: Example 36e
[00495] To a solution of Example 36d (1.8 g, 4.5 mmol, 1.0 eq) in dioxane (36 mL) were added Cs 2 CO 3 (2.95 g, 9.0 mmol, 2.0 eq), NH 2 -Boc (4.23 g, 36.0 mmol, 8.0 eq), Xantphos (130 mg, 0.23 mmol, 0.05 eq) and Pd2(dba)3 (470 mg, 0.45 mmol, 0.1 eq). The reaction mixture was stirred for 16 h at 110 o C under N2 protection. After cooled to room temperature, the solvent was removed. The crude product was purified by silica gel flash column chromatography to afford the product Example 36e (1.1 g, 56% yield) as yellow solid. LCMS [M+1] + = 436.3.
Step 4: Example 36f
[00496] To a solution of Example 36e (1.1 g, 3.0 mmol, 1.0 eq) in DCM (11 mL) was added TFA (33 mL) dropwise at 0 o C. The reaction mixture was stirred for 2 h at r.t. The solution was concentrated in vacuum to give the crude product Example 36f (1.7 g, crude) as brown oil, which was used to next step directly without purification. LCMS [M+1] + =236.4.
Step 5: Example 36h
[00497] To a solution of Example 36g (485 mg, 1.489 mmol, 0.7 eq) in DCM (10 mL) were added HATU (808 mg, 2.127 mmol, 1.0 eq) and DIEA (2.2 g, 17.021 mmol, 8.0 eq). The mixture was stirred for 20 min, and then Example 36f (500 mg, 2.127 mmol, 1.0 eq) was added. The reaction mixture was stirred at r.t. for 2 h. The solution was concentrated in vacuum, and the crude product was purified by prep-TLC to afford the product Example 36h (250 mg, 22% yield) as a yellow solid. LCMS [M+1] + = 544.3.
Step6: Example 36i
[00498] To a solution of Example 36h (250 mg, 0.46 mmol, 1.0 eq) in dioxane (2 mL) were added Cs 2 CO 3 (300 mg, 0.921 mmol, 2.0 eq), Pd 2 (dba) 3 (47 mg, 0.046 mmol, 0.1 eq) and BINAP (14 mg, 0.023 mmol, 0.05 eq). The reaction mixture was stirred for 4 h at 80 o C under N 2 protection. The solid was filtered out and filtrate was concentrated. The residue was purified by Prep-TLC to afford the Example 36i (120 mg, 52% yield) as a yellow solid. LCMS [M+1] + = 508.3. Step 7: Example 36
[00499] To a solution of Example 36i (120 mg, 0.236 mmol, 1.0 eq) in DCM (1.2 mL) was added HCl/dioxane (6 mL, 4M in dioxane) dropwise at 0 o C. The reaction mixture was stirred for 2 h at r.t. After completion, the reaction mixture was concentrated. The crude product was treated with MeOH (2 mL), NaHCO 3 (excess) was added to the solution, which was stirred for 20 minutes at r.t. Then, DCM (20 mL) was added to the mixture and the solid was filtered out. The filtrate was concentrated, and the residue was purified by Prep-TLC to afford the Example 36 (56.8 mg, 59% yield) as an off-white solid. LCMS [M+1] + = 408.2. 1 H NMR (300 MHz, DMSO-d 6 ) d 10.31 (s, 1H), 8.54 (s, 1H), 8.38 (d, 1H), 8.19 (s,1H), 7.98 (d, 1H), 7.33 (s,1H), 6.13 (s, 1H), 4.81 (d, 1H), 4.68 (d, 1H), 4.29 (s, 3H), 3.99-3.95 (m, 1H), 3.99- 3.95 (m, 1H), 3.45-3.29 (m, 1H), 2.96 (d, 3H), 1.15 (d, 3H).
Example 37:
Step 1: Example 37b
[00500] To a solution of Example 37a (1.9 g, 8.4 mmol, 1.0 eq) in CCl4 (20 mL) were added NBS (1.64 g, 9.24 mmol, 1.1 eq) and AIBN (137.8 mg, 0.84 mmol, 0.1 eq). The reaction mixture was stirred at 80°C for 6 h. After cooled to room temperature, the solvent was removed, and the residue was purified by silica gel flash column chromatography to afford the product Example 37b (1.9 g, 74% yield) as a white solid. LCMS [M+1] + = 306.2.
Step 2: Example 37d
[00501] To a solution of Example 37c (1.6 g, 9.3 mmol, 1.5 eq) in THF (20 mL) was added NaH (372 mg, 60% in mineral oil, 9.3 mmol, 1.5 eq) in portions at 0°C. The mixture was stirred for 30 min at the same temperature, then Example 37b (1.9 g, 6.2 mmol, 1.0 eq) in THF (20 mL) was added dropwise. The reaction mixture was stirred at r.t. for 16 h. Then the mixture was poured into a saturated aqueous solution of NH 4 Cl (50 mL), which was extracted with EtOAc (50 mL*3). The combined organic layers were washed with brine, dried over Na 2 SO 4 , and concentrated. The crude product was purified by silica gel flash column chromatography to afford the product Example 37d (1.5 g, 61% yield) as a white solid. LCMS [M+1] + = 399.3.
Step 3: Example 37f
[00502] To a solution of Example 37d (1.48 g, 3.7 mmol, 1.0 eq) in dioxane (14 mL) were added Cs 2 CO 3 (2.4 g, 7.4 mmol, 2.0 eq), Example 37e (2.16 g, 18.5 mmol, 5.0 eq), Xantphos (428.5 mg, 0.74 mmol, 0.2 eq) and Pd 2 (dba) 3 (383 mg, 0.37 mmol, 0.1 eq). The reaction mixture was stirred for 2 h at 110 o C under N 2 protection. After cooled to room temperature, the solvent was removed. The crude product was purified by silica gel flash column chromatography to afford the product Example 37f (1.04 g, 64% yield) as yellow oil. LCMS [M+1] + = 436.3.
Step 4: Example 37g
[00503] To a solution of Example 37f (1.04 g, 2.4 mmol, 1.0 eq) in DCM (5 mL) was added TFA (5 mL) dropwise at 0 o C. The reaction mixture was stirred for 2 h at r.t. The solution was concentrated in vacuum to give the crude product Example 37g (930 mg, crude) as yellow oil, which was used to next step directly without purification. LCMS [M+1] + =236.4
Step 5: Example 37i
[00504] To a solution of Example 37h (222 mg, 0.68 mmol, 0.8 eq) in DCM (10 mL) were added HATU (323 mg, 0.85 mmol, 1.0 eq) and DIEA (438.6 mg, 3.4 mmol, 4.0 eq). The mixture was stirred for 20 min, and then Example 37g (320 mg, 0.85 mmol, 1.0 eq) was added. The reaction mixture was stirred at r.t. for 2 h. The solution was concentrated in vacuum, and the crude product was purified by silica gel flash column chromatography to afford the product Example 37i (180 mg, 39% yield) as a yellow solid. LCMS [M+1] + = 544.3.
Step6: Example 37j
[00505] To a solution of Example 37i (100 mg, 0.18 mmol, 1.0 eq) in dioxane (2 mL) were added Cs 2 CO 3 (117.4 mg, 0.36 mmol, 2.0 eq), Pd2(dba)3 (18.6 mg, 0.018 mmol, 0.1 eq) and BINAP (22.4 mg, 0.036 mmol, 0.2 eq). The reaction mixture was stirred for 3 h at 80 o C under N2 protection. The solid was filtered out and the filtrate was concentrated. The residue was purified by Prep-TLC to afford the
Example 37j (80 mg, 88% yield) as a yellow solid. LCMS [M+1] + =508.3.
Step 7: Example 37
[00506] To a solution of Example 37j (80 mg, 0.157 mmol, 1.0 eq) in DCM (2 mL) was added HCl/dioxane (2 mL, 4M in dioxane) dropwise at 0 o C. The reaction mixture was stirred for 2 h at r.t. After completion, the reaction mixture was concentrated. The crude product was treated with MeOH (2 mL), NaHCO 3 (excess) was added to the solution, the mixture was stirred for 20 min at r.t., then DCM (20 mL) was added. The solid was filtered out and filtrate was concentrated. The residue was purified by Prep- TLC to afford the Example 37 (30.5 mg, 48% yield) as an off-white solid. LCMS [M+1] + = 408.2. 1 H NMR (300 MHz, DMSO-d 6 ) d 9.55 (s, 1H), 8.21 (s, 1H), 8.14 (s, 1H), 8.09 (d, 1H), 7.99 (d, 1H), 7.74 (d, 1H), 5.78 (s, 1H), 4.76 (d, 1H), 4.58 (d, 1H), 4.53 (s, 3H), 3.86-3.84 (m, 1H), 3.44- 3.39 (m, 1H), 3.16- 3.09 (m, 1H), 2.98 (d, 3H), 1.10 (d, 3H).
Example 38:
Step 1: Example 38c
[00507] To a solution of Example 38a (5.08 g, 30.0 mmol), Example 38b (6.76 g, 38.6 mmol), PPh3 (10.1 g, 38.5 mmol) in THF (100 mL) was added DIAD (8.30 g, 41.0 mmol). The reaction mixture was stirred at 25 o C for 10 h. Then EtOAc (400 mL) was added to the reaction mixture, which was washed with brine (100 mL*2), dried over anhydrous Na 2 SO 4 and concentrated. The residue was purified by silica gel column chromatography to give the desired product Example 38c (4.8 g, yield: 49%) as a yellow solid. LCMS [M+1] + = 327.15
Step 2: Example 38d
[00508] To a mixture of Example 38c (4.8 g, 14.7 mmol) in EtOH (79 mL) was added 10% Pd-C (500 mg). The mixture was stirred for 4 h at 25°C under H 2 atmosphere. The mixture was then concentrated under reduced pressure to afford crude Example 38d (4.44 g crude, yield: quant.) as a yellow solid. The residue was used in the next step without further purification. LCMS [M+1] + = 297.18.
Step 3: Example 38e
[00509] A solution of Example 38d (2.0 g, 6.76 mmol) in HCl-MeOH (26 mL, 3N) was stirred at r.t. for 2 h. The mixture was then concentrated under reduced pressure to afford crude Example 38e (1.6 g crude, yield: quant.) as a white solid. The residue was used at next step without further purification. LCMS [M+1] + = 197.12.
Step 4: Example 38g
[00510] To a solution of Example 38e (707 mg, 3.6 mmol), Example 38f (1.4 g, 4.3 mmol), TEA (935 mg, 9.3 mmol) in DCM (12 mL) was added HATU (2.25 g, 5.9 mmol), which was stirred at r.t. for 15 h. Then EtOAc (400 mL) was added to the reaction mixture, which was washed with brine (100 mL*2), dried over anhydrous Na 2 SO 4 and concentrated. The residue was purified by silica gel column chromatography to give the desired product Example 38g (428 mg, yield:24% over 3 steps) as a white solid. LCMS [M+1] + = 506.19.
Step 5: Example 38h [00511] To a solution of Example 38g (144 mg, 0.29 mmol), Cs 2 CO 3 (291 mg, 0.89 mmol) in dioxane (3 mL) was added 3rd-t-Bu-Xphos-Pd (16 mg, 0.02mmol), which was stirred at 80°C for 15 h under N 2 atmosphere. Then EtOAc (400 mL) was added to the reaction mixture, which was washed with brine (100 mL*2), dried over anhydrous Na 2 SO 4 and concentrated. The residue was purified by silica gel column chromatography to give the desired product Example 38h (24 mg, yield:18%) as a white solid. LCMS [M+1] + = 469.22
Step 6: Example 38
[00512] A solution of Example 38h (24 mg, 0.05 mmol) in HCl-MeOH (2 mL, 3N) was stirred at 0°C in an ice bath and then warmed up to 25°C for 6 h. Then EtOAc (400 mL) was added to the reaction mixture, which was washed with brine (100 mL*2), dried over anhydrous Na 2 SO 4 and concentrated. The residue was purified by silica gel column chromatography to give Example 38 (13 mg, yield 63%) as a white solid. LCMS [M+1] + = 369.16. 1 H NMR (400 MHz, Chloroform-d) d 8.24 (s, 1H), 7.05 (s, 1H), 6.85 (d, 1H), 6.75 (dd, 1H), 5.39 (s, 1H), 4.46-4.29 (m, 3H), 3.86 (s, 3H), 3.08 (d, 3H), 1.35 (d, 3H). Example 39:
Step 1: Example 39c
[00513] To a mixture of Example 39a (1.45 g, 10.0 mmol), Example 39b (3.55 g, 10.0 mmol), Pd 2 (dba) 3 (558 mg, 0.5 mmol), Xantphos (298 mg, 0.5 mmol), Cs 2 CO 3 (4.88 g, 15.0 mmol) in dioxane (30 mL) was degassed with N 2 three times, and stirred for 4 h at 70°C. Then the reaction mixture was cooled to r.t., diluted by DCM, washed by water, dried over anhydrous Na 2 SO 4 , and then concentrated under reduced pressure, which was purified by silica gel column chromatography to give Example 39c (3.0 g, yield: 70%) as a white solid. LCMS [M+1] + = 464.1. 1 H NMR (400 MHz, Chloroform-d) d 10.21 (s, 1H), 9.64 (s, 1H), 8.56 (s, 1H), 7.91-7.85 (m, 2H), 7.75 (d, 1H), 7.08 (s, 1H), 6.88 (d, 1H), 4.54 (q, 2H), 4.11 (q, 2H), 3.46 (s, 3H), 2.04 (s, 3H), 1.58 (s, 3H), 1.43 (s, 12H), 1.24 (d, 3H).
Step 2: Example 39d
[00514] To a solution of Example 39c (3.1 g, 6.7 mmol) in MeOH (20 mL) was added NaBH 4 (254 mg, 6.7 mol) at 0 o C. The reaction mixture was stirred at r.t. for 1 h, which was then quenched by water, diluted by DCM, washed by brine, and dried over anhydrous Na 2 SO 4 . The solution was concentrated under reduced pressure and purified by silica gel column chromatography to give Example 39d (2.8 g, yield: 90%) as a white solid. LCMS [M+1-17] + = 448.2. 1 H NMR (400 MHz, Chloroform-d) d 9.26 (s, 1H), 8.50 (s, 1H), 7.62 (t, 2H), 7.30 (d, 1H), 7.02 (s, 1H), 6.78 (s, 1H), 4.92 (s, 2H), 4.77 (s, 1H), 4.49 (q, 2H), 3.44 (s, 3H), 1.46 (d, 3H), 1.41 (s, 9H).
Step 3: Example 39f
[00515] To a solution of Example 39d (2.70 g, 5.8 mmol), Example 39e (2.52 g, 17.5 mmol) in DCM (20 mL) was added DBU (2.64 g, 17.5 mmol) at 0 o C. The reaction mixture was warmed to room temperature and stirred for 3 h. The mixture was concentrated under reduced pressure and purified by silica gel column chromatography to give Example 39f (2.3 g, yield: 65%) as a brown solid.
Step 4: Example 39h
[00516] To a solution of Example 39f (2.30 g, 3.79 mmol), Example 39g (795 mg, 4.54 mmol) in DCM (20 mL) was added CF 3 SO 3 H (285 mg, 1.9 mmol) in DCM (20 mL) at 0 o C. The reaction mixture was warmed to 27 o C and stirred for overnight. The mixture was concentrated under reduced pressure and was purified by C-18 gel column chromatography to give Example 39h (200 mg, yield: 10%) as a brown solid. LCMS [M+1] + = 523.2.
Step 5: Example 39i
[00517] To a solution of Example 39h (200 mg, 0.38 mmol) in MeOH (5 mL) was added NaOH (156 mg, 3.9 mmol) in H 2 O (5 mL), which was stirred at 50 o C for overnight. The mixture was concentrated to give the crude product Example 39i (700 mg crude, yield 100%) as a white solid.
Step 6: Example 39j
[ 00518] To a solution of Example 39i (700 mg crude, 0.38 mmol) in con. HCl (5 mL) was stirred at r.t. for 2 h. The residue was purified by reverse phase column to afford the desired product Example 39j (105 mg, two steps’ yield: 75%) as a brown solid.
LCMS [M+1] + = 395.2.
Step 7: Example 39
[00519] To a solution of Example 39i (100 mg, 0.254 mmol), TEA (51 mg, 0.51 mmol) in DCM (10 mL) was added HATU (144 mg, 0.38 mmol). The reaction mixture was stirred at 25 o C for 2 h. Then DCM (20 mL) was added to the reaction mixture, which was washed with brine (20 mL*2), dried over anhydrous Na 2 SO 4 and concentrated. The residue was purified by Prep-HPLC to afford the desired product Example 39 (4.1 mg, yield 4%) as a white solid. LCMS [M+1] + = 377.1. 1 H NMR (400 MHz, DMSO-d 6 ) d 9.07 (s, 1H), 8.50 (d, 1H), 8.35 (s, 1H), 8.24 (dd, 2H), 7.60 (d, 1H), 7.03 (d, 1H), 6.80 (d, 1H), 6.61 (s, 1H), 4.85 (d, 1H), 4.60 (d, 1H), 4.04 (d, 1H), 3.63 (d, 1H), 3.54 (t, 1H), 3.30 (s, 1H), 3.09 (d, 3H), 1.15 (d, 3H).
Example 40:
Step 1: Example 40b
[00520] To a solution of Example 40a (10.0 g, 51.3 mmol, 1.0 eq) in CCl4 (100 mL) was added NBS (10.04 g, 56.4 mmol, 1.1 eq) and BPO (1.24 g, 5.13 mmol, 0.1 eq) at room temperature. The mixture was stirred for 2 h at 80°C. After the reaction was completed, the mixture was cooled to room temperature. The suspension was diluted with EtOAc (150 mL), which was filtered through a pad of Celite and the filter cake was washed with EtOAc (150 mL). The filtrate was concentrated in vacuum, and the crude was purified by silica gel flash column chromatography to afford the product Example 40b (12.5 g, 88% yield) as a yellow solid. 1 H NMR (300 MHz, DMSO-d 6 ) d 8.00 (d, 1H), 7.95 (d, 1H), 7.66 (dd, 1H), 4.75 (s, 2H).
Step 2: Example 40d
[00521] A solution of Example 40c (3.53 g, 20.15 mmol, 1.1 eq) in THF (50 mL) was cooled to 0 o C, and NaH (1.47 g, 60% in mineral oil, 36.64 mmol, 2.0 eq) was added in portions. The mixture was stirred for 30 min at 0°C, then Example 40b (5.0 g, 18.32 mmol, 1.0 eq) was added at 0 o C, which was stirred for 1 h at room temperature. The mixture was quenched with NH 4 Cl aqueous solution (100 mL) and extracted with EtOAc (100 mL*3). The combined organic layers were washed with brine, dried over Na 2 SO 4 and concentrated vacuum. The crude product was purified by silica gel flash column
chromatography to afford the product Example 40d (3.3 g, 50% yield) as an off-white solid. LCMS [M+1-100] + = 269.1.
Step 3: Example 40e [00522] To a solution of Example 40d (2.0 g, 5.43 mmol, 1.0 eq) in dry dioxane (20 mL) were added Cs 2 CO 3 (5.30 g, 16.3 mmol, 3.0 eq), NH 2 -Boc (700 mg, 5.98 mmol, 1.1 eq), Xantphos (628 mg, 1.1 mmol, 0.2 eq) and Pd 2 (dba) 3 .CHCl 3 (560 mg, 0.543 mmol, 0.1 eq). The reaction mixture was stirred for 2 h at 110 o C under N 2 protection. After the reaction was completed, the solvent was removed and the residue was purified by silica gel flash column chromatography to afford the product Example 40e (2.0 g, 91% yield) as a yellow solid. LCMS [M+23] + = 428.3.
Step 4: Example 40f
[00523] A solution of Example 40e (1.7 g, 4.2 mmol, 1.0 eq) in HCl/dioxane (4M, 20 mL) was stirred for 4 h at room temperature. After the reaction was completed, the mixture was concentrated in vacuum to afford the product Example 40f (860 mg, crude, 100% yield) as a yellow solid. LCMS [M+1] + = 206.3.
Step 5: Example 40h
[00524] To a solution of Example 40g (830 mg, 2.54 mmol, 0.8 eq) in DCM (8 mL) were added DIEA (1.65 g, 12.68 mmol, 4.0 eq) and HATU (964 mg, 2.54 mmol, 0.8 eq). The mixture was stirred for 20 min at room temperature. Then Example 40f (650 mg, 3.17 mmol, 1.0 eq) was added and the mixture was stirred for 2 h at room temperature. After the reaction was completed, it was concentrated in vacuum. The residue was purified by silica gel flash column chromatography to afford the product Example 40h (150 mg, 9% yield) as a yellow solid. LCMS [M+1] + = 514.2.
Step 6:Example 40i
[00525] To a solution of Example 40h (200 mg, 0.39 mmol, 1.0 eq) in dry dioxane (20 mL) were added Cs 2 CO 3 (253.4 mg, 0.78 mmol, 2.0 eq), BINAP (48.5 mg, 0.078 mmol, 0.2 eq) and Pd2(dba)3 (40.4 mg, 0.039 mmol, 0.1 eq). The reaction mixture was stirred for 4 h at 80 o C under N2 protection. After the reaction was completed, the solvent was removed, and the residue was purified by Prep-TLC to afford the product Example 40i (110 mg, 59% yield) as an off-white solid. LCMS [M+1] + = 478.3.
Step 7: Example 40
[00526] To a solution of Example 40i (90 mg, 0.189 mmol, 1.0 eq) in DCM (2 mL) was added HCl/dioxane (1 mL, 4M in dioxane). The reaction was stirred at room temperature for 2 h. After the reaction was completed, the reaction was basified with NaHCO 3 (excess). The solid was filtered out, and the filtrate was concentrated. The crude product was purified by Prep-TLC to afford the product
Example 40 (52.0 mg, 73% yield) as an off-white solid. LCMS [M+1] + = 378.1. 1 H NMR (300 MHz, DMSO-d6) d 9.78 (s, 1H), 8.60 (s, 1H), 8.18 (s, 1H), 8.11 (d, 1H), 8.06 (d, 1H), 7.76 (d, 1H), 7.12 (dd, 1H), 5.97 (s, 1H), 4.70 (d, 1H), 4.53 (d, 1H), 3.94-3.83 (m, 1H), 3.53 (dd, 1H), 3.28-3.20 (m, 1H), 2.96 (d, 3H), 1.15 (d, 3H).
Example 41:
Step 1: Example 41b
[00527] To a solution of Example 41a (10.0 g, 43.7 mmol, 1.0 eq) in CCl 4 (100 mL) were added NBS (11.66 g, 65.5 mmol, 1.5 eq) and AIBN (1.4 g, 8.7 mmol, 0.2 eq) at room temperature. The mixture was stirred for 2 h at 80°C. After the reaction was completed, the solid was filtered, and the filtrate was concentrated. The residue was purified by silica gel flash column chromatography to afford the product Example 41b (11.8 g, 88% yield) as a yellow solid.
Step 2: Example 41d
[00528] To a solution of Example 41c (3.13 g, 17.86 mmol, 1.1 eq) in THF (50 mL) was added NaH (1.30 g, 32.47 mmol, 2.0 eq) in portions at 0 o C. After stirring for 20 min, Example 41b (5.0 g, 16.23 mmol, 1.0 eq) was added at 0 o C. The mixture was stirred for 1 h at room temperature under N 2 . The mixture was quenched with NH 4 Cl aqueous solution (100 mL), which was extracted with EtOAc (200 mL*3). The combined organic layers were washed brine, dried over Na 2 SO 4 and concentrated. The crude product was purified by silica gel flash column chromatography to afford the product Example 41d (1.4 g, 22% yield) as an off-white solid. LCMS [M+1-100] + = 302.1.
Step 3: Example 41e
[00529] To a solution of Example 41d (1.4 g, 3.5 mmol, 1.0 eq) in dioxane (20 mL) were added Cs 2 CO 3 (3.4 g, 10.5 mmol, 3.0 eq), NH 2 -Boc (450 mg, 3.84 mmol, 1.1 eq), Xantphos (404 mg, 0.7 mmol, 0.2 eq) and Pd2(dba)3 (362 mg, 0.35 mmol, 0.1 eq). The reaction mixture was degassed with nitrogen for 3 times and stirred at 110 o C for 2 h. The reaction was cooled to room temperature and concentrated in vacuum. The crude product was purified by silica gel flash column chromatography to afford the product Example 41e (1.42 g, 93% yield) as a yellow solid. LCMS [M+1+22] + = 461.2
Step 4: Example 41f
[00530] A solution of Example 41e (1.42 g, 3.24 mmol, 1.0 eq) in HCl/dioxane (4M, 20 mL) was stirred for 3 h at room temperature. After the reaction was completed, the mixture was concentrated in vacuum to afford the product Example 41f (800 mg, crude, 100% yield) as a yellow solid. LCMS
[M+1] + = 239.2.
Step 5: Example 41h
[00531] To a solution of Example 41g (789 mg, 2.42 mmol, 0.8 eq) in DCM (20 mL) were added DIEA (1.57 g, 12.1 mmol, 4.0 eq) and HATU (1.72 g, 4.51 mmol, 1.5 eq). After stirring for 15 min, Example 41f (720 mg, 3.03 mmol, 1.0 eq) was added. The reaction mixture was stirred for 2 h at room temperature. After the reaction was completed, it was concentrated in vacuum. The residue was purified by silica gel flash column chromatography to afford the product Example 41h (520 mg, 39%yield) as a yellow solid. LCMS [M+1] + = 547.3.
Step 6:Example 41i
[00532] To a solution of Example 41h (440 mg, 0.81 mmol, 1.0 eq) in dioxane (20 mL) were added Cs 2 CO 3 (523 mg, 1.61 mmol, 2.0 eq), BINAP (100 mg, 0.16 mmol, 0.2 eq) and Pd 2 (dba) 3 (83.3 mg, 0.08 mmol, 0.1 eq). The reaction mixture was stirred for 2 h at 80 o C under N 2 protection. The reaction mixture was cooled to room temperature, filtered and concentrated in vacuum. The crude product was purified by Prep-TLC to afford the product Example 41i (220 mg, 54% yield) as an off-white solid. LCMS [M+1] + = 511.2.
Step 7: Example 41j
[00533] To a solution of Example 41i (200 mg, 0.39 mmol, 1.0 eq) in MeOH (4 mL) was added 2 M NaOH aqueous solution (0.4 mL). The reaction mixture was stirred for 1 h at room temperature. After the reaction was completed, the mixture was acidified with HCl aqueous solution (1M), which was then extracted with EtOAc (20 mL*3). The combined organic layers were washed with brine and concentrated in vacuum to afford the product Example 41j (190 mg, 98%yield) as an off-white solid. LCMS [M+1] + = 497.2.
Step 8: Example 41j
[00534] To a solution of Example 41j (170 mg, 0.34 mmol, 1.0 eq) in DCM (8 mL) were added DIEA (178 mg, 1.37 mmol, 4.0 eq) and HATU (195 mg, 0.51 mmol, 1.5 eq). After stirring for 15 min, methylamine hydrochloride (46 mg, 0.69 mmol, 2.0 eq) was added to the reaction mixture, which was stirred for 2 h at room temperature. After the reaction was completed, it was concentrated in vacuum. The residue was purified by Prep-TLC to afford the product Example 41k (170 mg, 97% yield) as an off- white solid. LCMS [M+1] + = 510.2.
Step 9: Example 41
[00535] To a solution of Example 41k (100 mg, 0.196 mmol, 1.0 eq) in DCM (2 mL) was added HCl/dioxane (2 mL, 4M in dioxane) at room temperature. The reaction was stirred at room temperature for 1 h. After the reaction was completed, the reaction was basified with NaHCO 3 (excess). The solid was filtered out, and the filtrate was concentrated. The crude product was purified by Prep-TLC to afford the desired product Example 41 (46.7 mg, 58% yield) as a white solid. LCMS [M+1] + = 410.2 1 H NMR (300 MHz, DMSO-d 6 ) d 10.95 (s, 1H), 8.81 (d, 1H), 8.69 (d, 1H), 8.30 (d, 1H), 8.17 (s, 1H), 7.95 (d, 1H), 7.69 (d, 1H), 7.00-6.90 (m, 1H), 5.74 (s, 1H), 4.65 (d, 1H), 4.54 (d, 1H), 4.00-3.88 (m,1H), 3 .61-3.54 (m, 1H), 3.44-3.37 (m, 1H), 2.97 (d, 3H), 2.83 (d, 3H), 1.18 (d, 3H).
Example 42:
Step 1: Example 42b
[00536] To a solution of Example 42a (10.0 g, 38.7 mmol, 1.0 eq) in dry THF (193 mL) was added n- BuLi (17 mL, 2.5 M in hexane, 42.6 mmol, 1.1 eq) dropwise at -78°C over a period of 15 min, followed by stirring for 20 min. DMF (28.3 g, 387 mmol, 10.0 eq) was added dropwise to the mixture at -78°C and the resulting mixture was stirred at -78°C for another 1 h under N2. The reaction mixture was quenched with 1N NH 4 Cl aqueous solution (100 mL), which was stirred for 30 min at 0°C. The reaction mixture was then extracted with EtOAc (100 mL*3). The combined organic layers were washed with brine, dried over Na 2 SO 4 , and concentrated. The residue was purified by silica column chromatography (Petroleum Ether) to afford the desired product Example 42b (6.1 g, 71% yield) as yellow oil.
1H NMR (300 MHz, CDCl 3 -d) d 10.06 (s, 1H), 8.01 (s, 1H), 7.91-7.85 (m, 2H).
Step 2: Example 42c
[00537] To a solution of Example 42b (4.9 g, 23.6 mmol, 1.0 eq) in ethanol (100 mL) was added NaBH 4 (985 mg, 25.9 mmol, 1.1 eq) in portions at 0 o C. The reaction was stirred for 1 h at room temperature. The reaction mixture was quenched with 1N NH 4 Cl aqueous solution (100 mL) and stirred for 30 min at 0°C. The reaction mixture was extracted with EtOAc (100 mL*3). The combined organic layers were washed with brine, dried over Na 2 SO 4 and concentrated. The residue was purified by silica column chromatography (Petroleum Ether) to afford the desired product Example 42c (3.7 g, 75% yield) as a yellow oil.
Step 3:Example 42d [00538] To a solution of Example 42c (3.7 g, 17.6 mmol, 1.0 eq) in DCM (50 mL) was added PBr 3 (5.2 g, 19.4 mmol, 1.1 eq) at 0 o C. The reaction was stirred for 2 h at 0 o C. The reaction mixture was diluted with H 2 O (40 mL), and extracted with EtOAc (40 mL*3). The combined organic layers were washed with NaHCO 3 aqueous solution, dried over Na 2 SO 4 and concentrated. The residue was purified by silica column chromatography (Petroleum ether) to afford the desired product Example 42d (2.1 g, 44% yield) as yellow oil.
Step 4: Example 42f
[00539] Toa solution of Example 42e (1.5 g, 8.49 mmol, 1.1 eq) in THF (50 mL) was added NaH (864 mg, 60% in mineral oil, 21.6 mmol, 2.8 eq) in portions at 0 o C. After stirring for 15 min, Example 42d (2.1 g, 7.72 mmol, 1.0 eq) was added at 0 o C. The mixture was stirred for 2 h at room temperature. The reaction was quenched with H 2 O (50 mL) and extracted with EtOAc (50 mL*3). The combined organic layers were washed with brine, dried over Na 2 SO 4 and concentrated. The residue was purified by silica gel chromatography to afford the desired product Example 42f (850 mg, 27% yield) as a white solid. LCMS [M+1-100] + = 268.0.
Step 5: Example 42h
[00540] To a solution of Example 42f (854 mg, 2.3 mmol, 1.0 eq) and Example 42g (523 mg, 3.0 mmol, 1.3 eq) in dioxane (20 mL) were added 3G-Brettphos-Pd (211 mg, 0.23 mmol, 0.1 eq) and Cs 2 CO 3 (1.13 g, 3.54 mmol, 1.5 eq). The mixture reaction was degassed with nitrogen for 3 times and stirred at 100 o C for 16 h. The reaction was cooled to room temperature and concentrated. The residue was purified by silica gel chromatography to afford the desired product Example 42h (720 mg, 70% yield) as a yellow solid. LCMS [M+1-100] + = 349.2.
Step 6: Example 42i
[00541] To a solution of Example 42h (720 mg, 1.6 mmol, 1.0 eq) in DCM (10 mL) was added HCl/dioxane (8 mL, 4M in dioxane, 32 mmol, 20.0 eq). The mixture was stirred at room temperature for 4 h. After the reaction was completed, the solvent was concentrated to give Example 42i (700 mg, crude) as a yellow solid. The crude was used next step directly without further purification. LCMS [M+1] + = 249.2.
Step 7: Example 42k
[00542] To a solution of Example 42j (791 mg, 2.42 mmol, 1.0 eq) in DCM (15 mL) were added DIEA (780 mg, 6.05 mmol, 2.5 eq) and HATU (1.01 g, 2.66 mmol, 1.1 eq). After stirring for 15 min, Example 42i (600 mg, 2.42 mmol, 1.0 eq) was added to the mixture. The reaction solution was stirred for 6 h at room temperature. After the reaction was completed, the solvent was removed and the crude was purified by silica gel chromatography to afford the desired product Example 42k (421 mg, 31% yield) as a yellow solid. LCMS [M+1] + = 557.3.
Step 8: Example 42m
[00543] To a solution of Example 42k (200 mg, 0.36 mmol, 1.0 eq) in dioxane (5 mL) were added Cs 2 CO 3 (234 mg, 0.72 mmol, 2.0 eq), 3G-Brettphos-Pd (33.0 mg, 0.036 mmol, 0.1 eq). The reaction mixture was stirred for 16 h at 100 o C under N 2 . The reaction mixture was cooled to room temperature, filtered and concentrated in vacuum. The crude product was purified by prep-TLC to afford the desired p roduct Example 42m (110 mg, 59% yield) as light yellow oil. LCMS [M+1]+ = 521.1.
Step 9:Example 42
[00544] To a solution of Example 42m (100 mg, 3.1 mmol) in DCM (2 mL) was added HCl/dioxane (1 mL, 4M in dioxane) at room temperature. The reaction was stirred at room temperature for 4 h. After completed, the reaction was basified with NaHCO 3 . The solid was filtered out, and the filtrate was concentrated. The crude product was purified by Prep-TLC (EtOAc) to afford the desired product
Example 42 (42.2 mg, 53% yield) as an off-white solid. LCMS [M+1] + = 421.1. 1 H NMR (300 MHz, DMSO-d 6 ) d 8.81 (s, 1H), 8.42 (s, 1H), 8.15 (s, 1H), 8.00-7.96 (m, 2H), 7.69 (d, 1H), 7.22 (d, 1H), 6.08 (s, 1H), 4.67 (d, 1H), 4.49 (d, 1H), 3.86-3.84 (m, 1H), 3.49-3.45 (m, 1H), 3.21-3.15 (m, 1H), 2.95 (d, 3H), 1.14 (d, 3H).
Example 43:
Step 1: Example 43b
[00545] To a solution of Example 43a (2.0 g, 7.84 mmol, 1.0 eq) in CCl 4 (40 mL) were added NBS (1.54 g, 8.63 mmol, 1.10 eq), AIBN (129 mg, 0.78 mmol, 0.1 eq). The reaction was stirred for 3 h at 80 o C under N 2 . The mixture was cooled to room temperature. The suspension was diluted with EtOAc (50 mL), and then filtered through a pad of Celite. The filter cake was washed with EtOAc (50 mL). The filtrate was concentrated in vacuum, and the crude product was purified by silica column
chromatography (Petroleum Ether) to afford the desired product Example 43b (2.2 g, 84% yield) as yellow oil. 1 H NMR (300 MHz, CDCl 3 -d) d 7.86 (d, 1H), 7.68 (s, 1H), 7.57 (d, 1H), 4.42 (s, 2H).
Step 2: Example 43d
[00546] To a solution of Example 43c (1.3 g, 7.29 mmol, 1.1 eq) in THF (50 mL) was added NaH (742 mg, 60% in mineral oil, 18.6 mmol, 2.8 eq) in portions at 0 o C. After stirring for 15 min, Example 43b (2.2 g, 6.62 mmol, 1.0 eq) was added to the mixture at 0 o C, which was stirred for 2 h at room temperature under N 2 . The reaction was quenched with H 2 O (50 mL) and extracted with EtOAc (50 mL*3). The combined organic layers were washed with brine, dried over Na 2 SO 4 and concentrated in vacuum. The crude product was purified by silica gel chromatography to afford the desired product Example 43d (1.3 g, 41% yield) as yellow oil. LCMS [M+1-100] + = 328.0.
Step 3: Example 43f
[00547] To a solution of Example 43d (800 mg, 1.87 mmol, 1.0 eq) and Example 43e (328 mg, 2.8 mmol, 1.5 eq) in dioxane (10 mL) were added Pd 2 (dba) 3 (171 mg, 0.187 mmol, 0.1 eq), Xantphos (324 mg, 0.561 mmol, 0.3 eq) and Cs 2 CO 3 (1.22 g, 3.74 mmol, 2.0 eq). The reaction mixture was degassed with nitrogen for 3 times and stirred at 100 o C for 6 h. The reaction was cooled to room temperature and concentrated in vacuum. The crude product was purified by silica gel chromatography to afford the desired crude product Example 43f (612 mg, 70% yield) as a yellow solid. LCMS [M+1-100] + = 365.2. Step 4: Example 43g
[00548] To a solution of Example 43f (612 mg, 1.31 mmol, 1.0 eq) in DCM (5 mL) was added HCl/dioxane (7 mL, 4M in dioxane, 26.2 mmol, 20.0 eq). The mixture was stirred at room temperature for 4 h. After the reaction was completed, the solvent was concentrated to give Example 43g (520 mg, crude) as a white solid. LCMS [M+1] + = 265.2.
Step 5: Example 43i
[00549] To a solution of Example 43h (572 mg, 1.75 mmol, 1.1 eq) in DCM (20 mL) were added DIEA (513 mg, 3.98 mmol, 2.5 eq) and HATU (725 mg, 1.91 mmol, 1.2 eq). After stirring for 15 min, Example 43g (420 mg, 1.59 mmol, 1.0 eq) was added to the mixture, which was stirred for 6 h at room temperature. After the reaction was completed, the solvent was removed and the crude product was purified by Prep-TLC to afford the desired product Example 43i (198 mg, 22% yield) as a yellow solid. LCMS [M+1] + = 573.1.
Step 6: Example 43j
[00550] To a solution of Example 43i (100 mg, 0.175 mmol, 1.0 eq) in dioxane (10 mL) were added Cs 2 CO 3 (114 mg, 0.35 mmol, 2.0 eq) and 3rd-t-Bu-Xphos-Pd (27 mg, 0.035 mmol, 0.1 eq). The reaction mixture was stirred for 6 h at 100 o C under N2. The reaction mixture was cooled to room temperature, filtered and concentrated in vacuum. The crude product was purified by Prep-TLC to afford the desired product Example 43j (53 mg, 56% yield) as a light yellow solid. LCMS [M+1] + = 537.4.
Step 7:Example 43
[00551] To a solution of Example 43j (78 mg, 0.145 mmol, 1.0 eq) in DCM (1 mL) was added HCl/dioxane (0.7 mL, 4M in dioxane, 2.91 mmol, 20.0 eq) at room temperature. The reaction was stirred at room temperature for 4 h. After the reaction was completed, the reaction was basified with NaHCO 3 (excess). The solid was filtered out, and the filtrate was concentrated. The crude product was purified by prep-TLC (EtOAc) to afford the desired product Example 43 (33.2 mg, 52% yield) as an off-white solid. LCMS [M+1] + = 437.1. 1 H NMR (300 MHz, DMSO-d 6 ) d 9.32 (s, 1H), 8.64 (d, 1H), 8.17 (s, 1H), 8.15 (d, 1H), 7.97 (d, 1H), 7.37 (dd, 1H), 7.03 (dd, 1H), 5.99 (s, 1H), 4.62 (d, 1H), 4.49 (d, 1H), 3.92-3.90 (m, 1H), 3.52 (dd, 1H), 3.35-3.30 (m, 1H), 2.95 (d, 1H), 1.16 (d, 1H).
Example 44:
Step 1: Example 44b
[00552] To a solution of Example 44a (11.0 g, 56.1 mmol, 1.0 eq) in CCl4 (500 mL) were added NBS (15.0 g, 84.2 mmol, 1.5 eq) and AIBN (4.6 g, 28.1 mmol, 0.5 eq). The reaction mixture was stirred at 80°C for 8 h. After the insoluble solid was removed, the filtrate was concentrated, and the residue was purified by silica gel flash column chromatography to afford the product Example 44b (8.1 g, 53% yield) as a yellow solid. LCMS[M+1] + = 275.9.
Step 2: Example 44d
[00553] To a solution of Example 44c (2.9 g, 16.4 mmol, 1.5 eq) in THF (40 mL) was added NaH (567 mg, 60% in mineral oil, 14.2 mmol, 1.3 eq) in portions at 0 o C. After stirring for 10 min, a solution of Example 44b (3.0 g, 10.9 mmol, 1.0 eq) in THF (10 mL) was added dropwise. The reaction mixture was stirred for 2 h at 0°C~r.t. and then the solvent was concentrated in vacuo. The crude product was purified by silica gel flash column chromatography to afford the desired product Example 44d (1.9 g, 47% yield) as a yellow solid. LCMS [M+1] + = 369.3.
Step 3: Example 44f
[00554] To a solution of Example 44d (1.8 g, 4.9 mmol, 1.0 eq) in dioxane (50 mL) were added Cs 2 CO 3 (3.2 g, 9.8 mmol, 2.0 eq) and 3rd-Brettphos-Pd (442 mg, 0.5 mmol, 0.1 eq). The reaction mixture was stirred at 110°C for 5 h under N2. After cooled to room temperature, the solvent was removed, and the residue was purified by silica gel flash column chromatography to afford the product Example 44f (1.4 g, 71% yield) as yellow oil. LCMS [M+1] + = 406.2.
Step 4: Example 44g A solution of Example 44f (700 mg, 1.7 mmol, 1.0 eq) in HCl/dioxane (15 mL, 4M in dioxane) was stirred for 4 h at 40°C. The reaction mixture was concentrated in vacuo to afford the desired product E xample 44g (640 mg, crude) as a white solid. LCMS [M+1]+ = 206.2.
Step 5: Example 44i
[00555] To a solution of Example 44h (465 mg, 1.4 mmol, 1.0 eq) in DCM (20 mL) were added DIEA (1.8 g, 14.2 mmol, 10.0 eq), HATU (649 mg, 1.7 mmol, 1.2 eq) and Example 44g (620 mg, 2.6 mmol, 1.8 eq). The reaction mixture was stirred for 2 h at r.t. The solvent was removed, and the residue was purified by silica gel flash column chromatography to afford the desired product Example 44i (185 mg, 25% yield) as yellow oil. LCMS [M+1] + = 514.3.
Step 6: Example 44j
[00556] To a solution of Example 44i (180 mg, 0.35 mmol, 1.0 eq) in dioxane (50 mL) were added Cs 2 CO 3 (228 mg, 0.7 mmol, 2.0 eq) and 3rd-t-Bu-Xphos-Pd (93 mg, 0.11 mmol, 0.3 eq). The reaction mixture was stirred at 100°C for 5 h under N 2 . After cooled to room temperature, the solvent was removed, and the residue was purified by silica gel flash column chromatography to afford the product Example 44j (95 mg, 57% yield) as a yellow solid. LCMS [M+1] + = 478.2.
Step 7: Example 44
[00557] To a solution of Example 44j (90 mg, 0.19 mmol, 1.0 eq) in DCM (2 mL) was added
HCl/dioxane (1 mL, 4M in dioxane). The reaction mixture was stirred at r.t. for 4 h and then concentrated in vacuum. The residue was dissolved in MeOH (5 mL), and basified with NaHCO 3 . After concentration, the residue was purified by prep-TLC to afford the desired product Example 44 (41.5 mg, 58% yield) as an off-white solid. LCMS [M+1] + = 378.2. 1 H NMR (300 MHz, DMSO-d6) d 9.96 (s, 1H), 8.90 (s, 1H), 8.19-8.18 (m, 2H), 8.03 (d, 1H), 7.36 (s, 1H), 7.30 (s, 1H), 5.52 (s, 1H), 4.65 (d, 1H), 4.55 (d, 1H), 3.95- 3.93 (m, 1H), 3.59 - 3.55 (m, 1H), 3.42- 3.37 (m, 1H), 2.95 (d, 3H), 1.18 (d, 3H).
Example 45:
Step1: Example 45b
[ 00558] To a solution of Example 45a (5.0 g, 24.9 mmol, 1.0 eq) in CCl 4 (50 mL) were added NBS (4.9 g, 27.4 mmol, 1.1 eq) and AIBN (410 mg, 2.5 mmol, 0.1 eq). The reaction mixture was stirred at 80°C for 6 h. After cooled to room temperature, the solvent was removed, and the residue was purified by silica gel flash column chromatography (Petroleum Ether) to afford the product Example 45b (4.5 g, 60% yield) as a white solid. LCMS [M+1] + = 281.2.
Step 2: Example 45d
[00559] To a solution of Example 45c (1.9 g, 10.7 mmol, 1.5 eq) in DMF (20 mL) was added NaH (340 mg, 60% in mineral oil, 8.5 mmol, 1.2 eq) in portions at 0°C. The mixture was stirred for 30 min at the same temperature, and then Example 45b (2.0 g,7.1 mmol, 1.0 eq) in DMF (20 mL) was added dropwise. The reaction mixture was stirred at r.t. for 2 h. The mixture was poured into saturated aqueous solution of NH 4 Cl (50 mL), which was then extracted with EtOAc (70 mL*3). The combined organic layers were washed with brine, dried over Na 2 SO 4 , and concentrated. The crude product was purified by silica gel flash column chromatography to afford the product Example 45d (1.3 g, 50% yield) as a white solid. LCMS [M+1] + = 374.3.
Step 3: Example 45e
[00560] To a solution of Example 45d (1.3 g, 3.5 mmol, 1.0 eq) in dioxane (15 mL) were added Cs 2 CO 3 (2.3 g, 7.0 mmol, 2.0 eq), NH 2 -Boc (1.2 g, 10.5 mmol, 3.0 eq), BINAP (436.1 mg, 0.7 mmol, 0.2 eq) and Pd2(dba)3.CHCl 3 (362.3 mg, 0.35 mmol, 0.1 eq). The reaction mixture was stirred for 2 h at 110 o C under N2 protection. After cooled to room temperature, the solvent was removed. The crude product was purified by silica gel flash column chromatography to afford the product Example 45e (980 mg, 68% yield) as a yellow solid. LCMS [M+1] + = 411.3.
Step 4: Example 45f
[00561] To a solution of Example 45e (980 mg, 2.4 mmol, 1.0 eq) in DCM (5 mL) was added TFA (2.5 mL) dropwise at 0 o C. The reaction mixture was stirred for 2 h at r.t. The solution was concentrated in vacuum to give the crude product Example 45f (1.6 g, crude, quant) as yellow oil, which was used to next step directly without purification. LCMS [M+1] + = 211.3.
Step 5: Example 45h
[00562] To a solution of Example 45g (392 mg, 1.2 mmol, 0.5 eq) in DCM (8 mL) were added HATU (1.0 g, 2.8 mmol, 1.2 eq) and DIEA (1.2 g, 9.2 mmol, 4.0 eq). The mixture was stirred for 20 min, then Example 45f (700 mg, 2.3 mmol, 1.0 eq) was added. The reaction mixture was stirred at r.t. for 2 h. The solution was concentrated in vacuum, and the crude product was purified by silica gel flash column chromatography to afford the product Example 45h (280 mg, 23% yield) as a yellow solid. LCMS [M+1] + = 519.4.
Step 6: Example45i
[00563] To a solution of Example 45h (260 mg, 0.5 mmol, 1.0 eq) in dioxane (3 mL) were added Cs 2 CO 3 (326 mg,1.0 mmol, 2.0 eq) and 3 rd t-Bu-Xphos-Pd (44.1 mg, 0.05 mmol, 0.1 eq). The reaction mixture was stirred for 3 h at 80 o C under N 2 protection. The solid was filtered out and filtrate was concentrated. The residue was purified by prep-TLC to afford the Example45i (120 mg, 50% yield) as a y ellow solid. LCMS [M+1] + = 483.3.
Step 7: Example 45
[00564] To a solution of Example 45i (100 mg, 0.20 mmol, 1.0 eq) in DCM (3 mL) was added HCl/dioxane (3 mL, 4M in dioxane) dropwise at 0 o C. The reaction mixture was stirred for 2 h at r.t. After completion, the reaction mixture was concentrated. The crude product was dissolved in MeOH (2 mL), and then NaHCO 3 (excess) was added to the mixture, which was stirred for 20 min at r.t. After DCM (20 mL) was added to the mixture, the solid was filtered out and the filtrate was concentrated. The residue was purified by Prep-TLC to afford the Example 45 (42.8 mg, 54% yield) as an off-white solid. LCMS [M+1] + = 383.2. 1 H NMR (300 MHz, DMSO-d 6 ) d 9.62 (s, 1H), 8.39 (d, 1H), 8.16-8.13 (m, 2H), 7.89 (d, 1H), 6.49 (s, 2H), 5.48(s, 1H), 4.60 (d, 1H), 4.49 (d, 1H), 3.92-3.89 (m, 1H), 3.76 (s, 3H), 3.54-3.50 (m,1H), 3.42-3.36 (m, 1H), 2.93 (d, 3H), 1.18 (d, 3H).
Example 46:
Step 1: Example 46b
[00565] To a solution of Example 46a (5.0 g, 32.3 mmol, 1.0 eq) in CCl 4 (30 mL) was added BPO (2.3 g, 9.7l mmol, 0.3 eq) at 80 o C. After stirring for 5 min, NBS (6.9 g, 38.76 mmol, 1.2 eq) was added, which was stirred for 16 h at 80 o C. After the reaction was completed, EtOAc (150 mL) was added to the suspension, which was washed with saturated NaHCO 3 aqueous (100 mL*3). The organic layer was dried over Na 2 SO 4 and concentrated to afford the crude product Example 46b (4.2 g, 56% yield) as yellow oil. LCMS [M+1] + = 234.1
Step 2: Example 46d
[00566] To a solution of Example 46c (2.7 g, 15.5 mmol, 1.2 eq) in THF (40 mL) was added NaH (770 mg, 60% in mineral oil, 19.4 mmol, 1.5 eq) in portions at 0 o C. After stirring for 10 min, a solution of Example 46b (3.0 g, 12.9 mmol, 1.0 eq) in THF (5 mL) was added dropwise. The reaction mixture was stirred for 2 h at r.t. The reaction was quenched with saturated NH 4 Cl aqueous (50 mL) at 0 o C and extracted with EtOAc (100 mL*3). The combined organic layers were washed with brine（100 mL*2）, dried over Na 2 SO 4 and concentrated in vacuo. The crude product was purified by silica gel flash column chromatography to afford the desired product Example 46d (3.1 g, 73% yield) as yellow oil. LCMS [M+1] + = 329.3.
Step 3: Example 46e
[00567] To a solution of Example 46d (2.5 g, 7.62 mmol, 3.0 eq) in MeOH (30 mL) was added 10% Pd/C (1.0 g) in portions under N2 protection. The mixture was degassed with H 2 three times， which was stirred for 2 h at r.t. under H 2 balloon. The solid was filtered out, and the filtrate was concentrated in vacuo to give the desired product Example 46e (2.8 g, quant) as gray oil. LCMS [M+1] + = 299.3.
Step 4: Example 46f
[00568] To a solution of Example 46e (1.5 g, 5.03 mmol, 1.0 eq) in DCM (25 mL) was added
HCl/dioxane (5 mL, 4 M in dioxane) at 0 o C. The reaction mixture was stirred for 2 h at r.t. The reaction solution was concentrated in vacuo. The crude product was dissolved in MeOH, and Na2CO 3 (excess) was added, which was stirred for 10 min at r.t. The solid was filtered out, and the filtrate was concentrated. The crude was purified by silica gel flash column chromatography to afford the desired product Example 46f (860 mg, 87% yield) as yellow oil. LCMS [M+1] + = 199.2.
Step 5: Example 46h
[00569] To a solution of Example 46g (200 mg, 1.01 mmol, 1.0 eq) and DIEA (521 mg, 4.04 mmol, 4.0 eq) in DCM (5 mL) were added HATU (460 mg, 1.21 mmol, 1.2 eq). After stirring for 10 min,
Example 46f (329 mg, 1.01 mmol, 1.0 eq) was added, which was stirred for 2 h at r.t. The mixture was concentrated in vacuo. The residue was purified by silica gel flash column chromatography to afford the product Example 46h (160 mg, 31%yield) as a yellow solid. LCMS [M+1] + = 507.3.
Step 6: Example 46i
[00570] To a solution of Example 46h (160 mg, 0.32 mmol, 1.0 eq) in dioxane (8 mL) were added Cs 2 CO 3 (308 mg, 0.96 mmol, 3.0 eq) and 3 rd -t-Bu-Xphos-Pd (84 mg, 0.096 mmol, 0.3 eq). The reaction mixture was stirred for 3 h at 80 o C under N 2 . The reaction solution was filtered and the filtrate was concentrated in vacuo. The crude product was purified by prep-TLC to afford the desired product
Example 46i (45 mg, 30% yield) as a yellow solid. LCMS [M+1] + = 471.3.
Step 7: Example 46
[00571] To a solution of Example 46i (66 mg, 0.14 mmol, 1.0 eq) in DCM (4mL) was added
HCl/dioxane (2 mL, 4 mol/L in dioxane) at 0 o C, which was stirred for 2 h at r.t. The reaction solution was concentrated in vacuo. The crude product was dissolved in MeOH, and Na 2 CO 3 (excess) was added to the mixture, which was stirred for 10 min at r.t. The solid was filtered out, and the filtrate was concentrated. The crude was purified by prep-TLC to afford the desired product Example 46 (20 mg, 36% yield) as a yellow solid. LCMS [M+1] + = 371.3. 1 H NMR (300 MHz, DMSO-d 6 ) d 9.79 (s, 1H), 8.46 (s, 1H), 8.31 (d, 1H), 8.16 (s, 1H), 7.93-8.01 (m, 1H), 6.66-6.75 (m, 2H), 5.49 (s, 1H), 4.60 (d, 1H), 4.50 (d, 1H), 3.87-3.90 (m, 1H), 3.55 (dd, 1H), 3.44-3.38 (m,1H), 2.93 (d, 3H), 1.18 (d, 3H).
Example 47:
Step 1: Example 47c
[00572] To a solution of Example 47b (525 mg, 3.0 mmol) in THF (15 mL) was added NaH (172 mg, 60% in mineral oil, 4.5 mmol) at 0 o C. The reaction mixture was warmed to room temperature and stirred for 0.5 h. Then, Example 47a (741 mg, 3.0 mmol) was added. The resulting mixture was stirred at r.t. for 6 h. The mixture was quenched by aq.NH 4 Cl, and then extracted by EtOAc, and dried over anhydrous Na 2 SO 4 . The solution was concentrated under reduced pressure and purified by silica gel column chromatography to give Example 47c (200 mg, yield: 20%) as a yellow solid. LCMS [M-174] + = 167.0. Step 2: Example 47d
[00573] A mixture of Example 47c (200 mg, 0.58 mmol) and 10% Pd/C (30 mg) in MeOH (5 mL) was stirred at r.t. for 2 h under 1 atm H 2 . The mixture was then filtered, and the filtrate was concentrated under reduced pressure to give crude Example 47d (200 mg crude, yield: ~100%) as a yellow solid, which was used in the next step directly. LCMS [M-174] + = 137.1
Step 3: Example 47e
[00574] To a solution of Example 47d (170 mg, 0.55 mmol) in DCM (5.0 mL) was added TFA (1.0 mL), which was stirred at r.t. for 2 h. The mixture was concentrated to give the crude product Example 168g (403.5 mg crude, yield: ~100%) as black oil.
Step 4: Example 47g
[00575] To a solution of Example 47e (403 mg crude, 0.61 mmol), Example 47f (197 mg, 0.61 mmol), TEA (900 mg, 9.0 mmol) in DCM (10 mL) was added HATU (230 mg, 0.605 mmol). The reaction mixture was stirred at r.t. for 2 h. Then DCM (40 mL) was added to the reaction mixture, which was washed with brine (20 mL*2), dried over anhydrous Na 2 SO 4 and concentrated. The residue was purified by silica gel column chromatography to afford the desired product Example 47g (200 mg, yield: 64%) as a brown solid. LCMS [M+1] + = 520.2
Step 5: Example 47h
[00576] To a mixture of Example 47g (200 mg, 0.39 mmol), Cs 2 CO 3 (190 mg, 0.59 mmol) in dioxane (10.0 mL) was added 3rd-t-Bu-Xphos-Pd (35 mg, 0.039 mmol). The mixture was degassed with N2 three times, and stirred for 3 h at 80°C. Then the reaction mixture diluted by EtOAc, washed by water, dried over anhydrous Na 2 SO 4 , and then concentrated under reduced pressure to afford crude Example 47h (240 mg crude, yield: ~100%) as a white solid, which was used in the next step without further purification. LCMS [M+1] + = 484.2
Step 6: Example 47
[00577] To a solution of Example 47h (240 mg crude, 0.49 mmol) in THF (1.4 mL) was added HCl/MeOH (2.0 mL, 6.0 moL/L), which was stirred at r.t. for 2 h. The mixture was concentrated, and the residue was purified by Prep-HPLC to afford the desired product Example 47 (13.3 mg, yield: 7% over 2 steps) as a white solid. LCMS [M+1] + = 384.2. 1 H NMR (400 MHz, DMSO-d 6 ) d 9.13 (s, 1H), 8.57 (d, 1H), 8.11 (s, 1H), 8.05 (d, 1H), 7.90 (d, 1H), 7.71 (d, 1H), 5.90 (s, 1H), 4.54 (d, 1H), 4.43 (d, 1H), 3.95 (s, 3H), 3.86 (t, 1H), 3.46 (dd, 1H), 3.28 (dd, 1H), 2.89 (d, 3H), 1.12 (d, 3H).
Example 48:
Step 1: Example 48b
[00578] To a solution of H 2 SO 4 (200 mL) in H 2 O (620 mL) was added HNO 3 (56 g, 889 mmol) at 0 o C. Then Example 48a (88 g, 471 mmol) was added and the resulting mixture was stirred at r.t. for overnight. After completion, the mixture was extracted by EtOAc, and dried over anhydrous Na 2 SO 4 . The solution was concentrated under reduced pressure, which was purified by silica gel column chromatography to give Example 48b (81 g, yield: 74%) as a yellow solid.
Step 2: Example 48c
[00579] To a stirred solution of Example 48b (20 g, 86.6 mmol), and K 2 CO 3 (23.6 g,171mmol) in DMF (70 mL) was added CH3I (17g, 111.8 mmol) at 25 o C. Then the reaction mixture was stirred at 60 o C for 4 h. The reaction mixture was extracted by EtOAc. The organic layer was washed by brine, and dried over anhydrous Na 2 SO 4 . The solution was concentrated under reduced pressure. The residue was purified by silica gel column chromatography to give Example 48c (22.5 g, yield: quant.) as a yellow solid. Step 3: Example 48d [00580] To a solution of Example 48c (10.0 g, 40.64 mmol) in CCl 4 (10 mL) were added NBS (9.4 g, 52.84 mmol) and BPO (3.94 g, 16.26 mmol), which was stirred at 80 o C for 16 h. The mixture was concentrated under reduced pressure, which was purified by silica gel column chromatography to give Example 48d (7.8 g, yield: 59%) as a yellow solid.
Step 4: Example 48f
[00581] To a solution of NaH (1.0 g, 25.1 mmol) in THF (70 mL) were added Example 48d (6.8 g, 20.9 mmol) and Example 48e (4.4 g, 25.1 mmol) at 0 o C. The mixture was stirred at r.t. for 16 h. The reaction mixture was quenched by H 2 O, then concentrated under reduced pressure, which was purified by silica gel column chromatography to afford the desired product Example 48f (6.25 g, yield: 71%) as a yellow oil. LCMS [M-100+1] + = 319.0/321.0.
Step 5: Example 48g
[00582] To a solution of HCl/MeOH (4M, 70 mL) was added Example 48f (6.25 g, 14.9 mmol). Then the reaction mixture was stirred at 14 o C for 2 h. The mixture was concentrated to give crude Example 48g (5.17 g, crude) as a yellow solid, which was used in the next step without further purification. LCMS [M+1] + = 319.0/321.0
Step 6: Example 48i
[00583] To a stirred solution of Example 48g (5 g crude, 15.67 mmol) in DCM (50 mL) were added HATU (7.68 g, 23.5 mmol), DIEA (4.57 g, 47.0 mmol), and Example 48h (7.68 g, 23.5 mmol). The mixture was stirred at 25 o C for 3 h. Then the reaction mixture was concentrated under reduced pressure, which was purified by silica gel column chromatography to afford the desired product Example 48i (8.4 g, yield: 85%) as a yellow solid. LCMS [M+1] + = 627.1/629.1.
Step 7: Example 48j
[00584] To a solution of Example 48i (1 g, 1.59 mmol) in EtOH (10 mL) was added SnCl2 (0.91 g, 4.78 mmol), which was stirred at 14 o C for 2 h. The mixture was concentrated and purified by silica gel column chromatography to afford the desired product Example 48j (1 g, crude) as a yellow solid. LCMS [M+1] + = 597.1/599.1.
Step 8: Example 48k
[00585] To a solution of Example 232i (700 mg, crude) in THF (30 mL) was added t-BuOK (394 mg, 3.51 mmol) at 0 o C. Then the mixture solution was stirred at 25 o C for 1 h. The mixture was concentrated, and the residue was purified by silica gel column chromatography to afford the desired product Example 48k (450 mg, yield: 69%) as yellow oil. LCMS [M+1] + = 561.1/563.1.
Step 9: Example 48
[00586] To a solution of Example 48k (100 mg, 0.34 mmol) in MeOH (1 mL) was added HCl/MeOH (1.0 mL, 6.0 moL/L), which was stirred at r.t. for 2 h. The mixture was concentrated, and the residue was purified by Prep-HPLC to afford the desired product Example 48 (36.5 mg, yield: 44%) as a white solid. LCMS [M+1] + = 461.1. 1 H NMR (400 MHz, DMSO-d 6 ) d 9.17 (s, 1H), 8.51 (s, 1H), 8.16 (s, 1H), 8.13 (s, 1H), 7.92 (d, 1H), 7.17 (d, 1H), 6.03 (s, 1H), 4.54 (d, 1H), 4.42 (d, 1H), 3.89-3.87 (m, 1H), 3.77 (s, 3H), 3.49 (d, 1H), 3.33 (d, 1H), 2.92 (d, 3H), 1.14 (d, 3H).
Example 49:
Step 1: Example 49c
[00587] To a solution of Example 49a (2.04 g, 10.0 mmol), Example 49b (850 mg, 10.0 mmol), and Cs 2 CO 3 (4.89 g, 15.0 mmol) in dioxane (30 mL) were added Pd2(dba)3 (458 mg, 0.5 mmol), and Xantphos (298 mg, 0.5 mmol). The mixture was degassed with N2 three times, and stirred for 3 h at 70°C. Then the reaction mixture was diluted by EtOAc, washed by water, dried over anhydrous Na 2 SO 4 , and then concentrated under reduced pressure. The residue was purified by silica gel column chromatography to afford the desired product Example 49c (1.9 g, yield: 75%.) as a white solid. LCMS [M+1] + = 255.0 Step 2: Example 49d
[00588] To a solution of Example 49c (1.1 g, 4.33 mmol) in THF (30 mL) were added NaBH4 (165 mg, 4.33 mmol) and LiCl (1.3 g, 34.64 mmol) at 0°C. The mixture was degassed with N2 three times, and stirred for overnight at 20°C. The reaction mixture was then quenched by the addition of water (1.2 mL) at 0°C. The resulting solution was diluted with aqueous NaOH solution (15%, 3.6 mL), followed by EtOAc (1.2 mL) at room temperature. The solid was filtered off. The resulting filtrate was concentrated under reduced pressure, which was purified by silica gel column chromatography to afford the desired product Example 49d (400 mg, yield: 41%) as a yellow solid. LCMS [M+1] + = 227.0
Step 3: Example 49e
[00589] To a solution of Example 49d (400 mg, 1.77 mmol) in DCM (5 mL) was added PPh 3 (696 mg, 2.66 mmol). The mixture was cooled to 0°C, then a solution of CBr 4 (701 mg, 2.12 mmol) in DCM (5 mL) was added drop-wise. After addition, the reaction mixture was stirred at 20°C overnight. Then the solution was concentrated under reduced pressure, and purified by silica gel column chromatography to afford the desired product Example 49e (460 mg, yield: 90%) as a yellow solid. LCMS [M+1] + = 288.9. Step 4: Example 49g
[00590] To a solution of Example 49e (460 mg, 1.59 mmol), and Example 49f (332 mg, 1.89 mmol) in THF (10 mL) were added NaH (87 mg, 60% in mineral oil, 2.18 mmol), and TBAI (60 mg, 0.16 mmol) at 0°C. Then the reaction mixture was warmed to 20 o C, and stirred for 2 h. The reaction was then quenched by the addition of aqueous NH 4 Cl solution (10 mL), which was extracted with EtOAc for 3 times. The combined organic phase was dried over anhydrous Na 2 SO 4 , and then concentrated under reduced pressure. The residue was purified by silica gel column chromatography to afford the desired product Example 49g (520 mg, yield: 85%) as yellow oil. LCMS [M+1] + = 406.1.
Step 5: Example 49h
[00591] To a solution of Example 49g (520 mg, 1.35 mmol), NH 2 Boc (224 mg, 1.91 mmol), Cs 2 CO 3 (625 mg, 1.92 mmol) in dioxane (10 mL) were added Pd 2 (dba) 3 (114 mg, 0.12 mmol), and Xantphos (76 mg, 0.13 mmol). The mixture was degassed with N 2 three times, and stirred at 90°C overnight. Then the reaction mixture diluted with EtOAc, washed by water, dried over anhydrous Na 2 SO 4 , and then concentrated under reduced pressure. The residue was purified by silica gel column chromatography to afford the desired product Example 49h (650 mg crude, yield: quant.) as a white solid. LCMS [M+1] + = 465.2.
Step 6: Example 49i
[00592] To a solution of Example 49h (410 mg, 0.88 mmol) in DCM (8 mL) was added TFA (2 mL), which was stirred at r.t. for 2 h. The mixture was concentrated to give the crude product Example 49i (350 mg, crude, yield: quant.) as black oil. LCMS [M+1] + = 265.1.
Step 7: Example 49k
[00593] To a solution of Example 49i (350 mg, 0.76 mmol), Example 49j (248 mg, 0.76 mmol), and TEA (760 mg, 7.6 mmol) in DCM (15 mL) was added HATU (289 mg, 0.76 mmol). The reaction mixture was stirred at r.t. for 2 h. Then DCM (40 mL) was added to the reaction mixture, which was washed with brine (20 mL*2), dried over anhydrous Na 2 SO 4 and concentrated. The residue was purified by silica gel column chromatography to afford the desired product Example 49k (304 mg, yield: 70%) as a brown solid. LCMS [M+1] + = 573.2.
Step 8: Example 49l
[ 00594] To a mixture of Example 49k (304 mg, 0.43 mmol), and Cs 2 CO 3 (260 mg, 0.80 mmol) in dioxane (20 mL) was added 3rd-t-Bu-Xphos-Pd (46.3 mg, 0.053 mmol). The mixture was degassed with N2 three times, and stirred at 80°C for 3 h. Then the reaction mixture was diluted with EtOAc, washed by water, dried over anhydrous Na 2 SO 4 , and then concentrated under reduced pressure to afford crude
Example 49l (200 mg, crude yield: 70%) as a brown solid, which was used in the next step without further purification. LCMS [M+1] + = 537.2.
Step 9: Example 49
[00595] To a solution of Example 49l (200 mg crude, 0.37 mmol) in THF (1.0 mL) was added HCl/MeOH (1.0 mL,6.0 moL/L), which was stirred at r.t. for 3 h. The mixture was concentrated, and the residue was purified by Prep-HPLC to afford the desired product Example 49 (7.8 mg, yield: 5%) as a white solid. LCMS [M+1] + = 437.2. 1 H NMR (400 MHz, DMSO-d6) d 9.95 (s, 1H), 8.32 (d, 1H), 8.26 (s, 1H), 8.16 (s, 1H), 8.00 (d, 1H), 7.77 (s, 1H), 5.81 (s, 1H), 4.58 (q, 2H), 4.00 (t, 2H), 3.90 (s, 1H), 3.56 (d, 1H), 3.43 (t, 1H), 2.90 (d, 3H), 2.56 (t, 2H), 2.09-1.99 (m, 2H), 1.18 (d, 3H).
Example 50:
Step 1: (R)-tert-butyl (1-((4-amino-6-methylpyrimidin-2-yl)methoxy)propan-2-yl) carbamate
[00596] To a solution of 50b ( 90 mg, 0.5 mmol) in THF (4 mL) was added NaH (40 mg, 60%, 2 equiv, 1.0 mmol) at 0 °C. After 20 min, 50a (80 mg, 0.32 mmol) (Studies on the Iodination of 4-Amino-2,6- dimethylpyrimidine-A Possibility of the Regiospecific Functionalization. Journal f. prakt. Chemie. Band 329, Heft 3, 1987, S.400-408 ) was added, then the reaction mixture was warmed to rt. After being stirred at room temperature for 3 h, the reaction mixture was poured into water and then the product was extracted with EA (2×20 mL), dried over Na 2 SO 4 , and concentrated in vacuo. The residue was purified by flash chromatography to afford 1c (56 mg) as a yellow solid. LC-MS (ESI): m/z =297.3 [M+H] + . Step 2: (R)-2-((2-aminopropoxy)methyl)-6-methylpyrimidin-4-amine [00597] A solution of 50c and hydrochloric acid (4M in MeOH ) (3 mL) was stirred at room temperature for 2h. Solvent was evaporated, and the crude product was partitioned between water and DCM. The aqueous layer was basified with NaHCO 3 and extracted with DCM. Combined organic layers were washed with brine, dried over sodium sulfate, filtered, and evaporated to give 50d which was used in the next step without further purification.
Step 3: (R)-tert-butyl (3-((1-((4-amino-6-methylpyrimidin-2-yl)methoxy)propan-2-yl) carbamoyl)-6- chloroimidazo[1,2-b]pyridazin-8-yl)(methyl)carbamate
[00598] To a solution of 50e (66 mg, 0.2 mmol) in DMF (3 mL) were successively added HATU (76 mg, 0.2 mmol) and Et 3 N (36 mg, 0.36 mmol). The reaction mixture was stirred at room temperature for 0.5 h, then the mixture was added 50d (a solution in 1 mL DMF ) and stirred at room temperature for 0.5 h. The mixture was diluted with water (10 mL) and extracted with DCM (10 mL×3). The combined organic layers were then washed with water (10 mL×2) and brine (5 mL×1), dried over Na 2 SO 4 , filtered, and concentrated in vacuo. The residue was purified by flash chromatography to afford the title 50f (81 mg, two steps 85%) as a yellow solid. LC-MS (ESI): m/z =505.3 [M+H] + .
Step 4: tert-butyl ((7R,E)-3 6 ,7-dimethyl-9-oxo-5-oxa-2,8-diaza-1(6,3)-imidazo[1,2-b ]pyridazina- 3(4,2)-pyrimidinacyclononaphane-1 8 -yl)(methyl)carbamate
[00599] To a solution 50f (81 mg, 0.16 mmol) in 1,4-dioxane (4 mL) under argon was successively added Cesium Carbonate (0.13 g, 0.4 mmol), XPhos (24 mg, 0.05 mmol) and Pd2(dba)3 (23 mg, 0.025 mmol). The reaction mixture was heated at 80 °C for 1h. After cooling to room temperature, the mixture was diluted with water and EtOAc. The organic layer was separated and the aqueous layer extracted with EtOAc. The combined organic layers were then washed with water and brine, dried over Na 2 SO 4 , filtered, and concentrated in vacuo. The residue was purified by flash chromatography to afford 50g (40 mg, 53%) as a yellow solid. LC-MS (ESI): m/z =469.3 [M+H] + .
Step 5: (7R,E)-3 6 ,7-dimethyl-1 8 -(methylamino)-5-oxa-2,8-diaza-1(6,3)-imidazo[1,2-b]py ridazina- 3(4,2)-pyrimidinacyclononaphan-9-one
[00600] A solution of 50g (40 mg, 0.085 mmol) and hydrochloric acid (2M in 1,4-dioxane ) (3 mL)) was stirred at room temperature for 2h. Solvent was evaporated, and the crude product was partitioned between water and DCM. The aqueous layer was basified with NaHCO 3 and extracted with DCM.
Combined organic layers were washed with brine, dried over Na 2 SO 4 , filtered, and concentrated in vacuo. The residue was purified by flash chromatography to afford example 50 (12 mg, 38%) as a yellow solid. LC-MS (ESI): m/z =369.2 [M+H] + . 1 H NMR (400 MHz, DMSO-d6) d 10.11 (s, 1H), 9.98 (d, 1H), 7.88 (s, 1H), 7.65 (d, 1H), 6.63 (s, 1H), 5.85 (s, 1H), 4.56 (d, 2H), 3.98-3.84 (m, 1H), 3.68-3.63 (m, 1H), 3.53– 3.43 (m, 1H), 2.91 (d, 3H), 2.35 (s, 3H), 1.23 (s, 3H).
Example 51:
Step 1: 1-(4-methoxy-3-nitrophenyl)-N-methylmethanamine (51b)
[00601] 4-methoxy-3-nitrobenzaldehyde (5 g, 27.6 mmol), CH 3 NH 2 (2.0 g, 64.5 mmol) and AcOH (3 mL) were added to MeOH (50 mL). The reaction mixture was stirred at 0 o C for 1h. NaBH(OAc) 3 (2.0 g, 64.5 mmol) was added and then stirred at rt for overnight. The reaction mixture was then poured into water (200 mL), and was extracted with ethyl acetate. The organic layer was washed with HCl (1M), the aqueous layer was basified with NaHCO 3 and extracted with ethyl acetate, the organic layer was dried over magnesium sulfate, filtered, and concentrated in vacuo to give title compound 51b.(2 g, 44%). LC- MS (ESI): m/z =197.3 [M+H] +
Step 2: tert-butyl(R)-(1-((4-methoxy-3-nitrobenzyl)(methyl)amino)pro pan-2-yl)carbamate (51d)
[00602] 51b (1.6 g,8.16 mmol), tert-butyl(R)-(1-oxopropan-2-yl)carbamate (1.73 g,10.0 mmol) and AcOH (1 mL) was dissolved in methanol (10 mL), the mixture was stirred for 1h. NaBH(OAc)3 (2.0 g, 64.5 mmol) was added and then stirred at rt for overnight. The reaction mixture was then poured into water (100 mL), The aqueous layer was basified with K 2 CO 3 and extracted with ethyl acetate. The organic layer was dried over magnesium sulfate, filtered, and concentrated in vacuo to give title compound 51d (1.05 g, 36.5%). LC-MS (ESI): m/z =354.3 [M+H] +
Step 3: tert-butyl(R)-(1-((3-amino-4-methoxybenzyl)(methyl)amino)pro pan-2-yl)carbamate (51e)
[00603] 51d (1 g, 0.40 mmol), Pd/C (37 mg) was added to MeOH (20 mL) and the mixture was stirred under H 2 ball for overnight, the suspension was diluted with dichloromethane and filtered through Celite. The solvent was removed to give a brown residue which was purified by column chromatography on silica gel to give the title compound 51e (500 mg, 77.4%). LC-MS (ESI): m/z =324.3 [M+H] +
Step 4: (R)-N 1 -(3-amino-4-methoxybenzyl)-N 1 -methylpropane-1,2-diamine (51f)
[00604] To a solution of compound 51e (500 mg,1.55 mmol) in THF (10 mL) was added HCl/dioxane (5 mL), The mixture was stirred at rt for overnight and then was concentrated under reduced pressure, the residue was directly used for the next step without purification. LC-MS (ESI): m/z =224.2 [M+H] +
Step 5: tert-butyl(R)-(3-((1-((3-amino-4-methoxybenzyl)(methyl)amino )propan-2-yl)carbamoyl)-6- chloroimidazo[1,2-b]pyridazin-8-yl)(methyl)carbamate (51g)
[00605] To a solution of compound 2e (345 mg, 1.55 mmol) and 8-((tert-butoxycarbonyl) (methyl) amino)-6-chloroimidazo[1,2-b]pyridazine-3-carboxylic acid (Intermediate B, 600 mg, 1.84 mmol) in DMF (10 mL) was added TEA (3 mL) and HATU (1.52 g, 4 mmol) at room temperature, The reaction mixture was stirred for overnight and then poured into crashed ice, and was extracted with ethyl acetate. The organic layer was dried over magnesium sulfate, filtered, and concentrated in vacuum. The residue was purified column chromatography on silica gel to give the title compound 51g (550 mg, 67%). LC- MS (ESI): m/z =533.3 [M+H] +
Step 6: tert-butyl ((7R,E)-3 6 -methoxy-5,7-dimethyl-9-oxo-2,5,8-triaza-1(6,3)–imid azo [1,2-b] pyridazina-3(1,3)-benzenacyclononaphane-18-yl)(methyl)carbam ate (51h)
[00606] To a solution of 51g (300 mg, 0.56 mmol) in 1,4-dioxane (10 mL) was added Pd 2 (dba) 3 (50 mg, 0.054 mmol), Cs 2 CO 3 (400 mg, 1.22 mmol) and Xphos (30 mg, 0.05 mmol). The reaction mixture was heated to 95 °C and then stirred for 3.5 h under N 2. After cooled to rt, the mixture was filtered. The filtrate was then suspended in 50 mL of water, extracted with ethyl acetate, the organic layer was dried over magnesium sulfate, filtered, and concentrated in vacuum. The residue was purified by column chromatography on silica gel to give the title compound 51h (30 mg, 10.8%). LC-MS (ESI): m/z =496.3 [M+H] +
Step 7: (7R,E)-3 6 -methoxy-5,7-dimethyl-1 8 -(methylamino)-2,5,8-triaza-1(6,3)-imidazo[1,2- b]pyridazina-3(1,3)-benzenacyclononaphan-9-one
[00607] To a solution of 51h (30 mg, 0.06 mmol) in MeOH (2 mL) was added HCl /dioxane (5 mL), The mixture was stirred at rt for overnight and then was concentrated under reduced pressure, the residue was poured into crashed ice, and then K 2 CO 3 was added until PH>10, extracted with ethyl acetate, the organic layer was dried over magnesium sulfate, filtered, and concentrated in vacuum. The residue was purified by column chromatography on silica gel to give example 51 (5 mg, 21.0%). 1 H NMR (400 MHz, DMSO-d6) d 8.73 (d, 1H), 8.36 (s, 1H), 8.18 (d, 1H), 7.78 (s, 1H), 7.37(d, 1H), 6.95 (d, 1H), 6.80 (d, 1H), 6.17 (s, 1H), 4.04-4.00 (m, 1H), 3.86 (s, 3H), 3.74 (d, 1H), 3.12 (d, 1H), 2.87 (d, 3H), 2.46-2.34 (m, 1H), 2.36 (s, 1H), 1.96-1.92 (m, 1H), 1.02 (d, 3H). LC-MS (ESI): m/z =396.3 [M+H] + .
Example 52:
Step1: (R)-tert-butyl (5-chloro-3-((1-((2-fluoro-4-methoxy-5-nitrobenzyl)oxy)propa n-2- yl)carbamoyl)pyrazolo[1,5-a]pyrimidin-7-yl)(methyl)carbamate (52c) [00608] To a solution of Intermediate B (0.24 g, 0.74 mmol) in DMF (10 mL) were successively added HATU (0.31 g, 0.81 mmol) and Et 3 N (0.15 g, 1.5 mmol). The reaction mixture was stirred at room temperature for 0.5 h, then the mixture was added 52a (0.19 g, 0.74 mmol) (a solution in 3 mL DMF ) and stirred at room temperature for 0.5 h. The mixture was diluted with water and extracted with DCM. The combined organic layers were then washed with brine, dried over Na 2 SO 4 , filtered, and concentrated in vacuo. The residue was purified by flash chromatography to afford 52c (0.33 g, 79%) as a yellow solid. LC-MS (ESI): m/z =567.2 [M+H] + .
Step2: (R)-tert-butyl (3-((1-((5-amino-2-fluoro-4-methoxybenzyl)oxy)propan-2-yl)ca rbamoyl)-5- chloropyrazolo[1,5-a]pyrimidin-7-yl)(methyl)carbamate (52d)
[00609] To a solution 52c (0.33 g, 0.58 mmol) in EtOH (20 mL) and water (5 mL) was successively added Iron powder (0.35 g, 6 mmol), NH 4 Cl (18 mg, 0.3 mmol) The reaction mixture was heated at 70 °C for 1h. After cooling to room temperature, Then the reaction mixture was filtered and the solvent was removed. the mixture was diluted with water and EtOAc. The organic layer was separated and the aqueous layer extracted with EtOAc. The combined organic layers were then washed with water and brine, dried over Na 2 SO 4 , filtered, and concentrated in vacuo. The residue was purified by flash chromatography to afford 52d (0.19 g, 61%) as a yellow solid. LC-MS (ESI): m/z =537.3 [M+H] + .
Step3: tert-butyl ((R,1 3 E,1 4 E)-3 4 -fluoro-3 6 -methoxy-7-methyl-9-oxo-5-oxa-2,8-diaza-1(5,3)- pyrazolo[1,5-a]pyrimidina-3(1,3)-benzenacyclononaphane-17-yl )(methyl)carbamate (52e)
[00610] To a solution 52d (0.19 g, 0.35 mmol) in 1,4-dioxane (15 mL) under argon was successively added Cesium Carbonate (0.23 g, 7 mmol), XPhos (78 mg, 0.1 mmol) and Pd2(dba)3 (46 mg, 0.05 mmol). The reaction mixture was heated at 90 °C for 1h. After cooling to room temperature, the mixture was diluted with water and EtOAc. The organic layer was separated and the aqueous layer extracted with EtOAc. The combined organic layers were then washed with water and brine, dried over Na 2 SO 4 , filtered, and concentrated in vacuo. The residue was purified by flash chromatography to afford 3e (0.13 g, 74%) as a yellow solid. LC-MS (ESI): m/z =501.3 [M+H] + .
Step 4: (R,1 3 E,1 4 E)-3 4 -fluoro-3 6 -methoxy-7-methyl-1 7 -(methylamino)-5-oxa-2,8-diaza-1(5,3)- pyrazolo[1,5-a]pyrimidina-3(1,3)-benzenacyclononaphan-9-one
[00611] A solution of 52e (0.13 g, 0.26 mmol) (4 mL) in DCM and hydrochloric acid (2M in 1,4- dioxane) (6 mL) was stirred at room temperature for 2h. Solvent was evaporated, and the crude product was partitioned between water and DCM. The aqueous layer was basified with NaHCO 3 and extracted with DCM. Combined organic layers were washed with brine, dried over sodium sulfate, filtered, and evaporated in vacuo. The residue was purified by flash chromatography to afford example 52 (31 mg, 30%) as a white solid. LC-MS (ESI): m/z =401.3. [M+H] + . 1 H NMR (400 MHz, DMSO) d 8.88 (s, 1H), 8.36 (d, 1H), 8.18 (d, 1H), 8.10 (s, 1H), 7.80 (d, 1H), 6.96 (d, 1H), 5.90 (s, 1H), 4.56 (d, 2H), 3.89 (s, 3H), 3.54 - 3.47 (m, 1H), 3.35 - 3.29 (m, 1H),2.91 (d, 3H), 1.30– 1.19 (m, 1H), 1.15 (d, 3H).
Example 53:
Step 1: methyl 4-methyl-3,5-dinitrobenzoate (53b)
[00612] 4-methyl-3,5-dinitrobenzoic acid (5g, 22.1 mmol) was added to MeOH (50 mL). SOCl 2 (6.6 g, 3.0 mmol) was added dropwise at 0-20 o C. Then the reaction mixture was heated to 60 o C and stirred for 2h. After cooled to rt, the reaction mixture was concentrated in vacuum, the residue was washed with MTBE and dried to give 53b (5g, 94.2%). LC-MS (ESI): m/z =241.3 [M+H] +
Step 2: methyl 3-amino-4-methyl-5-nitrobenzoate (53c)
[00613] 53b (5 g, 20.8 mmol) was added to AcOH (50 mL). Fe (1.68 g, 3.0 mmol) was added by batches and then the mixture was stirred for 1h. The reaction mixture was then poured into water (200 mL) and extracted with ethyl acetate, the organic layer was dried over magnesium sulfate, filtered, and concentrated in vacuum to give the title 53c (4 g, 91.6%). LC-MS (ESI): m/z =211.3 [M+H] +
Step 3: methyl 4-nitro-2H-indazole-6-carboxylate (53d)
[00614] 53c (4 g, 19 mmol) was added to AcOH (50 mL). NaNO 2 (1.72 g in 10 mL H 2 O, 2.5 mmol) was added dropwise and then the mixture was heated to 40 o C for 1h. The reaction mixture was then poured into water (200 mL) and extracted with ethyl acetate, the organic layer was dried over magnesium sulfate, filtered, and concentrated in vacuum to give the title 4d (3 g, 71.5%). LC-MS (ESI): m/z =222.3 [M+H] + Step 4: methyl 2-methyl-4-nitro-2H-indazole-6-carboxylate (53e)
[ 00615] 53d (3 g, 13 mmol) and K 2 CO 3 (2.8 g, 20 mmol) was added to DMF (50 mL). CH 3 I (3.1 g, 22 mmol) was added dropwise and then the mixture was stirred at rt for 1h. The reaction mixture was then poured into water (200 mL) and extracted with ethyl acetate, the organic layer was dried over magnesium sulfate, filtered, and concentrated in vacuum. The residue was purified by column chromatography on silica gel (PE: EA = 2:1) to give 53e (0.7g, 23%) and 4f (0.7g, 23%). LC-MS (ESI): m/z =236.3 [M+H] + Step 5: (2-methyl-4-nitro-2H-indazol-6-yl)methanol (53g)
[00616] 53e (0.7 g, 3.0 mmol) was added to THF (20 mL) under N 2 . After cooled to -60 o C, DIBAL-H (1mol/L in toluene, 6 mL, 6 mmol) was added dropwise and then stirred for 2h. The reaction mixture was then poured into water (100 mL), and was extracted with ethyl acetate. the organic layer was dried over magnesium sulfate, filtered, and concentrated in vacuum. The residue was purified by column chromatography on silica gel (PE: EA = 1:1) to give the 53g (0.4 g, 64.5%. LC-MS (ESI): m/z =208.2 [M+H] +
Step 6: 6-(bromomethyl)-2-methyl-4-nitro-2H-indazole (4h)
[00617] 53g (400 mg, 1.92 mmol) was dissolved in DCM (10 mL), CBr4 (760 mg, 2.3 mmol) and PPh3 (600 mg, 2.3 mmol) were added and then stirred at rt for 2h. The reaction mixture was then poured into water (100 mL) and extracted with ethyl acetate, the organic layer was dried over magnesium sulfate, filtered, and concentrated in vacuum. The residue was purified by column chromatography on silica gel (n-hexane: ethyl acetate = 5:1) to give the title 53h (300 mg, 58.5%). LC-MS (ESI): m/z =271.2 [M+H] + Step 7: tert-butyl (R)-(1-((2-methyl-4-nitro-2H-indazol-6-yl)methoxy) Propan-2-yl) carbamate (53i)
[00618] Tert-butyl (R)-(1-hydroxypropan-2-yl)carbamate (200 mg, 1.15 mmol) was added to THF (20 mL) under N2, NaH (50 mg, 1.3 mmol) was added at 0 o C, the suspension was stirred at rt for 0.5h, 53h (300 mg, 1.10 mmol) was added and then the mixture was stirred at rt for 4h, The reaction mixture was then poured into water (100 mL) and extracted with ethyl acetate, The organic layer was dried over magnesium sulfate, filtered, and concentrated in vacuum. The residue was purified by column chromatography on silica gel (n-hexane: ethyl acetate = 2:1) to give 53i (300 mg, 74.9%). LC-MS (ESI): m/z =365.2 [M+H] +
Step8: tert-butyl(R)-(1-((4-amino-2-methyl-2H-indazol-6-yl)methoxy) propan-2-yl)carbamate (53j)
[00619] 53i (60 mg, 0.16 mmol) and Pd/C (10 mg) was added to MeOH (10 mL) and the mixture was stirred under H 2 ball for overnight, the suspension was diluted with dichloromethane and filtered through Celite. The solvent was removed to give a brown residue which was purified by column chromatography on silica gel (n-hexane: ethyl acetate = 1:1) to give 53j (50 mg, 93.56%). LC-MS (ESI): m/z =335.2 [M+H] +
Step 9: (R)-6-((2-aminopropoxy)methyl)-2-methyl-2H-indazol-4-amine (53k)
[00620] To a solution of 53j (50 mg, 0.15 mmol) in THF(2 mL) was added HCl/dioxane (6 mol/L, 1 mL), The mixture was stirred at rt for overnight and then was concentrated under reduced pressure, the residue was directly used for the next step without purification. LC-MS (ESI): m/z =235.2 [M+H] + Step10: tert-butyl(R)-(3-((1-((4-amino-2-methyl-2H-indazol-6-yl)meth oxy)propan-2-yl) carbamoyl)-5-chloropyrazolo[1,5-a]pyrimidin-7-yl)(methyl)car bamate (53l)
[00621] To a solution of 53k (30 mg, 0.13 mmol) and 7-((tert-butoxycarbonyl)(methyl)amino)-5- chloropyrazolo [1,5-a] pyrimidine- 3-carboxylic acid (42 mg, 0.13 mmol) in DMF (2 mL) was added TEA (0.1 mL) and HATU (50 mg,0.13 mmol) at room temperature, the reaction mixture was stirred for overnight and then poured into crashed ice, and was extracted with ethyl acetate. The organic layer was dried over magnesium sulfate, filtered, and concentrated in vacuum. The residue was purified column chromatography on silica gel (DCM: MeOH = 20:1) to give 53l (20 mg, 28.3%). LC-MS (ESI): m/z =544.3 [M+H] +
Step11: tert-butyl((R,1 3 E,1 4 E,3 4 E)-3 2 ,7-dimethyl-9-oxo-3 2 H-5-oxa-2,8-diaza-1(5,3)-pyrazolo[1,5-a] pyrimidina-3(4,6)-indazolacyclononaphane-17-yl)(methyl)carba mate (53m)
[00622] To a solution of 53l (20 mg, 0.036 mmol) in 1,4-dioxane (2 mL) was added Pd2(dba)3 (10 mg, 0.01 mmol), Cs 2 CO 3 (20 mg, 0.06 mmol) and X-Phos (6 mg, 0.01 mmol). The reaction mixture was heated to 95 °C and then stirred for 3.5 h under N2. After cooled to rt, the mixture was filtered, the filtrate was then suspended in 20 mL of water, extracted with ethyl acetate, the organic layer was dried over magnesium sulfate, filtered, and concentrated in vacuum. The residue was purified by column chromatography on silica gel (DCM: MeOH = 10:1) to give the 53m (15 mg, 82.2%). LC-MS (ESI): m/z =507.3 [M+H] +
Step 12: (R,1 3 E,1 4 E,3 4 E)-3 2 ,7-dimethyl-1 7 -(methylamino)-3 2 H-5-oxa-2,8-diaza-1(5,3)-pyrazolo[1,5- a]pyrimidina-3(4,6)-indazolacyclononaphan-9-one
To a solution of 53m (15 mg, 0.03 mmol) in MeOH (1 mL) was added HCl /dioxane (1 mL), The mixture was stirred at rt for overnight and then was concentrated under reduced pressure, the residue was poured into crashed ice, and then K 2 CO 3 was added until PH>10, extracted with ethyl acetate, the organic layer was dried over magnesium sulfate, filtered, and concentrated in vacuum. The residue was purified by column chromatography on silica gel (DCM: MeOH = 10:1) to give the product (5 mg, 41%). LC-MS (ESI): m/z =407.3 [M+H] +
Example 54:
Step 1: (1-methyl-4-nitro-1H-indazol-6-yl)methanol (54a)
[00623] A solution of methyl 53f (1.5 g, 6.4 mmol) in anhydrous tetrahydrofuran (40 mL) was cooled to -78°C, under a nitrogen atmosphere.1 M Diisobutylaluminum hydride in tetrahydrofuran (13 mL) was then added dropwise and the reaction stirred at -78°C for 30 min, then warmed to room temperature and stirred for 1h After this time, the reaction was carefully treated with 10% NH 4 Cl (20 mL) maintaining the temperature below 25 °C. After the addition was complete, the layers were separated and the aqueous phase was extracted with methylene chloride and the combined organic layers were dried over sodium sulfate. The drying agent was removed by filtration and the filtrate was concentrated under reduced pressure to afford 54a as a yellow solid: which was used in the next step without further purification. Step 2: 6-(bromomethyl)-1-methyl-4-nitro-1H-indazole (54b)
[00624] To a solution of 54a (1.32 g, 6.4mmol) and tetrabromomethane (3.18 g, 9.6mmol) in DCM (20 ml) was added triphenylphosphine (2.52 g, 9.6 mmol) at 0°C and the mixture was stirred at the same temperature for 1 hour. The mixture was evaporated under reduced pressure. The residue was purified by silica gel column chromatography to give 54b (1.2g, 69%). 1 H NMR (400 MHz, CDCl 3 ) d 8.58 (s, 1H), 8.18 (s, 1H), 7.77 (s, 1H), 4.68 (s, 2H), 4.17 (s, 3H).
Step 3: (R)-tert-butyl (1-((1-methyl-4-nitro-1H-indazol-6-yl)methoxy)propan-2-yl)ca rbamate (54c)
[00625] To a solution of 54b (0.53 g, 3 mmol) in THF (20 mL) was added NaH (0.26 g, 60%, 2.2 equiv, 6.6 mmol) at 0 °C. After 20 min, 50b (0.68 g, 2.5 mmol) was added, then the reaction mixture was warmed to rt. After being stirred at room temperature for 2 h, the reaction mixture was poured into water and then the product was extracted with EA (2×50 mL), dried over Na 2 SO 4 , and concentrated in vacuo. The residue was purified by flash chromatography to afford 54c (0.63 g, 69%) as a yellow solid
Step 4: (R)-1-((1-methyl-4-nitro-1H-indazol-6-yl)methoxy)propan-2-am ine (54d)
[00626] A solution of 54c (0.63 g, 1.7 mmol) in DCM (20mL) was added TFA (6 mL)) and stirred at room temperature for 2h. Solvent was evaporated, and the crude product was partitioned between water (30 mL) and DCM (50 mL). The aqueous layer was basified with NaHCO 3 and extracted with DCM (40 mL). Combined organic layers were washed with brine, dried over sodium sulfate, filtered, and evaporated to give 54d which was used in the next step without further purification.
Step 5: (R)-tert-butyl(5-chloro-3-((1-((1-methyl-4-nitro-1H-indazol- 6-yl)methoxy)propan-2- yl)carbamoyl)pyrazolo[1,5-a]pyrimidin-7-yl)(methyl)carbamate (54e)
[00627] To a solution of 54d (0.45 g, 1.7 mmol) and Intermediate B (0.53 g, 1.7 mmol) in DCM (20 mL) were successively added Et 3 N (0.3 g, 3 mmol) and HATU (0.76 g, 2 mmol) The reaction mixture was stirred at room temperature for 0.5 h. Then the mixture was diluted with water (30 mL) and extracted with DCM (40 mL×2). The combined organic layers were then washed with brine (50 mL), dried over Na 2 SO 4 , filtered, and concentrated in vacuo. The residue was purified by flash chromatography to afford the title 54e (0.82 g, 84%) as a yellow solid. LC-MS (ESI): m/z =573.3 [M+H] + .
Step 6: (R)-tert-butyl (3-((1-((4-amino-1-methyl-1H-indazol-6-yl)methoxy)propan-2-y l)carbamoyl)- 5-chloropyrazolo[1,5-a]pyrimidin-7-yl)(methyl)carbamate (54f)
[00628] To a solution of 54e (0.82 g, 1.43 mmol) in EtOH (20 mL) and water (5 mL) was successively added Fe (0.67 g, 12 mmol), NH 4 Cl (54 mg, 1 mmol), The reaction mixture was heated at 80 °C for 40min. After cooling to room temperature, Then the reaction mixture was filtered and the solvent was removed. the mixture was diluted with water (40 mL) and EtOAc (40 mL). The organic layer was separated and the aqueous layer extracted with EtOAc (40 mL). The combined organic layers were then washed with water and brine, dried over Na 2 SO 4 , filtered, and concentrated in vacuo. The residue was purified by flash chromatography to afford 54f (0.53 g, 68%) as a yellow solid. LC-MS (ESI): m/z =543.3 [M+H] + .
Step7: tert-butyl ((R,1 3 E,1 4 E)-3 1 ,7-dimethyl-9-oxo-3 1 H-5-oxa-2,8-diaza-1(5,3)-pyrazolo[1,5- a]pyrimidina-3(4,6)-indazolacyclononaphane-1 7 -yl)(methyl)carbamate (54g)
[00629] To a solution 5f (0.53 g, 0.98 mmol) in 1,4-dioxane (20 mL) under argon was successively added Cesium Carbonate (0.65 g, 2 mmol), XPhos (98 mg, 0.2 mmol) and Pd2(dba)3 (46 mg, 0.5 mmol). The reaction mixture was heated at 80 °C for 1h. After cooling to room temperature, the mixture was diluted with water (30 mL) and EtOAc (30 mL). The organic layer was separated and the aqueous layer extracted with EtOAc (40 mL×2). The combined organic layers were then washed with brine (40 mL), dried over Na 2 SO 4 , filtered, and concentrated in vacuo. The residue was purified by flash
chromatography to afford 54g (0.31 g, 53%) as a yellow solid. LC-MS (ESI): m/z =469.3 [M+H] + . Step 8: (R,1 3 E,1 4 E)-3 1 ,7-dimethyl-1 7 -(methylamino)-3 1 H-5-oxa-2,8-diaza-1(5,3)-pyrazolo[1,5- a]pyrimidina-3(4,6)-indazolacyclononaphan-9-one
[00630] A solution of 54g (0.31 g, 0.61 mmol) in DCM (10 mL) was added TFA (4 mL) was stirred at room temperature for 1h. Solvent was evaporated, and the crude product was partitioned between water (40 mL) and DCM (40 mL). The aqueous layer was basified with NaHCO 3 and extracted with DCM (40 mL×2). Combined organic layers were washed with brine, dried over sodium sulfate, filtered, and evaporated, purified by flash chromatography to afford example 54 (0.13 g, 52%) as a white solid. LC- MS (ESI): m/z =407.3 [M+H] + . 1 H NMR (400 MHz, DMSO-d6) d 9.80 (s, 1H), 8.40 (d, 1H), 8.27 (s, 1H), 8.24 (s, 1H), 8.16 (s, 1H), 7.97 - 7.92 m, 1H), 7.16 (s, 1H), 5.75 (s, 1H), 4.75 (d, 1H), 4.62 (d, 1H)), 4.01 (s, 3H), 3.58 - 3.53 (m, 1H), 3.44 - 3.37 (m, 1H), 2.97 (d, 3H), 1.24 (s, 1H), 1.14 (d, 3H).
Example 55:
Step 1: (5-methoxy-6-nitropyridin-2-yl) methanol (55b)
[00631] Ethyl 5-methoxy-6-nitropicolinate (1 g, 4.42 mmol) was added to MeOH (20 mL). NaBH4 (2.0 g, 64.5 mmol) was added and then stirred at rt for 2h. The reaction mixture was then poured into water (100 mL), and was extracted with ethyl acetate. the organic layer was dried over magnesium sulfate, filtered, and concentrated in vacuo to give 55b (600mg, 74%). LC-MS (ESI): m/z =195.3 [M+H] + Step 2: 6-(bromomethyl)-3-methoxy-2-nitropyridine (55c)
[00632] 55b (600 mg, 3.26 mmol) was dissolved in DCM (10 mL), CBr 4 (1.3 g,3.9 mmol) and PPh 3 (1.0 g,3.9 mmol) were added and then stirred at rt for 2h. The reaction mixture was then poured into water (100 mL) and extracted with ethyl acetate, the organic layer was dried over magnesium sulfate, filtered, and concentrated in vacuum. The residue was purified by column chromatography on silica gel (PE: EA = 5:1) to give 55c (350 mg, 43.7%). LC-MS (ESI): m/z =248.2 [M+H] +
Step 3: tert-butyl (R)-(1-((5-methoxy-6-nitropyridin-2-yl)methoxy)propan-2-yl)c arbamate (55d)
[00633] 50b (200 mg, 1.15 mmol) was added to THF (20 mL) under N 2 ball, NaH (100 mg, 2.5 mmol) was added at 0 o C, the suspension was stirred at rt for 0.5h, 55c (250mg,1.00mmol) was added and then the mixture was stirred at rt for 4h, The reaction mixture was then poured into water (100 mL) and extracted with ethyl acetate, The organic layer was dried over magnesium sulfate, filtered, and concentrated in vacuum. The residue was purified by column chromatography on silica gel (PE: EA = 3:1) to give 55d (300 mg, 64.6%). LC-MS (ESI): m/z =342.2 [M+H] +
Step 4: tert-butyl (R)-(1-((6-amino-5-methoxypyridin-2-yl)methoxy) propan-2-yl) carbamate (55e)
[00634] 55d (250 mg, 0.73 mmol) and Pd/C (30 mg) was added to MeOH (10 mL), the mixture was stirred under H 2 ball for overnight, the suspension was diluted with dichloromethane and filtered through Celite. The solvent was removed to give a brown residue which was purified by column chromatography on silica gel (n-hexane: ethyl acetate = 3:1) to give 55e (200 mg, 88.3%). LC-MS (ESI): m/z =312.3 [M+H] +
Step 5: (R)-5-((2-aminopropoxy) methyl)-2-methoxyaniline (55f)
[00635] To a solution of 55e (200 mg, 1.55 mmol) in THF (10 mL) was added HCl /dioxane (4M, 5 mL), The mixture was stirred at rt for overnight and then was concentrated under reduced pressure, the residue was directly used for the next step without purification. LC-MS (ESI): m/z =212.2 [M+H] + Step 6: tert-butyl (R)-(3-((1-((6-amino-5-methoxypyridin-2-yl)methoxy)propan-2- yl)carbamoyl)-5- chloropyrazolo[1,5-a]pyrimidin-7-yl)(methyl)carbamate (55g)
[00636] To a solution of 55f (100 mg, 0.47 mmol) and Intermediate B (186 mg, 0.57 mmol) in DMF (3 mL) was added TEA (0.5 mL) and HATU (216 mg,0.57 mmol) at room temperature. The reaction mixture was stirred for overnight and then poured into crashed ice, and was extracted with ethyl acetate. The organic layer was dried over magnesium sulfate, filtered, and concentrated in vacuum. The residue was purified column chromatography on silica gel (DCM: MeOH = 20:1) to 55g (100 mg, 41%). LC-MS (ESI): m/z =520.3 [M+H] + .
Step7: tert-butyl ((R,1 3 E,1 4 E)-3 3 -methoxy-7-methyl-9-oxo-5-oxa-2,8-diaza-1(5,3)-pyrazol o[1,5- a]pyrimidina-3(2,6)-pyridinacyclononaphane-17-yl)(methyl)car bamate (55h)
[00637] To a solution of 55g (100 mg, 0.19 mmol) in 1,4-dioxane (30 mL) was added Pd2(dba)3 (30 mg, 0.03 mmol), Cs 2 CO 3 (200 mg, 0.61mmol) and X-Phos (20 mg, 0.03 mmol). The reaction mixture was heated to 95 °C and then stirred for 3.5 h under N2. After cooled to rt, the mixture was filtered, the filtrate was then suspended in 20 mL of water, extracted with ethyl acetate, the organic layer was dried over magnesium sulfate, filtered, and concentrated in vacuum. The residue was purified by column chromatography on silica gel (DCM: MeOH = 10:1) to give the 55h (80 mg, 84.2%). LC-MS (ESI): m/z =484.3 [M+H] +
Step 8: (R,1 3 E,1 4 E)-33-methoxy-7-methyl-1 7 -(methylamino)-5-oxa-2,8-diaza-1(5,3)-pyrazolo[1,5- a]pyrimidina-3(2,6)-pyridinacyclononaphan-9-one
[00638] To a solution of 55h (50 mg, 0.10 mmol) in MeOH (2 mL) was added HCl /dioxane (2M, 5 mL), The mixture was stirred at rt for overnight and then was concentrated under reduced pressure, the residue was poured into crashed ice, and then K 2 CO 3 was added until PH>10, extracted with ethyl acetate, the organic layer was dried over magnesium sulfate, filtered, and concentrated in vacuum. The residue was purified by column chromatography on silica gel (DCM: MeOH = 10:1) to give the Example 55 (20 mg, 52.2%). 1 H NMR (400 MHz, CDCl 3 ) d 8.73 (d, 1H), 8.36 (s, 1H), 8.18 (d, 1H), 7.78 (s, 1H), 7.37(d, 1H), 6.95 (d, 1H), 6.80 (d, 1H), 6.17 (s, 1H), 4.04– 4.00 (m, 1H), 3.86 (s, 3H), 3.74 (d, 1H), 3.12 (d, 1H), 2.87 (d, 3H), 2.46– 2.34 (m, 1H), 2.36 (s, 1H), 1.96– 1.92 (m, 1H),1.02 (d, 3H). LC-MS (ESI): m/z =384.3 [M+H] + .
Example 56:
Step1：(4-fluoro-3-nitro-phenyl)methanol (56-2)
[00639] Sodium borohydride (1.9 g, 35.5 mmol) was added portionwise to a stirring solution of 4- fluoro-3-nitro-benzaldehyde (56-1) (3.0 g, 17.75 mmol) in methanol (100 ml) at 0°C. After 30 min of stirring at r.t., the methanol was removed in vacuo. The residue was treated with cold water and extracted with dichloromethane. The combined organic layer was washed with brine, dried (Na 2 SO 4 ) and then evaporated in vacuo to give the title compound (56-2) as a crude solid (2.5 g, 82%). LC-MS (ESI): m/z =172.1 [M+H]+
Step 2：(3-amino-4-fluoro-phenyl)methanol (56-3)
[00640] (4-fluoro-3-nitro-phenyl)methanol (56-2) (1.0 g, 5.84 mmol) was dissolved in ethanol (9 mL) and H 2 O (3 mL), Fe powder (3.3 g, 58.4 mmol) and NH 4 Cl(4.06 g, 58.4 mmol) were added to solution, then the reaction mixture heated to 85 °C for 3 h, After cooling to room temperature, reaction filtered, filtrate was removed in vacuo. The residue was purified by flash chromatography to afford the title compound (56-3)(0.7 g, 80%) as a white solid. LC-MS (ESI): m/z =142.2 [M+H]+
Step 3：5-[[tert-butyl(dimethyl)silyl]oxymethyl]-2-fluoro-anilin e (56-4)
[00641] To a solution of (3-amino-4-fluoro-phenyl)methanol (56-3) (1.5 g, 10.6 mmol) in DCM was added TBSCl (2.4 g, 15.9 mmol) and imidazole (1.22 g, 18.0 mmol) at 0 o C, the mixture was stirred at r.t. overnight, the mixture treated with cold water and extracted with ethyl acetate. The combined organic layer was washed with brine, dried (Na 2 SO 4 ) and then the residue was purified by flash chromatography to afford the title compound (56-4)(2.24 g, 83%). LC-MS (ESI): m/z =256.2 [M+H]+
Step 4: tert-butyl N-[5-[[tert-butyl(dimethyl)silyl]oxymethyl]-2-fluoro-phenyl] carbamate (56-5)
[00642] To a solution of 56-4 in DCM (2.24 g, 8.78 mmol) was added Boc 2 O (3.8 g, 17.56 mmol), triethylamine (2.66 g, 26.35 mmol) and DMAP (110 mg, 0.9 mmol) at 0 o C, the mixture was stirred at r.t. for 2 h, the mixture treated with cold water and extracted with ethyl acetate. The combined organic layer was washed with brine, dried (Na 2 SO 4 ) and then the residue was purified by flash chromatography to afford the title compound (56-5)(2.1 g, 67%). LC-MS (ESI): m/z =356.1 [M+H]+
Step 5: tert-butyl N-[2-fluoro-5-(hydroxymethyl)phenyl]carbamate (56-6)
[00643] To a solution of 56-5 (2.0 g, 5.63 mmol) in THF was added TBAF (2.9 g, 11.27 mmol) at 0 o C, the mixture was stirred at r.t. for 3 h, the mixture treated with cold water and extracted with ethyl acetate. The combined organic layer was washed with brine, dried (Na 2 SO 4 ) and then the residue was purified by flash chromatography to afford the title compound (56-6)(1.06 g, 78%) as a brown solid.
LC-MS (ESI): m/z =242.2 [M+H]+
Step 6: tert-butyl N-[5-(bromomethyl)-2-fluoro-phenyl]carbamate (56-7)
[00644] Carbon tetrabromide (2.2 g, 6.64 mmol) in anhydrous diethyl ether (5 mL) was added dropwise to a stirred solution of tert-butyl N-[2-fluoro-5-(hydroxymethyl)phenyl]carbamate (56-6) (0.8 g, 3.32 mmol) and Triphenylphosphine (1.74 g, 6.64 mmol) in anhydrous diethyl ether (15 mL). The mixture was stirred overnight before it was concentrated. chromatography with ethyl acetate in hexane (0-10%) gave the title compound (56-7) as pale yellow solid (0.73 g, 72%). LC-MS (ESI): m/z =304.2 [M+H] + Step 7: tert-butyl N-[(1R)-2-[[3-(tert-butoxycarbonylamino)-4-fluoro-phenyl]met hoxy]-1-methyl- ethyl]carbamate (56-8)
[00645] Potassium tert-butoxide (220 mg, 2.0 mmol) was added to a stirred solution of tert-butyl N- [(1R)-2-hydroxy-1-methyl-ethyl] carbamate (350 mg, 2.0 mmol) and tert-butyl N-[5-(bromomethyl)-2- fluoro-phenyl]carbamate (56-7) (400 mg, 1.3 mmol) in THF (15 mL) at 0°C, the mixture was stirred at 75°C for 5 min under microwave. The mixture was treated with cold water and extracted with ethyl acetate. The combined organic layer was washed with brine, dried (Na 2 SO 4 ) and then the residue was purified by flash chromatography to afford the title compound (56-8)(40 mg, 7.6%) as a brown solid. LC- MS (ESI): m/z =399.3 [M+1] +
Step 8: 5-[[(2R)-2-aminopropoxy]methyl]-2-fluoro-aniline (56-9)
[00646] Trifluoroacetic acid (1 mL) was added to a solution of tert-butyl N-[(1R)-2-[[3-(tert- butoxycarbonylamino)-4-fluoro-phenyl]methoxy]-1-methyl-ethyl ]carbamate (56-8) (40 mg, 0.1mmol) in DCM (3 mL), The mixture was stirred 2 h, The mixture solution was evaporated to dryness, then the title compound (56-9) (18 mg.90%) was obtained as brown liquid, which was used in the next step without further purification. LC-MS (ESI): m/z =199.3 [M+H] +
Step 9: tert-butyl N-[3-[[(1R)-2-[(3-amino-4-fluoro-phenyl)methoxy]-1-methyl-et hyl]carbamoyl]-5- chloro-pyrazolo[1,5-a]pyrimidin-7-yl]-N-methyl-carbamate (56-10)
[00647] 5-[[(2R)-2-aminopropoxy]methyl]-2-fluoro-aniline (56-9) (20 mg, 0.1 mmol) was dissolved in DMF (5 mL), TCFH (42 mg, 0.15 mmol), 1-methylimidazole ( 41 mg, 0.5 mmol) and intermediate B（ WO2019023468）(33 mg, 0.1 mmol) were added to the solution in room temperature. After 1 h pf stirring at r.t., the solution mixture was diluted with EA (30 mL), washed with water (2× 30 mL) and brine (30 mL), dried with Na 2 SO 4 and concentrated. The crude product was purified by flash chromatography (PE/EA = 3:1) to afford the title compound (56-10) (30 mg, 59%) as a white solid. LC- MS (ESI): m/z =508.2 [M+H] +
S tep 10: tert-butyl ((R,13E,14E)-36-fluoro-7-methyl-9-oxo-5-oxa-2,8-diaza-1(5,3) -pyrazolo[1,5- a]pyrimidina-3(1,3)-benzenacyclononaphane-1 7 -yl)(methyl)carbamate (56-11)
[00648] To a solution of tert-butyl N-[3-[[(1R)-2-[(3-amino-4-fluoro-phenyl)methoxy]-1-methyl- ethyl]carbamoyl]-5-chloro-pyrazolo[1,5-a]pyrimidin-7-yl]-N-m ethyl-carbamate (56-10) (30 mg, 0.06 mmol) in 1,4-dioxane ( 3 mL) were added Cs 2 CO 3 (40 mg, 0.12 mmol) and 3rd-t-Bu-Xphos-Pd (5 mg). The reaction mixture was stirred at 85°C for 2 h under N 2 . After cooled to room temperature, the solvent was removed, and the residue was purified by silica gel flash column chromatography to afford the product (56-11) (9 mg, 30%) as a white solid. LC-MS (ESI): m/z =471.3 [M+H] +
Step 11: (R,1 3 E,1 4 E)-3 6 -fluoro-7-methyl-1 7 -(methylamino)-5-oxa-2,8-diaza-1(5,3)-pyrazolo[1,5- a]pyrimidina-3(1,3)-benzenacyclononaphan-9-one
[00649] A solution of tert-butyl ((R,1 3 E,1 4 E)-3 6 -fluoro-7-methyl-9-oxo-5-oxa-2,8-diaza-1(5,3)- pyrazolo[1,5-a]pyrimidina-3(1,3)-benzenacyclononaphane-1 7 -yl)(methyl)carbamate (56-11) (9 mg, 0.02 mmol) and trifluoroacetic acid (0.5 mL) in DCM (2 mL) was stirred at room temperature for 3 h. Solvent was evaporated, and the crude product was partitioned between water and DCM. The aqueous layer was basified with NaHCO 3 and extracted with DCM. Combined organic layers were washed with brine, dried over sodium sulfate, filtered, and evaporated, the residue was purified by silica gel flash column chromatography to afford the product example 56 (3 mg, 40%) as a white solid. LC-MS (ESI): m/z =371.2[M+H] + . 1 H NMR (400 MHz, CDCl 3 ) d 8.68 (d, 1H), 8.55 (s, 1H), 8.43-8.39 (m, 1H), 8.11 (s, 1H), 7.08 (dd, 1H), 6.88-6.81 (m, 1H), 6.52-6.46 (m, 1H), 5.42 (s, 1H), 4.58 (dd, 2H), 4.21– 4.16 (m, 1H), 3.63– 3.57 (m, 1H), 3.51– 3.47 (m, 1H), 3.11 (d, 3H), 1.34 (d, 3H).
Example 57
Step 1: methyl 5-bromo-6-oxo-1H-pyridazine-3-carboxylate (57-2)
[00650] To a solution of methyl 6-oxo-1H-pyridazine-3-carboxylate (57-1) (15 g, 97.3 mmol) in AcOH (200 mL) was added AcOK (34 g, 346 mmol) at -10 o C, the mixture was stirred for 20 min, Bromine (34.2 g, 214 mmol) was added dropwise over 20 min, After an additional hour of stirring at 80 o C, The residue was treated with cold water and extracted with dichloromethane. The combined organic layer was washed with brine, dried (Na 2 SO 4 ) and then evaporated in vacuo to give the title compound (57-2) as a crude solid (10.5 g, 46.3%).LC-MS (ESI): m/z =233.1 [M+H] + .
Step 2: methyl 5-bromo-1-methyl-6-oxo-pyridazine-3-carboxylate (57-3)
[00651] To a solution of methyl 5-bromo-6-oxo-1H-pyridazine-3-carboxylate (57-2) (6 g, 26 mmol) in DMF (30 mL) was added Cs 2 CO 3 (17 g, 51 mmol) and Iodomethane (4.4 g, 31 mmol) at 0 o C., the mixture was stirred at r.t. for 4h, The residue was treated with cold water and extracted with EtOAc. The combined organic layer was washed with brine, dried (Na 2 SO 4 ) and then evaporated in vacuo to give the title compound (57-3) as a crude solid (4.5 g, 71%). LC-MS (ESI): m/z =247.0 [M+H] + .
Step 3: methyl 5-(benzylamino)-1-methyl-6-oxo-pyridazine-3-carboxylate (57-4)
[00652] To a solution of methyl 5-bromo-1-methyl-6-oxo-pyridazine-3-carboxylate (57-3) (2.5 g, 10.1 mmol) and phenylmethanamine (1.08 g, 10.1 mmol) in 1,4-dioxane (30 mL) was added Pd 2 (dba) 3 (2.78 g, 3.04 mmol), Cs 2 CO 3 (6.6 g, 20.2 mmol) and Xantphos (3.51 g, 6.07 mmol) under N 2 atmosphere, the mixture was stirred at 100 o C for 4h, The residue was treated with cold water and extracted with EtOAc. The combined organic layer was washed with brine, dried (Na 2 SO 4 ) and then evaporated in vacuo to give the title compound (57-4) (1.6 g, 58%). LC-MS (ESI): m/z =274.0 [M+H] + . Step 4: methyl 5-amino-1-methyl-6-oxo-pyridazine-3-carboxylate (57-5)
[00653] To a solution of methyl 5-(benzylamino)-1-methyl-6-oxo-pyridazine-3-carboxylate (57-4) (2.2 g, 8.0 mmol) in methanol (30 mL) was added10% Pd/C (2.0 g) at r.t. the mixture was exchanged hydrogen three times and stirred at 50 o C for 6h under hydrogen atmosphere, filtered and washed with EA, then evaporated in vacuo to give the title compound (57-5) as a crude solid (1.3 g, 88%). LC-MS (ESI): m/z =184.2 [M+H] + .
Step 5：methyl 5-[bis(tert-butoxycarbonyl)amino]-1-methyl-6-oxo-pyridazine- 3-carboxylate (57-6)
[00654] To a solution of methyl 5-amino-1-methyl-6-oxo-pyridazine-3-carboxylate (57-5) in DCM (1.3 g, 7.1 mmol) was added Boc2O (3.9 g, 18 mmol) and DMAP (0.87 g, 7.1 mmol) at 0 o C, the mixture was stirred at 60 o C for 4 h, the mixture treated with cold water and extracted with ethyl acetate. The combined organic layer was washed with brine, dried (Na 2 SO 4 ) and then the residue was purified by flash chromatography to afford the title compound (57-6)(2.3 g, 85%). LC-MS (ESI): m/z =384.1 [M+H]+ Step 6: tert-butyl N-tert-butoxycarbonyl-N-[6-(hydroxymethyl)-2-methyl-3-oxo-py ridazin-4- yl]carbamate (57-7)
[00655] Sodium borohydride (0.41 g, 11.0 mmol) was added portionwise to a stirring solution of methyl 5-[bis(tert-butoxycarbonyl)amino]-1-methyl-6-oxo-pyridazine- 3-carboxylate (57-6)(2.1 g, 5.48 mmol) in methanol (20 mL) at 0°C. After 2 hours, the methanol was removed in vacuo. The mixture was treated with cold water and extracted with dichloromethane. The combined organic layer was washed with brine, dried (Na 2 SO 4 ) and then evaporated in vacuo, chromatography with ethyl acetate in hexane (0-10%) gave the title compound (57-7) as pale yellow solid (1.6 g, 82%)
LC-MS (ESI): m/z =356.3 [M+H]+
Step 7: tert-butyl N-[6-(bromomethyl)-2-methyl-3-oxo-pyridazin-4-yl]-N-tert-but oxycarbonyl- carbamate (57-8)
[00656] Carbon tetrabromide (3.0 g, 9.0 mmol) in anhydrous DCM (30 mL) was added dropwise to a stirred solution of tert-butyl N-tert-butoxycarbonyl-N-[6-(hydroxymethyl)-2-methyl-3-oxo-py ridazin-4- yl]carbamate (57-7) (1.6 g, 4.5 mmol) and Triphenylphosphine (2.36 g,9.0 mmol) in DCM (15 mL). The mixture was stirred overnight before it was concentrated. chromatography with ethyl acetate in hexane (0-10%) gave the title compound (57-8) as pale yellow solid (1.2 g, 64%)
LC-MS (ESI): m/z =418.2 [M+H] +
Step 8: tert-butyl N-[(1R)-2-[[5-(tert-butoxycarbonylamino)-1-methyl-6-oxo-pyri dazin-3- yl]methoxy]-1-methyl-ethyl]carbamate (57-9)
[00657] Sodium hydride (95 mg, 3.94 mmol) was added portionwise to a stirred solution of tert-butyl N-[(1R)-2-hydroxy-1-methyl-ethyl]carbamate (0.69 g, 3.94 mmol) in THF (15 mL) at 0°C, the mixture was stirred at 0°C for 20 min. then tert-butyl N-tert-butoxycarbonyl-N-[6-(hydroxymethyl)-2-methyl-3- oxo-pyridazin-4-yl]carbamate (57-8) (1.1 g, 2.63 mmol) was added to the mixture at 0°C,after 4h of stirring at r.t., The mixture was treated with cold water and extracted with ethyl acetate. The combined organic layer was washed with brine, dried (Na 2 SO 4 ) and then the residue was purified by flash chromatography to afford the title compound (57-9)(0.9 g, 80%) as a brown solid. LC-MS (ESI): m/z =413.1[M+1] +
Step 9: 4-amino-6-[[(2R)-2-aminopropoxy]methyl]-2-methyl-pyridazin-3 -one (57-10)
[00658] Trifluoroacetic acid (2 mL) was added to a solution of tert-butyl N-[(1R)-2-[[5-(tert- butoxycarbonylamino)-1-methyl-6-oxo-pyridazin-3-yl]methoxy]- 1-methyl-ethyl]carbamate (57-9)(0.6 g, 1.5 mmol) in DCM (5 mL), The mixture was stirred 2 h, The mixture solution was evaporated to dryness, then the title compound (57-10) (0.28 g.91%) was obtained as brown liquid, which was used in the next step without further purification. LC-MS (ESI): m/z =213.2 [M+H] +
Step 10: tert-butyl N-[3-[[(1R)-2-[(5-amino-1-methyl-6-oxo-pyridazin-3-yl)methox y]-1-methyl- ethyl]carbamoyl]-5-chloro-pyrazolo[1,5-a]pyrimidin-7-yl]-N-m ethyl-carbamate (57-11)
[00659] 4-amino-6-[[(2R)-2-aminopropoxy]methyl]-2-methyl-pyridazin-3 -one (57-10) (200 mg, 0.94 mmol) and Intermediate B (308 mg, 0.94 mmol) was dissolved in DMF (5 mL), N,N,N’,N’- Tetramethylchloroformamidinium hexafluorophosphate (397 mg, 1.41 mmol) and 1-methylimidazole ( 387 mg, 4.71 mmol) were added to the solution in room temperature. After 1 h of stirring at r.t., the solution mixture was diluted with EA (30 mL), washed with water (2× 30 mL) and brine (30 mL), dried with Na 2 SO 4 and concentrated. The crude product was purified by flash chromatography (PE/EA = 3:1) to afford the title compound (57-11) (210 mg, 43%) as a white solid.
LC-MS (ESI): m/z =521.1 [M+H] +
Step 11: tert-butyl ((R,1 3 E,1 4 E,3 4 E)-3 1 ,7-dimethyl-3 6 ,9-dioxo-3 1 ,3 6 -dihydro-5-oxa-2,8-diaza-1(5,3)- pyrazolo[1,5-a]pyrimidina-3(5,3)-pyridazinacyclononaphane-1 7 -yl)(methyl)carbamate (57-12)
[00660] To a solution of tert-butyl N-[3-[[(1R)-2-[(5-amino-1-methyl-6-oxo-pyridazin-3-yl)methox y]- 1-methyl-ethyl]carbamoyl]-5-chloro-pyrazolo[1,5-a]pyrimidin- 7-yl]-N-methyl-carbamate (57-11) (200 mg, 0.38 mmol) in 1,4-dioxane (20 mL) were added Cs 2 CO 3 (250 mg, 0.77 mmol) and 3rd-t-Bu-Xphos- Pd (20 mg). The reaction mixture was stirred at 85°C for 2 h under N2. After cooled to room temperature, the solvent was removed, and the residue was purified by silica gel flash column chromatography to afford the product (57-12)(130 mg, 70%) as a white solid.
LC-MS (ESI): m/z =485.0 [M+H] +
Step 12: (R,1 3 E,1 4 E,3 4 E)-3 1 ,7-dimethyl-1 7 -(methylamino)-3 1 ,3 6 -dihydro-5-oxa-2,8-diaza-1(5,3)- pyrazolo[1,5-a]pyrimidina-3(5,3)-pyridazinacyclononaphane-36 ,9-dione
[ 00661] A solution of tert-butyl ((R,1 3 E,1 4 E,3 4 E)-3 1 ,7-dimethyl-3 6 ,9-dioxo-3 1 ,3 6 -dihydro-5-oxa-2,8- diaza-1(5,3)-pyrazolo[1,5-a]pyrimidina-3(5,3)-pyridazinacycl ononaphane-1 7 -yl)(methyl)carbamate (57- 12) (120 mg, 0.25 mmol) and trifluoroacetic acid (1 mL) in DCM (4 mL) was stirred at room temperature for 3 h. Solvent was evaporated, and the crude product was partitioned between water and DCM. The aqueous layer was basified with NaHCO 3 and extracted with DCM. Combined organic layers were washed with brine, dried over sodium sulfate, filtered, and evaporated, the residue was purified by silica gel flash column chromatography to afford the product (50 mg, 53%) as a white solid. LC-MS (ESI): m/z =385.2 [M+H] + . 1 H NMR (400 MHz, DMSO-d 6 ) d 9.75 (s, 1H), 8.48 (s, 1H), 8.23 (s, 1H), 8.17– 8.07 (m, 2H), 6.30 (s, 1H), 4.45 (dd, 2H), 4.05-3.96 (m, 1H), 3.71 (s, 3H), 3.68-3.62 (m, 1H), 3.58-3.53 (m, 1H), 2.92 (d, 3H), 1.18 (d, 3H).
Example 58:
Step 1: tert-butyl N-[3-[[(1R)-2-[(5-amino-1-methyl-6-oxo-pyridazin-3-yl)methox y]-1-methyl- ethyl]carbamoyl]-6-chloro-imidazo[1,2-b]pyridazin-8-yl]-N-me thyl-carbamate (58-1)
[00662] 4-amino-6-[[(2R)-2-aminopropoxy]methyl]-2-methyl-pyridazin-3 -one (57-10)(220 mg, 1.04 mmol) and Intermediate C (340 mg, 1.04 mmol) was dissolved in DMF (5 mL), DIPEA (0.67 g, 5.18 mmol) and HATU ( 0.6 g, 1.55 mmol) were added to the solution in room temperature. After 6 h, the solution mixture was diluted with EA (30 mL), washed with water (2× 30 mL) and brine (30 mL), dried with Na 2 SO 4 and concentrated. The crude product was purified by flash chromatography (PE/EA = 3:1) to afford the title compound (58-1) (0.31 g, 57.4%) as a white solid. LC-MS (ESI): m/z =521.2 [M+H] + Step 2: tert-butyl ((1 5 E,3 4 E,7R)-3 1 ,7-dimethyl-3 6 ,9-dioxo-3 1 ,3 6 -dihydro-5-oxa-2,8-diaza-1(6,3)- imidazo[1,2-b]pyridazina-3(5,3)-pyridazinacyclononaphane-1 8 -yl)(methyl)carbamate (58-2)
[00663] To a solution of tert-butyl N-[3-[[(1R)-2-[(5-amino-1-methyl-6-oxo-pyridazin-3-yl)methox y]- 1-methyl-ethyl]carbamoyl]-6-chloro-imidazo[1,2-b]pyridazin-8 -yl]-N-methyl-carbamate (58-1) (220 mg, 0.42 mmol) in 1,4-dioxane (20 mL) were added Cs 2 CO 3 (275 mg, 0.84 mmol) and 3rd-t-Bu-Xphos-Pd (30 mg). The reaction mixture was stirred at 80 °C for 2 h under N 2 atmosphere. After cooled to room temperature, the solvent was removed, and the residue was purified by silica gel flash column chromatography to afford the product (58-2)(160 mg, 78.2%) as a white solid. LC-MS (ESI): m/z =485.2 [M+H] +
Step 3: (15E,34E,7R)-31,7-dimethyl-18-(methylamino)-31,36-dihydro-5- oxa-2,8-diaza-1(6,3)- imidazo[1,2-b]pyridazina-3(5,3)-pyridazinacyclononaphane-36, 9-dione
[00664] A solution of tert-butyl ((1 5 E,3 4 E,7R)-3 1 ,7-dimethyl-3 6 ,9-dioxo-3 1 ,3 6 -dihydro-5-oxa-2,8-diaza- 1(6,3)-imidazo[1,2-b]pyridazina-3(5,3)-pyridazinacyclononaph ane-1 8 -yl)(methyl)carbamate (58-2) (180 mg, 0.372 mmol) and p-TsOH (192 mg, 1.11 mmol) in DCM (10 mL) was stirred at 40 o C for 1 h. Solvent was evaporated, and the crude product was partitioned between water and DCM. The aqueous layer was basified with NaHCO 3 and extracted with DCM. Combined organic layers were washed with brine, dried over sodium sulfate, filtered, and evaporated, the residue was purified by silica gel flash column chromatography to afford the product example 58 (110 mg, 77%) as a white solid. LC-MS (ESI): m/z =385.2 [M+H] + . 1 H NMR (400 MHz, CDCl 3 ) d 9.50 (s, 1H), 8.58 (d, 1H), 8.14 (s, 1H), 7.89 (s, 1H), 7.72-7.64 (m, 1H), 6.49 (s, 1H), 4.58 (d, 1H), 4.26 (d, 1H), 4.15– 4.03 (m, 1H), 3.71 (s, 3H), 3.64– 3.58 (m, 1H), 3.49– 3.41 (m, 1H), 2.89 (d, 3H), 1.13 (d, 3H).
Example 59:
Step 1: 6-chloro-3-methoxy-pyridazin-4-amine (59-2)
[00665] To a solution of 59-1 (5.0 g, 30.5 mmol) in DMSO (20 mL) were added LiOH (1.46 g, 61 mmol) and methanol (30 mL) at r.t., The reaction mixture was stirred at 80 °C for 12 h. After cooled to room temperature, the mixture was treated with cold water and extracted with ethyl acetate. The combined organic layer was washed with brine, dried (Na 2 SO 4 ) and then the residue was purified by flash chromatography to afford the title compound (59-2)(4.1 g, 85%) as a brown solid. LC-MS (ESI): m/z =160.1 [M+H] +
Step 2: tert-butyl N-(6-chloro-3-methoxy-pyridazin-4-yl)carbamate (59-3)
[00666] To a solution of 6-chloro-3-methoxy-pyridazin-4-amine (59-2) (4.0 g, 25.16 mmol) in DCM was added Boc2O (11.0 g, 50.31 mmol), triethylamine (7.6 g, 75.5 mmol) and DMAP (307 mg, 2.52 mmol) at 0 o C, the mixture was stirred at r.t. for 3 h, the mixture treated with cold water and extracted with DCM. The combined organic layer was washed with brine, dried (Na 2 SO 4 ) and then the residue was purified by flash chromatography to afford the title compound (59-3)(3.71 g, 57%) as a brown solid. LC- MS (ESI): m/z =260.2 [M+H]+
Step 3: tert-butyl N-(3-methoxy-6-vinyl-pyridazin-4-yl)carbamate (59-4)
[00667] To a solution of tert-butyl N-(6-chloro-3-methoxy-pyridazin-4-yl)carbamate (59-3) (3.5 g, 13.51 mmol) and tributyl(vinyl)Tin (8.57 g, 27.03 mmol) in DMF (30 mL) was added CuCl (4.01 g, 40.53 mmol), Pd(PPh3)4 (1.56 g, 1.35 mmol), at r.t. under N2 atmosphere, the mixture was stirred at 80 oC for 4h, then treated with cold water and extracted with EtOAc. The combined organic layer was washed with brine, dried (Na 2 SO 4 ) and then the residue was purified by flash chromatography to afford the title compound (59-4)(2.58 g, 76%). LC-MS (ESI): m/z =252.1 [M+H] +
Step 4: tert-butyl N-(6-formyl-3-methoxy-pyridazin-4-yl)carbamate (59-5)
[00668] To a solution of tert-butyl N-(3-methoxy-6-vinyl-pyridazin-4-yl)carbamate (59-4) (2.5 g, 10 mmol) in DCM (30 mL) was added RuCl 3 (225 mg, 1 mmol) and 4-methylmorpholine N-oxide (3.51 g, 30 mmol) at 0 o C, warmed to r.t. and stirred for 1h, then treated with cold water and extracted with EtOAc. The combined organic layer was washed with brine, dried (Na 2 SO 4 ) and then the residue was purified by flash chromatography to afford the title compound (59-5)(1.87 g, 74%). LC-MS (ESI): m/z =254.1 [M+H] +
Step 5: tert-butyl N-[6-(hydroxymethyl)-3-methoxy-pyridazin-4-yl]carbamate (59-6)
[00669] Sodium borohydride (537 mg, 14.2 mmol) was added portionwise to a stirring solution of tert- butyl N-(6-formyl-3-methoxy-pyridazin-4-yl)carbamate (59-5) (1.8 g,7.1 mmol) in THF (10 mL) at 0°C. After 2 hours of stirring at r.t., the THF was removed in vacuo. The residue was treated with cold water and extracted with dichloromethane. The combined organic layer was washed with brine, dried (Na 2 SO 4 ) and then evaporated in vacuo to give the title compound (59-6) as a crude solid (1.7 g, 94%). LC-MS (ESI): m/z =256.1 [M+H] +
Step 6：tert-butyl N-[6-(bromomethyl)-3-methoxy-pyridazin-4-yl]carbamate (59-7)
[00670] Carbon tetrabromide (4.42 g,13.34 mmol) in anhydrous diethyl ether (5 mL) was added dropwise to a stirred solution of tert-butyl N-[6-(hydroxymethyl)-3-methoxy-pyridazin-4-yl]carbamate (59-6) (1.7 g,6.67 mmol) and Triphenylphosphine (3.50 g,13.34 mmol) in anhydrous diethyl ether (15 mL). The mixture was stirred overnight before it was concentrated. chromatography with ethyl acetate in hexane (0-10%) gave the title compound (59-7) as pale yellow solid (1.08 g, 51%). LC-MS (ESI): m/z =318.1 [M+H] +
Step 7：tert-butyl N-[(1R)-2-[[5-(tert-butoxycarbonylamino)-6-methoxy-pyridazin -3-yl]methoxy]- 1-methyl-ethyl]carbamate (59-8)
[00671] Sodium hydride (273 mg, 6.82 mmol, 60%) was added portionwise to a stirred solution of tert- butyl N-(2-hydroxy-1-methyl-ethyl)carbamate (0.9 g, 5.11 mmol) in THF (15 mL) at 0°C, the mixture was stirred at 0°C for 10 min. then tert-butyl N-[6-(bromomethyl)-3-methoxy-pyridazin-4-yl]carbamate (59-7)(1.08 g, 3.41 mmol) was added to the mixture at 0°C,after 30 min, The mixture was treated with cold water and extracted with ethyl acetate. The combined organic layer was washed with brine, dried (Na 2 SO 4 ) and then the residue was purified by flash chromatography to afford the title compound (59- 8)(930 mg, 66%) as a brown solid. LC-MS (ESI): m/z =413.2 [M+1] +
Step 8: 6-[[(2R)-2-aminopropoxy]methyl]-3-methoxy-pyridazin-4-amine (59-9)
[00672] Trifluoroacetic acid (2 mL) was added to a solution of tert-butyl N-[(1R)-2-[[5-(tert- butoxycarbonylamino)-6-methoxy-pyridazin-3-yl]methoxy]-1-met hyl-ethyl]carbamate (59-8) (200 mg, 0.48 mmol) in DCM (8 mL), The mixture was stirred 2 h, The mixture solution was evaporated to dryness, then the title compound (59-9) (95 mg.92%) was obtained as brown liquid, which was used in the next step without further purification. LC-MS (ESI): m/z =213.2 [M+H] +
Step 9: tert-butyl N-[3-[[(1R)-2-[(5-amino-6-methoxy-pyridazin-3-yl)methoxy]-1- methyl- ethyl]carbamoyl]-5-chloro-pyrazolo[1,5-a]pyrimidin-7-yl]-N-m ethyl-carbamate (59-10)
[00673] 6-[[(2R)-2-aminopropoxy]methyl]-3-methoxy-pyridazin-4-amine (59-9) (95 mg, 0.45 mmol) and Intermediate B (145 mg, 0.45 mmol) was dissolved in DMF (5 mL), HATU (256 mg, 0.67 mmol) and DIPEA ( 116 mg, 0.90 mmol) were added to the solution in room temperature. After 3 h, the solution mixture was diluted with EA (30 mL), washed with water (2× 30 mL) and brine (30 mL), dried with Na 2 SO 4 and concentrated. The crude product was purified by flash chromatography (PE/EA = 3:1) to afford the title compound (59-10) (108 mg, 46%) as a white solid. LC-MS (ESI): m/z =521.0 [M+H]+. Step 10: tert-butyl ((R,1 3 E,1 4 E)-3 6 -methoxy-7-methyl-9-oxo-5-oxa-2,8-diaza-1(5,3)-pyrazol o[1,5- a]pyrimidina-3(5,3)-pyridazinacyclononaphane-1 7 -yl)(methyl)carbamate (59-11)
[00674] To a solution of tert-butyl N-[3-[[(1R)-2-[(5-amino-6-methoxy-pyridazin-3-yl)methoxy]-1- methyl-ethyl]carbamoyl]-5-chloro-pyrazolo[1,5-a]pyrimidin-7- yl]-N-methyl-carbamate (59-10) (108 mg, 0.21 mmol) in 1,4-dioxane (10 mL) were added Cs 2 CO 3 (136 mg, 0.42 mmol) and 3rd-t-Bu-Xphos-Pd (40 mg). The reaction mixture was stirred at 80 °C for 2 h under N2. After cooled to room temperature, the solvent was removed, and the residue was purified by silica gel flash column chromatography to afford the product (59-11)(22 mg, 21.7%) as a white solid. LC-MS (ESI): m/z =485.1 [M+H] +
Step 11: (R,1 3 E,1 4 E)-3 6 -methoxy-7-methyl-1 7 -(methylamino)-5-oxa-2,8-diaza-1(5,3)-pyrazolo[1,5- a]pyrimidina-3(5,3)-pyridazinacyclononaphan-9-one
[00675] A solution of tert-butyl ((R,1 3 E,1 4 E)-3 6 -methoxy-7-methyl-9-oxo-5-oxa-2,8-diaza-1(5,3)- pyrazolo[1,5-a]pyrimidina-3(5,3)-pyridazinacyclononaphane-1 7 -yl)(methyl)carbamate (59-11) (14 mg, 0.044 mmol) and trifluoroacetic acid (0.25 mL) in DCM (2 mL) was stirred at room temperature for 3 h. Solvent was evaporated, and the crude product was partitioned between water and DCM. The aqueous layer was basified with NaHCO 3 and extracted with DCM. Combined organic layers were washed with brine, dried over sodium sulfate, filtered, and evaporated, the residue was purified by silica gel flash column chromatography to afford the product example 59 (5 mg, 50%) as a white solid. LC-MS (ESI): m/z =385.1 [M+H] + . 1 H NMR (400 MHz, CDCl 3 ) d 8.85 (s, 1H), 8.38 (s, 1H), 8.09 (s, 1H), 7.22 (s, 1H), 6.39 (s, 1H), 5.51 (s, 1H), 4.80 (dd, 2H), 4.24 (s, 3H), 4.22-4.18 (m, 1H), 3.73-3.68 (m, 1H), 3.62– 3.55 (m, 1H), 3.13 (d, 3H), 1.35 (d, 3H).
Example 60:
Step1：methyl 8-(tert-butoxycarbonylamino)imidazo[1,2-a]pyridine-6-carboxy late (60-2)
[00676] To a solution of (60-1) (2.0 g, 7.84 mmol) and tert-butyl carbamate (1.41 g, 11.76 mmol) in 1,4-dioxane (100 mL) were added t-BuONa (1.2 g, 11.76 mmol), Pd2(dba)3 (1.44 g, 1.57 mmol) and Dpephos (1.7 g, 3.14 mmol) under N2 atmosphere. The reaction mixture was stirred at 80 °C for 2 h under N2. After cooled to room temperature, the solvent was removed, and the residue was purified by silica gel flash column chromatography to afford the product (60-2)(450 mg, 20%) as a white solid. LC- MS (ESI): m/z =292.3 [M+H] +
Step2: tert-butyl N-[6-(hydroxymethyl)imidazo[1,2-a]pyridin-8-yl]carbamate (60-3)
[00677] Sodium borohydride (176 mg, 4.64 mmol) was added portion-wise to a stirring solution of methyl 8-(tert-butoxycarbonylamino)imidazo[1,2-a]pyridine-6-carboxy late (60-2)(0.54 g, 1.86 mmol) in methanol (10 mL) at r.t. then the mixture warmed to 50 o C and stirred for 1 hours, the methanol was removed in vacuo. The residue was treated with cold water and extracted with dichloromethane. The combined organic layer was washed with brine, dried (Na 2 SO 4 ) and then evaporated in vacuo to give the title compound (60-3) as a crude solid (0.27 g, 55%). LC-MS (ESI): m/z =264.3 [M+H] +
Step3: tert-butyl N-[6-(bromomethyl)imidazo[1,2-a]pyridin-8-yl]carbamate (60-4)
[00678] Carbon tetrabromide (0.68 g, 2.06 mmol) in anhydrous diethyl ether (5 mL) was added dropwise to a stirred solution of tert-butyl N-[6-(hydroxymethyl)imidazo[1,2-a]pyridin-8-yl]carbamate (60-3) (0.27 g, 1.03 mmol) and triphephosphine (0.54 g, 2.06 mmol) in anhydrous diethyl ether (15 mL). The mixture was stirred overnight before it was concentrated. chromatography with ethyl acetate in hexane (0-10%) gave the title compound (60-4) as pale yellow solid (0.18 g, 54%). LC-MS (ESI): m/z =327.2 [M+H] +
Step4: tert-butyl N-[(1R)-2-[[8-(tert-butoxycarbonylamino)imidazo[1,2-a]pyridi n-6-yl]methoxy]-1- methyl-ethyl]carbamate (60-5)
[00679] Sodium hydride (44 mg, 1.1 mmol) was added portion-wise to a stirred solution of tert-butyl N-(2-hydroxy-1-methyl-ethyl)carbamate (0.19 g, 1.1 mmol) in THF (15 mL) at 0°C, the mixture was stirred at 0°C for 10 min. then tert-butyl N-[6-(bromomethyl)imidazo[1,2-a]pyridin-8-yl]carbamate (60- 4)(0.18 g, 0.55 mmol) was added to the mixture at 0°C, warmed to r.t. and stirred for 1h, quenched by cold water, and extracted with ethyl acetate. The combined organic layer was washed with brine, dried (Na 2 SO 4 ) and then the residue was purified by flash chromatography to afford the title compound (60- 5)(0.2 g, 86%) as a brown solid. LC-MS (ESI): m/z =421.5 [M+H] +
Step5: 6-[[(2R)-2-aminopropoxy]methyl]imidazo[1,2-a]pyridin-8-amine (60-6)
[00680] Trifluoroacetic acid (1 mL) was added to a solution of tert-butyl N-[(1R)-2-[[8-(tert- butoxycarbonylamino)imidazo[1,2-a]pyridin-6-yl]methoxy]-1-me thyl-ethyl]carbamate (60-5)(0.2 g, 0.48 mmol) in DCM (5 mL), The mixture was stirred at r.t. for 2 h, The mixture solution was evaporated to dryness, then the title compound (60-6) (0.11 g.100%) was obtained as brown liquid, which was used in the next step without further purification. LC-MS (ESI): m/z =221.3 [M+H] +
step6: tert-butyl N-[3-[[(1R)-2-[(8-aminoimidazo[1,2-a]pyridin-6-yl)methoxy]-1 -methyl- ethyl]carbamoyl]-5-chloro-pyrazolo[1,5-a]pyrimidin-7-yl]-N-m ethyl-carbamate (60-7)
[00681] tert-butyl N-[3-[[(1R)-2-[(8-aminoimidazo[1,2-a]pyridin-6-yl)methoxy]-1 -methyl- ethyl]carbamoyl]-5-chloro-pyrazolo[1,5-a]pyrimidin-7-yl]-N-m ethyl-carbamate (60-6)(0.11 g, 0.5 mmol) and Intermediate B (0.32 g, 0.5 mmol) was dissolved in DMF (5 mL), N,N,N’,N’- Tetramethylchloroformamidinium hexafluorophosphate (0.21 g, 0.75 mmol) and 1-methylimidazole ( 0.08 g, 1.0 mmol) were added to the solution in room temperature. After stirring for 3h, the solution mixture was diluted with EA (30 mL), washed with water (2× 10 mL) and brine (10 mL), dried with Na 2 SO 4 and concentrated. The crude product was purified by flash chromatography (PE/EA = 3:1) to afford the title compound (60-7) (0.12 g, 45%) as a white solid. LC-MS (ESI): m/z =529.0 [M+H] +
Step 7: tert-butyl methyl((R,1 3 E,1 4 E,3 7 E)-7-methyl-9-oxo-5-oxa-2,8-diaza-1(5,3)-pyrazolo[1,5- a]pyrimidina-3(8,6)-imidazo[1,2-a]pyridinacyclononaphane-1 7 -yl)carbamate (60-8)
[00682] To a solution of (60-7) (120 mg, 0.23 mmol) in 1,4-dioxane (20 mL) were added Cs 2 CO 3 (150 mg, 0.46 mmol) and 3rd-t-Bu-Xphos-Pd (120 mg). The reaction mixture was stirred at 80°C for 2 h under N2. After cooled to room temperature, the solvent was removed, and the residue was purified by silica gel flash column chromatography to afford the product (60-8)(80 mg, 72%) as a white solid. LC-MS (ESI): m/z =493.5 [M+H] +
Step 8: (R,1 3 E,1 4 E,3 7 E)-7-methyl-1 7 -(methylamino)-5-oxa-2,8-diaza-1(5,3)-pyrazolo[1,5- a]pyrimidina-3(8,6)-imidazo[1,2-a]pyridinacyclononaphan-9-on e
[00683] A solution of tert-butyl methyl((R,1 3 E,1 4 E,3 7 E)-7-methyl-9-oxo-5-oxa-2,8-diaza-1(5,3)- pyrazolo[1,5-a]pyrimidina-3(8,6)-imidazo[1,2-a]pyridinacyclo nonaphane-1 7 -yl)carbamate (60-8) (80mg, 0.16 mmol) and trifluoroacetic acid (0.5 mL) in DCM (4 mL) was stirred at room temperature for 2 h. Solvent was evaporated, and the crude product was partitioned between water and DCM. The aqueous layer was basified with NaHCO 3 and extracted with DCM. Combined organic layers were washed with brine, dried over sodium sulfate, filtered, and evaporated, the residue was purified by silica gel flash column chromatography to afford the product example 60 (20 mg, 29.5%) as a white solid. 1 H NMR (400 MHz, CD 3 OD) d 8.50 (d, 1H), 8.29 (d, 1H), 8.14 (s, 1H), 7.99 (s, 1H), 7.77 (d, 1H), 7.50 (d, 1H), 4.52 (q, 2H), 4.13– 4.03 (m, 1H), 3.59 (dd, 1H), 3.49 (dd, 1H), 2.99 (s, 3H), 1.22 (d, 3H). LC-MS (ESI): m/z =393.5 [M+H] + Example 62:
Step 1: 3-fluoro-4-methoxy-5-nitro-benzaldehyde (62-2)
[00684] 3-fluoro-4-methoxy-benzaldehyde (62-1) (3.6 g, 23.37 mmol ) was dissolved in concentrated sulfuric acid (30 mL) and cooled to -10°C. Concentrated nitric acid (2.5 mL) in concentrated sulfur acid (4 mL) was added dropwise over 20 min. After an additional hour of stirring at below -10°C, the mixture was poured into crushed ice. the precipitate was collected by filtration and partitioned between dichloromethane (40 mL) and saturated sodium hydrogen carbonate (30mL). The organic layer was dried (Na 2 SO 4 ) and evaporated in vacuo to give the title compound (62-2)(1.6 g, 34.23%) as an oil. LC-MS (ESI): m/z =200.1 [M+H] + .
Step 2：(3-fluoro-4-methoxy-5-nitro-phenyl)methanol (62-3)
[00685] To a stirring solution of 3-fluoro-4-methoxy-5-nitro-benzaldehyde (62-2) (1.6 g,8.0 mmol) in methanol (20 mL) was added sodium borohydride (0.38 g,10.04 mmol) portionwise at 0°C. After 2 hours, the methanol was removed in vacuo. The residue was treated with cold water and extracted with dichloromethane. The combined organic layer was washed with brine, dried (Na 2 SO 4 ) and then evaporated in vacuo to give the title compound (62-3) as a crude solid(1.4 g,87.06%). LC-MS (ESI): m/z =202.1 [M+H] +
Step 3：5-(bromomethyl)-1-fluoro-2-methoxy-3-nitro-benzene (62-4).
[00686] To a solution of (3-fluoro-4-methoxy-5-nitro-phenyl)methanol (62-3) (1.4 g, 6.96mmol) and triphephosphine (2.61g, 9.95 mmol) in anhydrous diethyl ether (30 mL) was added carbon tetrabromide (3.3 g, 9.95 mmol) in anhydrous diethyl ether (5 mL) dropwise. The mixture was stirred overnight before it was concentrated down to a sticky oil. Silica gel chromatography gave the title compound (62-4) as a pale yellow solid (1.3g, 70.97%). LC-MS (ESI): m/z =264.1 [M+H] +
Step 4: tert-butylN-[(1R)-2-[(3-fluoro-4-methoxy-5-nitro-phenyl)meth oxy]-1-methyl- ethyl]carbamate (62-5) [00687] To a stirred solution of tert-butyl N-(2-hydroxy-1-methyl-ethyl)carbamate (0.63 g, 3.61 mmol) in THF (15 mL) was added sodium hydride (144 mg, 3.61 mmol) portionwise at 0°C, the mixture was stirred at 0°C for 10 min. then 5-(bromomethyl)-1-fluoro-2-methoxy-3-nitro-benzene (62-4)(0.95 g, 3.61 mmol) was added to the mixture at 0°C, after 30 min, the mixture was quenched with cold water and extracted with ethyl acetate. The combined organic layer was washed with brine, dried (Na 2 SO 4 ) and then the residue was purified by flash chromatography to afford the title compound (62-5)(0.63 g, 48.83%) as a brown solid. LC-MS (ESI): m/z =359.1 [M+H] +
Step 5: (2R)-1-[(3-fluoro-4-methoxy-5-nitro-phenyl)methoxy]propan-2- amine (62-6)
[00688] Trifluoroacetic acid (1.5 mL) was added to a solution of tert-butylN-[(1R)-2-[(3-fluoro-4- methoxy-5-nitro-phenyl)methoxy]-1-methyl-ethyl]carbamate (62-5) (0.63g, 1.76 mmol) in DCM(5 mL), The mixture was stirred 2 h, The mixture solution was evaporated to dryness, then the title compound (62-6) (0.6 g.91.46%) was obtained as brown liquid, which was used in the next step without further purification. LC-MS (ESI): m/z =259.2 [M+H] +
Step 6: tert-butylN-[5-chloro-3-[[(1R)-2-[(3-fluoro-4-methoxy-5-nitr o-phenyl)methoxy]-1-methyl- ethyl]carbamoyl]pyrazolo[1,5-a]pyrimidin-7-yl]-N-methyl-carb amate (62-7)
[00689] (2R)-1-[(3-fluoro-4-methoxy-5-nitro-phenyl)methoxy]propan-2- amine (62-6)(0.6 g, 1.6 mmol) was dissolved in DMF (10 mL),HATU (0.91g, 2.41mmol), DIPEA(0.41g, 3.2 mmol) and intermediate B (0.52 g, 1.6 mmol) were added to the solution in room temperature. After 18h, the solution mixture was diluted with EA (50 mL), washed with water (2×50 mL) and brine (50 mL), dried with Na 2 SO 4 and concentrated. The crude product was purified by flash chromatography to afford the title compound (62- 7) (545 mg, 59.89%) as a white solid. LC-MS (ESI): m/z =567.2 [M+H] +
Step 7: tert-butylN-[3-[[(1R)-2-[(3-amino-5-fluoro-4-methoxy-phenyl) methoxy]-1-methyl- ethyl]carbamoyl]-5-chloro-pyrazolo[1,5-a]pyrimidin-7-yl]-N-m ethyl-carbamate(62-8)
[00690] tert-butylN-[5-chloro-3-[[(1R)-2-[(3-fluoro-4-methoxy-5-nitr o-phenyl)methoxy]-1-methyl- ethyl]carbamoyl]pyrazolo[1,5-a]pyrimidin-7-yl]-N-methyl-carb amate (62-7) (545 mg, 0.96 mmol) was dissolved in ethanol (45 mL) and H 2 O (15 mL), iron powder (540 mg,9.62 mmol) and NH 4 Cl (310 mg,5.77 mmol) were added to solution, then the reaction mixture heated to 85 °C for 3 h, After cooling to room temperature, reaction filtered, filtrate was removed in vacuo, The residue was purified by flash chromatography (PE/EA = 2:1) to afford the title compound (62-8)(450 mg, 87.2%) as a white solid. LC- MS (ESI): m/z =537.1 [M+H] +
Step 8: tert-butyl((7R,E)-3 5 -fluoro-3 6 -methoxy-7-methyl-9-oxo-5-oxa-2,8-diaza-1(6,3)-imidazo [1,2- b]pyridazina-3(1,3)-benzenacyclononaphane-1 8 -yl)(methyl)carbamate (62-9)
[00691] To a solution of (62-8) (450 mg, 0.84 mmol) in 1,4-dioxane (100 mL) were added Cs 2 CO 3 (820 mg, 2.51 mmol) and 3rd-t-Bu-Xphos-Pd (250 mg). The reaction mixture was stirred at 80°C for 2 h under N 2 . After cooled to room temperature, the solvent was removed, and the residue was purified by silica gel flash column chromatography to afford the product (62-9)(220 mg, 52.50%) as a white solid. LC-MS (ESI): m/z =501.3 [M+H] + Step 9: (7R,E)-3 5 -fluoro-3 6 -methoxy-7-methyl-1 8 -(methylamino)-5-oxa-2,8-diaza-1(6, 3)-imidazo[1, 2-b]pyridazina-3(1, 3)-benzenacyclononaphan-9-one
[00692] A solution of (62-9) (220 mg, 0.44 mmol) and trifluoroacetic acid (1 mL) in DCM (5 mL) was stirred at room temperature for 2 h. Solvent was evaporated, and the crude product was partitioned between water and DCM. The aqueous layer was basified with NaHCO 3 and extracted with DCM.
Combined organic layers were washed with brine, dried over sodium sulfate, filtered, and evaporated, the residue was purified by silica gel flash column chromatography to afford the product (71 mg, 40.34%) as a white solid. LC-MS (ESI): m/z =401.3 [M+H] + . 1 H NMR (400 MHz, CDCl 3 ) d 8.56 (s, 1H), 8.40– 8.14 (m, 2H), 7.14 (s, 1H), 6.62 (d, 1H), 6.22 (d, 1H), 5.38 (s, 1H), 4.53 (dd, 2H), 4.18 (dd, 1H), 4.03 (d, 3H), 3.57 (m, 2H), 3.10 (d, 3H), 1.34 (d, 3H). Example A. TYK2 JH2 Domain Binding Assay
[00693] Binding constants for the compounds described herein against the JH2 domain were determined by the following protocol for a KINOMEscan® assay (DiscoveRx). A fusion protein of a partial length construct of human TYK2 (JH2domain-pseudokinase) (amino acids G556 to D888 based on reference sequence NP_003322.3) and the DNA binding domain of NFkB is expressed in transiently transfected HEK293 cells. From these HEK 293 cells, extracts are prepared in M-PER extraction buffer (Pierce) in the presence of Protease Inhibitor Cocktail Complete (Roche) and Phosphatase Inhibitor Cocktail Set II (Merck) per manufacturers' instructions. The TYK2 (JH2domain-pseudokinase) fusion protein is labeled with a chimeric double-stranded DNA tag containing the NFkB binding site fused to an amplicon for qPCR readout, which is added directly to the expression extract (the final concentration of DNA-tag in the binding reaction is 0.1 nM).
[00694] Streptavidin-coated magnetic beads (Dynal M280) are treated with a biotinylated small molecule ligand for 30 minutes at room temperature to generate affinity resins the binding assays. The liganded beads are blocked with excess biotin and washed with blocking buffer (SeaBlock (Pierce), 1% BSA, 0.05% Tween 20, 1 mM DTT) to remove unbound ligand and to reduce nonspecific binding.
[00695] The binding reaction is assembled by combining 16 ml of DNA-tagged kinase extract, 3.8 ml liganded affinity beads, and 0.18 ml test compound (PBS/0.05% Tween 20/10 mM DTT/0.1% BSA/2 mg/ml sonicated salmon sperm DNA)]. Extracts are used directly in binding assays without any enzyme purification steps at a ³10,000-fold overall stock dilution (final DNA-tagged enzyme concentration <0.1 nM). Extracts are loaded with DNA-tag and diluted into the binding reaction in a two-step process. First extracts are diluted 1:100 in 1× binding buffer (PBS/0.05% Tween 20/10 mM DTT/0.1% BSA/2 mg/ml sonicated salmon sperm DNA) containing 10 nM DNA-tag. This dilution is allowed to equilibrate at room temperature for 15 minutes and then subsequently diluted 1:100 in 1× binding buffer. Test compounds were prepared as 111× stocks in 100% DMSO. Kds were determined using an 11-point 3- fold compound dilution series with three DMSO control points. All compounds for Kd measurements are distributed by acoustic transfer (non-contact dispensing) in 100% DMSO. The compounds are then diluted directly into the assays such that the final concentration of DMSO was 0.9%. All reactions are performed in polypropylene 384-well plates. Each was a final volume of 0.02 mL. Assays are incubated with shaking for 1 hour at room temperature. Then the beads are pelleted and washed with wash buffer (1×PBS, 0.05% Tween 20) to remove displaced kinase and test compound. The washed based are re- suspended in elution buffer (1×PBS, 0.05% Tween 20, 0.5 mM non-biotinylated affinity ligand) and incubated at room temperature with shaking for 30 minutes. The kinase concentration in the eluates was measured by qPCR. qPCR reactions are assembled by adding 2.5 mL of kinase eluate to 7.5 mL of qPCR master mix containing 0.15 mM amplicon primers and 0.15 mM amplicon probe. The qPCR protocol consisted of a 10 minute hot start at 95° C., followed by 35 cycles of 95° C. for 15 seconds, 60° C. for 1 minute.
[00696] Test compounds are prepared as 111× stocks in 100% DMSO. Kds were determined using an 11-point 3-fold compound dilution series with three DMSO control points. All compounds for Kd measurements are distributed by acoustic transfer (non-contact dispensing) in 100% DMSO. The compounds are then diluted directly into the assays such that the final concentration of DMSO was 0.9%. The Kds are determined using a compound top concentration of 30,000 nM. Kd measurements are performed in duplicate.
[00697] Binding constants (Kds) were calculated with a standard dose-response curve using the Hill equation:
[00698] The Hill Slope was set to -1. Curves were fitted using a non-linear least square fit with the Levenberg-Marquardt algorithm (Levenberg, K., A method for the solution of certain non-linear problems in least squares, Q. Appl. Math.2, 164-168 (1944)).
[00699] The results are shown in table 1.
TABLE 1
Example B: IL-12 induced pSTAT4 in human PBMC [00700] Fresh Human PBMCs were resuspended in RPMI 1640 medium with 10% FBS. Cells were seeded in a round bottom 96-well plate at the concentration of 200,000 cells/well. A 10-point dilution series of test compound (top dose 10uM, 1:5 dilution) was added to the well using the liquid dispenser (Tecan D300e) and incubated for 1 hour at 37C. Then human IL-12 recombinant protein (R&D Systems) was added to the well at the final concentration of 10 ng/ml and incubated for 15 minutes at 37C. Cell lysates were prepared and analyzed by Phospho STAT4 (Tyr693) Kit (Meso Scale Discovery) following manufacturer's protocol.
[00701] For calculation of the inhibition rate, the relative pSTAT4 signal of each well = pSTAT4 signal of each well - the average pSTAT4 signal of baseline.
[00702] The inhibition% = (the average pSTAT4 signal of IL-12 treatment wells - the relative of pSTAT4 signal in each compound containing well) / the average pSTAT4 signal of IL-12 treatment wells * 100%
[00703] The curve was plotted as the inhibition% (y-axis) vs. compounds concentration (x-axis) and was fitted with log(inhibitor) vs. normalized response -- Variable slope by GraphPad Prism7.0.
[00704] The results are shown in table 2.
TABLE 2
Example C: INFa induced pSTAT3 or pSTAT5 in human PBMC
[00705] Fresh Human PBMCs were resuspended in RPMI 1640 medium with 10% FBS. Cells were seeded in a round bottom 96-well plate at the concentration of 200,000 cells/well. A 10-point dilution series of test compound (top dose 10uM, 1:5 dilution) was added to the well using the liquid dispenser (Tecan D300e) and incubated for 1 hour at 37C. Then human INFa recombinant protein (R&D Systems) was added to the well at the final concentration of 5000 units/ml and incubated for 15 minutes at 37C. Cell lysates were prepared and analyzed by Phospho STAT3 (Tyr705) cellular kit (Cisbio) or Phospho STAT5 (Tyr693) Kit (Meso Scale Discovery) following manufacturer's protocol.
[00706] For calculation of the inhibition rate, the relative pSTAT signal of each well = pSTAT signal of each well - the average pSTAT signal of baseline.
[00707] The inhibition% = (the average pSTAT signal of INFa treatment wells - the relative of pSTAT signal in each compound containing well) / the average pSTAT signal of INFa treatment wells * 100%
[00708] The curve was plotted as the inhibition% (y-axis) vs. compounds concentration (x-axis) and was fitted with log(inhibitor) vs. normalized response -- Variable slope by GraphPad Prism7.0. Control is BMS-986165:
[00709] The results are shown in table 3. TABLE 3
Example D: JAK1 JH2 and JAK2 JH1 Domain Binding Assay
[00710] Similar to the method for TYK2 JH2 binding described above, JAK1 JH2 and JAK2 JH1 domain binding assay was performed using DiscoverX’s KINOMEscan™, but with change of kinase domain. These assays were performed to compare the binding selectivity of test compounds to JAK1 JH2 and JAK2 JH1 domain. The results are shown in table 4.
TABLE 4 Example E: GM-CSF-induced pSTAT5 and IL-2-induced pSTAT5 in human PBMC in human PBMC
[00711] Similar to the method for INFa induced pSTAT5 in human PBMC described above, these assays were performed to check if test compounds have cross-activity to JAK1. JAK2 and JAK3 pathways in human PBMC. The procedure is as described with change of stimuli to 10 ng/ml of GM-CSF or 20 ng/ml of IL-2. The data are shown in Table 5.
TABLE 5
Example F: Pharmacokinetic Studies
[00712] The pharmacokinetics of test compounds were evaluated in male Sprague Dawley rats when administered via oral gavage and IV injection. The test compound was suspended in 0.5% methylcellulose for oral gavage and dissolved in 5% DMSO/5% Solutol/90% saline for IV injection. The animals were fasted overnight before administration. Plasma samples were collected predose and at 0.5, 1, 3, 6, 9, 12, and 24 hours postdose. The samples were analyzed by LC/MS/MS and the concentration of test compound at each timepoint was determined by linear regression. Pharmacokinetic parameters were calculated from the plasma concentrations using Pheonix WinNonlin. The PK results were summarized in the table 6. TABLE 6: Pharmacokinetic parameters of test compounds in Sprague Dawley rats.
Example G: Pharmaceutical Compositions
Example G1: Parenteral Composition
[00713] To prepare a parenteral pharmaceutical composition suitable for administration by injection, 100 mg of a water-soluble salt of a compound described herein is dissolved in DMSO and then mixed with 10 mL of 0.9% sterile saline. The mixture is incorporated into a dosage unit form suitable for administration by injection.
Example G2: Oral Composition
[00714] To prepare a pharmaceutical composition for oral delivery, 100 mg of a compound described herein is mixed with 750 mg of starch. The mixture is incorporated into an oral dosage unit for, such as a hard gelatin capsule, which is suitable for oral administration.
Example G3: Sublingual (Hard Lozenge) Composition [00715] To prepare a pharmaceutical composition for buccal delivery, such as a hard lozenge, mix 100 mg of a compound described herein, with 420 mg of powdered sugar mixed, with 1.6 mL of light corn syrup, 2.4 mL distilled water, and 0.42 mL mint extract. The mixture is gently blended and poured into a mold to form a lozenge suitable for buccal administration.
[00716] The examples and embodiments described herein are for illustrative purposes only and in some embodiments, various modifications or changes are to be included within the purview of disclosure and scope of the appended claims.
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