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Title:
UDP-DEPENDENT GLYCOSYLTRANSFERASE FOR HIGH EFFICIENCY PRODUCTION OF REBAUDIOSIDES
Document Type and Number:
WIPO Patent Application WO/2018/031955
Kind Code:
A3
Abstract:
Provided herein are compositions and methods for improved production of steviol glycosides in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a Setaria italica UDP-glycosyltransferase 40087 or its variant UDP-glycosyltransferase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a UDP-glycosyltransferase sr.UGT_9252778, Bd_UGT10850, and/or Ob_UGT91B1_like. In some embodiments, the host cell further comprises one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing steviol glycosides in the host cell. The compositions and methods described herein provide an efficient route for the heterologous production of steviol glycosides, including but not limited to, rebaudioside D and rebaudioside M.

Inventors:
ZHAO LISHAN (US)
LI WENZONG (US)
WICHMANN GALE (US)
KHANKHOJE ADITI (US)
GARCIA DE GONZALO CHANTAL (US)
MAHATDEJKUL-MEADOWS TINA (US)
JACKSON SHAINA (US)
LEAVELL MICHAEL (US)
PLATT DARREN (US)
Application Number:
PCT/US2017/046637
Publication Date:
March 15, 2018
Filing Date:
August 11, 2017
Export Citation:
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Assignee:
AMYRIS INC (US)
International Classes:
C12N15/81; A23L2/60; C12N9/10; C12N9/90
Domestic Patent References:
WO2016196321A12016-12-08
WO2013022989A22013-02-14
WO2014122227A22014-08-14
WO2014193888A12014-12-04
WO2016038095A22016-03-17
WO2016023844A12016-02-18
Foreign References:
CN104726523A2015-06-24
Other References:
INDRA PRAKASH ET AL: "Development of Next Generation Stevia Sweetener: Rebaudioside M", FOODS, vol. 3, no. 1, 27 February 2014 (2014-02-27), pages 162 - 175, XP055309887, DOI: 10.3390/foods3010162
DATABASE RefSeq [online] 30 November 2015 (2015-11-30), "UDP-glycosyltransferase 91A1 [Setaria italica]", XP002775106, retrieved from NCBI Database accession no. XP_004982059.1
Attorney, Agent or Firm:
PATHAK, Rahul et al. (US)
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