Title:
ULTRA-SENSITIVE NANOPORE STRUCTURE, CHIP, ANALYSIS DEVICE, AMINO ACID DETECTION METHOD, PROTEIN DETECTION METHOD, AND USE
Document Type and Number:
WIPO Patent Application WO/2024/021686
Kind Code:
A1
Abstract:
An ultra-sensitive nanopore structure, a chip, an analysis device, an amino acid detection method, a protein detection method, and a use. The ultra-sensitive nanopore structure relates to a nanopore (8); the pore diameter of the nanopore (8) falls within a range of 0.35-5 nm, and the length of a sensing area of the nanopore (8) falls within a range of 0.3-2 nm, which is approximate to the size of a single amino acid molecule. A substrate (5) of the ultra-sensitive nanopore chip having a pore diameter falling within a range of 5-500 nm is used for supporting the ultra-sensitive nanopore (8). The ultra-sensitive nanopore analysis device implements a single molecule detection limit less than 1 Dalton, implements detection of amino acid molecules at a single molecule level, completes direct detection of 20 natural amino acid molecules and amino acids which are subjected to post-translational chemical modification, provides a strategy of performing technology fusion on the ultra-sensitive nanopore structure and a biological enzyme to implement protein sequencing, and provides a direct solution for improving the spatial resolution of nanopore detection.
Inventors:
FENG JIANDONG (CN)
WANG FUSHI (CN)
WANG FUSHI (CN)
Application Number:
PCT/CN2023/088193
Publication Date:
February 01, 2024
Filing Date:
April 13, 2023
Export Citation:
Assignee:
UNIV ZHEJIANG (CN)
International Classes:
G01N27/26; C12Q1/6869; G01N15/06; G01N33/68
Foreign References:
| US20170058336A1 | 2017-03-02 | |||
| CN108279312A | 2018-07-13 | |||
| US20170059547A1 | 2017-03-02 | |||
| CN105883838A | 2016-08-24 | |||
| CN112014430A | 2020-12-01 |
Attorney, Agent or Firm:
HANGZHOU ZHONGCHENG PATENT AGENCY CO., LTD. (CN)
Download PDF: