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Title:
ULTRASOUND-ASSISTED SIMULATED DIGESTION METHOD OF MILK PROTEIN ACTIVE PEPTIDE AND APPLICATION THEREOF IN HEALTH FOODS
Document Type and Number:
WIPO Patent Application WO/2019/091472
Kind Code:
A1
Abstract:
An ultrasound-assisted simulated digestion method of a milk protein active peptide and an application thereof in health foods, pertaining to the technical field of intensive processing of dairy products and preparation of health foods. The method firstly employs ultrasonic pretreatment of casein and β-lactoglobulin, followed by enzymatic hydrolysis with a protease to prepare casein and β-lactoglobulin polypeptide, and traces the activity of the polypeptide by simulating gastrointestinal digestion, and then simulates absorption by intestinal epithelial cells with Caco-2 cells, to characterize a highly active milk protein polypeptide digested by the gastrointestinal tract and absorbed by the Caco-2 cells simulating absorption by the inner wall of the small intestine. The method has identified five such highly active milk protein polypeptides.

Inventors:
REN, Xiaofeng (No.301, Xuefu RoadZhenjiang, Jiangsu 3, 212013, CN)
LIANG, Qiufang (No.301, Xuefu RoadZhenjiang, Jiangsu 3, 212013, CN)
MA, Haile (No.301, Xuefu RoadZhenjiang, Jiangsu 3, 212013, CN)
DUAN, Yuqing (No.301, Xuefu RoadZhenjiang, Jiangsu 3, 212013, CN)
HE, Ronghai (No.301, Xuefu RoadZhenjiang, Jiangsu 3, 212013, CN)
YANG, Xiaoming (No.301, Xuefu RoadZhenjiang, Jiangsu 3, 212013, CN)
ZHANG, Xi (No.301, Xuefu RoadZhenjiang, Jiangsu 3, 212013, CN)
HOU, Ting (No.301, Xuefu RoadZhenjiang, Jiangsu 3, 212013, CN)
CHEN, Xinxiang (No.301, Xuefu RoadZhenjiang, Jiangsu 3, 212013, CN)
Application Number:
CN2018/114969
Publication Date:
May 16, 2019
Filing Date:
November 12, 2018
Export Citation:
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Assignee:
JIANGSU UNIVERSITY (No.301, Xuefu RoadZhenjiang, Jiangsu 3, 212013, CN)
International Classes:
C12P21/06; A23L33/18; C07K5/083; C07K5/103; C07K5/107
Domestic Patent References:
WO2010125192A12010-11-04
Foreign References:
CN107964034A2018-04-27
CN107964040A2018-04-27
CN103130869A2013-06-05
CN105524966A2016-04-27
Other References:
YANO, S.: "Isolation from a -Zein of Thermolysin Peptides with Angiotensin I-Converting Enzyme Inhibitory Activity", BIOSCI. BIOTECH. BIOCHEM., 31 December 1996 (1996-12-31)
STIMSON, E. R. ET AL.: "Conformational properties of trans Ac-Asn-Pro-Tyr-NHMe and trans Ac-Tyr-Pro-Asn-NHMe in dimethylsulfoxide and in water determined by multinuclear n.m.r. spectroscopy", INT. J. PEPTIDE PROTEIN RES., vol. 27, no. 6, June 1986 (1986-06-01) - 31 December 1986 (1986-12-31), pages 569 - 582, XP055607868, DOI: 10.1111/j.1399-3011.1986.tb01052.x
ZHU QIAN ET AL.: "Progress in Research on Intestinal Absorption Mechanism of Milk Derived ACE Inhibitory Peptides Using Caco-2 Cell Model", FOOD SCIENCE, vol. 34, no. 9, 31 December 2013 (2013-12-31), pages 330 - 335
Attorney, Agent or Firm:
JW IP LAW FIRM (Room 701, Floor 7,Building A5, No.14 Courtyard, Jiuxianqiao Road,,,Chaoyang District, Beijing 5, 100015, CN)
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