Title:
UNDIFFERENTIATED MAINTENANCE CULTURE MATERIAL
Document Type and Number:
WIPO Patent Application WO/2015/111734
Kind Code:
A1
Abstract:
Provided is a method for culturing (a storage method) somatic stem cells which has an extremely low impact on viability and deterioration of differentiation and cell functions, replacing conventionally-implemented freezing preservation methods without using a chemical agent such as DMSO, which affects the differentiation function of somatic stem cells. This method for culturing mesenchymal stem cells involves using: an undifferentiated maintenance culture material for somatic stem cells, said material being characterized by containing polysaccharides derived from natural substances, and a culture medium in which said undifferentiated maintenance culture material is dispersed; a culture medium containing somatic stem cells in which somatic stem cells are floating in the aforementioned culture medium; and said polysaccharides derived from natural substances.
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Inventors:
KIDOAKI SATORU (JP)
TUJI YUKIE (JP)
HAYASHI HISATO (JP)
IWAMA TAKEHISA (JP)
HORIKAWA MASATO (JP)
TUJI YUKIE (JP)
HAYASHI HISATO (JP)
IWAMA TAKEHISA (JP)
HORIKAWA MASATO (JP)
Application Number:
PCT/JP2015/051929
Publication Date:
July 30, 2015
Filing Date:
January 23, 2015
Export Citation:
Assignee:
NISSAN CHEMICAL IND LTD (JP)
UNIV KYUSHU (JP)
UNIV KYUSHU (JP)
International Classes:
C12N5/077
Domestic Patent References:
| WO2013077423A1 | 2013-05-30 | |||
| WO2012131733A2 | 2012-10-04 | |||
| WO2014017513A1 | 2014-01-30 | |||
| WO2008026634A1 | 2008-03-06 | |||
| WO2011040500A1 | 2011-04-07 |
Foreign References:
| JP2008541728A | 2008-11-27 | |||
| JP2009065854A | 2009-04-02 | |||
| JP2013247943A | 2013-12-12 | |||
| JP2013541956A | 2013-11-21 | |||
| JP4385076B2 | 2009-12-16 | |||
| JP2012517823A | 2012-08-09 | |||
| JP2010532167A | 2010-10-07 | |||
| JP2005270891A | 2005-10-06 | |||
| JP5232976B2 | 2013-07-10 |
Other References:
LOU YR. ET AL.: "The use of nanofibrillar cellulose hydrogel as a flexible three- dimensional model to culture human pluripotent stem cells", STEM CELLS DEV., vol. 23, no. 4, 15 February 2014 (2014-02-15), pages 380 - 92, XP055214904
OTSUJI T.G. ET AL.: "A 3D sphere culture system containing functional polymers for large-scale human pluripotent stem cell production", STEM CELL REPORTS, vol. 2, no. 5, 24 April 2014 (2014-04-24), pages 734 - 45, XP055171477
CELL, vol. 126, 2006, pages 677
TISSU ENG PART A, vol. 15, 2009, pages 147
DANIEL EBERLI, REGENERATIVE MEDICINE AND TISSUE ENGINEERING-CELLS AND BIOMATERIALS, 2011
BIOCHEM. BIOPHYS. RES. COMMUN., vol. 322, no. 3, 2004, pages 759 - 765
See also references of EP 3098306A4
OTSUJI T.G. ET AL.: "A 3D sphere culture system containing functional polymers for large-scale human pluripotent stem cell production", STEM CELL REPORTS, vol. 2, no. 5, 24 April 2014 (2014-04-24), pages 734 - 45, XP055171477
CELL, vol. 126, 2006, pages 677
TISSU ENG PART A, vol. 15, 2009, pages 147
DANIEL EBERLI, REGENERATIVE MEDICINE AND TISSUE ENGINEERING-CELLS AND BIOMATERIALS, 2011
BIOCHEM. BIOPHYS. RES. COMMUN., vol. 322, no. 3, 2004, pages 759 - 765
See also references of EP 3098306A4
Attorney, Agent or Firm:
HANABUSA PATENT & TRADEMARK OFFICE (JP)
A patent business corporation is a なぶさ patent trademark office. (JP)
A patent business corporation is a なぶさ patent trademark office. (JP)
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