Title:
UNGLYCOSYLATED LYSOSTAPHIN VARIANT PROTEIN
Document Type and Number:
WIPO Patent Application WO/2015/020765
Kind Code:
A3
Abstract:
Unglycosylated lysostaphin variant protein, nucleic acid molecule, vector and host cell, as well as a method for production of unglycosylated lysostaphin variant protein in a yeast expression system are provided. The proteins are produced in a Pichia
pastoris expression system and have been shown to have activity equivalent to wild-type lysostaphin. The lysostaphin variant proteins can be used as therapeutic proteins for treatment of diseases such as Staphylococcus aureus infection.
Inventors:
GRISWOLD KARL E (US)
ZHAO HONGLIANG (US)
ZHAO HONGLIANG (US)
Application Number:
PCT/US2014/046786
Publication Date:
April 09, 2015
Filing Date:
July 16, 2014
Export Citation:
Assignee:
DARTMOUTH COLLEGE (US)
International Classes:
A61K38/04; C07H21/04; C07K14/00; C12N1/00; C12N9/00
Foreign References:
| US20060216782A1 | 2006-09-28 | |||
| US20120195872A1 | 2012-08-02 | |||
| US20050118159A1 | 2005-06-02 | |||
| US20100303837A1 | 2010-12-02 |
Other References:
HUANG ET AL.: "Site-specific N-glycosylation of caprine lysostaphin restricts its bacteriolytic activity toward Staphylococcus aureus.", ANIM BIOTECHNOL. 2013, vol. 24, no. 2, 27 March 2013 (2013-03-27), pages 129 - 47, XP055237740, DOI: doi:10.1080/10495398.2012.760469
YANG ET AL.: "de novo Design and Synthesis of Candida antarctica Lipase B Gene and a-Factor Leads to High-Level Expression in Pichia pastoris.", PLOS ONE., vol. 8, no. 1, 2013, pages E53939.
GURRAMKONDA ET AL.: "Simple high- cell density fed-batch technique for high-level recombinant protein production with Pichia pastoris: Application to intracellular production of Hepatitis B surface antigen.", MICROB CELL FACT., vol. 8, no. 13., 2009, pages 1 - 8
WU ET AL.: "Lower Temperature Cultures Enlarge the Effects of Vitreoscilla Hemoglobin Expression on Recombinant Pichia pastoris.", INT J MOL SCI., vol. 13, no. 10, 2012, pages 13212 - 26
BAHRAMPOUR ET AL.: "Alteration in protein profile of Pseudomonas aeruginosa (PTSOX4) coated with magnetic Fe304 nanoparticles.", J NANOSTRUCT CHEM., vol. 3, no. 58., July 2013 (2013-07-01), pages 1 - 8
HENDERSON ET AL.: "Occurrence of the human tumor-specific antigen structure Gal.bata1- 3GaINAca- (Thomsen-Friedenreich) and related structures on gut bacteria: Prevalence, immunochemical analysis and structural confirmation.", GLYCOBIOLOGY., vol. 21, no. 10, 2011, pages 1277 - 89, XP055042237, DOI: doi:10.1093/glycob/cwr058
ZHAO ET AL.: "Gene and protein sequence optimization for high-level production of fully active and aglycosylated lysostaphin in Pichia pastoris.", APPL ENVIRON MICROBIOL., vol. 80, no. 9, May 2014 (2014-05-01), pages 2746 - 53, XP055237738, DOI: doi:10.1128/AEM.03914-13
YANG ET AL.: "de novo Design and Synthesis of Candida antarctica Lipase B Gene and a-Factor Leads to High-Level Expression in Pichia pastoris.", PLOS ONE., vol. 8, no. 1, 2013, pages E53939.
GURRAMKONDA ET AL.: "Simple high- cell density fed-batch technique for high-level recombinant protein production with Pichia pastoris: Application to intracellular production of Hepatitis B surface antigen.", MICROB CELL FACT., vol. 8, no. 13., 2009, pages 1 - 8
WU ET AL.: "Lower Temperature Cultures Enlarge the Effects of Vitreoscilla Hemoglobin Expression on Recombinant Pichia pastoris.", INT J MOL SCI., vol. 13, no. 10, 2012, pages 13212 - 26
BAHRAMPOUR ET AL.: "Alteration in protein profile of Pseudomonas aeruginosa (PTSOX4) coated with magnetic Fe304 nanoparticles.", J NANOSTRUCT CHEM., vol. 3, no. 58., July 2013 (2013-07-01), pages 1 - 8
HENDERSON ET AL.: "Occurrence of the human tumor-specific antigen structure Gal.bata1- 3GaINAca- (Thomsen-Friedenreich) and related structures on gut bacteria: Prevalence, immunochemical analysis and structural confirmation.", GLYCOBIOLOGY., vol. 21, no. 10, 2011, pages 1277 - 89, XP055042237, DOI: doi:10.1093/glycob/cwr058
ZHAO ET AL.: "Gene and protein sequence optimization for high-level production of fully active and aglycosylated lysostaphin in Pichia pastoris.", APPL ENVIRON MICROBIOL., vol. 80, no. 9, May 2014 (2014-05-01), pages 2746 - 53, XP055237738, DOI: doi:10.1128/AEM.03914-13
Attorney, Agent or Firm:
LICATA, Jane, Massey et al. (66 E. Main StreetMarlton, NJ, US)
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