Title:
UNIVERSAL CAR-T CELL, PREPARATION METHOD THEREFOR AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2019/076149
Kind Code:
A1
Abstract:
Provided are a universal CAR-T cell, a preparation method therefor and an application thereof. In the universal CAR-T cell, the functions of a T cell antigen receptor (TCR) and major histocompatibility complexes (MHC I and MHC II) in the T cell are inhibited while multi-gene knockout is performed. A gene encoding the TCR comprises TRAC and/or TRBC. Genes encoding the major histocompatibility complexes comprise HLA-A, B2MH and CIITA. The universal CAR-T cell can target relevant markers of specific tumors and inactivate the functions of the TCR and the MHC on the cell surface, can reduce immunological rejection caused by allogeneic cell therapy and safely and effectively remove tumor cells in the diseased human body, and is not affected by the disease or a treatment mode of a patient in use.
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Inventors:
ZHANG WEI (CN)
LI WULING (CN)
ZHANG XIZHEN (CN)
SHAN JUANJUAN (CN)
LI WULING (CN)
ZHANG XIZHEN (CN)
SHAN JUANJUAN (CN)
Application Number:
PCT/CN2018/103714
Publication Date:
April 25, 2019
Filing Date:
September 02, 2018
Export Citation:
Assignee:
CHONGQING PREC BIOTECH COMPANY LIMITED (CN)
International Classes:
C12N5/10; A61K35/17; A61P31/00; A61P35/00; A61P37/02; A61P37/06; A61P37/08; C12N15/867
Domestic Patent References:
| WO2017093969A1 | 2017-06-08 |
Foreign References:
| CN107723275A | 2018-02-23 |
Other References:
XU, JING ET AL.: "T cell -mediated precision cancer immunotherapy", CHINESE JOURNAL OF CANCER BIOTHERAPY, vol. 23, no. 5, 1 October 2016 (2016-10-01), pages 714 - 719, XP055680004, ISSN: 1007-385X, DOI: 10.3872/j.issn.1007-385x.2016.05.022
POIROT, L. ET AL.: "Multiplex Genome-Edited T- cell Manufacturing Platform for ''Off-the-Shelf'' Adoptive T- cell Immunotherapies", CANCER RESEARCH, vol. 75, no. 18, 15 September 2015 (2015-09-15), XP055568648, ISSN: 0008-5472, DOI: 10.1158/0008-5472.CAN-14-3321
"Molecular Cloning Experiment Guide"
See also references of EP 3699268A4
POIROT, L. ET AL.: "Multiplex Genome-Edited T- cell Manufacturing Platform for ''Off-the-Shelf'' Adoptive T- cell Immunotherapies", CANCER RESEARCH, vol. 75, no. 18, 15 September 2015 (2015-09-15), XP055568648, ISSN: 0008-5472, DOI: 10.1158/0008-5472.CAN-14-3321
"Molecular Cloning Experiment Guide"
See also references of EP 3699268A4
Attorney, Agent or Firm:
BEIJING YUANBEN INTELLECTUAL PROPERTY LAW OFFICE (CN)
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