Title:
UROTHELIAL CELL INDUCTION AGENT AND METHOD FOR INDUCING UROTHELIAL CELLS
Document Type and Number:
WIPO Patent Application WO/2021/039972
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide: a method for preparing urothelial cells that are applicable to the treatment of urinary system diseases, in particular diseases caused by damaged urothelial cells, and diseases due to the loss or dysfunction of urothelial cells; urothelial cells prepared by this method; and a medium for inducing (preparing) urothelial cells. In the present invention, urothelial cells prepared by this method for inducing urothelial cells are applicable for the treatment of urinary system diseases, the method including a step for introducing at least one selected from the group consisting of FOXA1 (Forkhead box A1) gene or an expression product thereof, TP63 (tumor protein P63) gene or an expression product thereof, MYCL (L-Myc) gene or an expression product thereof, and KLF4 (Kruppel-like factor 4) gene or an expression product thereof into mammalian somatic cells as an exogenous factor.
Inventors:
INOUE YUUTA (JP)
MAZDA OSAM (JP)
KISHIDA TSUNAO (JP)
UKIMURA OSAMU (JP)
SEKI MAKOTO (JP)
MAZDA OSAM (JP)
KISHIDA TSUNAO (JP)
UKIMURA OSAMU (JP)
SEKI MAKOTO (JP)
Application Number:
PCT/JP2020/032644
Publication Date:
March 04, 2021
Filing Date:
August 28, 2020
Export Citation:
Assignee:
CELLAXIA INC (JP)
International Classes:
A61K48/00; A61K35/22; A61K35/76; A61P13/00; A61P13/02; A61P13/10; A61P35/00; A61P43/00; C12N5/10; C12N15/12; C12N15/85
Domestic Patent References:
| WO2013130769A1 | 2013-09-06 | |||
| WO2017069222A1 | 2017-04-27 |
Foreign References:
| US20180346933A1 | 2018-12-06 | |||
| JP2019159070A | 2019-09-19 |
Other References:
KANG, MIN-YONG ET AL.: "Generation of Bladder Urothelium from Human Pluripotent Stem Cells under Chemically Defined Serum- and Feeder-Free System", INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 15, 2014, pages 7139 - 7157, XP055797059, DOI: 10.3390/ijms15057139
HUSTLER, ARIANNA ET AL.: "Differential transcription factor expression by human epithelial cells of buccal and urothelial derivation", EXPERIMENTAL CELL RESEARCH, vol. 369, 2018, pages 284 - 294, XP085432988, DOI: 10.1016/j.yexcr. 2018.05.03 1
VARLEY, CL ET AL.: "FOXA1 and IRF-1 intermediary transcriptional regulators of PPARy-induced urothelial cytodifferentiation", CELL DEATH AND DIFFERENTIATION, vol. 16, 2009, pages 103 - 114, XP055797062, DOI: 10.1038/cclcl.2008.116
OSBORN, STEPHANIE L. ET AL.: "Induction of Human Embryonic and induced Pluripotent Stem Cells Into Urothelium", STEM CELLS TRANSLATIONAL MEDICINE, vol. 3, 2014, pages 610 - 619, XP055797064, DOI: 10.5966/sctm.2013- 0131
INOUE, YUTA ET AL.: "Direct conversion of fibroblasts into urothelial cells that may be recruited to regenerating mucosa of injured urinary bladder", SCIENTIFIC REPORTS, vol. 9, 25 September 2019 (2019-09-25), XP055797068, DOI: 10.1038/s41598-019- 50388-6
NING J ET AL., CYTOTECHNOLOGY, vol. 63, no. 5, 2011, pages 531 - 539
SHI JG ET AL., CELL TISSUE RES, vol. 347, no. 3, 2012, pages 737 - 746
OSBORN SL ET AL., STEM CELL TRANSL MED, vol. 3, no. 5, 2014, pages 610 - 619
KANG M ET AL., INT J MOL SCI, vol. 15, no. 5, 2014, pages 7139 - 7157
YAMAMOTO K ET AL., PROC NATL ACAD SCI USA, vol. 112, no. 19, 2015, pages 6152 - 6157
KISHIDA TEJIMA AYAMAMOTO KTANAKA SYAMAMOTO TMAZDA 0: "Reprogrammed Functional Brown Adipocytes Ameliorate Insulin Resistance and Dyslipidemia in Diet-Induced Obesity and Type 2 Diabetes", STEM CELL REPORTS, vol. 5, no. 4, 2015, pages 569 - 581, XP055227136, DOI: 10.1016/j.stemcr.2015.08.007
SOWA Y ET AL., STEM CELLS TRANSL MED, vol. 6, no. 4, 2017, pages 1207 - 1216
WAKAO J ET AL., BIOCHEM BIOPHYS RES COMMUN, vol. 488, no. 2, 2017, pages 368 - 373
MASAKI IEDA, JI-DONG FU: " Direct Reprogramming of Fibroblasts into Functional Cardiomyocytes by Defined Factors Paul Delgado-Olguin, Vasanth Vedantham, Yohei Hayashi, Benoit G. Bruneau", DEEPAK SRIVASTAVA CELL, vol. 142, 2010, pages 375 - 386
THOMAS VIERBUCHENAUSTIN OSTERMEIERZHIPING P. PANGYUKO KOKUBUTHOMAS C. SUDHOFMARIUS WERNIG: "Direct conversion of fibroblasts to functional neurons by defined factors", NATURE, vol. 46
PANG ZPYANG NVIERBUCHEN TOSTERMEIER AFUENTES DRYANG TQCITRI ASEBASTIANO VMARRO SSUDHOF TC: "Induction of human neuronal cells by defined transcription factors", NATURE, vol. 476, 2011, pages 220 - 223
KUNIHIKO HIRAMATSUSATORU SASAGAWAHIDETATSU OUTANIKANAKO NAKAGAWAHIDEKI YOSHIKAWANORIYUKI TSUMAKI: "Generation of hyaline cartilaginous tissue from mouse adult dermal fibroblast culture by defined factors", JOURNAL OF CLINICAL INVESTIGATION, vol. 121, 2011, pages 640 - 657
PENGYU HUANGZHIYING HESHUYI JIHUAWANG SUNDAO XIANGCHANGCHENG LIUYIPING HUXINWANGLIJIAN HUI: "Induction of functional hepatocyte-like cells from mouse fibroblasts by defined factors", NATURE, vol. 475, 2011, pages 386 - 389, XP055086662, DOI: 10.1038/nature10116
SAYAKA SEKIYAATSUSHI SUZUKI: "Direct conversion of mouse fibroblasts to hepatocyte-like cells by defined factors", NATURE, vol. 475, 2011, pages 390 - 393, XP055086663, DOI: 10.1038/nature10263
YAMAMOTO KKISHIDA TSATO YNISHIOKA KEJIMA AFUJIWARA HKUBO TYAMAMOTO TKANAMURA NMAZDA 0: "Direct conversion of human fibroblasts into functional osteoblasts by defined factors", PROC NATL ACAD SCI USA., vol. 112, 2015, pages 6152 - 6157, XP055343601, DOI: 10.1073/pnas.1420713112
YAMAMOTO K.SATO Y.HONJO K.ICHIOKA H.OSEKO FSOWA Y.YAMAMOTO T.KANAMURA N.KISHIDA TMAZDA 0: "Generation of directly converted human osteoblasts that are free of exogenous gene and xenogenic protein", J CELL BIOCHEM, vol. 117, 2016, pages 2538 - 2545, XP055459438, DOI: 10.1002/jcb.25546
OSBORN S.L. ET AL., STEM CELLS TRANSL MED, vol. 3, no. 5, 2014, pages 610 - 619
KANG M, INT. J. MOL. SCI., vol. 15, no. 5, 2014, pages 7139 - 7157
IEDA M: "Heart regeneration using reprogramming technology", PROC JPN ACAD SER B PHYS BIOL SCI., vol. 89, no. 3, 2013, pages 118 - 28
IEDA MFU JDDELGADO-OLGUIN PVEDANTHAM VHAYASHI YBRUNEAU BGSRIVASTAVA D: "Direct reprogramming of fibroblasts into functional cardiomyocytes by defined factors", CELL, vol. 142, no. 3, 6 August 2010 (2010-08-06), pages 375 - 86, XP055210905, DOI: 10.1016/j.cell.2010.07.002
QIAN LHUANG YSPENCER CIFOLEY AVEDANTHAM VLIU LCONWAY SJFU JDSRIVASTAVA D: "In vivo reprogramming of murine cardiac fibroblasts into induced cardiomyocytes", NATURE, vol. 485, no. 7400, 31 May 2012 (2012-05-31), pages 593 - 8
NAKAGAWA M ET AL., SCI REP, vol. 4, no. 3594, 2014
LIAO, YSMYTH, G. K.SHI, W: "featureCounts: an efficient general purpose program for assigning sequence reads to genomic features", BIOINFORMATICS, vol. 30, 2014, pages 923 - 930, XP055693027, DOI: 10.1093/bioinformatics/btt656
GE, S. X.SON, E. W.YAO, R: "iDEP: an integrated web application for differential expression and pathway analysis of RNA-Seq data", BMC BIOINFORMATICS, vol. 19, 2018, XP021265584, DOI: 10.1186/s12859-018-2486-6
KANEHISA, M.FURUMICHI, M.TANABE, M.SATO, Y.MORISHIMA, K: "KEGG: new perspectives on genomes, pathways, diseases and drugs", NUCLEIC ACIDS RES, vol. 45, 2017, pages D353 - D361
LUO, W.BROUWER, C: "Pathview: an R/Bioconductor package for pathway-based data integration and visualization", BIOINFORMATICS, vol. 29, 2013, pages 1830 - 1831
HOMAN T ET AL., J PHARMACOL SCI, vol. 121, no. 4, 2013, pages 327 - 337
SHIMIZU T ET AL., ONCOIMMUNOLOGY, vol. 7, no. 5, 2018, pages e1424671
KANDA, Y: "Investigation of the freely available easy-to-use software 'EZR' for medical statistics", BONE. MARROW. TRANSPLANT., vol. 48, 2013, pages 452 - 458
HABUKA, M. ET AL., PLOS. ONE., vol. 10, 2015, pages e0145301
HUSTLER, ARIANNA ET AL.: "Differential transcription factor expression by human epithelial cells of buccal and urothelial derivation", EXPERIMENTAL CELL RESEARCH, vol. 369, 2018, pages 284 - 294, XP085432988, DOI: 10.1016/j.yexcr. 2018.05.03 1
VARLEY, CL ET AL.: "FOXA1 and IRF-1 intermediary transcriptional regulators of PPARy-induced urothelial cytodifferentiation", CELL DEATH AND DIFFERENTIATION, vol. 16, 2009, pages 103 - 114, XP055797062, DOI: 10.1038/cclcl.2008.116
OSBORN, STEPHANIE L. ET AL.: "Induction of Human Embryonic and induced Pluripotent Stem Cells Into Urothelium", STEM CELLS TRANSLATIONAL MEDICINE, vol. 3, 2014, pages 610 - 619, XP055797064, DOI: 10.5966/sctm.2013- 0131
INOUE, YUTA ET AL.: "Direct conversion of fibroblasts into urothelial cells that may be recruited to regenerating mucosa of injured urinary bladder", SCIENTIFIC REPORTS, vol. 9, 25 September 2019 (2019-09-25), XP055797068, DOI: 10.1038/s41598-019- 50388-6
NING J ET AL., CYTOTECHNOLOGY, vol. 63, no. 5, 2011, pages 531 - 539
SHI JG ET AL., CELL TISSUE RES, vol. 347, no. 3, 2012, pages 737 - 746
OSBORN SL ET AL., STEM CELL TRANSL MED, vol. 3, no. 5, 2014, pages 610 - 619
KANG M ET AL., INT J MOL SCI, vol. 15, no. 5, 2014, pages 7139 - 7157
YAMAMOTO K ET AL., PROC NATL ACAD SCI USA, vol. 112, no. 19, 2015, pages 6152 - 6157
KISHIDA TEJIMA AYAMAMOTO KTANAKA SYAMAMOTO TMAZDA 0: "Reprogrammed Functional Brown Adipocytes Ameliorate Insulin Resistance and Dyslipidemia in Diet-Induced Obesity and Type 2 Diabetes", STEM CELL REPORTS, vol. 5, no. 4, 2015, pages 569 - 581, XP055227136, DOI: 10.1016/j.stemcr.2015.08.007
SOWA Y ET AL., STEM CELLS TRANSL MED, vol. 6, no. 4, 2017, pages 1207 - 1216
WAKAO J ET AL., BIOCHEM BIOPHYS RES COMMUN, vol. 488, no. 2, 2017, pages 368 - 373
MASAKI IEDA, JI-DONG FU: " Direct Reprogramming of Fibroblasts into Functional Cardiomyocytes by Defined Factors Paul Delgado-Olguin, Vasanth Vedantham, Yohei Hayashi, Benoit G. Bruneau", DEEPAK SRIVASTAVA CELL, vol. 142, 2010, pages 375 - 386
THOMAS VIERBUCHENAUSTIN OSTERMEIERZHIPING P. PANGYUKO KOKUBUTHOMAS C. SUDHOFMARIUS WERNIG: "Direct conversion of fibroblasts to functional neurons by defined factors", NATURE, vol. 46
PANG ZPYANG NVIERBUCHEN TOSTERMEIER AFUENTES DRYANG TQCITRI ASEBASTIANO VMARRO SSUDHOF TC: "Induction of human neuronal cells by defined transcription factors", NATURE, vol. 476, 2011, pages 220 - 223
KUNIHIKO HIRAMATSUSATORU SASAGAWAHIDETATSU OUTANIKANAKO NAKAGAWAHIDEKI YOSHIKAWANORIYUKI TSUMAKI: "Generation of hyaline cartilaginous tissue from mouse adult dermal fibroblast culture by defined factors", JOURNAL OF CLINICAL INVESTIGATION, vol. 121, 2011, pages 640 - 657
PENGYU HUANGZHIYING HESHUYI JIHUAWANG SUNDAO XIANGCHANGCHENG LIUYIPING HUXINWANGLIJIAN HUI: "Induction of functional hepatocyte-like cells from mouse fibroblasts by defined factors", NATURE, vol. 475, 2011, pages 386 - 389, XP055086662, DOI: 10.1038/nature10116
SAYAKA SEKIYAATSUSHI SUZUKI: "Direct conversion of mouse fibroblasts to hepatocyte-like cells by defined factors", NATURE, vol. 475, 2011, pages 390 - 393, XP055086663, DOI: 10.1038/nature10263
YAMAMOTO KKISHIDA TSATO YNISHIOKA KEJIMA AFUJIWARA HKUBO TYAMAMOTO TKANAMURA NMAZDA 0: "Direct conversion of human fibroblasts into functional osteoblasts by defined factors", PROC NATL ACAD SCI USA., vol. 112, 2015, pages 6152 - 6157, XP055343601, DOI: 10.1073/pnas.1420713112
YAMAMOTO K.SATO Y.HONJO K.ICHIOKA H.OSEKO FSOWA Y.YAMAMOTO T.KANAMURA N.KISHIDA TMAZDA 0: "Generation of directly converted human osteoblasts that are free of exogenous gene and xenogenic protein", J CELL BIOCHEM, vol. 117, 2016, pages 2538 - 2545, XP055459438, DOI: 10.1002/jcb.25546
OSBORN S.L. ET AL., STEM CELLS TRANSL MED, vol. 3, no. 5, 2014, pages 610 - 619
KANG M, INT. J. MOL. SCI., vol. 15, no. 5, 2014, pages 7139 - 7157
IEDA M: "Heart regeneration using reprogramming technology", PROC JPN ACAD SER B PHYS BIOL SCI., vol. 89, no. 3, 2013, pages 118 - 28
IEDA MFU JDDELGADO-OLGUIN PVEDANTHAM VHAYASHI YBRUNEAU BGSRIVASTAVA D: "Direct reprogramming of fibroblasts into functional cardiomyocytes by defined factors", CELL, vol. 142, no. 3, 6 August 2010 (2010-08-06), pages 375 - 86, XP055210905, DOI: 10.1016/j.cell.2010.07.002
QIAN LHUANG YSPENCER CIFOLEY AVEDANTHAM VLIU LCONWAY SJFU JDSRIVASTAVA D: "In vivo reprogramming of murine cardiac fibroblasts into induced cardiomyocytes", NATURE, vol. 485, no. 7400, 31 May 2012 (2012-05-31), pages 593 - 8
NAKAGAWA M ET AL., SCI REP, vol. 4, no. 3594, 2014
LIAO, YSMYTH, G. K.SHI, W: "featureCounts: an efficient general purpose program for assigning sequence reads to genomic features", BIOINFORMATICS, vol. 30, 2014, pages 923 - 930, XP055693027, DOI: 10.1093/bioinformatics/btt656
GE, S. X.SON, E. W.YAO, R: "iDEP: an integrated web application for differential expression and pathway analysis of RNA-Seq data", BMC BIOINFORMATICS, vol. 19, 2018, XP021265584, DOI: 10.1186/s12859-018-2486-6
KANEHISA, M.FURUMICHI, M.TANABE, M.SATO, Y.MORISHIMA, K: "KEGG: new perspectives on genomes, pathways, diseases and drugs", NUCLEIC ACIDS RES, vol. 45, 2017, pages D353 - D361
LUO, W.BROUWER, C: "Pathview: an R/Bioconductor package for pathway-based data integration and visualization", BIOINFORMATICS, vol. 29, 2013, pages 1830 - 1831
HOMAN T ET AL., J PHARMACOL SCI, vol. 121, no. 4, 2013, pages 327 - 337
SHIMIZU T ET AL., ONCOIMMUNOLOGY, vol. 7, no. 5, 2018, pages e1424671
KANDA, Y: "Investigation of the freely available easy-to-use software 'EZR' for medical statistics", BONE. MARROW. TRANSPLANT., vol. 48, 2013, pages 452 - 458
HABUKA, M. ET AL., PLOS. ONE., vol. 10, 2015, pages e0145301
Attorney, Agent or Firm:
TAKASHIMA, Hajime (JP)
Download PDF: