Title:
USE OF ACHROMOPEPTIDASE FOR LYSIS AT ROOM TEMPERATURE
Document Type and Number:
WIPO Patent Application WO/2011/115975
Kind Code:
A3
Abstract:
A process for detecting the presence or absence of gram-positive bacteria in a biological sample. The biological sample can be obtained from any mammal and contains, at a minimum, cellular components. The sample is combined with an enzymatic lysing agent such as achromopeptidase, and lysed at room temperature. Ferric oxide is then added to the sample containing achromopeptidase. A magnetic field is applied to the sample and nucleic acids are extracted from the cellular components. Target nucleic acids, if present, are amplified using techniques such as Polymerase Chain Reaction (PCR) and then used to detect the presence or absence of gram-positive bacteria. Staphylococcus aureus and Streptococcus agalactiae are examples of target bacteria detected by the methods of the present invention.
Inventors:
HILLIGOSS DANIELLE (US)
KELLER LISA M (US)
RAMADAN SAMAH (US)
COADY JAMIE (US)
HELLYER TOBIN J (US)
KELLER LISA M (US)
RAMADAN SAMAH (US)
COADY JAMIE (US)
HELLYER TOBIN J (US)
Application Number:
PCT/US2011/028494
Publication Date:
January 19, 2012
Filing Date:
March 15, 2011
Export Citation:
Assignee:
BECTON DICKINSON CO (US)
HILLIGOSS DANIELLE (US)
KELLER LISA M (US)
RAMADAN SAMAH (US)
COADY JAMIE (US)
HELLYER TOBIN J (US)
HILLIGOSS DANIELLE (US)
KELLER LISA M (US)
RAMADAN SAMAH (US)
COADY JAMIE (US)
HELLYER TOBIN J (US)
International Classes:
C12N15/10; C12N1/06; C12Q1/68; C12S3/20; C12R1/44; C12R1/46
Foreign References:
| US5185242A | 1993-02-09 | |||
| US5508164A | 1996-04-16 | |||
| US6433160B1 | 2002-08-13 | |||
| US20040002594A1 | 2004-01-01 |
Other References:
PAULE ET AL.: "Direct detection of Staphylococcus aureus from adult and neonate nasal swab specimens using real-time polymerase chain reaction.", J MOL DIAGN., vol. 6, no. 3, August 2004 (2004-08-01), pages 191 - 196, XP009045081
SEKAR ET AL.: "An improved protocol for quantification of freshwater Actinobacteria by fluorescence in situ hybridization.", APPL ENVIRON MICROBIOL., vol. 69, no. 5, May 2003 (2003-05-01), pages 2928 - 2935, XP055087056, DOI: doi:10.1128/AEM.69.5.2928-2935.2003
See also references of EP 2547767A4
SEKAR ET AL.: "An improved protocol for quantification of freshwater Actinobacteria by fluorescence in situ hybridization.", APPL ENVIRON MICROBIOL., vol. 69, no. 5, May 2003 (2003-05-01), pages 2928 - 2935, XP055087056, DOI: doi:10.1128/AEM.69.5.2928-2935.2003
See also references of EP 2547767A4
Attorney, Agent or Firm:
BOTOS, Richard, J. et al. (David Littenberg, Krumholz & Mentik, LLP,600 South Avenue Wes, Westfield NJ, US)
Download PDF: