Title:
USE OF CODON-VARIED OLIGONUCLEOTIDE SYNTHESIS FOR SYNTHETIC SEQUENCE RECOMBINATION
Document Type and Number:
WIPO Patent Application WO2001023401
Kind Code:
A3
Abstract:
Methods of providing recombination libraries that include codon-varied oligonucleotide sequences are described. Codon-varied oligonucleotides are synthesized using trinucleotide or mononucleotide phosphoramidite sequences, and are derived from homologous or non-homologous nucleic acid sequences, or combinations of such sequences. Various methods of recombining codon-varied oligonucleotide sequences to expedite artificial evolution are also described. The present invention additionally relates to various integrated systems that are optionally used to automate these recombination methods.
Inventors:
WELCH MARK (US)
NESS JON E (US)
GUSTAFSSON CLAES (US)
STEMMER WILLEM P C (US)
MINSHULL JEREMY (US)
NESS JON E (US)
GUSTAFSSON CLAES (US)
STEMMER WILLEM P C (US)
MINSHULL JEREMY (US)
Application Number:
PCT/US2000/026708
Publication Date:
December 06, 2001
Filing Date:
September 28, 2000
Export Citation:
Assignee:
MAXYGEN INC (US)
WELCH MARK (US)
NESS JON E (US)
GUSTAFSSON CLAES (US)
STEMMER WILLEM P C (US)
MINSHULL JEREMY (US)
WELCH MARK (US)
NESS JON E (US)
GUSTAFSSON CLAES (US)
STEMMER WILLEM P C (US)
MINSHULL JEREMY (US)
International Classes:
C12N15/10; C12N15/11; G06F19/00; (IPC1-7): C12N15/10
Domestic Patent References:
| WO1997020078A1 | 1997-06-05 | |||
| WO1991006643A1 | 1991-05-16 | |||
| WO1996016073A2 | 1996-05-30 |
Other References:
PATTEN P A ET AL: "APPLICATIONS OF DNA SHUFFLING TO PHARMACEUTICALS AND VACCINES", CURRENT OPINION IN BIOTECHNOLOGY,LONDON,GB, vol. 8, 1997, pages 724 - 733, XP002916609, ISSN: 0958-1669
KIM C H ET AL: "Codon optimization for high-level expression of human erythropoietin (EPO) in mammalian cells", GENE: AN INTERNATIONAL JOURNAL ON GENES AND GENOMES,GB,ELSEVIER SCIENCE PUBLISHERS, BARKING, vol. 199, no. 1-2, 15 October 1997 (1997-10-15), pages 293 - 301, XP004126394, ISSN: 0378-1119
KIM C H ET AL: "Codon optimization for high-level expression of human erythropoietin (EPO) in mammalian cells", GENE: AN INTERNATIONAL JOURNAL ON GENES AND GENOMES,GB,ELSEVIER SCIENCE PUBLISHERS, BARKING, vol. 199, no. 1-2, 15 October 1997 (1997-10-15), pages 293 - 301, XP004126394, ISSN: 0378-1119
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