Title:
USE OF LDH GENE-CONTAINING LACTOBACILLUS PLANTARUM IN PREPARING PRODUCT FOR RELIEVING ENTERITIS
Document Type and Number:
WIPO Patent Application WO/2024/056027
Kind Code:
A1
Abstract:
The present invention provides use of an ldh gene-containing lactobacillus plantarum in preparing a product for relieving enteritis. The lactobacillus plantarum or plasmid involved in the present invention is an ldh gene-containing lactobacillus plantarum or plasmid. Experiments prove that the ldh gene has the function of preventing and relieving inflammation. According to the present invention, lactobacillus plantarum AR113 with acetate kinase (ackA), D-lactate dehydrogenase (D-ldh), and L-lactate dehydrogenase (L-ldh) knocked out is taken as a research object. By studying the effect of the knock-out strains on O157:H7-induced colitis, the important role of lactic acid in alleviating tissue damage caused by O157:H7 and protecting the integrity of intestinal epithelial cells is investigated, and lactic acid can effectively relive and prevent different types of enteritis; a lactic acid or a lactate dehydrogenase gene is selected as a screening marker for bacterium screening, and it is concluded that it is the lactic acid that plays a major relieving role in colitis, thereby laying a foundation for a method for screening probiotics with an inflammation relieving function.
Inventors:
AI LIANZHONG (CN)
WANG GUANGQIANG (CN)
XIA YONGJUN (CN)
XIONG ZHIQIANG (CN)
ZHANG HUI (CN)
SONG XIN (CN)
YANG YIJIN (CN)
LIU XINXIN (CN)
WANG GUANGQIANG (CN)
XIA YONGJUN (CN)
XIONG ZHIQIANG (CN)
ZHANG HUI (CN)
SONG XIN (CN)
YANG YIJIN (CN)
LIU XINXIN (CN)
Application Number:
PCT/CN2023/118810
Publication Date:
March 21, 2024
Filing Date:
September 14, 2023
Export Citation:
Assignee:
UNIV OF SHANGHAI FOR SCIENCE AND TECHNOLOGY (CN)
International Classes:
C12N1/21; A23L33/135; A61K35/747; A61P1/00; A61P29/00; C12N15/53; C12N15/54; C12R1/25
Foreign References:
| CN116726056A | 2023-09-12 | |||
| CN112704233A | 2021-04-27 | |||
| KR20130046897A | 2013-05-08 | |||
| CN114231443A | 2022-03-25 | |||
| CN111235070A | 2020-06-05 | |||
| CN114507621A | 2022-05-17 |
Other References:
TIAN MIAOXIN, SHAO JUNLIN, WANG GUANGQIANG, XIONG ZHIQIANG, ZHANG HUI, SONG XIN, AI LIANZHONG, XIA YONGJUN: "Comparative effect of Lactobacillus plantarum AR113 and penicillin on DSS-induced colitis in mice", FOOD AND FERMENTATION INDUSTRIES, vol. 47, no. 17, 15 September 2021 (2021-09-15), pages 105 - 110, XP093146071, ISSN: 0253-990X, DOI: 10.13995/j.cnki.11-1802/ts.026483
XIA YONGJUN; CHEN YAN; WANG GUANGQIANG; YANG YIJIN; SONG XIN; XIONG ZHIQIANG; ZHANG HUI; LAI PHOENCY; WANG SHIJIE; AI LIANZHONG: "Lactobacillus plantarum AR113 alleviates DSS-induced colitis by regulating the TLR4/MyD88/NF-κB pathway and gut microbiota composition", JOURNAL OF FUNCTIONAL FOODS, ELSEVIER BV, NL, vol. 67, 28 February 2020 (2020-02-28), NL , XP086126322, ISSN: 1756-4646, DOI: 10.1016/j.jff.2020.103854
XIA YONGJUN; CHEN YAN; WANG GUANGQIANG; YANG YIJIN; SONG XIN; XIONG ZHIQIANG; ZHANG HUI; LAI PHOENCY; WANG SHIJIE; AI LIANZHONG: "Lactobacillus plantarum AR113 alleviates DSS-induced colitis by regulating the TLR4/MyD88/NF-κB pathway and gut microbiota composition", JOURNAL OF FUNCTIONAL FOODS, ELSEVIER BV, NL, vol. 67, 28 February 2020 (2020-02-28), NL , XP086126322, ISSN: 1756-4646, DOI: 10.1016/j.jff.2020.103854
Attorney, Agent or Firm:
SHANGHAI KESHENG INTELLECTUAL PROPERTY AGENCY LTD. (CN)
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