Title:
USE OF MULTIPLE TARGET NUCLEIC ACID DETECTION METHOD USING CLAMPING PROBE AND DETECTION PROBE
Document Type and Number:
WIPO Patent Application WO/2016/013880
Kind Code:
A1
Abstract:
The present invention relates to an application of a target nucleic acid detection method using a clamping probe and a detection probe. The method of the present invention can effectively detect a small amount of variation or a specific gene sequence contained in a sample by selective amplification and detection of a trace amount of a target gene to be detected while inhibiting amplification of wild-type genes or undesired genes. Also, it is possible to determine a large number of genotypes at the same time through a melting curve analysis. In particular, the method can be used for diagnosis, prognosis and monitoring of the medical condition of a disease, treatment efficacy evaluation, and for aiding nucleic acid and protein delivery studies and so on, through a very small amount of a mutant genotype that is confirmed at a high detection sensitivity. The method of the present invention comprises a step for evaluating the detection of biomarkers such as EGFR, KRAS, NRAS etc. and the presence of mutations of biomarkers using invasive specimens such as tissues as well as non-invasive specimens (blood, urine, sputum, stool, saliva, and cells). The presence of the biomarker and mutations provides a method used for monitoring of the entire cycle of a related disease, disease prognosis and prediction, decision of disease treatment strategy, disease diagnosis/early diagnosis, disease prevention, and development of disease therapeutics.
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Inventors:
CHOI JAE JIN (KR)
YOON JI HYE (KR)
KIM SU NAM (KR)
KIM SUNG KEE (KR)
KIM JIN WOO (KR)
YOON JI HYE (KR)
KIM SU NAM (KR)
KIM SUNG KEE (KR)
KIM JIN WOO (KR)
Application Number:
PCT/KR2015/007656
Publication Date:
January 28, 2016
Filing Date:
July 23, 2015
Export Citation:
Assignee:
PANAGENE INC (KR)
International Classes:
C12Q1/68
Foreign References:
| US20080176226A1 | 2008-07-24 | |||
| KR20150054633A | 2015-05-20 |
Other References:
OH ET AL.: "Detection of low-level KRAS mutations using PNA-mediated asymmetric PCR clamping and melting curve analysis with unlabeled probes", THE JOURNAL OF MOLECULAR DIAGNOSTICS, vol. 12, no. 4, 2010, pages 418 - 424, XP055256008
EL KADER ET AL.: "The KRAS StripAssay for detection of KRAS mutation in Egyptian patients with colorectal cancer (CRC): A pilot study", JOURNAL OF THE EGYPTIAN NATIONAL CANCER INSTITUTE, vol. 25, no. 1, 2013, pages 37 - 41, XP055389243
CHEN ET AL.: "Rapid detection of K-ras mutations in bile by peptide nucleic acid-mediated PCR clamping and melting curve analysis: comparison with restriction fragment length polymorphism analysis", CLINICAL CHEMISTRY, vol. 50, no. 3, 2004, pages 481 - 489, XP055064603, DOI: doi:10.1373/clinchem.2003.024505
MARCHETTI ET AL.: "Clinical implications of KRAS mutations in lung cancer patients treated with tyrosine kinase inhibitors: an important role for mutations in minor clones", NEOPLASIA, vol. 11, no. 10, 2009, pages 1084 - 1092, XP055389246
See also references of EP 3173488A4
EL KADER ET AL.: "The KRAS StripAssay for detection of KRAS mutation in Egyptian patients with colorectal cancer (CRC): A pilot study", JOURNAL OF THE EGYPTIAN NATIONAL CANCER INSTITUTE, vol. 25, no. 1, 2013, pages 37 - 41, XP055389243
CHEN ET AL.: "Rapid detection of K-ras mutations in bile by peptide nucleic acid-mediated PCR clamping and melting curve analysis: comparison with restriction fragment length polymorphism analysis", CLINICAL CHEMISTRY, vol. 50, no. 3, 2004, pages 481 - 489, XP055064603, DOI: doi:10.1373/clinchem.2003.024505
MARCHETTI ET AL.: "Clinical implications of KRAS mutations in lung cancer patients treated with tyrosine kinase inhibitors: an important role for mutations in minor clones", NEOPLASIA, vol. 11, no. 10, 2009, pages 1084 - 1092, XP055389246
See also references of EP 3173488A4
Attorney, Agent or Firm:
KOREANA PATENT FIRM (KR)
특허법인코리아나 (KR)
특허법인코리아나 (KR)
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