Title:
USE OF NEW MIXED MATRIX IN MALDI-MS IDENTIFICATION OF BACTERIA
Document Type and Number:
WIPO Patent Application WO/2023/011246
Kind Code:
A1
Abstract:
Disclosed is the use of a new mixed matrix in the MALDI-MS identification of bacteria. The use comprises the following steps: step one, culturing, for 24 h on a solid culture medium, bacteria to be detected; step two, respectively dissolving CHCA and CHCA-C3 in a 50% acetonitrile solution containing 2.5% trifluoroacetic acid, so as to prepare CHCA and CHCA-C3 matrix liquids; step three, respectively using the CHCA matrix liquid and the CHCA-C3 matrix liquid at a volume ratio of 1 : 2 and uniformly mixing same; step four, uniformly applying the bacteria obtained via culturing in step one onto an MALDI-MS target plate; step five, after the bacteria on the target plate in step four is air-dried, dispensing a mixed matrix solution obtained in step three onto the dried bacteria; and step six, directly performing MALDI analysis after a solvent in step five is volatilized and crystallized. The new MALDI mixed matrix can simultaneously detect hydrophilic proteins and hydrophobic proteins, thereby increasing the number of mass spectrum peaks of an analyte. The new MALDI mixed matrix can more accurately identify bacteria, and realize the rapid and high-sensitivity distinguishing and identification of bacteria, thereby improving the accuracy of distinguishing bacteria by means of MALDI-MS.
Inventors:
GUO XINHUA (CN)
ZHAO NAN (CN)
ZHAO NAN (CN)
Application Number:
PCT/CN2022/107840
Publication Date:
February 09, 2023
Filing Date:
July 26, 2022
Export Citation:
Assignee:
UNIV JILIN (CN)
International Classes:
G01N27/64
Foreign References:
| CN113588771A | 2021-11-02 | |||
| CN106226530A | 2016-12-14 | |||
| CN103483223A | 2014-01-01 | |||
| CN107219110A | 2017-09-29 | |||
| CN112051321A | 2020-12-08 | |||
| JP2005326391A | 2005-11-24 | |||
| CN110487888A | 2019-11-22 | |||
| US20150276756A1 | 2015-10-01 | |||
| JP2010249576A | 2010-11-04 | |||
| JP2019138811A | 2019-08-22 |
Attorney, Agent or Firm:
UNITALEN ATTORNEYS AT LAW (CN)
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