Title:
USE OF POLYMER IN DETECTION AND PREPARATION OF CELLS EXPRESSING CAR
Document Type and Number:
WIPO Patent Application WO/2023/072006
Kind Code:
A1
Abstract:
Provided is the use of a polymer in the detection and preparation of cells expressing CAR. Antigen polymers can achieve the highly specific, sensitive and precise detection of CAR, can be used for sensitive detection of rare CAR-T cells by means of magnetic enrichment, and can also be used for activating phenotypes and specific proliferation by means of temperature control and specific CAR-T cell stimulation and achieving multi-dimensional analysis on CAR-T cells by means of single-cell multiomics analysis. In addition, a method for specifically producing CAR-T cells using antigen polymers is different from the current method for non-specifically activating and proliferating T cells using nonspecific anti-CD28/CD3 magnetic beads. Since the antigen-polymer only specifically activates and proliferates corresponding CAR-T cells and does not activate other T cells, there is no need to worry about the potential toxicity of magnetic beads, which is a better method for preparing CAR-T cells. Further provided is an antigen polymer labeled with an oligonucleotide, which achieves the detection of the expression of CAR by means of single cell sequencing.
Inventors:
HUANG JUN (CN)
HU YIFEI (CN)
HU YIFEI (CN)
Application Number:
PCT/CN2022/127159
Publication Date:
May 04, 2023
Filing Date:
October 24, 2022
Export Citation:
Assignee:
HUANG JUN (CN)
International Classes:
C12N5/10; C12Q1/6869; G01N33/569; G01N33/58
Foreign References:
| CN111246861A | 2020-06-05 | |||
| CN111100199A | 2020-05-05 | |||
| CN107847544A | 2018-03-27 | |||
| CN108474002A | 2018-08-31 | |||
| CN112153989A | 2020-12-29 |
Other References:
SHARMA PREETI, MARADA VENKATA V. V. R., CAI QI, KIZERWETTER MONIKA, HE YANRAN, WOLF STEVEN P., SCHREIBER KARIN, CLAUSEN HENRIK, SC: "Structure-guided engineering of the affinity and specificity of CARs against Tn-glycopeptides", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 117, no. 26, 30 June 2020 (2020-06-30), pages 15148 - 15159, XP093060089, ISSN: 0027-8424, DOI: 10.1073/pnas.1920662117
ROMÁN-MELÉNDEZ GABRIEL D., VENKATARAMAN THIAGARAJAN, MONACO DANIEL R., LARMAN H. BENJAMIN: "Protease Activity Profiling Via Programmable Phage Display", BIORXIV, 12 May 2020 (2020-05-12), XP093060095, DOI: 10.1101/2020.05.11.089607
ROMÁN-MELÉNDEZ GABRIEL D., VENKATARAMAN THIAGARAJAN, MONACO DANIEL R., LARMAN H. BENJAMIN: "Protease Activity Profiling Via Programmable Phage Display", BIORXIV, 12 May 2020 (2020-05-12), XP093060095, DOI: 10.1101/2020.05.11.089607
Attorney, Agent or Firm:
SOON IP LAW FIRM (CN)
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